Restriction map for
pFN21AE4225
(Circular) (Six-base) MAPSORT of: pFN21AE4225.seq Check: 3623 from: 1 to: 7087
pFN21AE4225 7087 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2072 2932 2072
Size: 860 6227
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4897 278
Size: 53 83 186 936 3361 2468
Fragments arranged by size:
3361 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2571 4430 1937
Size: 634 1859 4594
Fragments arranged by size:
4594 1859 634
Acc65I G'GTAC_C
Cuts at: 4408 4408
Size: 7087
AclI AA'CG_TT
Cuts at: 1563 4386 5214 5587 1563
Size: 2823 828 373 3063
Fragments arranged by size:
3063 2823 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3548 4121 4142 5150 6534 6802 1632
Size: 1916 573 21 1008 1384 268 1917
Fragments arranged by size:
1917 1916 1384 1008 573 268 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6890
AflIII A'CryG_T
Cuts at: 1203 2259 5986 6963 1203
Size: 1056 3727 977 1327
Fragments arranged by size:
3727 1327 1056 977
AgeI A'CCGG_T
Cuts at: 5918 5918
Size: 7087
AhdI GACnn_n'nnGTC
Cuts at: 2161 3373 5816 2161
Size: 1212 2443 3432
Fragments arranged by size:
3432 2443 1212
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4011 1184
Size: 2827 4260
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2186 2218 2408 2440 2486 2518 2900 2932 2186
Size: 32 190 32 46 32 382 32 6341
Fragments arranged by size:
6341 382 190 46 32 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2386 3721 3844 4378 4830 6402 2386
Size: 1335 123 534 452 1572 3071
Fragments arranged by size:
3071 1572 1335 534 452 123
ApaI G_GGCC'C
Cuts at: 3436 4130 3436
Size: 694 6393
ApaLI G'TGCA_C
Cuts at: 5144 6300 5144
Size: 1156 5931
ApoI r'AATT_y
Cuts at: 1493 4396 4622 4774 6883 1493
Size: 2903 226 152 2109 1697
Fragments arranged by size:
2903 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5641 160
Size: 872 4609 1606
Fragments arranged by size:
4609 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7087
AvaI C'yCGr_G
Cuts at: 1870 1943 2720 2874 4412 1870
Size: 73 777 154 1538 4545
Fragments arranged by size:
4545 1538 777 154 73
BamHI G'GATC_C
Cuts at: 2265 4417 4784 2265
Size: 2152 367 4568
Fragments arranged by size:
4568 2152 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4408 5863 618
Size: 325 217 627 2621 1455 1842
Fragments arranged by size:
2621 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2015 2027 2697 2721 2727 3436 3600 4080
Size: 624 12 670 24 6 709 164 480
Cuts at: 4080 4130 4406 1391
Size: 50 276 4072
Fragments arranged by size:
4072 709 670 624 480 276 164 50
24 12 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3372 928
Size: 971 1473 4643
Fragments arranged by size:
4643 1473 971
BbvCI CC'TCA_GC
Cuts at: 2093 2093
Size: 7087
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2458 2492 5278 5312 1903
Size: 34 521 34 2786 34 3678
Fragments arranged by size:
3678 2786 521 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4979 6189 4979
Size: 1210 5877
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7087
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2054 3623 5698
Size: 107 122 71 631 605 382 1569 2075
Cuts at: 5698 136
Size: 1525
Fragments arranged by size:
2075 1569 1525 631 605 382 122 107
71
BglII A'GATC_T
Cuts at: 7082 7082
Size: 7087
BlpI GC'TnA_GC
Cuts at: 3878 4166 4504 3878
Size: 288 338 6461
Fragments arranged by size:
6461 338 288
Bme1580I G_kGCm'C
Cuts at: 1790 3436 4130 5148 6304 1790
Size: 1646 694 1018 1156 2573
Fragments arranged by size:
2573 1646 1156 1018 694
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7087
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2983 3437 4818
Size: 416 53 83 399 24 1566 454 1381
Cuts at: 4818 5776 442
Size: 958 1753
Fragments arranged by size:
1753 1566 1381 958 454 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7087
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2034 2173 2945 4348 2034
Size: 139 772 1403 4773
Fragments arranged by size:
4773 1403 772 139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2168 2270 2927 3113 3404 3416 4025
Size: 890 39 102 657 186 291 12 609
Cuts at: 4025 4322 5747 1239
Size: 297 1425 2579
Fragments arranged by size:
2579 1425 890 657 609 297 291 186
102 39 12
Bpu10I CC'TnA_GC
Cuts at: 2093 2240 2093
Size: 147 6940
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2312 2420 3521 3782 4064 5212 6077 6375 2312
Size: 108 1101 261 282 1148 865 298 3024
Fragments arranged by size:
3024 1148 1101 865 298 282 261 108
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3380 5750 882
Size: 617 1881 2370 2219
Fragments arranged by size:
2370 2219 1881 617
BsaAI yAC'GTr
Cuts at: 493 1204 2262 4878 493
Size: 711 1058 2616 2702
Fragments arranged by size:
2702 2616 1058 711
BsaBI GATnn'nnATC
Cuts at: 3143 7081 3143
Size: 3938 3149
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4344 4894 5276 5911
Size: 53 83 186 936 2811 550 382 635
Cuts at: 5911 275
Size: 1451
Fragments arranged by size:
2811 1451 936 635 550 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5520 5918 6192 6339 6750 5520
Size: 398 274 147 411 5857
Fragments arranged by size:
5857 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2036 2066 2186 2216 2246
Size: 30 165 30 633 30 120 30 30
Cuts at: 2246 2276 2408 2438 2900 2930 2969 2999 3086
Size: 30 132 30 462 30 39 30 87
Cuts at: 3086 3116 1178
Size: 30 5149
Fragments arranged by size:
5149 633 462 165 132 120 87 39
30 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2194 2212 2221 2267 2312 2612 2654
Size: 837 171 18 9 46 45 300 42
Cuts at: 2654 2657 3146 3248 3536 3941 4109 4229 1186
Size: 3 489 102 288 405 168 120 4044
Fragments arranged by size:
4044 837 489 405 300 288 171 168
120 102 46 45 42 18 9 3
BseYI C'CCAG_C
Cuts at: 2705 6290 6786 2705
Size: 3585 496 3006
Fragments arranged by size:
3585 3006 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2410 3301 3834 1163
Size: 573 674 891 533 4416
Fragments arranged by size:
4416 891 674 573 533
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3294 3637 4310 4537 5298 6326
Size: 275 186 1293 343 673 227 761 1028
Cuts at: 6326 1540
Size: 2301
Fragments arranged by size:
2301 1293 1028 761 673 343 275 227
186
BsiHKAI G_wGCw'C
Cuts at: 2697 2721 3320 3600 4080 5148 5233 6304 2697
Size: 24 599 280 480 1068 85 1071 3480
Fragments arranged by size:
3480 1071 1068 599 480 280 85 24
BsmI GAATG_Cn'
Cuts at: 4598 4691 4598
Size: 93 6994
BsmBI CGTCTCn'nnnn_
Cuts at: 3410 3758 3410
Size: 348 6739
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2027 2697 2721 2727 3320 3436
Size: 399 225 12 670 24 6 593 116
Cuts at: 3436 3600 4080 4130 4406 5148 5233 6304 1391
Size: 164 480 50 276 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 670 593 480 399 276
225 164 116 85 50 24 12 6
BspEI T'CCGG_A
Cuts at: 6750 6750
Size: 7087
BspHI T'CATG_A
Cuts at: 2550 4976 6706 2550
Size: 2426 1730 2931
Fragments arranged by size:
2931 2426 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2072 2932 3309 4442 844
Size: 1228 860 377 1133 3489
Fragments arranged by size:
3489 1228 1133 860 377
BsrBI CCG'CTC
Cuts at: 1394 1823 4306 4974 1394
Size: 429 2483 668 3507
Fragments arranged by size:
3507 2483 668 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3993 5582 5756 65
Size: 1183 2745 1589 174 1396
Fragments arranged by size:
2745 1589 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2139 2682 3168 4309 5731 5918 1700
Size: 110 329 543 486 1141 1422 187 2869
Fragments arranged by size:
2869 1422 1141 543 486 329 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7087
BssHII G'CGCG_C
Cuts at: 1925 3814 4283 1925
Size: 1889 469 4729
Fragments arranged by size:
4729 1889 469
BssSI C'ACGA_G
Cuts at: 2498 5147 6159 2498
Size: 2649 1012 3426
Fragments arranged by size:
3426 2649 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7087
BstBI TT'CG_AA
Cuts at: 5891 5891
Size: 7087
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7087
BstYI r'GATC_y
Cuts at: 1972 2265 4008 4417 4784 5169 5186 6638 6724
Size: 293 1743 409 367 385 17 1452 86
Cuts at: 6724 6736 7082 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1743 1452 409 385 367 346 293
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2637 2637
Size: 7087
BtgI C'CryG_G
Cuts at: 513 1065 2004 2397 3628 3653 513
Size: 552 939 393 1231 25 3947
Fragments arranged by size:
3947 1231 939 552 393 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2461 4044 4293 487
Size: 683 56 1235 1583 249 3281
Fragments arranged by size:
3281 1583 1235 683 249 56
BtsI GCAGTG_nn'
Cuts at: 3284 4603 5397 5417 3284
Size: 1319 794 20 4954
Fragments arranged by size:
4954 1319 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7087
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7052
DraI TTT'AAA
Cuts at: 4391 4391
Size: 7087
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2408 3041 6094 809
Size: 507 1092 633 3053 1802
Fragments arranged by size:
3053 1802 1092 633 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2712 3291
Size: 54 1425 50 275 18 288 594 579
Cuts at: 3291 3634 3723 3975 4307 4311 4356 4534 5423
Size: 343 89 252 332 4 45 178 889
Cuts at: 5423 7052 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 594 579 343 332 288
275 252 178 89 54 50 45 43
18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3291 3634 4307 4534 1537
Size: 275 1479 343 673 227 4090
Fragments arranged by size:
4090 1479 673 343 275 227
EarI CTCTTCn'nnn_
Cuts at: 1353 2124 2546 2702 3080 3110 5017 1353
Size: 771 422 156 378 30 1907 3423
Fragments arranged by size:
3423 1907 771 422 378 156 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2206 2969 3245 3824 3971 4283
Size: 1064 138 617 763 276 579 147 312
Cuts at: 4283 5674 6048 6194 387
Size: 1391 374 146 1280
Fragments arranged by size:
1391 1280 1064 763 617 579 374 312
276 147 146 138
EcoICRI GAG'CTC
Cuts at: 2695 2719 3598 4078 2695
Size: 24 879 480 5704
Fragments arranged by size:
5704 879 480 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2168 2270 2927 3113 3404 3416
Size: 393 497 39 102 657 186 291 12
Cuts at: 3416 3548 4025 4121 4142 4322 5150 5747 6534
Size: 132 477 96 21 180 828 597 787
Cuts at: 6534 6802 1239
Size: 268 1524
Fragments arranged by size:
1524 828 787 657 597 497 477 393
291 268 186 180 132 102 96 39
21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2252 2528 2939 3200 3305 3725 2252
Size: 276 411 261 105 420 5614
Fragments arranged by size:
5614 420 411 276 261 105
EcoO109I rG'GnC_Cy
Cuts at: 2047 2536 3298 3432 3433 3718 4126 4144 2047
Size: 489 762 134 1 285 408 18 4990
Fragments arranged by size:
4990 762 489 408 285 134 18 1
EcoRI G'AATT_C
Cuts at: 4396 4774 4396
Size: 378 6709
FseI GG_CCGG'CC
Cuts at: 4313 4313
Size: 7087
FspI TGC'GCA
Cuts at: 5593 5593
Size: 7087
HaeII r_GCGC'y
Cuts at: 1678 2397 2604 3536 3824 4242 6234 1678
Size: 719 207 932 288 418 1992 2531
Fragments arranged by size:
2531 1992 932 719 418 288 207
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2036 2053 2068 2147
Size: 32 135 32 449 15 17 15 79
Cuts at: 2147 2179 2969 3001 3359 3391 3685 3717 4178
Size: 32 790 32 358 32 294 32 461
Cuts at: 4178 4210 5728 5760 5802 5834 1373
Size: 32 1518 32 42 32 2626
Fragments arranged by size:
2626 1518 790 461 449 358 294 135
79 42 32 32 32 32 32 32
32 32 32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 4431 4677 669
Size: 988 281 2493 246 3079
Fragments arranged by size:
3079 2493 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2797 748
Size: 2049 5038
HpaI GTT'AAC
Cuts at: 4677 4677
Size: 7087
KpnI G_GTAC'C
Cuts at: 4412 4412
Size: 7087
MfeI C'AATT_G
Cuts at: 4686 4686
Size: 7087
MluI A'CGCG_T
Cuts at: 6963 6963
Size: 7087
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2259 4188 6201 6385 1305
Size: 954 1929 2013 184 2007
Fragments arranged by size:
2013 2007 1929 954 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2120 10
Size: 54 1425 343 288 4977
Fragments arranged by size:
4977 1425 343 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2402 2630 2810 2891 5045 5404 5563
Size: 648 1236 228 180 81 2154 359 159
Cuts at: 5563 518
Size: 2042
Fragments arranged by size:
2154 2042 1236 648 359 228 180 159
81
MspA1I CmG'CkG
Cuts at: 1606 2968 3027 3184 3514 3589 3630 3639 4042
Size: 1362 59 157 330 75 41 9 403
Cuts at: 4042 4150 4503 5180 6328 6573 1606
Size: 108 353 677 1148 245 2120
Fragments arranged by size:
2120 1362 1148 677 403 353 330 245
157 108 75 59 41 9
NaeI GCC'GGC
Cuts at: 4311 4311
Size: 7087
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5596
Fragments arranged by size:
5596 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7087
NgoMIV G'CCGG_C
Cuts at: 4309 4309
Size: 7087
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7087
NotI GC'GGCC_GC
Cuts at: 3634 4534 3634
Size: 900 6187
NspI r_CATG'y
Cuts at: 1132 1229 2407 2752 4445 5990 1132
Size: 97 1178 345 1693 1545 2229
Fragments arranged by size:
2229 1693 1545 1178 345 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7087
PciI A'CATG_T
Cuts at: 5986 5986
Size: 7087
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2292 2376 3559 3721 4378 1108
Size: 1184 84 1183 162 657 3817
Fragments arranged by size:
3817 1184 1183 657 162 84
PfoI T'CCnGG_A
Cuts at: 2145 2145
Size: 7087
PmeI GTTT'AAAC
Cuts at: 4391 4391
Size: 7087
PmlI CAC'GTG
Cuts at: 2262 2262
Size: 7087
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2186 2218 2408 2440 2900 2932 5156 5188 6669
Size: 32 190 32 460 32 2224 32 1481
Cuts at: 6669 6701 2186
Size: 32 2572
Fragments arranged by size:
2572 2224 1481 460 190 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2536 3298 4144 2536
Size: 762 846 5479
Fragments arranged by size:
5479 846 762
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7087
PsiI TTA'TAA
Cuts at: 4657 4657
Size: 7087
PspOMI G'GGCC_C
Cuts at: 3432 4126 3432
Size: 694 6393
PstI C_TGCA'G
Cuts at: 830 2757 2943 3306 3519 3588 3594 3681 3828
Size: 1927 186 363 213 69 6 87 147
Cuts at: 3828 3840 3870 4251 4439 830
Size: 12 30 381 188 3478
Fragments arranged by size:
3478 1927 381 363 213 188 186 147
87 69 30 12 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7087
PvuII CAG'CTG
Cuts at: 2968 3027 3514 3589 4042 2968
Size: 59 487 75 453 6013
Fragments arranged by size:
6013 487 453 75 59
SacI G_AGCT'C
Cuts at: 2697 2721 3600 4080 2697
Size: 24 879 480 5704
Fragments arranged by size:
5704 879 480 24
SacII CC_GC'GG
Cuts at: 3631 3631
Size: 7087
SalI G'TCGA_C
Cuts at: 1936 4429 1936
Size: 2493 4594
SapI GCTCTTCn'nnn_
Cuts at: 2124 2702 3110 2124
Size: 578 408 6101
Fragments arranged by size:
6101 578 408
SbfI CC_TGCA'GG
Cuts at: 4251 4439 4251
Size: 188 6899
ScaI AGT'ACT
Cuts at: 2423 2993 5335 7047 2423
Size: 570 2342 1712 2463
Fragments arranged by size:
2463 2342 1712 570
SexAI A'CCwGG_T
Cuts at: 3057 3057
Size: 7087
SfcI C'TryA_G
Cuts at: 826 1045 2753 2939 3302 3332 3515 3584 3590
Size: 219 1708 186 363 30 183 69 6
Cuts at: 3590 3677 3824 3836 3866 4247 4366 4435 5570
Size: 87 147 12 30 381 119 69 1135
Cuts at: 5570 6251 6442 826
Size: 681 191 1471
Fragments arranged by size:
1708 1471 1135 681 381 363 219 191
186 183 147 119 87 69 69 30
30 12 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2054 3623 2054
Size: 1569 5518
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7087
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7087
SmaI CCC'GGG
Cuts at: 1872 2876 4414 1872
Size: 1004 1538 4545
Fragments arranged by size:
4545 1538 1004
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2327 2435 3536 3761 4079 5191
Size: 197 926 384 108 1101 225 318 1112
Cuts at: 5191 6092 6354 820
Size: 901 262 1553
Fragments arranged by size:
1553 1112 1101 926 901 384 318 262
225 197 108
SnaBI TAC'GTA
Cuts at: 493 4878 493
Size: 4385 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7087
SphI G_CATG'C
Cuts at: 1132 4445 1132
Size: 3313 3774
SspI AAT'ATT
Cuts at: 5 52 5011 5
Size: 47 4959 2081
Fragments arranged by size:
4959 2081 47
StuI AGG'CCT
Cuts at: 1517 2648 1517
Size: 1131 5956
StyI C'CwwG_G
Cuts at: 513 1065 2004 4314 4526 513
Size: 552 939 2310 212 3074
Fragments arranged by size:
3074 2310 939 552 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2795 3404 5114 5131 5284 5469 5914
Size: 1255 1215 609 1710 17 153 185 445
Cuts at: 5914 325
Size: 1498
Fragments arranged by size:
1710 1498 1255 1215 609 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 2745 2991
Size: 274 80 33 51 1443 444 324 246
Cuts at: 2991 3066 4065 5333 7045 96
Size: 75 999 1268 1712 138
Fragments arranged by size:
1712 1443 1268 999 444 324 274 246
138 80 75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2246 2278 2774 2806 7047 7079 2246
Size: 32 496 32 4241 32 2254
Fragments arranged by size:
4241 2254 496 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7087
XbaI T'CTAG_A
Cuts at: 4423 4423
Size: 7087
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7087
XmaI C'CCGG_G
Cuts at: 1870 2874 4412 1870
Size: 1004 1538 4545
Fragments arranged by size:
4545 1538 1004
XmnI GAAnn'nnTTC
Cuts at: 5216 5216
Size: 7087
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4895 276
Size: 53 83 186 936 3361 2468
Fragments arranged by size:
3361 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI
BstZ17I DraIII EcoRV FalI FspAI KasI NarI NruI
NsiI PacI PspXI PsrI RsrII SanDI SfoI SrfI
SwaI XcmI