Restriction map for
pFN21AE4223
(Circular) (Six-base) MAPSORT of: pFN21AE4223.seq Check: 2050 from: 1 to: 7033
pFN21AE4223 7033 bp
With 182 enzymes: SgfI *
March 28, 2012 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2248 2248
Size: 7033
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4843 278
Size: 53 83 186 936 3307 2468
Fragments arranged by size:
3307 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4376 1937
Size: 2439 4594
Acc65I G'GTAC_C
Cuts at: 2023 4354 2023
Size: 2331 4702
AclI AA'CG_TT
Cuts at: 1563 2477 5160 5533 1563
Size: 914 2683 373 3063
Fragments arranged by size:
3063 2683 914 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2925 5096 6480 6748 1632
Size: 1293 2171 1384 268 1917
Fragments arranged by size:
2171 1917 1384 1293 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6836
AflIII A'CryG_T
Cuts at: 1203 5932 6909 1203
Size: 4729 977 1327
Fragments arranged by size:
4729 1327 977
AgeI A'CCGG_T
Cuts at: 5864 5864
Size: 7033
AhdI GACnn_n'nnGTC
Cuts at: 5762 5762
Size: 7033
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3921 1184
Size: 2737 4296
AlwNI CAG_nnn'CTG
Cuts at: 4776 6348 4776
Size: 1572 5461
ApaLI G'TGCA_C
Cuts at: 5090 6246 5090
Size: 1156 5877
ApoI r'AATT_y
Cuts at: 1493 2169 2567 2909 3417 3506 3522 3773 3819
Size: 676 398 342 508 89 16 251 46
Cuts at: 3819 3978 4302 4309 4342 4568 4720 6829 1493
Size: 159 324 7 33 226 152 2109 1697
Fragments arranged by size:
2109 1697 676 508 398 342 324 251
226 159 152 89 46 33 16 7
AseI AT'TA_AT
Cuts at: 160 1032 2559 5587 160
Size: 872 1527 3028 1606
Fragments arranged by size:
3028 1606 1527 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
AvaI C'yCGr_G
Cuts at: 1870 1943 4358 1870
Size: 73 2415 4545
Fragments arranged by size:
4545 2415 73
BamHI G'GATC_C
Cuts at: 4363 4730 4363
Size: 367 6666
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2023 2126 4354 5809 618
Size: 325 217 627 236 103 2228 1455 1842
Fragments arranged by size:
2228 1842 1455 627 325 236 217 103
BanII G_rGCy'C
Cuts at: 1391 3605 4352 1391
Size: 2214 747 4072
Fragments arranged by size:
4072 2214 747
BbeI G_GCGC'C
Cuts at: 2130 2130
Size: 7033
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2553 2627 3229 928
Size: 971 654 74 602 4732
Fragments arranged by size:
4732 971 654 602 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5224 5258 1903
Size: 34 3287 34 3678
Fragments arranged by size:
3678 3287 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4925 6135 4925
Size: 1210 5823
BclI T'GATC_A
Cuts at: 1548 3622 4293 1548
Size: 2074 671 4288
Fragments arranged by size:
4288 2074 671
BfrBI ATG'CAT
Cuts at: 2333 4012 2333
Size: 1679 5354
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5644 136
Size: 107 122 71 631 605 3972 1525
Fragments arranged by size:
3972 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7028 7028
Size: 7033
BlpI GC'TnA_GC
Cuts at: 4450 4450
Size: 7033
Bme1580I G_kGCm'C
Cuts at: 1790 5094 6250 1790
Size: 3304 1156 2573
Fragments arranged by size:
3304 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7033
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2351 4764 5722
Size: 416 53 83 399 24 934 2413 958
Cuts at: 5722 442
Size: 1753
Fragments arranged by size:
2413 1753 958 934 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7033
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4047 4047
Size: 7033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5693 1239
Size: 4454 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2592 4349 5158 6023 6321 2592
Size: 1757 809 865 298 3304
Fragments arranged by size:
3304 1757 865 809 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3223 5696 882
Size: 617 1724 2473 2219
Fragments arranged by size:
2473 2219 1724 617
BsaAI yAC'GTr
Cuts at: 493 1204 2742 4824 493
Size: 711 1538 2082 2702
Fragments arranged by size:
2702 2082 1538 711
BsaBI GATnn'nnATC
Cuts at: 2466 3185 7027 2466
Size: 719 3842 2472
Fragments arranged by size:
3842 2472 719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2127 4840 5222 5857
Size: 53 83 186 936 594 2713 382 635
Cuts at: 5857 275
Size: 1451
Fragments arranged by size:
2713 1451 936 635 594 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5466 5864 6138 6285 6696 5466
Size: 398 274 147 411 5803
Fragments arranged by size:
5803 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6808
Fragments arranged by size:
6808 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 2169 2595 1186
Size: 898 85 426 5624
Fragments arranged by size:
5624 898 426 85
BseYI C'CCAG_C
Cuts at: 6236 6732 6236
Size: 496 6537
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2226 1163
Size: 573 490 5970
Fragments arranged by size:
5970 573 490
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 2139 4483 5244 6272 1540
Size: 275 186 60 78 2344 761 1028 2301
Fragments arranged by size:
2344 2301 1028 761 275 186 78 60
BsiHKAI G_wGCw'C
Cuts at: 5094 5179 6250 5094
Size: 85 1071 5877
Fragments arranged by size:
5877 1071 85
BsmI GAATG_Cn'
Cuts at: 2581 4544 4637 2581
Size: 1963 93 4977
Fragments arranged by size:
4977 1963 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3605 4352 5094 5179 6250 1391
Size: 399 1815 747 742 85 1071 2174
Fragments arranged by size:
2174 1815 1071 747 742 399 85
BspEI T'CCGG_A
Cuts at: 6696 6696
Size: 7033
BspHI T'CATG_A
Cuts at: 4922 6652 4922
Size: 1730 5303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2248 4241 4388 844
Size: 1404 1993 147 3489
Fragments arranged by size:
3489 1993 1404 147
BsrBI CCG'CTC
Cuts at: 1394 1823 4920 1394
Size: 429 3097 3507
Fragments arranged by size:
3507 3097 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3655 3733 5528 5702 65
Size: 1183 2407 78 1795 174 1396
Fragments arranged by size:
2407 1795 1396 1183 174 78
BsrFI r'CCGG_y
Cuts at: 1700 1810 2129 5677 5864 1700
Size: 110 319 3548 187 2869
Fragments arranged by size:
3548 2869 319 187 110
BsrGI T'GTAC_A
Cuts at: 96 3853 96
Size: 3757 3276
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7033
BssSI C'ACGA_G
Cuts at: 5093 6105 5093
Size: 1012 6021
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4018 794
Size: 3224 3809
BstBI TT'CG_AA
Cuts at: 5837 5837
Size: 7033
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7033
BstXI CCAn_nnnn'nTGG
Cuts at: 2044 2044
Size: 7033
BstYI r'GATC_y
Cuts at: 1972 4276 4363 4730 5115 5132 6584 6670 6682
Size: 2304 87 367 385 17 1452 86 12
Cuts at: 6682 7028 1972
Size: 346 1977
Fragments arranged by size:
2304 1977 1452 385 367 346 87 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2067 2067
Size: 7033
BtgI C'CryG_G
Cuts at: 513 1065 2139 3657 3694 513
Size: 552 1074 1518 37 3852
Fragments arranged by size:
3852 1518 1074 552 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3172 487
Size: 683 56 1946 4348
Fragments arranged by size:
4348 1946 683 56
BtsI GCAGTG_nn'
Cuts at: 2523 3463 4549 5343 5363 2523
Size: 940 1086 794 20 4193
Fragments arranged by size:
4193 1086 940 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7033
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6998
DraI TTT'AAA
Cuts at: 2821 3487 3801 4183 4337 2821
Size: 666 314 382 154 5517
Fragments arranged by size:
5517 666 382 314 154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6040 809
Size: 507 4724 1802
Fragments arranged by size:
4724 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 4480 5369
Size: 54 1425 50 275 18 228 2422 889
Cuts at: 5369 6998 8
Size: 1629 43
Fragments arranged by size:
2422 1629 1425 889 275 228 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2058 4480 1537
Size: 275 246 2422 4090
Fragments arranged by size:
4090 2422 275 246
EarI CTCTTCn'nnn_
Cuts at: 1353 2831 3963 4963 1353
Size: 1478 1132 1000 3423
Fragments arranged by size:
3423 1478 1132 1000
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5620 5994 6140 387
Size: 1064 138 4031 374 146 1280
Fragments arranged by size:
4031 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2925 5096 5693 6480 6748 1239
Size: 393 1293 2171 597 787 268 1524
Fragments arranged by size:
2171 1524 1293 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 4158 4158
Size: 7033
EcoO109I rG'GnC_Cy
Cuts at: 2353 2353
Size: 7033
EcoRI G'AATT_C
Cuts at: 3506 3522 4342 4720 3506
Size: 16 820 378 5819
Fragments arranged by size:
5819 820 378 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2705 2705
Size: 7033
FspI TGC'GCA
Cuts at: 5539 5539
Size: 7033
HaeII r_GCGC'y
Cuts at: 1678 2130 6180 1678
Size: 452 4050 2531
Fragments arranged by size:
4050 2531 452
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 3200 3232 5674
Size: 32 135 32 749 32 847 32 2442
Cuts at: 5674 5706 5748 5780 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2442 847 749 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4377 4623 669
Size: 988 281 2439 246 3079
Fragments arranged by size:
3079 2439 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7033
HpaI GTT'AAC
Cuts at: 4623 4623
Size: 7033
KasI G'GCGC_C
Cuts at: 2126 2126
Size: 7033
KpnI G_GTAC'C
Cuts at: 2027 4358 2027
Size: 2331 4702
MfeI C'AATT_G
Cuts at: 2849 4632 2849
Size: 1783 5250
MluI A'CGCG_T
Cuts at: 6909 6909
Size: 7033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3499 6147 6331 1305
Size: 2194 2648 184 2007
Fragments arranged by size:
2648 2194 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5211
Fragments arranged by size:
5211 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4991 5350 5509 518
Size: 648 3825 359 159 2042
Fragments arranged by size:
3825 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2141 2198 4449 5126 6274 6519 1606
Size: 438 97 57 2251 677 1148 245 2120
Fragments arranged by size:
2251 2120 1148 677 438 245 97 57
NaeI GCC'GGC
Cuts at: 2131 2131
Size: 7033
NarI GG'CG_CC
Cuts at: 2127 2127
Size: 7033
NcoI C'CATG_G
Cuts at: 513 1065 3694 513
Size: 552 2629 3852
Fragments arranged by size:
3852 2629 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7033
NgoMIV G'CCGG_C
Cuts at: 2129 2129
Size: 7033
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7033
NotI GC'GGCC_GC
Cuts at: 2058 4480 2058
Size: 2422 4611
NsiI A_TGCA'T
Cuts at: 2335 4014 2335
Size: 1679 5354
NspI r_CATG'y
Cuts at: 1132 1229 3442 4016 4391 5936 1132
Size: 97 2213 574 375 1545 2229
Fragments arranged by size:
2229 2213 1545 574 375 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7033
PciI A'CATG_T
Cuts at: 5932 5932
Size: 7033
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3434 1108
Size: 2326 4707
PmeI GTTT'AAAC
Cuts at: 4337 4337
Size: 7033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5102 5134 6615 6647 5102
Size: 32 1481 32 5488
Fragments arranged by size:
5488 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3669 1613
Size: 2056 4977
PsiI TTA'TAA
Cuts at: 3546 4603 3546
Size: 1057 5976
PstI C_TGCA'G
Cuts at: 830 4023 4385 830
Size: 3193 362 3478
Fragments arranged by size:
3478 3193 362
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7033
PvuII CAG'CTG
Cuts at: 2198 2198
Size: 7033
SacII CC_GC'GG
Cuts at: 2142 2142
Size: 7033
SalI G'TCGA_C
Cuts at: 1936 4375 1936
Size: 2439 4594
SbfI CC_TGCA'GG
Cuts at: 4385 4385
Size: 7033
ScaI AGT'ACT
Cuts at: 4030 5281 6993 4030
Size: 1251 1712 4070
Fragments arranged by size:
4070 1712 1251
SfcI C'TryA_G
Cuts at: 826 1045 3132 4019 4134 4155 4381 5516 6197
Size: 219 2087 887 115 21 226 1135 681
Cuts at: 6197 6388 826
Size: 191 1471
Fragments arranged by size:
2087 1471 1135 887 681 226 219 191
115 21
SfoI GGC'GCC
Cuts at: 2128 2128
Size: 7033
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
SgrAI Cr'CCGG_yG
Cuts at: 1700 2129 1700
Size: 429 6604
SmaI CCC'GGG
Cuts at: 1872 4360 1872
Size: 2488 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2607 4328 5137 6038 6300 820
Size: 197 926 664 1721 809 901 262 1553
Fragments arranged by size:
1721 1553 926 901 809 664 262 197
SnaBI TAC'GTA
Cuts at: 493 2742 4824 493
Size: 2249 2082 2702
Fragments arranged by size:
2702 2249 2082
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7033
SphI G_CATG'C
Cuts at: 1132 4016 4391 1132
Size: 2884 375 3774
Fragments arranged by size:
3774 2884 375
SspI AAT'ATT
Cuts at: 5 52 4957 5
Size: 47 4905 2081
Fragments arranged by size:
4905 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7033
StyI C'CwwG_G
Cuts at: 513 1065 3558 3694 4472 513
Size: 552 2493 136 778 3074
Fragments arranged by size:
3074 2493 778 552 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3365 5060 5077 5230 5415 5860 325
Size: 1255 1785 1695 17 153 185 445 1498
Fragments arranged by size:
1785 1695 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2455 3853 4028
Size: 274 80 33 51 1443 478 1398 175
Cuts at: 4028 5279 6991 96
Size: 1251 1712 138
Fragments arranged by size:
1712 1443 1398 1251 478 274 175 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3086 3118 6993 7025 3086
Size: 32 3875 32 3094
Fragments arranged by size:
3875 3094 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7033
XbaI T'CTAG_A
Cuts at: 4369 4369
Size: 7033
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2044 2044
Size: 7033
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7033
XmaI C'CCGG_G
Cuts at: 1870 4358 1870
Size: 2488 4545
XmnI GAAnn'nnTTC
Cuts at: 2373 2840 2910 5162 2373
Size: 467 70 2252 4244
Fragments arranged by size:
4244 2252 467 70
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4841 276
Size: 53 83 186 936 3307 2468
Fragments arranged by size:
3307 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI