Restriction map for
pFN21AE4215
(Circular) (Six-base) MAPSORT of: pFN21AE4215.seq Check: 7720 from: 1 to: 6919
pFN21AE4215 6919 bp
With 182 enzymes: SgfI *
March 28, 2012 13:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4729 278
Size: 53 83 186 936 3193 2468
Fragments arranged by size:
3193 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4262 1937
Size: 2325 4594
Acc65I G'GTAC_C
Cuts at: 2307 3426 4240 2307
Size: 1119 814 4986
Fragments arranged by size:
4986 1119 814
AclI AA'CG_TT
Cuts at: 1563 5046 5419 1563
Size: 3483 373 3063
Fragments arranged by size:
3483 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2136 3042 3518 4039 4982 6366 6634 1632
Size: 504 906 476 521 943 1384 268 1917
Fragments arranged by size:
1917 1384 943 906 521 504 476 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6722
AflIII A'CryG_T
Cuts at: 1203 5818 6795 1203
Size: 4615 977 1327
Fragments arranged by size:
4615 1327 977
AgeI A'CCGG_T
Cuts at: 5750 5750
Size: 6919
AhdI GACnn_n'nnGTC
Cuts at: 2843 5648 2843
Size: 2805 4114
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6919
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6919
AlwNI CAG_nnn'CTG
Cuts at: 4662 6234 4662
Size: 1572 5347
ApaI G_GGCC'C
Cuts at: 2062 2062
Size: 6919
ApaLI G'TGCA_C
Cuts at: 4976 6132 4976
Size: 1156 5763
ApoI r'AATT_y
Cuts at: 1493 3473 4228 4454 4606 6715 1493
Size: 1980 755 226 152 2109 1697
Fragments arranged by size:
2109 1980 1697 755 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5473 160
Size: 872 4441 1606
Fragments arranged by size:
4441 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6919
AvaI C'yCGr_G
Cuts at: 1870 1943 3620 3740 4244 1870
Size: 73 1677 120 504 4545
Fragments arranged by size:
4545 1677 504 120 73
BamHI G'GATC_C
Cuts at: 4249 4616 4249
Size: 367 6552
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2272 2307 2906 3426 4240
Size: 325 217 627 485 35 599 520 814
Cuts at: 4240 5695 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 814 627 599 520 485 325
217 35
BanII G_rGCy'C
Cuts at: 1391 2062 2388 3110 3201 3420 3582 4238 1391
Size: 671 326 722 91 219 162 656 4072
Fragments arranged by size:
4072 722 671 656 326 219 162 91
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2009 2448 2858 4016 928
Size: 971 110 439 410 1158 3831
Fragments arranged by size:
3831 1158 971 439 410 110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4087 4121 4162 4196 5110 5144 1903
Size: 34 2150 34 41 34 914 34 3678
Fragments arranged by size:
3678 2150 914 41 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2981 3291 4811 6021 2981
Size: 310 1520 1210 3879
Fragments arranged by size:
3879 1520 1210 310
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6919
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5530 136
Size: 107 122 71 631 605 3858 1525
Fragments arranged by size:
3858 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6914 6914
Size: 6919
BlpI GC'TnA_GC
Cuts at: 3195 3200 3605 4336 3195
Size: 5 405 731 5778
Fragments arranged by size:
5778 731 405 5
Bme1580I G_kGCm'C
Cuts at: 1790 2062 2277 4980 6136 1790
Size: 272 215 2703 1156 2573
Fragments arranged by size:
2703 2573 1156 272 215
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6919
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2253 3122 4138
Size: 416 53 83 399 24 836 869 1016
Cuts at: 4138 4650 5608 442
Size: 512 958 1753
Fragments arranged by size:
1753 1016 958 869 836 512 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6919
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2818 3094 3120 3792 5579 1239
Size: 1579 276 26 672 1787 2579
Fragments arranged by size:
2579 1787 1579 672 276 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5044 5909 6207 5044
Size: 865 298 5756
Fragments arranged by size:
5756 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2311 3058 3743 5582 882
Size: 617 812 747 685 1839 2219
Fragments arranged by size:
2219 1839 812 747 685 617
BsaAI yAC'GTr
Cuts at: 493 1204 4710 493
Size: 711 3506 2702
Fragments arranged by size:
3506 2702 711
BsaBI GATnn'nnATC
Cuts at: 2969 6913 2969
Size: 3944 2975
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4726 5108 5743 275
Size: 53 83 186 936 3193 382 635 1451
Fragments arranged by size:
3193 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2346 3228 5352 5750 6024 6171 6582 2346
Size: 882 2124 398 274 147 411 2683
Fragments arranged by size:
2683 2124 882 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6694
Fragments arranged by size:
6694 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2682 3046 1186
Size: 1496 364 5059
Fragments arranged by size:
5059 1496 364
BseYI C'CCAG_C
Cuts at: 2055 2201 3044 3828 3876 6122 6618 2055
Size: 146 843 784 48 2246 496 2356
Fragments arranged by size:
2356 2246 843 784 496 146 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6346
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4369 5130 6158 1540
Size: 275 186 2368 761 1028 2301
Fragments arranged by size:
2368 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2945 3110 3201 3236 3582 4980 5065 6136 2945
Size: 165 91 35 346 1398 85 1071 3728
Fragments arranged by size:
3728 1398 1071 346 165 91 85 35
BsmI GAATG_Cn'
Cuts at: 2588 3787 4430 4523 2588
Size: 1199 643 93 4984
Fragments arranged by size:
4984 1199 643 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3725 4189 3725
Size: 464 6455
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2277 2388 2945 3110 3201 3236
Size: 399 272 215 111 557 165 91 35
Cuts at: 3236 3420 3582 4238 4980 5065 6136 1391
Size: 184 162 656 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 656 557 399 272 215
184 165 162 111 91 85 35
BspEI T'CCGG_A
Cuts at: 3228 6582 3228
Size: 3354 3565
BspHI T'CATG_A
Cuts at: 4808 6538 4808
Size: 1730 5189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3854 4274 844
Size: 3010 420 3489
Fragments arranged by size:
3489 3010 420
BsrBI CCG'CTC
Cuts at: 1394 1823 4806 1394
Size: 429 2983 3507
Fragments arranged by size:
3507 2983 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2148 2511 2596 3670 5414 5588 65
Size: 1183 900 363 85 1074 1744 174 1396
Fragments arranged by size:
1744 1396 1183 1074 900 363 174 85
BsrFI r'CCGG_y
Cuts at: 1700 1810 4154 5563 5750 1700
Size: 110 2344 1409 187 2869
Fragments arranged by size:
2869 2344 1409 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6919
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6919
BssSI C'ACGA_G
Cuts at: 4106 4979 5991 4106
Size: 873 1012 5034
Fragments arranged by size:
5034 1012 873
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6919
BstBI TT'CG_AA
Cuts at: 5723 5723
Size: 6919
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6919
BstXI CCAn_nnnn'nTGG
Cuts at: 2069 3397 3980 2069
Size: 1328 583 5008
Fragments arranged by size:
5008 1328 583
BstYI r'GATC_y
Cuts at: 1972 3689 4249 4616 5001 5018 6470 6556 6568
Size: 1717 560 367 385 17 1452 86 12
Cuts at: 6568 6914 1972
Size: 346 1977
Fragments arranged by size:
1977 1717 1452 560 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2068 2125 3685 513
Size: 552 939 64 57 1560 3747
Fragments arranged by size:
3747 1560 939 552 64 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6180
Fragments arranged by size:
6180 683 56
BtsI GCAGTG_nn'
Cuts at: 4435 5229 5249 4435
Size: 794 20 6105
Fragments arranged by size:
6105 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6919
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6884
DraI TTT'AAA
Cuts at: 4223 4223
Size: 6919
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5926 809
Size: 507 4610 1802
Fragments arranged by size:
4610 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2161 2177 2922
Size: 54 1425 50 275 18 331 16 745
Cuts at: 2922 3399 3996 4152 4156 4366 5255 6884 8
Size: 477 597 156 4 210 889 1629 43
Fragments arranged by size:
1629 1425 889 745 597 477 331 275
210 156 54 50 43 18 16 4
EagI C'GGCC_G
Cuts at: 1537 1812 4366 1537
Size: 275 2554 4090
Fragments arranged by size:
4090 2554 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3018 4207 4849 1353
Size: 1665 1189 642 3423
Fragments arranged by size:
3423 1665 1189 642
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4177 5506 5880 6026 387
Size: 1064 138 2588 1329 374 146 1280
Fragments arranged by size:
2588 1329 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3108 3199 3580 3108
Size: 91 381 6447
Fragments arranged by size:
6447 381 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2818 3042 3094 3120 3518 3792
Size: 393 504 682 224 52 26 398 274
Cuts at: 3792 4039 4982 5579 6366 6634 1239
Size: 247 943 597 787 268 1524
Fragments arranged by size:
1524 943 787 682 597 504 398 393
274 268 247 224 52 26
EcoO109I rG'GnC_Cy
Cuts at: 2205 2888 3493 3976 2205
Size: 683 605 483 5148
Fragments arranged by size:
5148 683 605 483
EcoRI G'AATT_C
Cuts at: 3473 4228 4606 3473
Size: 755 378 5786
Fragments arranged by size:
5786 755 378
EcoRV GAT'ATC
Cuts at: 3712 3712
Size: 6919
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3583 3583
Size: 6919
FseI GG_CCGG'CC
Cuts at: 4158 4158
Size: 6919
FspI TGC'GCA
Cuts at: 5425 5425
Size: 6919
HaeII r_GCGC'y
Cuts at: 1678 6066 1678
Size: 4388 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 2829 2861 3017
Size: 32 135 32 452 32 773 32 156
Cuts at: 3017 3049 3097 3129 4190 4222 5560 5592 5634
Size: 32 48 32 1061 32 1338 32 42
Cuts at: 5634 5666 1373
Size: 32 2626
Fragments arranged by size:
2626 1338 1061 773 452 156 135 48
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2578 3088 4263 4509 669
Size: 988 281 640 510 1175 246 3079
Fragments arranged by size:
3079 1175 988 640 510 281 246
HindIII A'AGCT_T
Cuts at: 748 3660 748
Size: 2912 4007
HpaI GTT'AAC
Cuts at: 3088 4509 3088
Size: 1421 5498
KpnI G_GTAC'C
Cuts at: 2311 3430 4244 2311
Size: 1119 814 4986
Fragments arranged by size:
4986 1119 814
MfeI C'AATT_G
Cuts at: 2841 4518 2841
Size: 1677 5242
MluI A'CGCG_T
Cuts at: 6795 6795
Size: 6919
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3306 4188 6033 6217 1305
Size: 2001 882 1845 184 2007
Fragments arranged by size:
2007 2001 1845 882 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2163 2179 2924 3401 3998
Size: 54 1425 343 331 16 745 477 597
Cuts at: 3998 10
Size: 2931
Fragments arranged by size:
2931 1425 745 597 477 343 331 54
16
MslI CAynn'nnrTG
Cuts at: 518 1166 2067 2930 3444 4877 5236 5395 518
Size: 648 901 863 514 1433 359 159 2042
Fragments arranged by size:
2042 1433 901 863 648 514 359 159
MspA1I CmG'CkG
Cuts at: 1606 2055 2992 3194 3522 3880 4217 4335 5012
Size: 449 937 202 328 358 337 118 677
Cuts at: 5012 6160 6405 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 937 677 449 358 337 328
245 202 118
NaeI GCC'GGC
Cuts at: 4156 4156
Size: 6919
NcoI C'CATG_G
Cuts at: 513 1065 2004 2068 2125 3685 513
Size: 552 939 64 57 1560 3747
Fragments arranged by size:
3747 1560 939 552 64 57
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6919
NgoMIV G'CCGG_C
Cuts at: 4154 4154
Size: 6919
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6919
NotI GC'GGCC_GC
Cuts at: 4366 4366
Size: 6919
NspI r_CATG'y
Cuts at: 1132 1229 4277 5822 1132
Size: 97 3048 1545 2229
Fragments arranged by size:
3048 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2312 1348
Size: 964 5955
PciI A'CATG_T
Cuts at: 5818 5818
Size: 6919
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2920 4150 1108
Size: 1812 1230 3877
Fragments arranged by size:
3877 1812 1230
PfoI T'CCnGG_A
Cuts at: 2226 3855 2226
Size: 1629 5290
PmeI GTTT'AAAC
Cuts at: 4223 4223
Size: 6919
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4988 5020 6501 6533 4988
Size: 32 1481 32 5374
Fragments arranged by size:
5374 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2205 2888 2205
Size: 683 6236
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6919
PsiI TTA'TAA
Cuts at: 4489 4489
Size: 6919
PspOMI G'GGCC_C
Cuts at: 2058 2058
Size: 6919
PstI C_TGCA'G
Cuts at: 830 2991 4076 4271 830
Size: 2161 1085 195 3478
Fragments arranged by size:
3478 2161 1085 195
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6919
PvuII CAG'CTG
Cuts at: 2055 2992 3194 2055
Size: 937 202 5780
Fragments arranged by size:
5780 937 202
SacI G_AGCT'C
Cuts at: 3110 3201 3582 3110
Size: 91 381 6447
Fragments arranged by size:
6447 381 91
SalI G'TCGA_C
Cuts at: 1936 4261 1936
Size: 2325 4594
SanDI GG'GwC_CC
Cuts at: 2205 2205
Size: 6919
SbfI CC_TGCA'GG
Cuts at: 4271 4271
Size: 6919
ScaI AGT'ACT
Cuts at: 3746 5167 6879 3746
Size: 1421 1712 3786
Fragments arranged by size:
3786 1712 1421
SfcI C'TryA_G
Cuts at: 826 1045 2152 2377 2987 3451 4072 4267 5402
Size: 219 1107 225 610 464 621 195 1135
Cuts at: 5402 6083 6274 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1107 681 621 610 464 225
219 195 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6919
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6919
SmaI CCC'GGG
Cuts at: 1872 4246 1872
Size: 2374 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3740 5023 5924 6186 820
Size: 197 926 1797 1283 901 262 1553
Fragments arranged by size:
1797 1553 1283 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4710 493
Size: 4217 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6919
SphI G_CATG'C
Cuts at: 1132 4277 1132
Size: 3145 3774
SspI AAT'ATT
Cuts at: 5 52 4843 5
Size: 47 4791 2081
Fragments arranged by size:
4791 2081 47
StuI AGG'CCT
Cuts at: 1517 2503 1517
Size: 986 5933
StyI C'CwwG_G
Cuts at: 513 1065 2004 2068 2125 3685 3979 4358 513
Size: 552 939 64 57 1560 294 379 3074
Fragments arranged by size:
3074 1560 939 552 379 294 64 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3842 4946 4963 5116 5301 5746 325
Size: 1255 2262 1104 17 153 185 445 1498
Fragments arranged by size:
2262 1498 1255 1104 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3744 5165 6877
Size: 274 80 33 51 1443 1767 1421 1712
Cuts at: 6877 96
Size: 138
Fragments arranged by size:
1767 1712 1443 1421 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2935 2967 6879 6911 2935
Size: 32 3912 32 2943
Fragments arranged by size:
3912 2943 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6919
XbaI T'CTAG_A
Cuts at: 4255 4255
Size: 6919
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2917 4166 2917
Size: 1249 5670
XhoI C'TCGA_G
Cuts at: 1943 3740 1943
Size: 1797 5122
XmaI C'CCGG_G
Cuts at: 1870 4244 1870
Size: 2374 4545
XmnI GAAnn'nnTTC
Cuts at: 5048 5048
Size: 6919
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4727 276
Size: 53 83 186 936 3193 2468
Fragments arranged by size:
3193 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI