Restriction map for
pFN21AE4210
(Circular) (Six-base) MAPSORT of: pFN21AE4210.seq Check: 3105 from: 1 to: 6895
pFN21AE4210 6895 bp
With 182 enzymes: SgfI *
March 28, 2012 13:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4705 278
Size: 53 83 186 936 3169 2468
Fragments arranged by size:
3169 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4032 4238 1937
Size: 2095 206 4594
Fragments arranged by size:
4594 2095 206
Acc65I G'GTAC_C
Cuts at: 4216 4216
Size: 6895
AclI AA'CG_TT
Cuts at: 1563 5022 5395 1563
Size: 3459 373 3063
Fragments arranged by size:
3459 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2630 4958 6342 6610 1632
Size: 464 534 2328 1384 268 1917
Fragments arranged by size:
2328 1917 1384 534 464 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6698
AflIII A'CryG_T
Cuts at: 1203 5794 6771 1203
Size: 4591 977 1327
Fragments arranged by size:
4591 1327 977
AgeI A'CCGG_T
Cuts at: 5726 5726
Size: 6895
AhdI GACnn_n'nnGTC
Cuts at: 5624 5624
Size: 6895
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6895
AlwNI CAG_nnn'CTG
Cuts at: 2095 2394 2821 4638 6210 2095
Size: 299 427 1817 1572 2780
Fragments arranged by size:
2780 1817 1572 427 299
ApaI G_GGCC'C
Cuts at: 3281 3443 3281
Size: 162 6733
ApaLI G'TGCA_C
Cuts at: 4952 6108 4952
Size: 1156 5739
ApoI r'AATT_y
Cuts at: 1493 2786 3284 4148 4204 4430 4582 6691 1493
Size: 1293 498 864 56 226 152 2109 1697
Fragments arranged by size:
2109 1697 1293 864 498 226 152 56
AseI AT'TA_AT
Cuts at: 160 1032 5449 160
Size: 872 4417 1606
Fragments arranged by size:
4417 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6895
AvaI C'yCGr_G
Cuts at: 1870 1943 2447 3694 4045 4132 4220 1870
Size: 73 504 1247 351 87 88 4545
Fragments arranged by size:
4545 1247 504 351 88 87 73
BamHI G'GATC_C
Cuts at: 2628 4225 4592 2628
Size: 1597 367 4931
Fragments arranged by size:
4931 1597 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2152 2366 4216 5671 618
Size: 325 217 627 365 214 1850 1455 1842
Fragments arranged by size:
1850 1842 1455 627 365 325 217 214
BanII G_rGCy'C
Cuts at: 1391 2858 3281 3408 3443 3702 3725 4061 4214
Size: 1467 423 127 35 259 23 336 153
Cuts at: 4214 1391
Size: 4072
Fragments arranged by size:
4072 1467 423 336 259 153 127 35
23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2618 928
Size: 971 719 5205
Fragments arranged by size:
5205 971 719
BbvCI CC'TCA_GC
Cuts at: 2420 3584 2420
Size: 1164 5731
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2884 2918 5086 5120 1903
Size: 34 947 34 2168 34 3678
Fragments arranged by size:
3678 2168 947 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4787 5997 4787
Size: 1210 5685
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6895
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5506 136
Size: 107 122 71 631 605 3834 1525
Fragments arranged by size:
3834 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6890 6890
Size: 6895
BlpI GC'TnA_GC
Cuts at: 4166 4312 4166
Size: 146 6749
Bme1580I G_kGCm'C
Cuts at: 1790 2369 3234 3281 3443 3751 4956 6112 1790
Size: 579 865 47 162 308 1205 1156 2573
Fragments arranged by size:
2573 1205 1156 865 579 308 162 47
BmgBI CAC'GTC
Cuts at: 1318 2938 1318
Size: 1620 5275
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2652 3249 3446
Size: 416 53 83 399 24 1235 597 197
Cuts at: 3446 4626 5584 442
Size: 1180 958 1753
Fragments arranged by size:
1753 1235 1180 958 597 416 399 197
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6895
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2843 3035 3380 3506 3624 3828 3904
Size: 974 630 192 345 126 118 204 76
Cuts at: 3904 5555 1239
Size: 1651 2579
Fragments arranged by size:
2579 1651 974 630 345 204 192 126
118 76
Bpu10I CC'TnA_GC
Cuts at: 2420 3584 2420
Size: 1164 5731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5020 5885 6183 5020
Size: 865 298 5732
Fragments arranged by size:
5732 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3167 3182 3211 3524 3815 5558 882
Size: 617 1668 15 29 313 291 1743 2219
Fragments arranged by size:
2219 1743 1668 617 313 291 29 15
BsaAI yAC'GTr
Cuts at: 493 1204 4686 493
Size: 711 3482 2702
Fragments arranged by size:
3482 2702 711
BsaBI GATnn'nnATC
Cuts at: 6889 6889
Size: 6895
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3039 4702 5084 5719
Size: 53 83 186 936 1506 1663 382 635
Cuts at: 5719 275
Size: 1451
Fragments arranged by size:
1663 1506 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2475 3366 5328 5726 6000 6147 6558 2475
Size: 891 1962 398 274 147 411 2812
Fragments arranged by size:
2812 1962 891 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2140 2170 2984 3014 3440
Size: 30 165 30 737 30 814 30 426
Cuts at: 3440 3470 3800 3830 1178
Size: 30 330 30 4243
Fragments arranged by size:
4243 814 737 426 330 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2133 2414 2417 2840 2843 2864 2940 3461
Size: 947 281 3 423 3 21 76 521
Cuts at: 3461 3790 3904 3907 3983 1186
Size: 329 114 3 76 4098
Fragments arranged by size:
4098 947 521 423 329 281 114 76
76 21 3 3 3
BseYI C'CCAG_C
Cuts at: 2270 2380 2857 2884 3042 3430 3836 4061 6098
Size: 110 477 27 158 388 406 225 2037
Cuts at: 6098 6594 2270
Size: 496 2571
Fragments arranged by size:
2571 2037 496 477 406 388 225 158
110 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3185 3698 1163
Size: 573 1449 513 4360
Fragments arranged by size:
4360 1449 573 513
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2132 3898 4345 5106 6134 1540
Size: 275 186 131 1766 447 761 1028 2301
Fragments arranged by size:
2301 1766 1028 761 447 275 186 131
BsiHKAI G_wGCw'C
Cuts at: 3408 4956 5041 6112 3408
Size: 1548 85 1071 4191
Fragments arranged by size:
4191 1548 1071 85
BsiWI C'GTAC_G
Cuts at: 3511 3511
Size: 6895
BsmI GAATG_Cn'
Cuts at: 4406 4499 4406
Size: 93 6802
BsmBI CGTCTCn'nnnn_
Cuts at: 3030 3030
Size: 6895
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2369 2858 3234 3281 3408 3443 3702
Size: 399 579 489 376 47 127 35 259
Cuts at: 3702 3725 3751 4061 4214 4956 5041 6112 1391
Size: 23 26 310 153 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 579 489 399 376 310
259 153 127 85 47 35 26 23
BspEI T'CCGG_A
Cuts at: 6558 6558
Size: 6895
BspHI T'CATG_A
Cuts at: 3876 4092 4784 6514 3876
Size: 216 692 1730 4257
Fragments arranged by size:
4257 1730 692 216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2889 3157 4250 844
Size: 2045 268 1093 3489
Fragments arranged by size:
3489 2045 1093 268
BsrBI CCG'CTC
Cuts at: 1394 1823 2134 2626 3912 4782 1394
Size: 429 311 492 1286 870 3507
Fragments arranged by size:
3507 1286 870 492 429 311
BsrDI GCAATG_nn'
Cuts at: 65 1248 5390 5564 65
Size: 1183 4142 174 1396
Fragments arranged by size:
4142 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2127 2519 5539 5726 1700
Size: 110 317 392 3020 187 2869
Fragments arranged by size:
3020 2869 392 317 187 110
BsrGI T'GTAC_A
Cuts at: 96 2970 96
Size: 2874 4021
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6895
BssSI C'ACGA_G
Cuts at: 4955 5967 4955
Size: 1012 5883
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6895
BstBI TT'CG_AA
Cuts at: 5699 5699
Size: 6895
BstEII G'GTnAC_C
Cuts at: 1184 3616 1184
Size: 2432 4463
BstYI r'GATC_y
Cuts at: 1972 2628 4225 4592 4977 4994 6446 6532 6544
Size: 656 1597 367 385 17 1452 86 12
Cuts at: 6544 6890 1972
Size: 346 1977
Fragments arranged by size:
1977 1597 1452 656 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2310 2597 2310
Size: 287 6608
BtgI C'CryG_G
Cuts at: 513 1065 2944 3472 3525 3534 3963 513
Size: 552 1879 528 53 9 429 3445
Fragments arranged by size:
3445 1879 552 528 429 53 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2361 487
Size: 683 56 1135 5021
Fragments arranged by size:
5021 1135 683 56
BtsI GCAGTG_nn'
Cuts at: 2029 2703 2900 3213 3942 4411 5205 5225 2029
Size: 674 197 313 729 469 794 20 3699
Fragments arranged by size:
3699 794 729 674 469 313 197 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6895
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6860
DraI TTT'AAA
Cuts at: 4199 4199
Size: 6895
DraIII CAC_nnn'GTG
Cuts at: 3755 3755
Size: 6895
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5902 809
Size: 507 4586 1802
Fragments arranged by size:
4586 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2011 2097 2129
Size: 54 1425 50 275 18 181 86 32
Cuts at: 2129 3084 3544 3895 4342 5231 6860 8
Size: 955 460 351 447 889 1629 43
Fragments arranged by size:
1629 1425 955 889 460 447 351 275
181 86 54 50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 2129 3895 4342 1537
Size: 275 317 1766 447 4090
Fragments arranged by size:
4090 1766 447 317 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2214 2403 3707 4825 1353
Size: 861 189 1304 1118 3423
Fragments arranged by size:
3423 1304 1118 861 189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5482 5856 6002 387
Size: 1064 138 3893 374 146 1280
Fragments arranged by size:
3893 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3406 3406
Size: 6895
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2213 2630 2843 3035 3380 3506
Size: 393 464 117 417 213 192 345 126
Cuts at: 3506 3624 3828 3904 4958 5555 6342 6610 1239
Size: 118 204 76 1054 597 787 268 1524
Fragments arranged by size:
1524 1054 787 597 464 417 393 345
268 213 204 192 126 118 117 76
EcoNI CCTnn'n_nnAGG
Cuts at: 2476 2492 2825 2476
Size: 16 333 6546
Fragments arranged by size:
6546 333 16
EcoO109I rG'GnC_Cy
Cuts at: 2234 2350 2469 3277 2234
Size: 116 119 808 5852
Fragments arranged by size:
5852 808 119 116
EcoRI G'AATT_C
Cuts at: 3284 4204 4582 3284
Size: 920 378 5597
Fragments arranged by size:
5597 920 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2890 3646 2890
Size: 756 6139
FspI TGC'GCA
Cuts at: 5401 5401
Size: 6895
HaeII r_GCGC'y
Cuts at: 1678 6042 1678
Size: 4364 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2698 2730 3179 3211 3749
Size: 32 135 32 1126 32 449 32 538
Cuts at: 3749 3781 3800 3812 3832 3844 4087 4119 5536
Size: 32 19 12 20 12 243 32 1417
Cuts at: 5536 5568 5610 5642 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1417 1126 538 449 243 135 42
32 32 32 32 32 32 32 32
20 19 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2773 4239 4485 669
Size: 988 281 835 1466 246 3079
Fragments arranged by size:
3079 1466 988 835 281 246
HindIII A'AGCT_T
Cuts at: 748 2213 2651 748
Size: 1465 438 4992
Fragments arranged by size:
4992 1465 438
HpaI GTT'AAC
Cuts at: 4485 4485
Size: 6895
KpnI G_GTAC'C
Cuts at: 4220 4220
Size: 6895
MfeI C'AATT_G
Cuts at: 4494 4494
Size: 6895
MluI A'CGCG_T
Cuts at: 6771 6771
Size: 6895
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2154 3321 6009 6193 1305
Size: 849 1167 2688 184 2007
Fragments arranged by size:
2688 2007 1167 849 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 3086 10
Size: 54 1425 343 267 987 3819
Fragments arranged by size:
3819 1425 987 343 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3161 4853 5212 5371 518
Size: 648 1995 1692 359 159 2042
Fragments arranged by size:
2042 1995 1692 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3588 4076 4311 4988 6136 6381 1606
Size: 1982 488 235 677 1148 245 2120
Fragments arranged by size:
2120 1982 1148 677 488 245 235
NcoI C'CATG_G
Cuts at: 513 1065 3525 3534 3963 513
Size: 552 2460 9 429 3445
Fragments arranged by size:
3445 2460 552 429 9
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6895
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6895
NotI GC'GGCC_GC
Cuts at: 4342 4342
Size: 6895
NspI r_CATG'y
Cuts at: 1132 1229 3152 4253 5798 1132
Size: 97 1923 1101 1545 2229
Fragments arranged by size:
2229 1923 1545 1101 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6895
PciI A'CATG_T
Cuts at: 5794 5794
Size: 6895
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2330 3526 3791 1108
Size: 1222 1196 265 4212
Fragments arranged by size:
4212 1222 1196 265
PfoI T'CCnGG_A
Cuts at: 2982 3384 3483 2982
Size: 402 99 6394
Fragments arranged by size:
6394 402 99
PmeI GTTT'AAAC
Cuts at: 4199 4199
Size: 6895
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4964 4996 6477 6509 4964
Size: 32 1481 32 5350
Fragments arranged by size:
5350 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2234 2350 2234
Size: 116 6779
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6895
PsiI TTA'TAA
Cuts at: 4465 4465
Size: 6895
PspOMI G'GGCC_C
Cuts at: 3277 3439 3277
Size: 162 6733
PspXI vC'TCGA_Gb
Cuts at: 2447 2447
Size: 6895
PstI C_TGCA'G
Cuts at: 830 2907 3949 4247 830
Size: 2077 1042 298 3478
Fragments arranged by size:
3478 2077 1042 298
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6895
PvuII CAG'CTG
Cuts at: 4076 4076
Size: 6895
SacI G_AGCT'C
Cuts at: 3408 3408
Size: 6895
SalI G'TCGA_C
Cuts at: 1936 4237 1936
Size: 2301 4594
SanDI GG'GwC_CC
Cuts at: 2234 2234
Size: 6895
SapI GCTCTTCn'nnn_
Cuts at: 2214 3707 2214
Size: 1493 5402
SbfI CC_TGCA'GG
Cuts at: 4247 4247
Size: 6895
ScaI AGT'ACT
Cuts at: 5143 6855 5143
Size: 1712 5183
SfcI C'TryA_G
Cuts at: 826 1045 2903 3945 4033 4243 5378 6059 6250
Size: 219 1858 1042 88 210 1135 681 191
Cuts at: 6250 826
Size: 1471
Fragments arranged by size:
1858 1471 1135 1042 681 219 210 191
88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6895
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6895
SmaI CCC'GGG
Cuts at: 1872 4222 1872
Size: 2350 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2447 4999 5900 6162 820
Size: 197 926 504 2552 901 262 1553
Fragments arranged by size:
2552 1553 926 901 504 262 197
SnaBI TAC'GTA
Cuts at: 493 4686 493
Size: 4193 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6895
SphI G_CATG'C
Cuts at: 1132 4253 1132
Size: 3121 3774
SspI AAT'ATT
Cuts at: 5 52 4819 5
Size: 47 4767 2081
Fragments arranged by size:
4767 2081 47
StuI AGG'CCT
Cuts at: 1517 2735 2931 1517
Size: 1218 196 5481
Fragments arranged by size:
5481 1218 196
StyI C'CwwG_G
Cuts at: 513 1065 2634 2730 3525 3534 3963 4334 513
Size: 552 1569 96 795 9 429 371 3074
Fragments arranged by size:
3074 1569 795 552 429 371 96 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3790 4922 4939 5092 5277 5722 325
Size: 1255 2210 1132 17 153 185 445 1498
Fragments arranged by size:
2210 1498 1255 1132 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2970 5141 6853
Size: 274 80 33 51 1443 993 2171 1712
Cuts at: 6853 96
Size: 138
Fragments arranged by size:
2171 1712 1443 993 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2008 2040 2140 2172 3226 3258 6855 6887 2008
Size: 32 100 32 1054 32 3597 32 2016
Fragments arranged by size:
3597 2016 1054 100 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6895
XbaI T'CTAG_A
Cuts at: 4231 4231
Size: 6895
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2818 3433 3532 4178 2818
Size: 615 99 646 5535
Fragments arranged by size:
5535 646 615 99
XhoI C'TCGA_G
Cuts at: 1943 2447 1943
Size: 504 6391
XmaI C'CCGG_G
Cuts at: 1870 4220 1870
Size: 2350 4545
XmnI GAAnn'nnTTC
Cuts at: 3872 5024 3872
Size: 1152 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4703 276
Size: 53 83 186 936 3169 2468
Fragments arranged by size:
3169 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII
SacII SexAI SfiI SfoI SrfI SwaI