Restriction map for
pFN21AE4221
(Circular) (Six-base) MAPSORT of: pFN21AE4221.seq Check: 5504 from: 1 to: 7006
pFN21AE4221 7006 bp
With 182 enzymes: SgfI *
March 28, 2012 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3972 4816 278
Size: 53 83 186 936 2436 844 2468
Fragments arranged by size:
2468 2436 936 844 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2567 4349 1937
Size: 630 1782 4594
Fragments arranged by size:
4594 1782 630
Acc65I G'GTAC_C
Cuts at: 4327 4327
Size: 7006
AclI AA'CG_TT
Cuts at: 1563 5133 5506 1563
Size: 3570 373 3063
Fragments arranged by size:
3570 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2926 5069 6453 6721 1632
Size: 1294 2143 1384 268 1917
Fragments arranged by size:
2143 1917 1384 1294 268
AfeI AGC'GCT
Cuts at: 3876 3876
Size: 7006
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6809
AflIII A'CryG_T
Cuts at: 1203 3045 5905 6882 1203
Size: 1842 2860 977 1327
Fragments arranged by size:
2860 1842 1327 977
AgeI A'CCGG_T
Cuts at: 5837 5837
Size: 7006
AhdI GACnn_n'nnGTC
Cuts at: 2574 3037 5735 2574
Size: 463 2698 3845
Fragments arranged by size:
3845 2698 463
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3092 1184
Size: 1908 5098
AlwNI CAG_nnn'CTG
Cuts at: 2167 3784 3985 4749 6321 2167
Size: 1617 201 764 1572 2852
Fragments arranged by size:
2852 1617 1572 764 201
ApaI G_GGCC'C
Cuts at: 3904 4271 3904
Size: 367 6639
ApaLI G'TGCA_C
Cuts at: 2141 4177 5063 6219 2141
Size: 2036 886 1156 2928
Fragments arranged by size:
2928 2036 1156 886
ApoI r'AATT_y
Cuts at: 1493 4315 4541 4693 6802 1493
Size: 2822 226 152 2109 1697
Fragments arranged by size:
2822 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5560 160
Size: 872 4528 1606
Fragments arranged by size:
4528 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7006
AvaI C'yCGr_G
Cuts at: 1870 1943 2303 2337 2615 3167 3425 3705 3863
Size: 73 360 34 278 552 258 280 158
Cuts at: 3863 4331 1870
Size: 468 4545
Fragments arranged by size:
4545 552 468 360 280 278 258 158
73 34
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3719 3752 3719
Size: 33 6973
BamHI G'GATC_C
Cuts at: 3549 4098 4336 4703 3549
Size: 549 238 367 5852
Fragments arranged by size:
5852 549 367 238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2326 2519 3616 4327 5782
Size: 325 217 627 539 193 1097 711 1455
Cuts at: 5782 618
Size: 1842
Fragments arranged by size:
1842 1455 1097 711 627 539 325 217
193
BanII G_rGCy'C
Cuts at: 1391 2312 2360 3904 4047 4271 4325 1391
Size: 921 48 1544 143 224 54 4072
Fragments arranged by size:
4072 1544 921 224 143 54 48
BbeI G_GCGC'C
Cuts at: 3620 3620
Size: 7006
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3525 928
Size: 971 1626 4409
Fragments arranged by size:
4409 1626 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2374 2408 4051 4085 5197 5231 1903
Size: 34 437 34 1643 34 1112 34 3678
Fragments arranged by size:
3678 1643 1112 437 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4898 6108 4898
Size: 1210 5796
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7006
BfrBI ATG'CAT
Cuts at: 3117 3117
Size: 7006
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2155 2340 3899
Size: 107 122 71 631 605 483 185 1559
Cuts at: 3899 3950 5617 136
Size: 51 1667 1525
Fragments arranged by size:
1667 1559 1525 631 605 483 185 122
107 71 51
BglII A'GATC_T
Cuts at: 7001 7001
Size: 7006
BlpI GC'TnA_GC
Cuts at: 4423 4423
Size: 7006
Bme1580I G_kGCm'C
Cuts at: 1790 2145 2524 3904 4181 4223 4271 5067 6223
Size: 355 379 1380 277 42 48 796 1156
Cuts at: 6223 1790
Size: 2573
Fragments arranged by size:
2573 1380 1156 796 379 355 277 48
42
BmgBI CAC'GTC
Cuts at: 1318 3046 3345 3478 1318
Size: 1728 299 133 4846
Fragments arranged by size:
4846 1728 299 133
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4737 5695 442
Size: 416 53 83 399 24 3320 958 1753
Fragments arranged by size:
3320 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7006
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2249 2653 2249
Size: 404 6602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2900 5666 1239
Size: 1661 2766 2579
Fragments arranged by size:
2766 2579 1661
Bpu10I CC'TnA_GC
Cuts at: 2359 2359
Size: 7006
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5131 5996 6294 5131
Size: 865 298 5843
Fragments arranged by size:
5843 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3793 5669 882
Size: 617 2294 1876 2219
Fragments arranged by size:
2294 2219 1876 617
BsaAI yAC'GTr
Cuts at: 493 1204 3766 4797 493
Size: 711 2562 1031 2702
Fragments arranged by size:
2702 2562 1031 711
BsaBI GATnn'nnATC
Cuts at: 7000 7000
Size: 7006
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2802 3195 3617 3969
Size: 53 83 186 936 1269 393 422 352
Cuts at: 3969 4277 4813 5195 5830 275
Size: 308 536 382 635 1451
Fragments arranged by size:
1451 1269 936 635 536 422 393 382
352 308 186 83 53
BsaWI w'CCGG_w
Cuts at: 3483 4053 5439 5837 6111 6258 6669 3483
Size: 570 1386 398 274 147 411 3820
Fragments arranged by size:
3820 1386 570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2363 2393 2687 2717 2909
Size: 30 165 30 960 30 294 30 192
Cuts at: 2909 2939 3164 3194 3230 3260 4142 4172 1178
Size: 30 225 30 36 30 882 30 4012
Fragments arranged by size:
4012 960 882 294 225 192 165 36
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2294 2501 2609 2642 2695 3185 3254
Size: 840 268 207 108 33 53 490 69
Cuts at: 3254 3257 3365 3443 3559 4078 1186
Size: 3 108 78 116 519 4114
Fragments arranged by size:
4114 840 519 490 268 207 116 108
108 78 69 53 33 3
BseYI C'CCAG_C
Cuts at: 2167 2221 2523 2850 3226 3793 3897 6209 6705
Size: 54 302 327 376 567 104 2312 496
Cuts at: 6705 2167
Size: 2468
Fragments arranged by size:
2468 2312 567 496 376 327 302 104
54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2941 3052 3476 3992 4097 1163
Size: 573 1205 111 424 516 105 4072
Fragments arranged by size:
4072 1205 573 516 424 111 105
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4456 5217 6245 1540
Size: 275 186 2455 761 1028 2301
Fragments arranged by size:
2455 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 2178 2312 2360 3435 4047 4181 5067 5152
Size: 33 134 48 1075 612 134 886 85
Cuts at: 5152 6223 2145
Size: 1071 2928
Fragments arranged by size:
2928 1075 1071 886 612 134 134 85
48 33
BsmI GAATG_Cn'
Cuts at: 4517 4610 4517
Size: 93 6913
BsmBI CGTCTCn'nnnn_
Cuts at: 3152 3202 3152
Size: 50 6956
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2178 2312 2360 2524 3435 3904
Size: 399 355 33 134 48 164 911 469
Cuts at: 3904 4047 4181 4223 4271 4325 5067 5152 6223
Size: 143 134 42 48 54 742 85 1071
Cuts at: 6223 1391
Size: 2174
Fragments arranged by size:
2174 1071 911 742 469 399 355 164
143 134 134 85 54 48 48 42
33
BspEI T'CCGG_A
Cuts at: 3483 4053 6669 3483
Size: 570 2616 3820
Fragments arranged by size:
3820 2616 570
BspHI T'CATG_A
Cuts at: 4895 6625 4895
Size: 1730 5276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3085 3747 4361 844
Size: 2241 662 614 3489
Fragments arranged by size:
3489 2241 662 614
BsrBI CCG'CTC
Cuts at: 1394 1823 3355 4893 1394
Size: 429 1532 1538 3507
Fragments arranged by size:
3507 1538 1532 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5501 5675 65
Size: 1183 4253 174 1396
Fragments arranged by size:
4253 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2343 2804 4181 5650 5837 1700
Size: 110 533 461 1377 1469 187 2869
Fragments arranged by size:
2869 1469 1377 533 461 187 110
BsrGI T'GTAC_A
Cuts at: 96 2199 3180 3570 96
Size: 2103 981 390 3532
Fragments arranged by size:
3532 2103 981 390
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7006
BssSI C'ACGA_G
Cuts at: 3335 5066 6078 3335
Size: 1731 1012 4263
Fragments arranged by size:
4263 1731 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7006
BstBI TT'CG_AA
Cuts at: 5810 5810
Size: 7006
BstEII G'GTnAC_C
Cuts at: 1184 2186 1184
Size: 1002 6004
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 7006
BstYI r'GATC_y
Cuts at: 1972 2559 3396 3549 3882 4098 4336 4703 5088
Size: 587 837 153 333 216 238 367 385
Cuts at: 5088 5105 6557 6643 6655 7001 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 837 587 385 367 346 333
238 216 153 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4250 4250
Size: 7006
BtgI C'CryG_G
Cuts at: 513 1065 2609 2752 3019 3295 3990 4167 513
Size: 552 1544 143 267 276 695 177 3352
Fragments arranged by size:
3352 1544 695 552 276 267 177 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6267
Fragments arranged by size:
6267 683 56
BtsI GCAGTG_nn'
Cuts at: 3062 4522 5316 5336 3062
Size: 1460 794 20 4732
Fragments arranged by size:
4732 1460 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7006
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6971
DraI TTT'AAA
Cuts at: 4310 4310
Size: 7006
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3095 6013 809
Size: 507 1779 2918 1802
Fragments arranged by size:
2918 1802 1779 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2742 2965 3627
Size: 54 1425 50 275 18 912 223 662
Cuts at: 3627 3942 3951 3987 4023 4183 4453 5342 6971
Size: 315 9 36 36 160 270 889 1629
Cuts at: 6971 8
Size: 43
Fragments arranged by size:
1629 1425 912 889 662 315 275 270
223 160 54 50 43 36 36 18
9
EagI C'GGCC_G
Cuts at: 1537 1812 4453 1537
Size: 275 2641 4090
Fragments arranged by size:
4090 2641 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3824 4936 1353
Size: 2471 1112 3423
Fragments arranged by size:
3423 2471 1112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2921 3122 3190 4073 5593 5967
Size: 1064 138 1332 201 68 883 1520 374
Cuts at: 5967 6113 387
Size: 146 1280
Fragments arranged by size:
1520 1332 1280 1064 883 374 201 146
138 68
EcoICRI GAG'CTC
Cuts at: 2310 2358 4045 2310
Size: 48 1687 5271
Fragments arranged by size:
5271 1687 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2900 2926 5069 5666 6453 6721 1239
Size: 393 1268 26 2143 597 787 268 1524
Fragments arranged by size:
2143 1524 1268 787 597 393 268 26
EcoO109I rG'GnC_Cy
Cuts at: 2031 2088 2248 2284 3634 4267 2031
Size: 57 160 36 1350 633 4770
Fragments arranged by size:
4770 1350 633 160 57 36
EcoRI G'AATT_C
Cuts at: 4315 4693 4315
Size: 378 6628
FspI TGC'GCA
Cuts at: 5512 5512
Size: 7006
HaeII r_GCGC'y
Cuts at: 1678 2741 3365 3620 3878 6153 1678
Size: 1063 624 255 258 2275 2531
Fragments arranged by size:
2531 2275 1063 624 258 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2100 2132 2560 2592 2675
Size: 32 135 32 528 32 428 32 83
Cuts at: 2675 2687 2707 2719 3023 3055 3329 3361 4142
Size: 12 20 12 304 32 274 32 781
Cuts at: 4142 4174 5647 5679 5721 5753 1373
Size: 32 1473 32 42 32 2626
Fragments arranged by size:
2626 1473 781 528 428 304 274 135
83 42 32 32 32 32 32 32
32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2568 4350 4596 669
Size: 988 281 630 1782 246 3079
Fragments arranged by size:
3079 1782 988 630 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7006
HpaI GTT'AAC
Cuts at: 4596 4596
Size: 7006
KasI G'GCGC_C
Cuts at: 3616 3616
Size: 7006
KpnI G_GTAC'C
Cuts at: 4331 4331
Size: 7006
MfeI C'AATT_G
Cuts at: 4605 4605
Size: 7006
MluI A'CGCG_T
Cuts at: 6882 6882
Size: 7006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2823 3327 3585 6120 6304 1305
Size: 1518 504 258 2535 184 2007
Fragments arranged by size:
2535 2007 1518 504 258 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3944 3953 3989 4025 10
Size: 54 1425 343 2112 9 36 36 2991
Fragments arranged by size:
2991 2112 1425 343 54 36 36 9
MslI CAynn'nnrTG
Cuts at: 518 1166 2429 3074 3219 3938 4031 4964 5323
Size: 648 1263 645 145 719 93 933 359
Cuts at: 5323 5482 518
Size: 159 2042
Fragments arranged by size:
2042 1263 933 719 648 645 359 159
145 93
MspA1I CmG'CkG
Cuts at: 1606 2079 2085 2611 2754 2866 2964 2971 3297
Size: 473 6 526 143 112 98 7 326
Cuts at: 3297 3831 3985 4155 4169 4422 5099 6247 6492
Size: 534 154 170 14 253 677 1148 245
Cuts at: 6492 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 534 526 473 326 253
245 170 154 143 112 98 14 7
6
NaeI GCC'GGC
Cuts at: 2345 2806 2345
Size: 461 6545
NarI GG'CG_CC
Cuts at: 3617 3617
Size: 7006
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6454
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7006
NgoMIV G'CCGG_C
Cuts at: 2343 2804 2343
Size: 461 6545
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7006
NotI GC'GGCC_GC
Cuts at: 4453 4453
Size: 7006
NsiI A_TGCA'T
Cuts at: 3119 3119
Size: 7006
NspI r_CATG'y
Cuts at: 1132 1229 2461 2923 3117 3121 3181 4147 4364
Size: 97 1232 462 194 4 60 966 217
Cuts at: 4364 5909 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1232 966 462 217 194 97
60 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7006
PciI A'CATG_T
Cuts at: 5905 5905
Size: 7006
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3784 1108
Size: 2676 4330
PfoI T'CCnGG_A
Cuts at: 2100 2555 2817 3636 2100
Size: 455 262 819 5470
Fragments arranged by size:
5470 819 455 262
PmeI GTTT'AAAC
Cuts at: 4310 4310
Size: 7006
PmlI CAC'GTG
Cuts at: 3766 3766
Size: 7006
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5075 5107 6588 6620 5075
Size: 32 1481 32 5461
Fragments arranged by size:
5461 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2088 2248 3634 2088
Size: 160 1386 5460
Fragments arranged by size:
5460 1386 160
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7006
PsiI TTA'TAA
Cuts at: 4576 4576
Size: 7006
PspOMI G'GGCC_C
Cuts at: 3900 4267 3900
Size: 367 6639
PstI C_TGCA'G
Cuts at: 830 2163 2841 3375 3813 4109 4358 830
Size: 1333 678 534 438 296 249 3478
Fragments arranged by size:
3478 1333 678 534 438 296 249
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7006
PvuII CAG'CTG
Cuts at: 2971 3985 2971
Size: 1014 5992
SacI G_AGCT'C
Cuts at: 2312 2360 4047 2312
Size: 48 1687 5271
Fragments arranged by size:
5271 1687 48
SacII CC_GC'GG
Cuts at: 2612 2755 3298 4170 2612
Size: 143 543 872 5448
Fragments arranged by size:
5448 872 543 143
SalI G'TCGA_C
Cuts at: 1936 2566 4348 1936
Size: 630 1782 4594
Fragments arranged by size:
4594 1782 630
SanDI GG'GwC_CC
Cuts at: 2088 2248 2088
Size: 160 6846
SapI GCTCTTCn'nnn_
Cuts at: 3824 3824
Size: 7006
SbfI CC_TGCA'GG
Cuts at: 4358 4358
Size: 7006
ScaI AGT'ACT
Cuts at: 3389 5254 6966 3389
Size: 1865 1712 3429
Fragments arranged by size:
3429 1865 1712
SexAI A'CCwGG_T
Cuts at: 3609 3783 3609
Size: 174 6832
SfcI C'TryA_G
Cuts at: 826 1045 2159 2837 3371 3809 4105 4354 5489
Size: 219 1114 678 534 438 296 249 1135
Cuts at: 5489 6170 6361 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1114 681 678 534 438 296
249 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3899 3950 3899
Size: 51 6955
SfoI GGC'GCC
Cuts at: 3618 3618
Size: 7006
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7006
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7006
SmaI CCC'GGG
Cuts at: 1872 3707 4333 1872
Size: 1835 626 4545
Fragments arranged by size:
4545 1835 626
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5110 6011 6273 820
Size: 197 926 3167 901 262 1553
Fragments arranged by size:
3167 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4797 493
Size: 4304 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7006
SphI G_CATG'C
Cuts at: 1132 2461 3121 4364 1132
Size: 1329 660 1243 3774
Fragments arranged by size:
3774 1329 1243 660
SspI AAT'ATT
Cuts at: 5 52 4930 5
Size: 47 4878 2081
Fragments arranged by size:
4878 2081 47
StuI AGG'CCT
Cuts at: 1517 2954 3893 1517
Size: 1437 939 4630
Fragments arranged by size:
4630 1437 939
StyI C'CwwG_G
Cuts at: 513 1065 3595 4445 513
Size: 552 2530 850 3074
Fragments arranged by size:
3074 2530 850 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2196 5033 5050 5203 5388 5833 325
Size: 1255 616 2837 17 153 185 445 1498
Fragments arranged by size:
2837 1498 1255 616 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2199 2997 3180
Size: 274 80 33 51 1443 222 798 183
Cuts at: 3180 3246 3387 3570 5252 6964 96
Size: 66 141 183 1682 1712 138
Fragments arranged by size:
1712 1682 1443 798 274 222 183 183
141 138 80 66 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2363 2395 2909 2941 6966 6998 2363
Size: 32 514 32 4025 32 2371
Fragments arranged by size:
4025 2371 514 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3479 1316
Size: 2163 4843
XbaI T'CTAG_A
Cuts at: 4342 4342
Size: 7006
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2236 2968 4168 2236
Size: 732 1200 5074
Fragments arranged by size:
5074 1200 732
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7006
XmaI C'CCGG_G
Cuts at: 1870 3705 4331 1870
Size: 1835 626 4545
Fragments arranged by size:
4545 1835 626
XmnI GAAnn'nnTTC
Cuts at: 5135 5135
Size: 7006
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3970 4814 276
Size: 53 83 186 936 2436 844 2468
Fragments arranged by size:
2468 2436 936 844 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbvCI BsiWI BstZ17I DraIII
EcoNI EcoRV FalI FseI FspAI NruI PacI PspXI
PsrI RsrII SrfI SwaI