Restriction map for
pFN21AE4222
(Circular) (Six-base) MAPSORT of: pFN21AE4222.seq Check: 5877 from: 1 to: 7024
pFN21AE4222 7024 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2953 2953
Size: 7024
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4834 278
Size: 53 83 186 936 3298 2468
Fragments arranged by size:
3298 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2847 3799 4221 4367 1937
Size: 910 952 422 146 4594
Fragments arranged by size:
4594 952 910 422 146
Acc65I G'GTAC_C
Cuts at: 4345 4345
Size: 7024
AclI AA'CG_TT
Cuts at: 1563 5151 5524 1563
Size: 3588 373 3063
Fragments arranged by size:
3588 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2216 2541 2553 2560 2836 2897 3032 3126
Size: 584 325 12 7 276 61 135 94
Cuts at: 3126 4109 4148 5087 6471 6739 1632
Size: 983 39 939 1384 268 1917
Fragments arranged by size:
1917 1384 983 939 584 325 276 268
135 94 61 39 12 7
AfeI AGC'GCT
Cuts at: 2144 2144
Size: 7024
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6827
AflIII A'CryG_T
Cuts at: 1203 5923 6900 1203
Size: 4720 977 1327
Fragments arranged by size:
4720 1327 977
AgeI A'CCGG_T
Cuts at: 5855 5855
Size: 7024
AhdI GACnn_n'nnGTC
Cuts at: 3910 5753 3910
Size: 1843 5181
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7024
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7024
AlwNI CAG_nnn'CTG
Cuts at: 2342 2430 3149 3248 4767 6339 2342
Size: 88 719 99 1519 1572 3027
Fragments arranged by size:
3027 1572 1519 719 99 88
ApaI G_GGCC'C
Cuts at: 2242 2641 2242
Size: 399 6625
ApaLI G'TGCA_C
Cuts at: 5081 6237 5081
Size: 1156 5868
ApoI r'AATT_y
Cuts at: 1493 2748 3193 4093 4119 4333 4559 4711 6820
Size: 1255 445 900 26 214 226 152 2109
Cuts at: 6820 1493
Size: 1697
Fragments arranged by size:
2109 1697 1255 900 445 226 214 152
26
AseI AT'TA_AT
Cuts at: 160 1032 3416 3617 5578 160
Size: 872 2384 201 1961 1606
Fragments arranged by size:
2384 1961 1606 872 201
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7024
AvaI C'yCGr_G
Cuts at: 1870 1943 2252 2640 2657 4145 4349 1870
Size: 73 309 388 17 1488 204 4545
Fragments arranged by size:
4545 1488 388 309 204 73 17
BamHI G'GATC_C
Cuts at: 4354 4721 4354
Size: 367 6657
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2264 2351 4345 5800 618
Size: 325 217 627 477 87 1994 1455 1842
Fragments arranged by size:
1994 1842 1455 627 477 325 217 87
BanII G_rGCy'C
Cuts at: 1391 2242 2364 2641 4343 1391
Size: 851 122 277 1702 4072
Fragments arranged by size:
4072 1702 851 277 122
BbeI G_GCGC'C
Cuts at: 2268 2355 2268
Size: 87 6937
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6053
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5215 5249 1903
Size: 34 3278 34 3678
Fragments arranged by size:
3678 3278 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4916 6126 4916
Size: 1210 5814
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7024
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2645 2711 5635
Size: 107 122 71 631 605 973 66 2924
Cuts at: 5635 136
Size: 1525
Fragments arranged by size:
2924 1525 973 631 605 122 107 71
66
BglII A'GATC_T
Cuts at: 7019 7019
Size: 7024
BlpI GC'TnA_GC
Cuts at: 2920 4441 2920
Size: 1521 5503
Bme1580I G_kGCm'C
Cuts at: 1790 2242 2415 2641 5085 6241 1790
Size: 452 173 226 2444 1156 2573
Fragments arranged by size:
2573 2444 1156 452 226 173
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7024
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2724 4755 5713
Size: 416 53 83 399 24 1307 2031 958
Cuts at: 5713 442
Size: 1753
Fragments arranged by size:
2031 1753 1307 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7024
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2228 2338 3046 5684 1239
Size: 899 90 110 708 2638 2579
Fragments arranged by size:
2638 2579 899 708 110 90
Bpu10I CC'TnA_GC
Cuts at: 2393 3388 2393
Size: 995 6029
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3533 4056 5149 6014 6312 3533
Size: 523 1093 865 298 4245
Fragments arranged by size:
4245 1093 865 523 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2780 4305 5687 882
Size: 617 1281 1525 1382 2219
Fragments arranged by size:
2219 1525 1382 1281 617
BsaAI yAC'GTr
Cuts at: 493 1204 4815 493
Size: 711 3611 2702
Fragments arranged by size:
3611 2702 711
BsaBI GATnn'nnATC
Cuts at: 7018 7018
Size: 7024
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2265 2352 4831 5213
Size: 53 83 186 936 732 87 2479 382
Cuts at: 5213 5848 275
Size: 635 1451
Fragments arranged by size:
2479 1451 936 732 635 382 186 87
83 53
BsaWI w'CCGG_w
Cuts at: 3901 5457 5855 6129 6276 6687 3901
Size: 1556 398 274 147 411 4238
Fragments arranged by size:
4238 1556 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3577 3607 1178
Size: 30 165 30 2174 30 4595
Fragments arranged by size:
4595 2174 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 1186
Size: 1168 5856
BseYI C'CCAG_C
Cuts at: 2059 2145 2182 2311 6227 6723 2059
Size: 86 37 129 3916 496 2360
Fragments arranged by size:
3916 2360 496 129 86 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2654 1163
Size: 573 918 5533
Fragments arranged by size:
5533 918 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2130 2540 2649 4474 5235 6263
Size: 275 186 129 410 109 1825 761 1028
Cuts at: 6263 1540
Size: 2301
Fragments arranged by size:
2301 1825 1028 761 410 275 186 129
109
BsiHKAI G_wGCw'C
Cuts at: 3175 5085 5170 6241 3175
Size: 1910 85 1071 3958
Fragments arranged by size:
3958 1910 1071 85
BsmI GAATG_Cn'
Cuts at: 4535 4628 4535
Size: 93 6931
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2242 2364 2415 2641 3175 4343 5085
Size: 399 452 122 51 226 534 1168 742
Cuts at: 5085 5170 6241 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1168 1071 742 534 452 399 226
122 85 51
BspEI T'CCGG_A
Cuts at: 6687 6687
Size: 7024
BspHI T'CATG_A
Cuts at: 4054 4913 6643 4054
Size: 859 1730 4435
Fragments arranged by size:
4435 1730 859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2953 4192 4379 844
Size: 2109 1239 187 3489
Fragments arranged by size:
3489 2109 1239 187
BsrBI CCG'CTC
Cuts at: 1394 1823 2635 2759 4911 1394
Size: 429 812 124 2152 3507
Fragments arranged by size:
3507 2152 812 429 124
BsrDI GCAATG_nn'
Cuts at: 65 1248 5519 5693 65
Size: 1183 4271 174 1396
Fragments arranged by size:
4271 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2079 2295 2354 2401 2484 2648 5668
Size: 110 269 216 59 47 83 164 3020
Cuts at: 5668 5855 1700
Size: 187 2869
Fragments arranged by size:
3020 2869 269 216 187 164 110 83
59 47
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7024
BssHII G'CGCG_C
Cuts at: 1925 2122 2202 1925
Size: 197 80 6747
Fragments arranged by size:
6747 197 80
BssSI C'ACGA_G
Cuts at: 5084 6096 5084
Size: 1012 6012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7024
BstBI TT'CG_AA
Cuts at: 3790 4111 5828 3790
Size: 321 1717 4986
Fragments arranged by size:
4986 1717 321
BstEII G'GTnAC_C
Cuts at: 1184 2404 1184
Size: 1220 5804
BstYI r'GATC_y
Cuts at: 1972 4354 4721 5106 5123 6575 6661 6673 7019
Size: 2382 367 385 17 1452 86 12 346
Cuts at: 7019 1972
Size: 1977
Fragments arranged by size:
2382 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3800 3800
Size: 7024
Bsu36I CC'TnA_GG
Cuts at: 2841 2841
Size: 7024
BtgI C'CryG_G
Cuts at: 513 1065 2130 2164 2233 2305 2534 513
Size: 552 1065 34 69 72 229 5003
Fragments arranged by size:
5003 1065 552 229 72 69 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2545 2889 487
Size: 683 56 1319 344 4622
Fragments arranged by size:
4622 1319 683 344 56
BtsI GCAGTG_nn'
Cuts at: 3981 4540 5334 5354 3981
Size: 559 794 20 5651
Fragments arranged by size:
5651 794 559 20
ClaI AT'CG_AT
Cuts at: 1554 3420 1554
Size: 1866 5158
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6989
DraI TTT'AAA
Cuts at: 2788 2808 3228 3954 4328 2788
Size: 20 420 726 374 5484
Fragments arranged by size:
5484 726 420 374 20
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6031 809
Size: 507 4715 1802
Fragments arranged by size:
4715 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2107 2127 2188
Size: 54 1425 50 275 18 277 20 61
Cuts at: 2188 2244 2419 2537 2550 2604 2646 2697 4471
Size: 56 175 118 13 54 42 51 1774
Cuts at: 4471 5360 6989 8
Size: 889 1629 43
Fragments arranged by size:
1774 1629 1425 889 277 275 175 118
61 56 54 54 51 50 43 42
20 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 2127 2537 2646 4471 1537
Size: 275 315 410 109 1825 4090
Fragments arranged by size:
4090 1825 410 315 275 109
EarI CTCTTCn'nnn_
Cuts at: 1353 3075 4954 1353
Size: 1722 1879 3423
Fragments arranged by size:
3423 1879 1722
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2150 2291 5611 5985 6131 387
Size: 1064 138 561 141 3320 374 146 1280
Fragments arranged by size:
3320 1280 1064 561 374 146 141 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2216 2228 2338 2541 2553 2560
Size: 393 506 78 12 110 203 12 7
Cuts at: 2560 2836 2897 3032 3046 3126 4109 4148 5087
Size: 276 61 135 14 80 983 39 939
Cuts at: 5087 5684 6471 6739 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 983 939 787 597 506 393 276
268 203 135 110 80 78 61 39
14 12 12 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3153 3153
Size: 7024
EcoO109I rG'GnC_Cy
Cuts at: 2028 2238 2239 2692 2028
Size: 210 1 453 6360
Fragments arranged by size:
6360 453 210 1
EcoRI G'AATT_C
Cuts at: 4333 4711 4333
Size: 378 6646
FspI TGC'GCA
Cuts at: 5530 5530
Size: 7024
HaeII r_GCGC'y
Cuts at: 1678 2066 2146 2183 2228 2268 2343 2355 2683
Size: 388 80 37 45 40 75 12 328
Cuts at: 2683 2717 6171 1678
Size: 34 3454 2531
Fragments arranged by size:
3454 2531 388 328 80 75 45 40
37 34 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3074 3106 3629 3661 3896
Size: 32 135 32 1502 32 523 32 235
Cuts at: 3896 3928 4024 4056 5665 5697 5739 5771 1373
Size: 32 96 32 1609 32 42 32 2626
Fragments arranged by size:
2626 1609 1502 523 235 135 96 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4275 4368 4614 669
Size: 988 281 2337 93 246 3079
Fragments arranged by size:
3079 2337 988 281 246 93
HindIII A'AGCT_T
Cuts at: 748 3160 3559 748
Size: 2412 399 4213
Fragments arranged by size:
4213 2412 399
HpaI GTT'AAC
Cuts at: 4614 4614
Size: 7024
KasI G'GCGC_C
Cuts at: 2264 2351 2264
Size: 87 6937
KpnI G_GTAC'C
Cuts at: 4349 4349
Size: 7024
MfeI C'AATT_G
Cuts at: 4623 4623
Size: 7024
MluI A'CGCG_T
Cuts at: 6900 6900
Size: 7024
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 3588 3910 6138 6322 1305
Size: 813 1470 322 2228 184 2007
Fragments arranged by size:
2228 2007 1470 813 322 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2421 10
Size: 54 1425 343 589 4613
Fragments arranged by size:
4613 1425 589 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3037 4982 5341 5500 518
Size: 648 1871 1945 359 159 2042
Fragments arranged by size:
2042 1945 1871 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2106 2132 2166 2235 2439 2466 2536 3604
Size: 500 26 34 69 204 27 70 1068
Cuts at: 3604 4083 4237 4440 5117 6265 6510 1606
Size: 479 154 203 677 1148 245 2120
Fragments arranged by size:
2120 1148 1068 677 500 479 245 204
203 154 70 69 34 27 26
NaeI GCC'GGC
Cuts at: 2356 2650 2356
Size: 294 6730
NarI GG'CG_CC
Cuts at: 2265 2352 2265
Size: 87 6937
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6472
NdeI CA'TA_TG
Cuts at: 387 2767 387
Size: 2380 4644
NgoMIV G'CCGG_C
Cuts at: 2354 2648 2354
Size: 294 6730
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7024
NotI GC'GGCC_GC
Cuts at: 2127 2537 4471 2127
Size: 410 1934 4680
Fragments arranged by size:
4680 1934 410
NspI r_CATG'y
Cuts at: 1132 1229 3829 4382 5927 1132
Size: 97 2600 553 1545 2229
Fragments arranged by size:
2600 2229 1545 553 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7024
PciI A'CATG_T
Cuts at: 5923 5923
Size: 7024
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2116 2311 1108
Size: 1008 195 5821
Fragments arranged by size:
5821 1008 195
PfoI T'CCnGG_A
Cuts at: 3023 3023
Size: 7024
PmeI GTTT'AAAC
Cuts at: 4328 4328
Size: 7024
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5093 5125 6606 6638 5093
Size: 32 1481 32 5479
Fragments arranged by size:
5479 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2028 2028
Size: 7024
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7024
PsiI TTA'TAA
Cuts at: 4594 4594
Size: 7024
PspOMI G'GGCC_C
Cuts at: 2238 2637 2238
Size: 399 6625
PstI C_TGCA'G
Cuts at: 830 4082 4376 830
Size: 3252 294 3478
Fragments arranged by size:
3478 3252 294
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7024
PvuII CAG'CTG
Cuts at: 3604 4083 4237 3604
Size: 479 154 6391
Fragments arranged by size:
6391 479 154
SacII CC_GC'GG
Cuts at: 2133 2167 2236 2537 2133
Size: 34 69 301 6620
Fragments arranged by size:
6620 301 69 34
SalI G'TCGA_C
Cuts at: 1936 4366 1936
Size: 2430 4594
SbfI CC_TGCA'GG
Cuts at: 4376 4376
Size: 7024
ScaI AGT'ACT
Cuts at: 2868 5272 6984 2868
Size: 2404 1712 2908
Fragments arranged by size:
2908 2404 1712
SfcI C'TryA_G
Cuts at: 826 1045 4078 4218 4372 5507 6188 6379 826
Size: 219 3033 140 154 1135 681 191 1471
Fragments arranged by size:
3033 1471 1135 681 219 191 154 140
SfiI GGCCn_nnn'nGGCC
Cuts at: 2645 2645
Size: 7024
SfoI GGC'GCC
Cuts at: 2266 2353 2266
Size: 87 6937
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7024
SgrAI Cr'CCGG_yG
Cuts at: 1700 2354 1700
Size: 654 6370
SmaI CCC'GGG
Cuts at: 1872 2642 4351 1872
Size: 770 1709 4545
Fragments arranged by size:
4545 1709 770
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3512 4071 5128 6029 6291 820
Size: 197 926 1569 559 1057 901 262 1553
Fragments arranged by size:
1569 1553 1057 926 901 559 262 197
SnaBI TAC'GTA
Cuts at: 493 4815 493
Size: 4322 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7024
SphI G_CATG'C
Cuts at: 1132 4382 1132
Size: 3250 3774
SrfI GCCC'GGGC
Cuts at: 2642 2642
Size: 7024
SspI AAT'ATT
Cuts at: 5 52 3714 4948 5
Size: 47 3662 1234 2081
Fragments arranged by size:
3662 2081 1234 47
StuI AGG'CCT
Cuts at: 1517 3009 1517
Size: 1492 5532
StyI C'CwwG_G
Cuts at: 513 1065 4463 513
Size: 552 3398 3074
Fragments arranged by size:
3398 3074 552
SwaI ATTT'AAAT
Cuts at: 2808 2808
Size: 7024
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5051 5068 5221 5406 5851 325
Size: 1255 3471 17 153 185 445 1498
Fragments arranged by size:
3471 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2866 2959 3041
Size: 274 80 33 51 1443 889 93 82
Cuts at: 3041 5270 6982 96
Size: 2229 1712 138
Fragments arranged by size:
2229 1712 1443 889 274 138 93 82
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3577 3609 3900 3932 6984 7016 3577
Size: 32 291 32 3052 32 3585
Fragments arranged by size:
3585 3052 291 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7024
XbaI T'CTAG_A
Cuts at: 4307 4360 4307
Size: 53 6971
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7024
XmaI C'CCGG_G
Cuts at: 1870 2640 4349 1870
Size: 770 1709 4545
Fragments arranged by size:
4545 1709 770
XmnI GAAnn'nnTTC
Cuts at: 3249 4161 5153 3249
Size: 912 992 5120
Fragments arranged by size:
5120 992 912
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4832 276
Size: 53 83 186 936 3298 2468
Fragments arranged by size:
3298 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI DraIII EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SanDI SapI SexAI XcmI