Restriction map for
pFN21AE4233
(Circular) (Six-base) MAPSORT of: pFN21AE4233.seq Check: 570 from: 1 to: 7255
pFN21AE4233 7255 bp
With 182 enzymes: SgfI *
March 28, 2012 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4223 4223
Size: 7255
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5065 278
Size: 53 83 186 936 3529 2468
Fragments arranged by size:
3529 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3384 4598 1937
Size: 1447 1214 4594
Fragments arranged by size:
4594 1447 1214
Acc65I G'GTAC_C
Cuts at: 4576 4576
Size: 7255
AclI AA'CG_TT
Cuts at: 1563 5382 5755 1563
Size: 3819 373 3063
Fragments arranged by size:
3819 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2955 5318 6702 6970 1632
Size: 1323 2363 1384 268 1917
Fragments arranged by size:
2363 1917 1384 1323 268
AflII C'TTAA_G
Cuts at: 820 1017 4316 820
Size: 197 3299 3759
Fragments arranged by size:
3759 3299 197
AflIII A'CryG_T
Cuts at: 1203 6154 7131 1203
Size: 4951 977 1327
Fragments arranged by size:
4951 1327 977
AgeI A'CCGG_T
Cuts at: 6086 6086
Size: 7255
AhdI GACnn_n'nnGTC
Cuts at: 2923 5984 2923
Size: 3061 4194
AleI CACnn'nnGTG
Cuts at: 1166 3143 1166
Size: 1977 5278
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7255
AlwNI CAG_nnn'CTG
Cuts at: 2122 4998 6570 2122
Size: 2876 1572 2807
Fragments arranged by size:
2876 2807 1572
ApaI G_GGCC'C
Cuts at: 2609 2609
Size: 7255
ApaLI G'TGCA_C
Cuts at: 3815 5312 6468 3815
Size: 1497 1156 4602
Fragments arranged by size:
4602 1497 1156
ApoI r'AATT_y
Cuts at: 1493 2149 2203 2273 3199 3707 3768 3877 4287
Size: 656 54 70 926 508 61 109 410
Cuts at: 4287 4564 4790 4942 7051 1493
Size: 277 226 152 2109 1697
Fragments arranged by size:
2109 1697 926 656 508 410 277 226
152 109 70 61 54
AseI AT'TA_AT
Cuts at: 160 1032 5809 160
Size: 872 4777 1606
Fragments arranged by size:
4777 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7255
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 4580 1870
Size: 73 132 2505 4545
Fragments arranged by size:
4545 2505 132 73
AvrII C'CTAG_G
Cuts at: 2809 2809
Size: 7255
BamHI G'GATC_C
Cuts at: 4585 4952 4585
Size: 367 6888
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2047 4576 6031 618
Size: 325 217 627 230 30 2529 1455 1842
Fragments arranged by size:
2529 1842 1455 627 325 230 217 30
BanII G_rGCy'C
Cuts at: 1391 2076 2609 3850 3918 4007 4574 1391
Size: 685 533 1241 68 89 567 4072
Fragments arranged by size:
4072 1241 685 567 533 89 68
BbeI G_GCGC'C
Cuts at: 2021 2051 2021
Size: 30 7225
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2388 2636 2961 4275 928
Size: 971 489 248 325 1314 3908
Fragments arranged by size:
3908 1314 971 489 325 248
BbvCI CC'TCA_GC
Cuts at: 3037 3037
Size: 7255
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3879 3913 5446 5480 1903
Size: 34 1942 34 1533 34 3678
Fragments arranged by size:
3678 1942 1533 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3160 5147 6357 3160
Size: 1987 1210 4058
Fragments arranged by size:
4058 1987 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7255
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2127 5866
Size: 107 122 71 631 605 337 118 3739
Cuts at: 5866 136
Size: 1525
Fragments arranged by size:
3739 1525 631 605 337 122 118 107
71
BglII A'GATC_T
Cuts at: 2133 2347 7250 2133
Size: 214 4903 2138
Fragments arranged by size:
4903 2138 214
BlpI GC'TnA_GC
Cuts at: 4672 4672
Size: 7255
Bme1580I G_kGCm'C
Cuts at: 1790 2609 3252 3819 5316 6472 1790
Size: 819 643 567 1497 1156 2573
Fragments arranged by size:
2573 1497 1156 819 643 567
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7255
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2716 3959 4986
Size: 416 53 83 399 24 1299 1243 1027
Cuts at: 4986 5944 442
Size: 958 1753
Fragments arranged by size:
1753 1299 1243 1027 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7255
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5915 1239
Size: 4676 2579
Bpu10I CC'TnA_GC
Cuts at: 3037 3037
Size: 7255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3834 5380 6245 6543 3834
Size: 1546 865 298 4546
Fragments arranged by size:
4546 1546 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2931 5918 882
Size: 617 1432 2987 2219
Fragments arranged by size:
2987 2219 1432 617
BsaAI yAC'GTr
Cuts at: 493 1204 2962 5046 493
Size: 711 1758 2084 2702
Fragments arranged by size:
2702 2084 1758 711
BsaBI GATnn'nnATC
Cuts at: 7249 7249
Size: 7255
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2048 5062 5444
Size: 53 83 186 936 485 30 3014 382
Cuts at: 5444 6079 275
Size: 635 1451
Fragments arranged by size:
3014 1451 936 635 485 382 186 83
53 30
BsaWI w'CCGG_w
Cuts at: 5688 6086 6360 6507 6918 5688
Size: 398 274 147 411 6025
Fragments arranged by size:
6025 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3333 3363 1178
Size: 30 165 30 1930 30 5070
Fragments arranged by size:
5070 1930 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2753 3289 1186
Size: 1567 536 5152
Fragments arranged by size:
5152 1567 536
BseYI C'CCAG_C
Cuts at: 3654 6458 6954 3654
Size: 2804 496 3955
Fragments arranged by size:
3955 2804 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3095 1163
Size: 573 1359 5323
Fragments arranged by size:
5323 1359 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4705 5466 6494 1540
Size: 275 186 2704 761 1028 2301
Fragments arranged by size:
2704 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3819 3850 3918 4007 5316 5401 6472 3819
Size: 31 68 89 1309 85 1071 4602
Fragments arranged by size:
4602 1309 1071 89 85 68 31
BsmI GAATG_Cn'
Cuts at: 4766 4859 4766
Size: 93 7162
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2609 3252 3819 3850 3918 4007
Size: 399 286 533 643 567 31 68 89
Cuts at: 4007 4574 5316 5401 6472 1391
Size: 567 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 643 567 567 533 399
286 89 85 68 31
BspEI T'CCGG_A
Cuts at: 6918 6918
Size: 7255
BspHI T'CATG_A
Cuts at: 4477 5144 6874 4477
Size: 667 1730 4858
Fragments arranged by size:
4858 1730 667
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4223 4610 844
Size: 3379 387 3489
Fragments arranged by size:
3489 3379 387
BsrBI CCG'CTC
Cuts at: 1394 1823 5142 1394
Size: 429 3319 3507
Fragments arranged by size:
3507 3319 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5750 5924 65
Size: 1183 4502 174 1396
Fragments arranged by size:
4502 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5899 6086 1700
Size: 110 4089 187 2869
Fragments arranged by size:
4089 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2527 96
Size: 2431 4824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7255
BssSI C'ACGA_G
Cuts at: 5315 6327 5315
Size: 1012 6243
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2122 794
Size: 1328 5927
BstBI TT'CG_AA
Cuts at: 6059 6059
Size: 7255
BstEII G'GTnAC_C
Cuts at: 1184 2689 1184
Size: 1505 5750
BstXI CCAn_nnnn'nTGG
Cuts at: 2206 3145 2206
Size: 939 6316
BstYI r'GATC_y
Cuts at: 1972 2133 2347 2696 4499 4585 4952 5337 5354
Size: 161 214 349 1803 86 367 385 17
Cuts at: 5354 6806 6892 6904 7250 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1803 1452 385 367 349 346 214
161 86 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2545 513
Size: 552 939 541 5223
Fragments arranged by size:
5223 939 552 541
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6516
Fragments arranged by size:
6516 683 56
BtsI GCAGTG_nn'
Cuts at: 3138 3519 4771 5565 5585 3138
Size: 381 1252 794 20 4808
Fragments arranged by size:
4808 1252 794 381 20
ClaI AT'CG_AT
Cuts at: 1554 3318 1554
Size: 1764 5491
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7220
DraI TTT'AAA
Cuts at: 4559 4559
Size: 7255
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6262 809
Size: 507 4946 1802
Fragments arranged by size:
4946 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2566 4702 5591
Size: 54 1425 50 275 18 736 2136 889
Cuts at: 5591 7220 8
Size: 1629 43
Fragments arranged by size:
2136 1629 1425 889 736 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4702 1537
Size: 275 2890 4090
Fragments arranged by size:
4090 2890 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2730 2934 3993 4329 5185 1353
Size: 1377 204 1059 336 856 3423
Fragments arranged by size:
3423 1377 1059 856 336 204
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5842 6216 6362 387
Size: 1064 138 4253 374 146 1280
Fragments arranged by size:
4253 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3848 3916 4005 3848
Size: 68 89 7098
Fragments arranged by size:
7098 89 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2955 5318 5915 6702 6970 1239
Size: 393 1323 2363 597 787 268 1524
Fragments arranged by size:
2363 1524 1323 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2606 2606
Size: 7255
EcoRI G'AATT_C
Cuts at: 4564 4942 4564
Size: 378 6877
EcoRV GAT'ATC
Cuts at: 3445 3445
Size: 7255
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2483 2483
Size: 7255
FspI TGC'GCA
Cuts at: 5761 5761
Size: 7255
HaeII r_GCGC'y
Cuts at: 1678 2021 2051 6402 1678
Size: 343 30 4351 2531
Fragments arranged by size:
4351 2531 343 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2909 2941 3457 3489 5896
Size: 32 135 32 1337 32 516 32 2407
Cuts at: 5896 5928 5970 6002 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2407 1337 516 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2434 4599 4845 669
Size: 988 281 496 2165 246 3079
Fragments arranged by size:
3079 2165 988 496 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7255
HpaI GTT'AAC
Cuts at: 4845 4845
Size: 7255
KasI G'GCGC_C
Cuts at: 2017 2047 2017
Size: 30 7225
KpnI G_GTAC'C
Cuts at: 4580 4580
Size: 7255
MfeI C'AATT_G
Cuts at: 3402 4536 4854 3402
Size: 1134 318 5803
Fragments arranged by size:
5803 1134 318
MluI A'CGCG_T
Cuts at: 7131 7131
Size: 7255
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6369 6553 1305
Size: 5064 184 2007
Fragments arranged by size:
5064 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2568 10
Size: 54 1425 343 736 4697
Fragments arranged by size:
4697 1425 736 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3143 3434 3594 3700 5213 5572 5731
Size: 648 1977 291 160 106 1513 359 159
Cuts at: 5731 518
Size: 2042
Fragments arranged by size:
2042 1977 1513 648 359 291 160 159
106
MspA1I CmG'CkG
Cuts at: 1606 2054 3041 4671 5348 6496 6741 1606
Size: 448 987 1630 677 1148 245 2120
Fragments arranged by size:
2120 1630 1148 987 677 448 245
NarI GG'CG_CC
Cuts at: 2018 2048 2018
Size: 30 7225
NcoI C'CATG_G
Cuts at: 513 1065 2004 2545 513
Size: 552 939 541 5223
Fragments arranged by size:
5223 939 552 541
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7255
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7255
NotI GC'GGCC_GC
Cuts at: 4702 4702
Size: 7255
NruI TCG'CGA
Cuts at: 2100 2100
Size: 7255
NspI r_CATG'y
Cuts at: 1132 1229 2544 4613 6158 1132
Size: 97 1315 2069 1545 2229
Fragments arranged by size:
2229 2069 1545 1315 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7255
PciI A'CATG_T
Cuts at: 6154 6154
Size: 7255
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2165 2239 4541 1108
Size: 1057 74 2302 3822
Fragments arranged by size:
3822 2302 1057 74
PmeI GTTT'AAAC
Cuts at: 4559 4559
Size: 7255
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3604 3636 5324 5356 6837 6869 3604
Size: 32 1688 32 1481 32 3990
Fragments arranged by size:
3990 1688 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7255
PsiI TTA'TAA
Cuts at: 4825 4825
Size: 7255
PspOMI G'GGCC_C
Cuts at: 2605 2605
Size: 7255
PstI C_TGCA'G
Cuts at: 830 2071 3514 4112 4234 4246 4326 4607 830
Size: 1241 1443 598 122 12 80 281 3478
Fragments arranged by size:
3478 1443 1241 598 281 122 80 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7255
PvuII CAG'CTG
Cuts at: 3041 3041
Size: 7255
SacI G_AGCT'C
Cuts at: 3850 3918 4007 3850
Size: 68 89 7098
Fragments arranged by size:
7098 89 68
SalI G'TCGA_C
Cuts at: 1936 4597 1936
Size: 2661 4594
SbfI CC_TGCA'GG
Cuts at: 4607 4607
Size: 7255
ScaI AGT'ACT
Cuts at: 2978 5503 7215 2978
Size: 2525 1712 3018
Fragments arranged by size:
3018 2525 1712
SfcI C'TryA_G
Cuts at: 826 1045 2067 2876 3510 3546 4108 4230 4242
Size: 219 1022 809 634 36 562 122 12
Cuts at: 4242 4322 4603 5738 6419 6610 826
Size: 80 281 1135 681 191 1471
Fragments arranged by size:
1471 1135 1022 809 681 634 562 281
219 191 122 80 36 12
SfoI GGC'GCC
Cuts at: 2019 2049 2019
Size: 30 7225
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7255
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7255
SmaI CCC'GGG
Cuts at: 1872 4582 1872
Size: 2710 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3849 4316 5359 6260 6522 820
Size: 197 926 1906 467 1043 901 262 1553
Fragments arranged by size:
1906 1553 1043 926 901 467 262 197
SnaBI TAC'GTA
Cuts at: 493 5046 493
Size: 4553 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7255
SphI G_CATG'C
Cuts at: 1132 2544 4613 1132
Size: 1412 2069 3774
Fragments arranged by size:
3774 2069 1412
SspI AAT'ATT
Cuts at: 5 52 5179 5
Size: 47 5127 2081
Fragments arranged by size:
5127 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7255
StyI C'CwwG_G
Cuts at: 513 1065 2004 2545 2809 4694 513
Size: 552 939 541 264 1885 3074
Fragments arranged by size:
3074 1885 939 552 541 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5282 5299 5452 5637 6082 325
Size: 1255 3702 17 153 185 445 1498
Fragments arranged by size:
3702 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2527 2976 4226
Size: 274 80 33 51 1443 550 449 1250
Cuts at: 4226 5501 7213 96
Size: 1275 1712 138
Fragments arranged by size:
1712 1443 1275 1250 550 449 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4044 4076 7215 7247 4044
Size: 32 3139 32 4052
Fragments arranged by size:
4052 3139 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7255
XbaI T'CTAG_A
Cuts at: 4591 4591
Size: 7255
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7255
XmaI C'CCGG_G
Cuts at: 1870 4580 1870
Size: 2710 4545
XmnI GAAnn'nnTTC
Cuts at: 2848 4291 5384 2848
Size: 1443 1093 4719
Fragments arranged by size:
4719 1443 1093
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5063 276
Size: 53 83 186 936 3529 2468
Fragments arranged by size:
3529 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI NgoMIV
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI