Restriction map for
pFN21AE4234
(Circular) (Six-base) MAPSORT of: pFN21AE4234.seq Check: 4867 from: 1 to: 7267
pFN21AE4234 7267 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5077 278
Size: 53 83 186 936 3541 2468
Fragments arranged by size:
3541 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4610 1937
Size: 2673 4594
Acc65I G'GTAC_C
Cuts at: 4588 4588
Size: 7267
AclI AA'CG_TT
Cuts at: 1563 5394 5767 1563
Size: 3831 373 3063
Fragments arranged by size:
3831 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 2117 3045 4034 4340 4408 5330 6714
Size: 482 3 928 989 306 68 922 1384
Cuts at: 6714 6982 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 989 928 922 482 306 268
68 3
AflII C'TTAA_G
Cuts at: 820 1017 3329 820
Size: 197 2312 4758
Fragments arranged by size:
4758 2312 197
AflIII A'CryG_T
Cuts at: 1203 2652 4162 4452 6166 7143 1203
Size: 1449 1510 290 1714 977 1327
Fragments arranged by size:
1714 1510 1449 1327 977 290
AgeI A'CCGG_T
Cuts at: 6098 6098
Size: 7267
AhdI GACnn_n'nnGTC
Cuts at: 4309 5996 4309
Size: 1687 5580
AleI CACnn'nnGTG
Cuts at: 1166 2301 2618 1166
Size: 1135 317 5815
Fragments arranged by size:
5815 1135 317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2167 3271 4522 1184
Size: 983 1104 1251 3929
Fragments arranged by size:
3929 1251 1104 983
AlwNI CAG_nnn'CTG
Cuts at: 2670 5010 6582 2670
Size: 2340 1572 3355
Fragments arranged by size:
3355 2340 1572
ApaLI G'TGCA_C
Cuts at: 2399 5324 6480 2399
Size: 2925 1156 3186
Fragments arranged by size:
3186 2925 1156
ApoI r'AATT_y
Cuts at: 1493 2357 2807 3264 3308 3404 3735 4058 4239
Size: 864 450 457 44 96 331 323 181
Cuts at: 4239 4263 4576 4802 4954 7063 1493
Size: 24 313 226 152 2109 1697
Fragments arranged by size:
2109 1697 864 457 450 331 323 313
226 181 152 96 44 24
AseI AT'TA_AT
Cuts at: 160 1032 3680 5821 160
Size: 872 2648 2141 1606
Fragments arranged by size:
2648 2141 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7267
AvaI C'yCGr_G
Cuts at: 1870 1943 2625 4592 1870
Size: 73 682 1967 4545
Fragments arranged by size:
4545 1967 682 73
BamHI G'GATC_C
Cuts at: 4597 4964 4597
Size: 367 6900
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4588 6043 618
Size: 325 217 627 2801 1455 1842
Fragments arranged by size:
2801 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4586 1391
Size: 3195 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2633 3774 4429 928
Size: 971 734 1141 655 3766
Fragments arranged by size:
3766 1141 971 734 655
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5458 5492 1903
Size: 34 3521 34 3678
Fragments arranged by size:
3678 3521 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2667 5159 6369 2667
Size: 2492 1210 3565
Fragments arranged by size:
3565 2492 1210
BclI T'GATC_A
Cuts at: 1548 2839 3094 1548
Size: 1291 255 5721
Fragments arranged by size:
5721 1291 255
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5878 136
Size: 107 122 71 631 605 4206 1525
Fragments arranged by size:
4206 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2952 3460 3939 7262 2952
Size: 508 479 3323 2957
Fragments arranged by size:
3323 2957 508 479
BlpI GC'TnA_GC
Cuts at: 4416 4684 4416
Size: 268 6999
Bme1580I G_kGCm'C
Cuts at: 1790 2189 2403 2625 4012 5328 6484 1790
Size: 399 214 222 1387 1316 1156 2573
Fragments arranged by size:
2573 1387 1316 1156 399 222 214
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7267
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2040 4998 5956
Size: 416 53 83 399 24 623 2958 958
Cuts at: 5956 442
Size: 1753
Fragments arranged by size:
2958 1753 958 623 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2151 1056
Size: 1095 6172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2370 3477 5927 1239
Size: 1131 1107 2450 2579
Fragments arranged by size:
2579 2450 1131 1107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3956 5392 6257 6555 3956
Size: 1436 865 298 4668
Fragments arranged by size:
4668 1436 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3078 3810 5930 882
Size: 617 1579 732 2120 2219
Fragments arranged by size:
2219 2120 1579 732 617
BsaAI yAC'GTr
Cuts at: 493 1204 5058 493
Size: 711 3854 2702
Fragments arranged by size:
3854 2702 711
BsaBI GATnn'nnATC
Cuts at: 7261 7261
Size: 7267
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5074 5456 6091 275
Size: 53 83 186 936 3541 382 635 1451
Fragments arranged by size:
3541 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2441 5700 6098 6372 6519 6930 2441
Size: 3259 398 274 147 411 2778
Fragments arranged by size:
3259 2778 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7042
Fragments arranged by size:
7042 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 3243 4379 1186
Size: 847 1210 1136 4074
Fragments arranged by size:
4074 1210 1136 847
BseYI C'CCAG_C
Cuts at: 2703 4481 6470 6966 2703
Size: 1778 1989 496 3004
Fragments arranged by size:
3004 1989 1778 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2348 4482 1163
Size: 573 612 2134 3948
Fragments arranged by size:
3948 2134 612 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4717 5478 6506 1540
Size: 275 186 2716 761 1028 2301
Fragments arranged by size:
2716 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2298 2403 2729 5328 5413 6484 2298
Size: 105 326 2599 85 1071 3081
Fragments arranged by size:
3081 2599 1071 326 105 85
BsmI GAATG_Cn'
Cuts at: 4778 4871 4778
Size: 93 7174
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2189 2298 2403 2625 2729 4012 4586
Size: 399 399 109 105 222 104 1283 574
Cuts at: 4586 5328 5413 6484 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1283 1071 742 574 399 399 222
109 105 104 85
BspEI T'CCGG_A
Cuts at: 6930 6930
Size: 7267
BspHI T'CATG_A
Cuts at: 5156 6886 5156
Size: 1730 5537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2320 4622 844
Size: 1476 2302 3489
Fragments arranged by size:
3489 2302 1476
BsrBI CCG'CTC
Cuts at: 1394 1823 5154 1394
Size: 429 3331 3507
Fragments arranged by size:
3507 3331 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3586 3913 4441 5762 5936 65
Size: 1183 2338 327 528 1321 174 1396
Fragments arranged by size:
2338 1396 1321 1183 528 327 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4446 5911 6098 1700
Size: 110 2636 1465 187 2869
Fragments arranged by size:
2869 2636 1465 187 110
BsrGI T'GTAC_A
Cuts at: 96 3555 96
Size: 3459 3808
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7267
BssSI C'ACGA_G
Cuts at: 2363 5327 6339 2363
Size: 2964 1012 3291
Fragments arranged by size:
3291 2964 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2670 4267 4515 794
Size: 1876 1597 248 3546
Fragments arranged by size:
3546 1876 1597 248
BstBI TT'CG_AA
Cuts at: 6071 6071
Size: 7267
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7267
BstXI CCAn_nnnn'nTGG
Cuts at: 2572 2572
Size: 7267
BstYI r'GATC_y
Cuts at: 1972 2417 2952 3460 3939 4209 4597 4964 5349
Size: 445 535 508 479 270 388 367 385
Cuts at: 5349 5366 6818 6904 6916 7262 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 535 508 479 445 388 385
367 346 270 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 4531 513
Size: 552 939 2527 3249
Fragments arranged by size:
3249 2527 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6528
Fragments arranged by size:
6528 683 56
BtsI GCAGTG_nn'
Cuts at: 4525 4783 5577 5597 4525
Size: 258 794 20 6195
Fragments arranged by size:
6195 794 258 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7267
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7232
DraI TTT'AAA
Cuts at: 3313 4571 3313
Size: 1258 6009
DraIII CAC_nnn'GTG
Cuts at: 3220 3220
Size: 7267
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6274 809
Size: 507 4958 1802
Fragments arranged by size:
4958 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3072 4714 5603
Size: 54 1425 50 275 18 1242 1642 889
Cuts at: 5603 7232 8
Size: 1629 43
Fragments arranged by size:
1642 1629 1425 1242 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4714 1537
Size: 275 2902 4090
Fragments arranged by size:
4090 2902 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 2073 2943 2962 3960 5197 1353
Size: 711 9 870 19 998 1237 3423
Fragments arranged by size:
3423 1237 998 870 711 19 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5854 6228 6374 387
Size: 1064 138 435 3830 374 146 1280
Fragments arranged by size:
3830 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2117 2370 3045 3477 4034 4340
Size: 393 482 3 253 675 432 557 306
Cuts at: 4340 4408 5330 5927 6714 6982 1239
Size: 68 922 597 787 268 1524
Fragments arranged by size:
1524 922 787 675 597 557 482 432
393 306 268 253 68 3
EcoO109I rG'GnC_Cy
Cuts at: 2034 2034
Size: 7267
EcoRI G'AATT_C
Cuts at: 2807 4576 4954 2807
Size: 1769 378 5120
Fragments arranged by size:
5120 1769 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4370 4370
Size: 7267
FspI TGC'GCA
Cuts at: 5773 5773
Size: 7267
HaeII r_GCGC'y
Cuts at: 1678 6414 1678
Size: 4736 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2533 2565 2582 2614 2904
Size: 32 135 32 961 32 17 32 290
Cuts at: 2904 2936 3961 3993 4295 4327 5908 5940 5982
Size: 32 1025 32 302 32 1581 32 42
Cuts at: 5982 6014 1373
Size: 32 2626
Fragments arranged by size:
2626 1581 1025 961 302 290 135 42
32 32 32 32 32 32 32 32
32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 3709 3947 4168 4611 4857 669
Size: 988 281 1771 238 221 443 246 3079
Fragments arranged by size:
3079 1771 988 443 281 246 238 221
HindIII A'AGCT_T
Cuts at: 748 3803 748
Size: 3055 4212
HpaI GTT'AAC
Cuts at: 4857 4857
Size: 7267
KpnI G_GTAC'C
Cuts at: 4592 4592
Size: 7267
MfeI C'AATT_G
Cuts at: 4866 4866
Size: 7267
MluI A'CGCG_T
Cuts at: 7143 7143
Size: 7267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6381 6565 1305
Size: 5076 184 2007
Fragments arranged by size:
5076 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3074 10
Size: 54 1425 343 1242 4203
Fragments arranged by size:
4203 1425 1242 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2301 2303 2618 2748 3253 5225 5584
Size: 648 1135 2 315 130 505 1972 359
Cuts at: 5584 5743 518
Size: 159 2042
Fragments arranged by size:
2042 1972 1135 648 505 359 315 159
130 2
MspA1I CmG'CkG
Cuts at: 1606 3149 4683 5360 6508 6753 1606
Size: 1543 1534 677 1148 245 2120
Fragments arranged by size:
2120 1543 1534 1148 677 245
NaeI GCC'GGC
Cuts at: 4448 4448
Size: 7267
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5776
Fragments arranged by size:
5776 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7267
NgoMIV G'CCGG_C
Cuts at: 4446 4446
Size: 7267
NheI G'CTAG_C
Cuts at: 1052 2147 1052
Size: 1095 6172
NotI GC'GGCC_GC
Cuts at: 4714 4714
Size: 7267
NspI r_CATG'y
Cuts at: 1132 1229 2656 2747 3715 3791 4166 4456 4625
Size: 97 1427 91 968 76 375 290 169
Cuts at: 4625 6170 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1427 968 375 290 169 97
91 76
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7267
PciI A'CATG_T
Cuts at: 2652 4162 4452 6166 2652
Size: 1510 290 1714 3753
Fragments arranged by size:
3753 1714 1510 290
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7267
PmeI GTTT'AAAC
Cuts at: 4571 4571
Size: 7267
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5336 5368 6849 6881 5336
Size: 32 1481 32 5722
Fragments arranged by size:
5722 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2034 2034
Size: 7267
PshAI GACnn'nnGTC
Cuts at: 1613 2714 1613
Size: 1101 6166
PsiI TTA'TAA
Cuts at: 3306 4837 3306
Size: 1531 5736
PstI C_TGCA'G
Cuts at: 830 2666 4520 4619 830
Size: 1836 1854 99 3478
Fragments arranged by size:
3478 1854 1836 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7267
PvuII CAG'CTG
Cuts at: 3149 3149
Size: 7267
SalI G'TCGA_C
Cuts at: 1936 4609 1936
Size: 2673 4594
SanDI GG'GwC_CC
Cuts at: 2034 2034
Size: 7267
SbfI CC_TGCA'GG
Cuts at: 4619 4619
Size: 7267
ScaI AGT'ACT
Cuts at: 5515 7227 5515
Size: 1712 5555
SexAI A'CCwGG_T
Cuts at: 3216 3216
Size: 7267
SfcI C'TryA_G
Cuts at: 826 1045 2049 2662 3782 4516 4615 5750 6431
Size: 219 1004 613 1120 734 99 1135 681
Cuts at: 6431 6622 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1120 1004 734 681 613 219
191 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7267
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7267
SmaI CCC'GGG
Cuts at: 1872 2627 4594 1872
Size: 755 1967 4545
Fragments arranged by size:
4545 1967 755
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3329 3935 5371 6272 6534 820
Size: 197 926 1386 606 1436 901 262 1553
Fragments arranged by size:
1553 1436 1386 926 901 606 262 197
SnaBI TAC'GTA
Cuts at: 493 5058 493
Size: 4565 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7267
SphI G_CATG'C
Cuts at: 1132 2747 4625 1132
Size: 1615 1878 3774
Fragments arranged by size:
3774 1878 1615
SspI AAT'ATT
Cuts at: 5 52 3169 5191 5
Size: 47 3117 2022 2081
Fragments arranged by size:
3117 2081 2022 47
StuI AGG'CCT
Cuts at: 1517 2908 1517
Size: 1391 5876
StyI C'CwwG_G
Cuts at: 513 1065 2004 3075 4065 4230 4706 513
Size: 552 939 1071 990 165 476 3074
Fragments arranged by size:
3074 1071 990 939 552 476 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2291 2418 2564 5294 5311 5464 5649
Size: 1255 711 127 146 2730 17 153 185
Cuts at: 5649 6094 325
Size: 445 1498
Fragments arranged by size:
2730 1498 1255 711 445 185 153 146
127 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2851 3555 5513
Size: 274 80 33 51 1443 874 704 1958
Cuts at: 5513 7225 96
Size: 1712 138
Fragments arranged by size:
1958 1712 1443 874 704 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7227 7259 7227
Size: 32 7235
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7267
XbaI T'CTAG_A
Cuts at: 4603 4603
Size: 7267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2911 3266 2911
Size: 355 6912
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7267
XmaI C'CCGG_G
Cuts at: 1870 2625 4592 1870
Size: 755 1967 4545
Fragments arranged by size:
4545 1967 755
XmnI GAAnn'nnTTC
Cuts at: 2130 2358 5396 2130
Size: 228 3038 4001
Fragments arranged by size:
4001 3038 228
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5075 276
Size: 53 83 186 936 3541 2468
Fragments arranged by size:
3541 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SapI SfiI SfoI SrfI SwaI