Restriction map for
pFN21AE4230
(Circular) (Six-base) MAPSORT of: pFN21AE4230.seq Check: 6602 from: 1 to: 7129
pFN21AE4230 7129 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4939 278
Size: 53 83 186 936 3403 2468
Fragments arranged by size:
3403 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3465 4472 1937
Size: 1528 1007 4594
Fragments arranged by size:
4594 1528 1007
Acc65I G'GTAC_C
Cuts at: 4450 4450
Size: 7129
AclI AA'CG_TT
Cuts at: 1563 5256 5629 1563
Size: 3693 373 3063
Fragments arranged by size:
3693 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2023 2603 2898 2916 3333 3471 5192 6576
Size: 391 580 295 18 417 138 1721 1384
Cuts at: 6576 6844 1632
Size: 268 1917
Fragments arranged by size:
1917 1721 1384 580 417 391 295 268
138 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6932
AflIII A'CryG_T
Cuts at: 1203 6028 7005 1203
Size: 4825 977 1327
Fragments arranged by size:
4825 1327 977
AgeI A'CCGG_T
Cuts at: 5960 5960
Size: 7129
AhdI GACnn_n'nnGTC
Cuts at: 5858 5858
Size: 7129
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2526 4302 1184
Size: 1342 1776 4011
Fragments arranged by size:
4011 1776 1342
AlwNI CAG_nnn'CTG
Cuts at: 2056 3869 3914 4872 6444 2056
Size: 1813 45 958 1572 2741
Fragments arranged by size:
2741 1813 1572 958 45
ApaLI G'TGCA_C
Cuts at: 3609 5186 6342 3609
Size: 1577 1156 4396
Fragments arranged by size:
4396 1577 1156
ApoI r'AATT_y
Cuts at: 1493 2995 3349 3403 3617 4237 4375 4438 4664
Size: 1502 354 54 214 620 138 63 226
Cuts at: 4664 4816 6925 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1502 620 354 226 214 152
138 63 54
AseI AT'TA_AT
Cuts at: 160 1032 5683 160
Size: 872 4651 1606
Fragments arranged by size:
4651 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
AvaI C'yCGr_G
Cuts at: 1870 1943 2467 2671 2758 3134 4454 1870
Size: 73 524 204 87 376 1320 4545
Fragments arranged by size:
4545 1320 524 376 204 87 73
BamHI G'GATC_C
Cuts at: 4459 4826 4459
Size: 367 6762
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2518 3299 4121 4450 5905
Size: 325 217 627 731 781 822 329 1455
Cuts at: 5905 618
Size: 1842
Fragments arranged by size:
1842 1455 822 781 731 627 329 325
217
BanII G_rGCy'C
Cuts at: 1391 3051 3203 3231 3673 4448 1391
Size: 1660 152 28 442 775 4072
Fragments arranged by size:
4072 1660 775 442 152 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2832 4136 928
Size: 971 933 1304 3921
Fragments arranged by size:
3921 1304 971 933
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5320 5354 1903
Size: 34 3383 34 3678
Fragments arranged by size:
3678 3383 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3311 5021 6231 3311
Size: 1710 1210 4209
Fragments arranged by size:
4209 1710 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7129
BfrBI ATG'CAT
Cuts at: 3436 3436
Size: 7129
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5740 136
Size: 107 122 71 631 605 4068 1525
Fragments arranged by size:
4068 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3685 4114 7124 3685
Size: 429 3010 3690
Fragments arranged by size:
3690 3010 429
BlpI GC'TnA_GC
Cuts at: 4546 4546
Size: 7129
Bme1580I G_kGCm'C
Cuts at: 1790 3613 5190 6346 1790
Size: 1823 1577 1156 2573
Fragments arranged by size:
2573 1823 1577 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7129
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2884 3045 3593
Size: 416 53 83 399 24 1467 161 548
Cuts at: 3593 4860 5818 442
Size: 1267 958 1753
Fragments arranged by size:
1753 1467 1267 958 548 416 399 161
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3532 3973 3532
Size: 441 6688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2367 3968 4046 5789 1239
Size: 1128 1601 78 1743 2579
Fragments arranged by size:
2579 1743 1601 1128 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2724 3908 5254 6119 6417 2724
Size: 1184 1346 865 298 3436
Fragments arranged by size:
3436 1346 1184 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2118 2661 3789 5792 882
Size: 617 619 543 1128 2003 2219
Fragments arranged by size:
2219 2003 1128 619 617 543
BsaAI yAC'GTr
Cuts at: 493 1204 4920 493
Size: 711 3716 2702
Fragments arranged by size:
3716 2702 711
BsaBI GATnn'nnATC
Cuts at: 7123 7123
Size: 7129
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4936 5318 5953 275
Size: 53 83 186 936 3403 382 635 1451
Fragments arranged by size:
3403 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5562 5960 6234 6381 6792 5562
Size: 398 274 147 411 5899
Fragments arranged by size:
5899 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3259 3289 1178
Size: 30 165 30 1856 30 5018
Fragments arranged by size:
5018 1856 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2240 2449 3727 3965 3977 3980 1186
Size: 1054 209 1278 238 12 3 4335
Fragments arranged by size:
4335 1278 1054 238 209 12 3
BseYI C'CCAG_C
Cuts at: 2369 3110 6332 6828 2369
Size: 741 3222 496 2670
Fragments arranged by size:
3222 2670 741 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6556
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4579 5340 6368 1540
Size: 275 186 2578 761 1028 2301
Fragments arranged by size:
2578 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2680 3613 5190 5275 6346 2680
Size: 933 1577 85 1071 3463
Fragments arranged by size:
3463 1577 1071 933 85
BsmI GAATG_Cn'
Cuts at: 3436 4640 4733 3436
Size: 1204 93 5832
Fragments arranged by size:
5832 1204 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2098 2098
Size: 7129
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2680 3051 3203 3231 3613 3673 4448
Size: 399 890 371 152 28 382 60 775
Cuts at: 4448 5190 5275 6346 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 890 775 742 399 382 371
152 85 60 28
BspEI T'CCGG_A
Cuts at: 6792 6792
Size: 7129
BspHI T'CATG_A
Cuts at: 4296 5018 6748 4296
Size: 722 1730 4677
Fragments arranged by size:
4677 1730 722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2187 4484 844
Size: 1343 2297 3489
Fragments arranged by size:
3489 2297 1343
BsrBI CCG'CTC
Cuts at: 1394 1823 3100 5016 1394
Size: 429 1277 1916 3507
Fragments arranged by size:
3507 1916 1277 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3082 3087 5624 5798 65
Size: 1183 1834 5 2537 174 1396
Fragments arranged by size:
2537 1834 1396 1183 174 5
BsrFI r'CCGG_y
Cuts at: 1700 1810 2521 5773 5960 1700
Size: 110 711 3252 187 2869
Fragments arranged by size:
3252 2869 711 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7129
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7129
BssSI C'ACGA_G
Cuts at: 5189 6201 5189
Size: 1012 6117
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4156 794
Size: 3362 3767
BstBI TT'CG_AA
Cuts at: 5933 5933
Size: 7129
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7129
BstYI r'GATC_y
Cuts at: 1972 2180 3685 4114 4459 4826 5211 5228 6680
Size: 208 1505 429 345 367 385 17 1452
Cuts at: 6680 6766 6778 7124 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1505 1452 429 385 367 346 345
208 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2432 2979 3663 2432
Size: 547 684 5898
Fragments arranged by size:
5898 684 547
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6577
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2058 2883 487
Size: 683 56 832 825 4733
Fragments arranged by size:
4733 832 825 683 56
BtsI GCAGTG_nn'
Cuts at: 2510 3187 3825 4645 5439 5459 2510
Size: 677 638 820 794 20 4180
Fragments arranged by size:
4180 820 794 677 638 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7129
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7094
DraI TTT'AAA
Cuts at: 3336 4433 3336
Size: 1097 6032
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6136 809
Size: 507 4820 1802
Fragments arranged by size:
4820 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4576 5465 7094
Size: 54 1425 50 275 18 2746 889 1629
Cuts at: 7094 8
Size: 43
Fragments arranged by size:
2746 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4576 1537
Size: 275 2764 4090
Fragments arranged by size:
4090 2764 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2116 2579 2854 2874 5059 1353
Size: 763 463 275 20 2185 3423
Fragments arranged by size:
3423 2185 763 463 275 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5716 6090 6236 387
Size: 1064 138 4127 374 146 1280
Fragments arranged by size:
4127 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2023 2367 2603 2898 2916 3333 3471
Size: 393 391 344 236 295 18 417 138
Cuts at: 3471 3968 4046 5192 5789 6576 6844 1239
Size: 497 78 1146 597 787 268 1524
Fragments arranged by size:
1524 1146 787 597 497 417 393 391
344 295 268 236 138 78 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2711 3950 2711
Size: 1239 5890
EcoRI G'AATT_C
Cuts at: 3403 4237 4438 4816 3403
Size: 834 201 378 5716
Fragments arranged by size:
5716 834 378 201
EcoRV GAT'ATC
Cuts at: 2265 2265
Size: 7129
FspI TGC'GCA
Cuts at: 5635 5635
Size: 7129
HaeII r_GCGC'y
Cuts at: 1678 6276 1678
Size: 4598 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2294 2326 2430 2462 2578
Size: 32 135 32 722 32 104 32 116
Cuts at: 2578 2610 3137 3169 3206 3238 4319 4351 5770
Size: 32 527 32 37 32 1081 32 1419
Cuts at: 5770 5802 5844 5876 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1419 1081 722 527 135 116 104
42 37 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4165 4473 4719 669
Size: 988 281 2227 308 246 3079
Fragments arranged by size:
3079 2227 988 308 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7129
HpaI GTT'AAC
Cuts at: 4719 4719
Size: 7129
KpnI G_GTAC'C
Cuts at: 4454 4454
Size: 7129
MfeI C'AATT_G
Cuts at: 2298 4728 2298
Size: 2430 4699
MluI A'CGCG_T
Cuts at: 7005 7005
Size: 7129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6243 6427 1305
Size: 4938 184 2007
Fragments arranged by size:
4938 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5307
Fragments arranged by size:
5307 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2275 3771 4272 5087 5446 5605 518
Size: 648 1109 1496 501 815 359 159 2042
Fragments arranged by size:
2042 1496 1109 815 648 501 359 159
MspA1I CmG'CkG
Cuts at: 1606 4545 5222 6370 6615 1606
Size: 2939 677 1148 245 2120
Fragments arranged by size:
2939 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6577
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7129
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7129
NotI GC'GGCC_GC
Cuts at: 4576 4576
Size: 7129
NsiI A_TGCA'T
Cuts at: 3438 3438
Size: 7129
NspI r_CATG'y
Cuts at: 1132 1229 2068 3776 4487 6032 1132
Size: 97 839 1708 711 1545 2229
Fragments arranged by size:
2229 1708 1545 839 711 97
PasI CC'CwG_GG
Cuts at: 1348 2529 1348
Size: 1181 5948
PciI A'CATG_T
Cuts at: 6028 6028
Size: 7129
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7129
PfoI T'CCnGG_A
Cuts at: 3945 4023 3945
Size: 78 7051
PmeI GTTT'AAAC
Cuts at: 4433 4433
Size: 7129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5198 5230 6711 6743 5198
Size: 32 1481 32 5584
Fragments arranged by size:
5584 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3978 1613
Size: 2365 4764
PsiI TTA'TAA
Cuts at: 4699 4699
Size: 7129
PstI C_TGCA'G
Cuts at: 830 4056 4481 830
Size: 3226 425 3478
Fragments arranged by size:
3478 3226 425
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7129
SalI G'TCGA_C
Cuts at: 1936 4471 1936
Size: 2535 4594
SbfI CC_TGCA'GG
Cuts at: 4481 4481
Size: 7129
ScaI AGT'ACT
Cuts at: 5377 7089 5377
Size: 1712 5417
SexAI A'CCwGG_T
Cuts at: 4251 4314 4251
Size: 63 7066
SfcI C'TryA_G
Cuts at: 826 1045 2784 3159 4052 4477 5612 6293 6484
Size: 219 1739 375 893 425 1135 681 191
Cuts at: 6484 826
Size: 1471
Fragments arranged by size:
1739 1471 1135 893 681 425 375 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7129
SmaI CCC'GGG
Cuts at: 1872 3136 4456 1872
Size: 1264 1320 4545
Fragments arranged by size:
4545 1320 1264
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2739 3923 5233 6134 6396 820
Size: 197 926 796 1184 1310 901 262 1553
Fragments arranged by size:
1553 1310 1184 926 901 796 262 197
SnaBI TAC'GTA
Cuts at: 493 4920 493
Size: 4427 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7129
SphI G_CATG'C
Cuts at: 1132 4487 1132
Size: 3355 3774
SspI AAT'ATT
Cuts at: 5 52 5053 5
Size: 47 5001 2081
Fragments arranged by size:
5001 2081 47
StuI AGG'CCT
Cuts at: 1517 2562 2717 3295 4155 1517
Size: 1045 155 578 860 4491
Fragments arranged by size:
4491 1045 860 578 155
StyI C'CwwG_G
Cuts at: 513 1065 2246 2722 4568 513
Size: 552 1181 476 1846 3074
Fragments arranged by size:
3074 1846 1181 552 476
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2791 3317 4160 5156 5173 5326 5511
Size: 1255 1211 526 843 996 17 153 185
Cuts at: 5511 5956 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1211 996 843 526 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5375 7087 96
Size: 274 80 33 51 1443 3398 1712 138
Fragments arranged by size:
3398 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7089 7121 7089
Size: 32 7097
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7129
XbaI T'CTAG_A
Cuts at: 4465 4465
Size: 7129
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7129
XmaI C'CCGG_G
Cuts at: 1870 3134 4454 1870
Size: 1264 1320 4545
Fragments arranged by size:
4545 1320 1264
XmnI GAAnn'nnTTC
Cuts at: 2036 5258 2036
Size: 3222 3907
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4937 276
Size: 53 83 186 936 3403 2468
Fragments arranged by size:
3403 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI