Restriction map for
pFN21AE4212
(Circular) (Six-base) MAPSORT of: pFN21AE4212.seq Check: 6618 from: 1 to: 6793
pFN21AE4212 6793 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4603 278
Size: 53 83 186 936 3067 2468
Fragments arranged by size:
3067 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4136 1937
Size: 2199 4594
Acc65I G'GTAC_C
Cuts at: 4114 4114
Size: 6793
AclI AA'CG_TT
Cuts at: 1563 4920 5293 1563
Size: 3357 373 3063
Fragments arranged by size:
3357 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2688 4856 6240 6508 1632
Size: 1056 2168 1384 268 1917
Fragments arranged by size:
2168 1917 1384 1056 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6596
AflIII A'CryG_T
Cuts at: 1203 5692 6669 1203
Size: 4489 977 1327
Fragments arranged by size:
4489 1327 977
AgeI A'CCGG_T
Cuts at: 5624 5624
Size: 6793
AhdI GACnn_n'nnGTC
Cuts at: 2026 4058 5522 2026
Size: 2032 1464 3297
Fragments arranged by size:
3297 2032 1464
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6793
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6793
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2180 2212 2180
Size: 32 6761
AlwNI CAG_nnn'CTG
Cuts at: 4536 6108 4536
Size: 1572 5221
ApaI G_GGCC'C
Cuts at: 2290 2674 2290
Size: 384 6409
ApaLI G'TGCA_C
Cuts at: 4005 4850 6006 4005
Size: 845 1156 4792
Fragments arranged by size:
4792 1156 845
ApoI r'AATT_y
Cuts at: 1493 2834 2934 3091 3272 3540 4102 4328 4480
Size: 1341 100 157 181 268 562 226 152
Cuts at: 4480 6589 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1341 562 268 226 181 157
152 100
AseI AT'TA_AT
Cuts at: 160 1032 5347 160
Size: 872 4315 1606
Fragments arranged by size:
4315 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6793
AvaI C'yCGr_G
Cuts at: 1870 1943 2148 2230 2387 4118 1870
Size: 73 205 82 157 1731 4545
Fragments arranged by size:
4545 1731 205 157 82 73
BamHI G'GATC_C
Cuts at: 3796 4123 4490 3796
Size: 327 367 6099
Fragments arranged by size:
6099 367 327
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 2097 2371 2511 4114
Size: 325 217 627 304 6 274 140 1603
Cuts at: 4114 5569 618
Size: 1455 1842
Fragments arranged by size:
1842 1603 1455 627 325 304 274 217
140 6
BanII G_rGCy'C
Cuts at: 1391 2012 2290 2674 3322 4000 4016 4112 1391
Size: 621 278 384 648 678 16 96 4072
Fragments arranged by size:
4072 678 648 621 384 278 96 16
BbeI G_GCGC'C
Cuts at: 2095 2101 2095
Size: 6 6787
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2110 928
Size: 971 211 5611
Fragments arranged by size:
5611 971 211
BbvCI CC'TCA_GC
Cuts at: 2300 2879 2300
Size: 579 6214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4984 5018 1903
Size: 34 3047 34 3678
Fragments arranged by size:
3678 3047 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4685 5895 4685
Size: 1210 5583
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6793
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2082 2889 5404
Size: 107 122 71 631 605 410 807 2515
Cuts at: 5404 136
Size: 1525
Fragments arranged by size:
2515 1525 807 631 605 410 122 107
71
BglII A'GATC_T
Cuts at: 2859 6788 2859
Size: 3929 2864
BlpI GC'TnA_GC
Cuts at: 4210 4210
Size: 6793
Bme1580I G_kGCm'C
Cuts at: 1790 2290 2376 2516 2674 2954 3207 3464 4009
Size: 500 86 140 158 280 253 257 545
Cuts at: 4009 4024 4854 6010 1790
Size: 15 830 1156 2573
Fragments arranged by size:
2573 1156 830 545 500 280 257 253
158 140 86 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6793
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3131 4524 5482
Size: 416 53 83 399 24 1714 1393 958
Cuts at: 5482 442
Size: 1753
Fragments arranged by size:
1753 1714 1393 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6793
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2038 3970 2038
Size: 1932 4861
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 2952 3126 3334 3469 5453 1239
Size: 1133 580 174 208 135 1984 2579
Fragments arranged by size:
2579 1984 1133 580 208 174 135
Bpu10I CC'TnA_GC
Cuts at: 2280 2300 2879 2280
Size: 20 579 6194
Fragments arranged by size:
6194 579 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3308 4918 5783 6081 3308
Size: 1610 865 298 4020
Fragments arranged by size:
4020 1610 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2714 5456 882
Size: 617 1215 2742 2219
Fragments arranged by size:
2742 2219 1215 617
BsaAI yAC'GTr
Cuts at: 493 1204 4584 493
Size: 711 3380 2702
Fragments arranged by size:
3380 2702 711
BsaBI GATnn'nnATC
Cuts at: 6787 6787
Size: 6793
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2092 2098 4600 4982
Size: 53 83 186 936 559 6 2502 382
Cuts at: 4982 5617 275
Size: 635 1451
Fragments arranged by size:
2502 1451 936 635 559 382 186 83
53 6
BsaWI w'CCGG_w
Cuts at: 5226 5624 5898 6045 6456 5226
Size: 398 274 147 411 5563
Fragments arranged by size:
5563 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 2234 2264 1178
Size: 30 165 30 777 30 24 30 5707
Fragments arranged by size:
5707 777 165 30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 2255 2288 2477 3123 1186
Size: 1036 33 33 189 646 4856
Fragments arranged by size:
4856 1036 646 189 33 33
BseYI C'CCAG_C
Cuts at: 2947 3332 3870 5996 6492 2947
Size: 385 538 2126 496 3248
Fragments arranged by size:
3248 2126 538 496 385
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2413 2838 1163
Size: 573 677 425 5118
Fragments arranged by size:
5118 677 573 425
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 2089 2140 4243 5004 6032
Size: 275 186 57 31 51 2103 761 1028
Cuts at: 6032 1540
Size: 2301
Fragments arranged by size:
2301 2103 1028 761 275 186 57 51
31
BsiHKAI G_wGCw'C
Cuts at: 2968 3380 4009 4854 4939 6010 2968
Size: 412 629 845 85 1071 3751
Fragments arranged by size:
3751 1071 845 629 412 85
BsmI GAATG_Cn'
Cuts at: 3021 3477 3650 4304 4397 3021
Size: 456 173 654 93 5417
Fragments arranged by size:
5417 654 456 173 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2290 2376 2516 2674 2954 2968
Size: 399 222 278 86 140 158 280 14
Cuts at: 2968 3207 3322 3380 3464 4000 4009 4016 4024
Size: 239 115 58 84 536 9 7 8
Cuts at: 4024 4112 4854 4939 6010 1391
Size: 88 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 536 399 280 278 239
222 158 140 115 88 86 85 84
58 14 9 8 7
BspEI T'CCGG_A
Cuts at: 6456 6456
Size: 6793
BspHI T'CATG_A
Cuts at: 4682 6412 4682
Size: 1730 5063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2352 3045 3498 4148 844
Size: 1508 693 453 650 3489
Fragments arranged by size:
3489 1508 693 650 453
BsrBI CCG'CTC
Cuts at: 1394 1823 2284 2316 3963 4680 1394
Size: 429 461 32 1647 717 3507
Fragments arranged by size:
3507 1647 717 461 429 32
BsrDI GCAATG_nn'
Cuts at: 65 1248 2985 2994 3126 5288 5462 65
Size: 1183 1737 9 132 2162 174 1396
Fragments arranged by size:
2162 1737 1396 1183 174 132 9
BsrFI r'CCGG_y
Cuts at: 1700 1810 2088 2094 3605 5437 5624 1700
Size: 110 278 6 1511 1832 187 2869
Fragments arranged by size:
2869 1832 1511 278 187 110 6
BsrGI T'GTAC_A
Cuts at: 96 2894 96
Size: 2798 3995
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6793
BssSI C'ACGA_G
Cuts at: 2317 3594 4853 5865 2317
Size: 1277 1259 1012 3245
Fragments arranged by size:
3245 1277 1259 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6793
BstBI TT'CG_AA
Cuts at: 5597 5597
Size: 6793
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6793
BstXI CCAn_nnnn'nTGG
Cuts at: 3181 3181
Size: 6793
BstYI r'GATC_y
Cuts at: 1972 2859 3796 4123 4490 4875 4892 6344 6430
Size: 887 937 327 367 385 17 1452 86
Cuts at: 6430 6442 6788 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 937 887 385 367 346 327
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2134 2915 3009 513
Size: 552 939 130 781 94 4297
Fragments arranged by size:
4297 939 781 552 130 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2028 487
Size: 683 56 802 5252
Fragments arranged by size:
5252 802 683 56
BtsI GCAGTG_nn'
Cuts at: 2614 2773 2826 3793 3895 4309 5103 5123 2614
Size: 159 53 967 102 414 794 20 4284
Fragments arranged by size:
4284 967 794 414 159 102 53 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6793
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3282 3317 529
Size: 35 2718 35 4005
Fragments arranged by size:
4005 2718 35 35
DraI TTT'AAA
Cuts at: 4097 4097
Size: 6793
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5800 809
Size: 507 4484 1802
Fragments arranged by size:
4484 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2086 2137
Size: 54 1425 50 275 18 225 31 51
Cuts at: 2137 3435 3603 3840 4240 5129 6758 8
Size: 1298 168 237 400 889 1629 43
Fragments arranged by size:
1629 1425 1298 889 400 275 237 225
168 54 51 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 2055 2086 2137 4240 1537
Size: 275 243 31 51 2103 4090
Fragments arranged by size:
4090 2103 275 243 51 31
EarI CTCTTCn'nnn_
Cuts at: 1353 3786 4723 1353
Size: 2433 937 3423
Fragments arranged by size:
3423 2433 937
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2201 2231 2324 3795 5380 5754
Size: 1064 138 612 30 93 1471 1585 374
Cuts at: 5754 5900 387
Size: 146 1280
Fragments arranged by size:
1585 1471 1280 1064 612 374 146 138
93 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2372 2688 2952 3126 3334 3469 4856
Size: 393 740 316 264 174 208 135 1387
Cuts at: 4856 5453 6240 6508 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1387 787 740 597 393 316 268
264 208 174 135
EcoNI CCTnn'n_nnAGG
Cuts at: 4046 4046
Size: 6793
EcoO109I rG'GnC_Cy
Cuts at: 2287 2670 3419 2287
Size: 383 749 5661
Fragments arranged by size:
5661 749 383
EcoRI G'AATT_C
Cuts at: 2834 4102 4480 2834
Size: 1268 378 5147
Fragments arranged by size:
5147 1268 378
FspI TGC'GCA
Cuts at: 5299 5299
Size: 6793
HaeII r_GCGC'y
Cuts at: 1678 2095 2101 5940 1678
Size: 417 6 3839 2531
Fragments arranged by size:
3839 2531 417 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 2617 2649 2950
Size: 32 135 32 440 32 573 32 301
Cuts at: 2950 2982 3899 3923 3931 3955 4044 4076 5434
Size: 32 917 24 8 24 89 32 1358
Cuts at: 5434 5466 5508 5540 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1358 917 573 440 301 135 89
42 32 32 32 32 32 32 32
32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2663 4137 4383 669
Size: 988 281 725 1474 246 3079
Fragments arranged by size:
3079 1474 988 725 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6793
HpaI GTT'AAC
Cuts at: 4383 4383
Size: 6793
KasI G'GCGC_C
Cuts at: 2091 2097 2091
Size: 6 6787
KpnI G_GTAC'C
Cuts at: 4118 4118
Size: 6793
MfeI C'AATT_G
Cuts at: 4392 4392
Size: 6793
MluI A'CGCG_T
Cuts at: 6669 6669
Size: 6793
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3016 5907 6091 1305
Size: 1711 2891 184 2007
Fragments arranged by size:
2891 2007 1711 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3842 10
Size: 54 1425 343 2010 2961
Fragments arranged by size:
2961 2010 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3738 4751 5110 5269 518
Size: 648 2572 1013 359 159 2042
Fragments arranged by size:
2572 2042 1013 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2082 2136 2257 2304 2382 2508 2562 2947
Size: 476 54 121 47 78 126 54 385
Cuts at: 2947 3700 4209 4886 6034 6279 1606
Size: 753 509 677 1148 245 2120
Fragments arranged by size:
2120 1148 753 677 509 476 385 245
126 121 78 54 54 47
NaeI GCC'GGC
Cuts at: 2090 2096 2090
Size: 6 6787
NarI GG'CG_CC
Cuts at: 2092 2098 2092
Size: 6 6787
NcoI C'CATG_G
Cuts at: 513 1065 2004 2915 3009 513
Size: 552 939 911 94 4297
Fragments arranged by size:
4297 939 911 552 94
NdeI CA'TA_TG
Cuts at: 387 3187 4026 387
Size: 2800 839 3154
Fragments arranged by size:
3154 2800 839
NgoMIV G'CCGG_C
Cuts at: 2088 2094 2088
Size: 6 6787
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6793
NotI GC'GGCC_GC
Cuts at: 2055 2137 4240 2055
Size: 82 2103 4608
Fragments arranged by size:
4608 2103 82
NspI r_CATG'y
Cuts at: 1132 1229 2054 2649 4151 5696 1132
Size: 97 825 595 1502 1545 2229
Fragments arranged by size:
2229 1545 1502 825 595 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6793
PciI A'CATG_T
Cuts at: 5692 5692
Size: 6793
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4076 1108
Size: 2968 3825
PmeI GTTT'AAAC
Cuts at: 4097 4097
Size: 6793
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2180 2212 4862 4894 6375 6407 2180
Size: 32 2650 32 1481 32 2566
Fragments arranged by size:
2650 2566 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3419 3419
Size: 6793
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6793
PsiI TTA'TAA
Cuts at: 4363 4363
Size: 6793
PspOMI G'GGCC_C
Cuts at: 2286 2670 2286
Size: 384 6409
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2450 2482 2450
Size: 32 6761
PstI C_TGCA'G
Cuts at: 830 3151 3809 3976 4145 830
Size: 2321 658 167 169 3478
Fragments arranged by size:
3478 2321 658 169 167
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6793
PvuII CAG'CTG
Cuts at: 2304 2382 2562 2947 3700 2304
Size: 78 180 385 753 5397
Fragments arranged by size:
5397 753 385 180 78
SacII CC_GC'GG
Cuts at: 2137 2137
Size: 6793
SalI G'TCGA_C
Cuts at: 1936 4135 1936
Size: 2199 4594
SbfI CC_TGCA'GG
Cuts at: 4145 4145
Size: 6793
ScaI AGT'ACT
Cuts at: 5041 6753 5041
Size: 1712 5081
SfcI C'TryA_G
Cuts at: 826 1045 3147 3393 3805 3972 4141 5276 5957
Size: 219 2102 246 412 167 169 1135 681
Cuts at: 5957 6148 826
Size: 191 1471
Fragments arranged by size:
2102 1471 1135 681 412 246 219 191
169 167
SfoI GGC'GCC
Cuts at: 2093 2099 2093
Size: 6 6787
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6793
SgrAI Cr'CCGG_yG
Cuts at: 1700 2094 1700
Size: 394 6399
SmaI CCC'GGG
Cuts at: 1872 2150 2232 4120 1872
Size: 278 82 1888 4545
Fragments arranged by size:
4545 1888 278 82
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3323 4897 5798 6060 820
Size: 197 926 1380 1574 901 262 1553
Fragments arranged by size:
1574 1553 1380 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4584 493
Size: 4091 2702
SpeI A'CTAG_T
Cuts at: 152 3365 3380 152
Size: 3213 15 3565
Fragments arranged by size:
3565 3213 15
SphI G_CATG'C
Cuts at: 1132 4151 1132
Size: 3019 3774
SrfI GCCC'GGGC
Cuts at: 2150 2232 2150
Size: 82 6711
SspI AAT'ATT
Cuts at: 5 52 4717 5
Size: 47 4665 2081
Fragments arranged by size:
4665 2081 47
StuI AGG'CCT
Cuts at: 1517 3360 1517
Size: 1843 4950
StyI C'CwwG_G
Cuts at: 513 1065 2004 2915 3009 3851 4000 4232 513
Size: 552 939 911 94 842 149 232 3074
Fragments arranged by size:
3074 939 911 842 552 232 149 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3112 4820 4837 4990 5175 5620 325
Size: 1255 1532 1708 17 153 185 445 1498
Fragments arranged by size:
1708 1532 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2894 5039 6751
Size: 274 80 33 51 1443 917 2145 1712
Cuts at: 6751 96
Size: 138
Fragments arranged by size:
2145 1712 1443 917 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2234 2266 3246 3278 6753 6785 2234
Size: 32 980 32 3475 32 2242
Fragments arranged by size:
3475 2242 980 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3118 1316
Size: 1802 4991
XbaI T'CTAG_A
Cuts at: 4129 4129
Size: 6793
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3007 3007
Size: 6793
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6793
XmaI C'CCGG_G
Cuts at: 1870 2148 2230 4118 1870
Size: 278 82 1888 4545
Fragments arranged by size:
4545 1888 278 82
XmnI GAAnn'nnTTC
Cuts at: 4922 4922
Size: 6793
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4601 276
Size: 53 83 186 936 3067 2468
Fragments arranged by size:
3067 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI RsrII SacI
SanDI SapI SexAI SfiI SwaI