Restriction map for
pFN21AE4227
(Circular) (Six-base) MAPSORT of: pFN21AE4227.seq Check: 8269 from: 1 to: 7111
pFN21AE4227 7111 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2029 3949 2029
Size: 1920 5191
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4921 278
Size: 53 83 186 936 3385 2468
Fragments arranged by size:
3385 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3399 3864 4454 1937
Size: 1462 465 590 4594
Fragments arranged by size:
4594 1462 590 465
Acc65I G'GTAC_C
Cuts at: 4432 4432
Size: 7111
AclI AA'CG_TT
Cuts at: 1563 5238 5611 1563
Size: 3675 373 3063
Fragments arranged by size:
3675 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2144 3334 3614 3636 3863 3998 5174 6558
Size: 512 1190 280 22 227 135 1176 1384
Cuts at: 6558 6826 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1190 1176 512 280 268 227
135 22
AfeI AGC'GCT
Cuts at: 3038 3038
Size: 7111
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6914
AflIII A'CryG_T
Cuts at: 1203 4002 6010 6987 1203
Size: 2799 2008 977 1327
Fragments arranged by size:
2799 2008 1327 977
AgeI A'CCGG_T
Cuts at: 5942 5942
Size: 7111
AhdI GACnn_n'nnGTC
Cuts at: 2749 4228 5840 2749
Size: 1479 1612 4020
Fragments arranged by size:
4020 1612 1479
AleI CACnn'nnGTG
Cuts at: 1166 2558 1166
Size: 1392 5719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7111
AlwNI CAG_nnn'CTG
Cuts at: 2196 2515 2765 3262 3316 3328 3451 3520 4051
Size: 319 250 497 54 12 123 69 531
Cuts at: 4051 4854 6426 2196
Size: 803 1572 2881
Fragments arranged by size:
2881 1572 803 531 497 319 250 123
69 54 12
ApaI G_GGCC'C
Cuts at: 2954 4063 2954
Size: 1109 6002
ApaLI G'TGCA_C
Cuts at: 5168 6324 5168
Size: 1156 5955
ApoI r'AATT_y
Cuts at: 1493 2644 4420 4646 4798 6907 1493
Size: 1151 1776 226 152 2109 1697
Fragments arranged by size:
2109 1776 1697 1151 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5665 160
Size: 872 4633 1606
Fragments arranged by size:
4633 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
AvaI C'yCGr_G
Cuts at: 1870 1943 2131 2462 2556 2655 2691 2953 3278
Size: 73 188 331 94 99 36 262 325
Cuts at: 3278 4436 1870
Size: 1158 4545
Fragments arranged by size:
4545 1158 331 325 262 188 99 94
73 36
BamHI G'GATC_C
Cuts at: 4441 4808 4441
Size: 367 6744
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2324 2678 2853 3811 4300
Size: 325 217 627 537 354 175 958 489
Cuts at: 4300 4432 5887 618
Size: 132 1455 1842
Fragments arranged by size:
1842 1455 958 627 537 489 354 325
217 175 132
BanII G_rGCy'C
Cuts at: 1391 2138 2185 2532 2799 2954 3614 4063 4430
Size: 747 47 347 267 155 660 449 367
Cuts at: 4430 1391
Size: 4072
Fragments arranged by size:
4072 747 660 449 367 347 267 155
47
BbeI G_GCGC'C
Cuts at: 2857 2857
Size: 7111
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2470 3624 3630 4223 928
Size: 971 571 1154 6 593 3816
Fragments arranged by size:
3816 1154 971 593 571 6
BbvCI CC'TCA_GC
Cuts at: 4334 4334
Size: 7111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5302 5336 1903
Size: 34 3365 34 3678
Fragments arranged by size:
3678 3365 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5003 6213 5003
Size: 1210 5901
BclI T'GATC_A
Cuts at: 1548 3156 1548
Size: 1608 5503
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3638 3947 5722
Size: 107 122 71 631 605 1966 309 1775
Cuts at: 5722 136
Size: 1525
Fragments arranged by size:
1966 1775 1525 631 605 309 122 107
71
BglII A'GATC_T
Cuts at: 7106 7106
Size: 7111
BlpI GC'TnA_GC
Cuts at: 3113 3227 3951 4528 3113
Size: 114 724 577 5696
Fragments arranged by size:
5696 724 577 114
Bme1580I G_kGCm'C
Cuts at: 1790 2329 2662 2954 4063 4106 4305 5172 6328
Size: 539 333 292 1109 43 199 867 1156
Cuts at: 6328 1790
Size: 2573
Fragments arranged by size:
2573 1156 1109 867 539 333 292 199
43
BmgBI CAC'GTC
Cuts at: 1318 2905 3364 1318
Size: 1587 459 5065
Fragments arranged by size:
5065 1587 459
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2055 2132 4403
Size: 416 53 83 399 24 638 77 2271
Cuts at: 4403 4842 5800 442
Size: 439 958 1753
Fragments arranged by size:
2271 1753 958 638 439 416 399 83
77 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2582 2582
Size: 7111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2015 2363 2374 3338 3350 3479 3515 3998
Size: 776 348 11 964 12 129 36 483
Cuts at: 3998 4093 5771 1239
Size: 95 1678 2579
Fragments arranged by size:
2579 1678 964 776 483 348 129 95
36 12 11
Bpu10I CC'TnA_GC
Cuts at: 2516 2577 3722 3971 4334 2516
Size: 61 1145 249 363 5293
Fragments arranged by size:
5293 1145 363 249 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3554 3638 5236 6101 6399 3554
Size: 84 1598 865 298 4266
Fragments arranged by size:
4266 1598 865 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5774 882
Size: 617 4275 2219
Fragments arranged by size:
4275 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4003 4902 493
Size: 711 2799 899 2702
Fragments arranged by size:
2799 2702 899 711
BsaBI GATnn'nnATC
Cuts at: 7105 7105
Size: 7111
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2854 3099 4918 5300
Size: 53 83 186 936 1321 245 1819 382
Cuts at: 5300 5935 275
Size: 635 1451
Fragments arranged by size:
1819 1451 1321 936 635 382 245 186
83 53
BsaWI w'CCGG_w
Cuts at: 2681 5544 5942 6216 6363 6774 2681
Size: 2863 398 274 147 411 3018
Fragments arranged by size:
3018 2863 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2084 2114 2393 2423 3131
Size: 30 165 30 681 30 279 30 708
Cuts at: 3131 3161 3786 3816 1178
Size: 30 625 30 4473
Fragments arranged by size:
4473 708 681 625 279 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2540 2957 2960 2993 3512 4001 4010
Size: 1000 354 417 3 33 519 489 9
Cuts at: 4010 1186
Size: 4287
Fragments arranged by size:
4287 1000 519 489 417 354 33 9
3
BseYI C'CCAG_C
Cuts at: 2291 2328 2335 3014 6314 6810 2291
Size: 37 7 679 3300 496 2592
Fragments arranged by size:
3300 2592 679 496 37 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2603 3686 4175 1163
Size: 573 867 1083 489 4099
Fragments arranged by size:
4099 1083 867 573 489
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4561 5322 6350 1540
Size: 275 186 2560 761 1028 2301
Fragments arranged by size:
2560 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2522 2843 3293 5172 5257 6328 2522
Size: 321 450 1879 85 1071 3305
Fragments arranged by size:
3305 1879 1071 450 321 85
BsiWI C'GTAC_G
Cuts at: 3085 3085
Size: 7111
BsmI GAATG_Cn'
Cuts at: 4622 4715 4622
Size: 93 7018
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2185 2329 2522 2532 2662 2799
Size: 399 348 47 144 193 10 130 137
Cuts at: 2799 2843 2954 3293 3614 4063 4106 4305 4430
Size: 44 111 339 321 449 43 199 125
Cuts at: 4430 5172 5257 6328 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 449 399 348 339 321
199 193 144 137 130 125 111 85
47 44 43 10
BspEI T'CCGG_A
Cuts at: 6774 6774
Size: 7111
BspHI T'CATG_A
Cuts at: 5000 6730 5000
Size: 1730 5381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2029 3411 3949 4466 844
Size: 1185 1382 538 517 3489
Fragments arranged by size:
3489 1382 1185 538 517
BsrBI CCG'CTC
Cuts at: 1394 1823 2581 2739 2851 4998 1394
Size: 429 758 158 112 2147 3507
Fragments arranged by size:
3507 2147 758 429 158 112
BsrDI GCAATG_nn'
Cuts at: 65 1248 3682 5606 5780 65
Size: 1183 2434 1924 174 1396
Fragments arranged by size:
2434 1924 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5755 5942 1700
Size: 110 3945 187 2869
Fragments arranged by size:
3945 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3685 96
Size: 3589 3522
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7111
BssSI C'ACGA_G
Cuts at: 2775 5171 6183 2775
Size: 2396 1012 3703
Fragments arranged by size:
3703 2396 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3833 794
Size: 3039 4072
BstBI TT'CG_AA
Cuts at: 5915 5915
Size: 7111
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7111
BstXI CCAn_nnnn'nTGG
Cuts at: 2045 4393 2045
Size: 2348 4763
BstYI r'GATC_y
Cuts at: 1972 3732 4089 4441 4808 5193 5210 6662 6748
Size: 1760 357 352 367 385 17 1452 86
Cuts at: 6748 6760 7106 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1760 1452 385 367 357 352 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2392 2996 4024 513
Size: 552 939 388 604 1028 3600
Fragments arranged by size:
3600 1028 939 604 552 388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4099 487
Size: 683 56 2873 3499
Fragments arranged by size:
3499 2873 683 56
BtsI GCAGTG_nn'
Cuts at: 2058 2085 3263 3376 3815 4187 4627 5421 5441
Size: 27 1178 113 439 372 440 794 20
Cuts at: 5441 2058
Size: 3728
Fragments arranged by size:
3728 1178 794 440 439 372 113 27
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7076
DraI TTT'AAA
Cuts at: 4415 4415
Size: 7111
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2355 4130 6118 809
Size: 507 1039 1775 1988 1802
Fragments arranged by size:
1988 1802 1775 1039 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2237 2309 2491
Size: 54 1425 50 275 18 407 72 182
Cuts at: 2491 2756 2907 3105 3630 3997 4558 5447 7076
Size: 265 151 198 525 367 561 889 1629
Cuts at: 7076 8
Size: 43
Fragments arranged by size:
1629 1425 889 561 525 407 367 275
265 198 182 151 72 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4558 1537
Size: 275 2746 4090
Fragments arranged by size:
4090 2746 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2439 2629 2811 3692 3881 4314 5041 1353
Size: 1086 190 182 881 189 433 727 3423
Fragments arranged by size:
3423 1086 881 727 433 190 189 182
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2656 3919 4262 5698 6072 6218
Size: 1064 138 1067 1263 343 1436 374 146
Cuts at: 6218 387
Size: 1280
Fragments arranged by size:
1436 1280 1263 1067 1064 374 343 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2015 2144 2363 2374 3334 3338 3350
Size: 393 383 129 219 11 960 4 12
Cuts at: 3350 3479 3515 3614 3636 3863 3998 4093 5174
Size: 129 36 99 22 227 135 95 1081
Cuts at: 5174 5771 6558 6826 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1081 960 787 597 393 383 268
227 219 135 129 129 99 95 36
22 12 11 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2223 2888 3553 2223
Size: 665 665 5781
Fragments arranged by size:
5781 665 665
EcoO109I rG'GnC_Cy
Cuts at: 2208 2348 2449 2512 2573 2950 3313 3967 4239
Size: 140 101 63 61 377 363 654 272
Cuts at: 4239 2208
Size: 5080
Fragments arranged by size:
5080 654 377 363 272 140 101 63
61
EcoRI G'AATT_C
Cuts at: 2644 4420 4798 2644
Size: 1776 378 4957
Fragments arranged by size:
4957 1776 378
EcoRV GAT'ATC
Cuts at: 3076 3076
Size: 7111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3754 3754
Size: 7111
FspI TGC'GCA
Cuts at: 5617 5617
Size: 7111
HaeII r_GCGC'y
Cuts at: 1678 2383 2590 2857 2916 3040 3451 4341 6258
Size: 705 207 267 59 124 411 890 1917
Cuts at: 6258 1678
Size: 2531
Fragments arranged by size:
2531 1917 890 705 411 267 207 124
59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 2393 2425 2735
Size: 32 135 32 512 32 277 32 310
Cuts at: 2735 2767 3220 3252 4214 4246 5752 5784 5826
Size: 32 453 32 962 32 1506 32 42
Cuts at: 5826 5858 1373
Size: 32 2626
Fragments arranged by size:
2626 1506 962 512 453 310 277 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4455 4701 669
Size: 988 281 2517 246 3079
Fragments arranged by size:
3079 2517 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7111
HpaI GTT'AAC
Cuts at: 4701 4701
Size: 7111
KasI G'GCGC_C
Cuts at: 2853 2853
Size: 7111
KpnI G_GTAC'C
Cuts at: 4436 4436
Size: 7111
MfeI C'AATT_G
Cuts at: 4710 4710
Size: 7111
MluI A'CGCG_T
Cuts at: 6987 6987
Size: 7111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6225 6409 1305
Size: 4920 184 2007
Fragments arranged by size:
4920 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2239 2311 2493 2909 3107
Size: 54 1425 343 407 72 182 416 198
Cuts at: 3107 3632 10
Size: 525 3489
Fragments arranged by size:
3489 1425 525 416 407 343 198 182
72 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2558 4197 5069 5428 5587 518
Size: 648 1392 1639 872 359 159 2042
Fragments arranged by size:
2042 1639 1392 872 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2196 2277 2307 2998 3096 3325 3760 3777
Size: 590 81 30 691 98 229 435 17
Cuts at: 3777 4527 5204 6352 6597 1606
Size: 750 677 1148 245 2120
Fragments arranged by size:
2120 1148 750 691 677 590 435 245
229 98 81 30 17
NarI GG'CG_CC
Cuts at: 2854 2854
Size: 7111
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5620
Fragments arranged by size:
5620 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7111
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7111
NotI GC'GGCC_GC
Cuts at: 4558 4558
Size: 7111
NspI r_CATG'y
Cuts at: 1132 1229 4469 6014 1132
Size: 97 3240 1545 2229
Fragments arranged by size:
3240 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2203 2532 1348
Size: 855 329 5927
Fragments arranged by size:
5927 855 329
PciI A'CATG_T
Cuts at: 6010 6010
Size: 7111
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2765 1108
Size: 1657 5454
PfoI T'CCnGG_A
Cuts at: 2834 3456 4356 2834
Size: 622 900 5589
Fragments arranged by size:
5589 900 622
PmeI GTTT'AAAC
Cuts at: 4415 4415
Size: 7111
PmlI CAC'GTG
Cuts at: 4003 4003
Size: 7111
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5180 5212 6693 6725 5180
Size: 32 1481 32 5566
Fragments arranged by size:
5566 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2208 2348 2449 2573 3313 3967 4239 2208
Size: 140 101 124 740 654 272 5080
Fragments arranged by size:
5080 740 654 272 140 124 101
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7111
PsiI TTA'TAA
Cuts at: 4681 4681
Size: 7111
PspOMI G'GGCC_C
Cuts at: 2950 4059 2950
Size: 1109 6002
PstI C_TGCA'G
Cuts at: 830 3138 3312 3826 3960 3987 4463 830
Size: 2308 174 514 134 27 476 3478
Fragments arranged by size:
3478 2308 514 476 174 134 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7111
PvuII CAG'CTG
Cuts at: 2307 2307
Size: 7111
SacII CC_GC'GG
Cuts at: 2999 2999
Size: 7111
SalI G'TCGA_C
Cuts at: 1936 4453 1936
Size: 2517 4594
SanDI GG'GwC_CC
Cuts at: 2208 2348 2208
Size: 140 6971
SapI GCTCTTCn'nnn_
Cuts at: 2811 2811
Size: 7111
SbfI CC_TGCA'GG
Cuts at: 3312 4463 3312
Size: 1151 5960
ScaI AGT'ACT
Cuts at: 5359 7071 5359
Size: 1712 5399
SfcI C'TryA_G
Cuts at: 826 1045 2073 3134 3308 3822 3956 3983 4459
Size: 219 1028 1061 174 514 134 27 476
Cuts at: 4459 5594 6275 6466 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1061 1028 681 514 476 219
191 174 134 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 3638 3638
Size: 7111
SfoI GGC'GCC
Cuts at: 2855 2855
Size: 7111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7111
SmaI CCC'GGG
Cuts at: 1872 2657 2693 2955 4438 1872
Size: 785 36 262 1483 4545
Fragments arranged by size:
4545 1483 785 262 36
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3569 3653 5215 6116 6378 820
Size: 197 926 1626 84 1562 901 262 1553
Fragments arranged by size:
1626 1562 1553 926 901 262 197 84
SnaBI TAC'GTA
Cuts at: 493 4902 493
Size: 4409 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7111
SphI G_CATG'C
Cuts at: 1132 4469 1132
Size: 3337 3774
SrfI GCCC'GGGC
Cuts at: 2657 2657
Size: 7111
SspI AAT'ATT
Cuts at: 5 52 5035 5
Size: 47 4983 2081
Fragments arranged by size:
4983 2081 47
StuI AGG'CCT
Cuts at: 1517 3170 3425 3641 4310 1517
Size: 1653 255 216 669 4318
Fragments arranged by size:
4318 1653 669 255 216
StyI C'CwwG_G
Cuts at: 513 1065 2004 2224 3636 4209 4550 513
Size: 552 939 220 1412 573 341 3074
Fragments arranged by size:
3074 1412 939 573 552 341 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4209 4288 5138 5155 5308 5493 5938
Size: 1255 2629 79 850 17 153 185 445
Cuts at: 5938 325
Size: 1498
Fragments arranged by size:
2629 1498 1255 850 445 185 153 79
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3685 5357 7069
Size: 274 80 33 51 1443 1708 1672 1712
Cuts at: 7069 96
Size: 138
Fragments arranged by size:
1712 1708 1672 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3242 3274 4057 4089 7071 7103 3242
Size: 32 783 32 2982 32 3250
Fragments arranged by size:
3250 2982 783 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7111
XbaI T'CTAG_A
Cuts at: 4447 4447
Size: 7111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4390 4390
Size: 7111
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7111
XmaI C'CCGG_G
Cuts at: 1870 2655 2691 2953 4436 1870
Size: 785 36 262 1483 4545
Fragments arranged by size:
4545 1483 785 262 36
XmnI GAAnn'nnTTC
Cuts at: 5240 5240
Size: 7111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4919 276
Size: 53 83 186 936 3385 2468
Fragments arranged by size:
3385 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsmBI BstZ17I Bsu36I
DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspXI PsrI RsrII SacI SexAI SwaI