Restriction map for
pFN21AE4195
(Circular) (Six-base) MAPSORT of: pFN21AE4195.seq Check: 8958 from: 1 to: 6817
pFN21AE4195 6817 bp
With 182 enzymes: SgfI *
September 21, 2016 11:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4627 278
Size: 53 83 186 936 3091 2468
Fragments arranged by size:
3091 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2159 4160 1937
Size: 222 2001 4594
Fragments arranged by size:
4594 2001 222
Acc65I G'GTAC_C
Cuts at: 4138 4138
Size: 6817
AclI AA'CG_TT
Cuts at: 1563 4944 5317 1563
Size: 3381 373 3063
Fragments arranged by size:
3381 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3027 3051 3156 3513 4019 4047 4880 6264
Size: 1395 24 105 357 506 28 833 1384
Cuts at: 6264 6532 1632
Size: 268 1917
Fragments arranged by size:
1917 1395 1384 833 506 357 268 105
28 24
AflII C'TTAA_G
Cuts at: 820 1017 2062 820
Size: 197 1045 5575
Fragments arranged by size:
5575 1045 197
AflIII A'CryG_T
Cuts at: 1203 4001 4003 5716 6693 1203
Size: 2798 2 1713 977 1327
Fragments arranged by size:
2798 1713 1327 977 2
AgeI A'CCGG_T
Cuts at: 5648 5648
Size: 6817
AhdI GACnn_n'nnGTC
Cuts at: 5546 5546
Size: 6817
AleI CACnn'nnGTG
Cuts at: 1166 2493 1166
Size: 1327 5490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6817
AlwNI CAG_nnn'CTG
Cuts at: 2177 3005 4560 6132 2177
Size: 828 1555 1572 2862
Fragments arranged by size:
2862 1572 1555 828
ApaLI G'TGCA_C
Cuts at: 3718 4874 6030 3718
Size: 1156 1156 4505
Fragments arranged by size:
4505 1156 1156
ApoI r'AATT_y
Cuts at: 1493 2037 2662 2677 2932 2941 3118 3538 3663
Size: 544 625 15 255 9 177 420 125
Cuts at: 3663 4126 4352 4504 6613 1493
Size: 463 226 152 2109 1697
Fragments arranged by size:
2109 1697 625 544 463 420 255 226
177 152 125 15 9
AseI AT'TA_AT
Cuts at: 160 1032 5371 160
Size: 872 4339 1606
Fragments arranged by size:
4339 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6817
AvaI C'yCGr_G
Cuts at: 1870 1943 4142 1870
Size: 73 2199 4545
Fragments arranged by size:
4545 2199 73
BamHI G'GATC_C
Cuts at: 4147 4514 4147
Size: 367 6450
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4138 5593 618
Size: 325 217 627 2351 1455 1842
Fragments arranged by size:
2351 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3883 4136 1391
Size: 2492 253 4072
Fragments arranged by size:
4072 2492 253
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4089 928
Size: 971 2190 3656
Fragments arranged by size:
3656 2190 971
BbvCI CC'TCA_GC
Cuts at: 2308 2308
Size: 6817
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2830 2864 5008 5042 1903
Size: 34 893 34 2144 34 3678
Fragments arranged by size:
3678 2144 893 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3231 4709 5919 3231
Size: 1478 1210 4129
Fragments arranged by size:
4129 1478 1210
BclI T'GATC_A
Cuts at: 1548 3507 1548
Size: 1959 4858
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5428 136
Size: 107 122 71 631 605 3756 1525
Fragments arranged by size:
3756 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2381 2897 3263 4051 6812 2381
Size: 516 366 788 2761 2386
Fragments arranged by size:
2761 2386 788 516 366
BlpI GC'TnA_GC
Cuts at: 4234 4234
Size: 6817
Bme1580I G_kGCm'C
Cuts at: 1790 3722 4878 6034 1790
Size: 1932 1156 1156 2573
Fragments arranged by size:
2573 1932 1156 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6817
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4548 5506 442
Size: 416 53 83 399 24 3131 958 1753
Fragments arranged by size:
3131 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6817
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3869 5477 1239
Size: 2630 1608 2579
Fragments arranged by size:
2630 2579 1608
Bpu10I CC'TnA_GC
Cuts at: 2308 2308
Size: 6817
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2104 2221 3390 4942 5807 6105 2104
Size: 117 1169 1552 865 298 2816
Fragments arranged by size:
2816 1552 1169 865 298 117
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3929 4083 5480 882
Size: 617 2430 154 1397 2219
Fragments arranged by size:
2430 2219 1397 617 154
BsaAI yAC'GTr
Cuts at: 493 1204 4004 4608 493
Size: 711 2800 604 2702
Fragments arranged by size:
2800 2702 711 604
BsaBI GATnn'nnATC
Cuts at: 2112 2386 6811 2112
Size: 274 4425 2118
Fragments arranged by size:
4425 2118 274
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4624 5006 5641 275
Size: 53 83 186 936 3091 382 635 1451
Fragments arranged by size:
3091 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3456 5250 5648 5922 6069 6480 3456
Size: 1794 398 274 147 411 3793
Fragments arranged by size:
3793 1794 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2353 2383 1178
Size: 30 165 30 950 30 5612
Fragments arranged by size:
5612 950 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2446 2857 1186
Size: 1260 411 5146
Fragments arranged by size:
5146 1260 411
BseYI C'CCAG_C
Cuts at: 2312 3472 6020 6516 2312
Size: 1160 2548 496 2613
Fragments arranged by size:
2613 2548 1160 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6244
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4267 5028 6056 1540
Size: 275 186 2266 761 1028 2301
Fragments arranged by size:
2301 2266 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2233 2582 3722 3883 4878 4963 6034 2233
Size: 349 1140 161 995 85 1071 3016
Fragments arranged by size:
3016 1140 1071 995 349 161 85
BsmI GAATG_Cn'
Cuts at: 4328 4421 4328
Size: 93 6724
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 2582 3722 3883 4136 4878 4963
Size: 399 443 349 1140 161 253 742 85
Cuts at: 4963 6034 1391
Size: 1071 2174
Fragments arranged by size:
2174 1140 1071 742 443 399 349 253
161 85
BspEI T'CCGG_A
Cuts at: 6480 6480
Size: 6817
BspHI T'CATG_A
Cuts at: 2263 3504 3865 4706 6436 2263
Size: 1241 361 841 1730 2644
Fragments arranged by size:
2644 1730 1241 841 361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4172 844
Size: 3328 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4704 1394
Size: 429 2881 3507
Fragments arranged by size:
3507 2881 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5312 5486 65
Size: 1183 4064 174 1396
Fragments arranged by size:
4064 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5461 5648 1700
Size: 110 3651 187 2869
Fragments arranged by size:
3651 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2291 96
Size: 2195 4622
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6817
BssSI C'ACGA_G
Cuts at: 4877 5889 4877
Size: 1012 5805
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6817
BstBI TT'CG_AA
Cuts at: 5621 5621
Size: 6817
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6817
BstYI r'GATC_y
Cuts at: 1972 2381 2897 3263 3586 4051 4147 4514 4899
Size: 409 516 366 323 465 96 367 385
Cuts at: 4899 4916 6368 6454 6466 6812 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 516 465 409 385 367 366
346 323 96 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3155 513
Size: 552 939 1151 4175
Fragments arranged by size:
4175 1151 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6078
Fragments arranged by size:
6078 683 56
BtsI GCAGTG_nn'
Cuts at: 3376 4333 5127 5147 3376
Size: 957 794 20 5046
Fragments arranged by size:
5046 957 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6817
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3276 3311 529
Size: 35 2712 35 4035
Fragments arranged by size:
4035 2712 35 35
DraI TTT'AAA
Cuts at: 2103 4121 2103
Size: 2018 4799
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5824 809
Size: 507 4508 1802
Fragments arranged by size:
4508 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4264 5153 6782
Size: 54 1425 50 275 18 2434 889 1629
Cuts at: 6782 8
Size: 43
Fragments arranged by size:
2434 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4264 1537
Size: 275 2452 4090
Fragments arranged by size:
4090 2452 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2134 3214 3274 4038 4747 1353
Size: 781 1080 60 764 709 3423
Fragments arranged by size:
3423 1080 781 764 709 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2848 5404 5778 5924 387
Size: 1064 138 1259 2556 374 146 1280
Fragments arranged by size:
2556 1280 1259 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3881 3881
Size: 6817
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3027 3051 3156 3513 3869 4019 4047
Size: 393 1395 24 105 357 356 150 28
Cuts at: 4047 4880 5477 6264 6532 1239
Size: 833 597 787 268 1524
Fragments arranged by size:
1524 1395 833 787 597 393 357 356
268 150 105 28 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3626 3626
Size: 6817
EcoRI G'AATT_C
Cuts at: 2932 3538 4126 4504 2932
Size: 606 588 378 5245
Fragments arranged by size:
5245 606 588 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2145 3594 2145
Size: 1449 5368
FspI TGC'GCA
Cuts at: 5323 5323
Size: 6817
HaeII r_GCGC'y
Cuts at: 1678 5964 1678
Size: 4286 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2353 2385 3242 3274 5458
Size: 32 135 32 781 32 857 32 2184
Cuts at: 5458 5490 5532 5564 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2184 857 781 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4161 4407 669
Size: 988 281 2223 246 3079
Fragments arranged by size:
3079 2223 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3033 3961 748
Size: 2285 928 3604
Fragments arranged by size:
3604 2285 928
HpaI GTT'AAC
Cuts at: 4407 4407
Size: 6817
KpnI G_GTAC'C
Cuts at: 4142 4142
Size: 6817
MfeI C'AATT_G
Cuts at: 2615 4416 2615
Size: 1801 5016
MluI A'CGCG_T
Cuts at: 6693 6693
Size: 6817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2305 3515 3816 5931 6115 1305
Size: 1000 1210 301 2115 184 2007
Fragments arranged by size:
2115 2007 1210 1000 301 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4995
Fragments arranged by size:
4995 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2493 3715 3888 4775 5134 5293 518
Size: 648 1327 1222 173 887 359 159 2042
Fragments arranged by size:
2042 1327 1222 887 648 359 173 159
MspA1I CmG'CkG
Cuts at: 1606 2312 3545 3680 4233 4910 6058 6303 1606
Size: 706 1233 135 553 677 1148 245 2120
Fragments arranged by size:
2120 1233 1148 706 677 553 245 135
NcoI C'CATG_G
Cuts at: 513 1065 2004 3155 513
Size: 552 939 1151 4175
Fragments arranged by size:
4175 1151 939 552
NdeI CA'TA_TG
Cuts at: 387 2518 4044 387
Size: 2131 1526 3160
Fragments arranged by size:
3160 2131 1526
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6817
NotI GC'GGCC_GC
Cuts at: 4264 4264
Size: 6817
NspI r_CATG'y
Cuts at: 1132 1229 2875 4175 5720 1132
Size: 97 1646 1300 1545 2229
Fragments arranged by size:
2229 1646 1545 1300 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6817
PciI A'CATG_T
Cuts at: 5716 5716
Size: 6817
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3479 1108
Size: 2371 4446
PmeI GTTT'AAAC
Cuts at: 4121 4121
Size: 6817
PmlI CAC'GTG
Cuts at: 4004 4004
Size: 6817
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4886 4918 6399 6431 4886
Size: 32 1481 32 5272
Fragments arranged by size:
5272 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6817
PsiI TTA'TAA
Cuts at: 4387 4387
Size: 6817
PstI C_TGCA'G
Cuts at: 830 3361 4169 830
Size: 2531 808 3478
Fragments arranged by size:
3478 2531 808
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6817
PvuII CAG'CTG
Cuts at: 2312 3545 3680 2312
Size: 1233 135 5449
Fragments arranged by size:
5449 1233 135
SacI G_AGCT'C
Cuts at: 3883 3883
Size: 6817
SalI G'TCGA_C
Cuts at: 1936 4159 1936
Size: 2223 4594
SbfI CC_TGCA'GG
Cuts at: 4169 4169
Size: 6817
ScaI AGT'ACT
Cuts at: 5065 6777 5065
Size: 1712 5105
SfcI C'TryA_G
Cuts at: 826 1045 3357 4165 5300 5981 6172 826
Size: 219 2312 808 1135 681 191 1471
Fragments arranged by size:
2312 1471 1135 808 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6817
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6817
SmaI CCC'GGG
Cuts at: 1872 4144 1872
Size: 2272 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2062 2119 2236 3405 4921 5822
Size: 197 926 119 57 117 1169 1516 901
Cuts at: 5822 6084 820
Size: 262 1553
Fragments arranged by size:
1553 1516 1169 926 901 262 197 119
117 57
SnaBI TAC'GTA
Cuts at: 493 4608 493
Size: 4115 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6817
SphI G_CATG'C
Cuts at: 1132 4175 1132
Size: 3043 3774
SspI AAT'ATT
Cuts at: 5 52 4741 5
Size: 47 4689 2081
Fragments arranged by size:
4689 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6817
StyI C'CwwG_G
Cuts at: 513 1065 2004 3155 4256 513
Size: 552 939 1151 1101 3074
Fragments arranged by size:
3074 1151 1101 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3509 4844 4861 5014 5199 5644 325
Size: 1255 1929 1335 17 153 185 445 1498
Fragments arranged by size:
1929 1498 1335 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2291 2300 5063
Size: 274 80 33 51 1443 314 9 2763
Cuts at: 5063 6775 96
Size: 1712 138
Fragments arranged by size:
2763 1712 1443 314 274 138 80 51
33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6777 6809 6777
Size: 32 6785
Tth111I GACn'n_nGTC
Cuts at: 1316 3500 1316
Size: 2184 4633
XbaI T'CTAG_A
Cuts at: 3200 3260 4153 3200
Size: 60 893 5864
Fragments arranged by size:
5864 893 60
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6817
XmaI C'CCGG_G
Cuts at: 1870 4142 1870
Size: 2272 4545
XmnI GAAnn'nnTTC
Cuts at: 2038 2455 3297 4946 2038
Size: 417 842 1649 3909
Fragments arranged by size:
3909 1649 842 417
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4625 276
Size: 53 83 186 936 3091 2468
Fragments arranged by size:
3091 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI