Restriction map for
pFN21AE4194
(Circular) (Six-base) MAPSORT of: pFN21AE4194.seq Check: 6718 from: 1 to: 6814
pFN21AE4194 6814 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2228 2228
Size: 6814
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4624 278
Size: 53 83 186 936 3088 2468
Fragments arranged by size:
3088 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3660 4157 1937
Size: 1723 497 4594
Fragments arranged by size:
4594 1723 497
Acc65I G'GTAC_C
Cuts at: 4135 4135
Size: 6814
AclI AA'CG_TT
Cuts at: 1563 4941 5314 1563
Size: 3378 373 3063
Fragments arranged by size:
3378 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2475 2615 2771 3338 3722 4877 6261 6529
Size: 843 140 156 567 384 1155 1384 268
Cuts at: 6529 1632
Size: 1917
Fragments arranged by size:
1917 1384 1155 843 567 384 268 156
140
AflII C'TTAA_G
Cuts at: 820 1017 2408 820
Size: 197 1391 5226
Fragments arranged by size:
5226 1391 197
AflIII A'CryG_T
Cuts at: 1203 5713 6690 1203
Size: 4510 977 1327
Fragments arranged by size:
4510 1327 977
AgeI A'CCGG_T
Cuts at: 5645 5645
Size: 6814
AhdI GACnn_n'nnGTC
Cuts at: 5543 5543
Size: 6814
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6814
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6814
AlwNI CAG_nnn'CTG
Cuts at: 2015 2923 4557 6129 2015
Size: 908 1634 1572 2700
Fragments arranged by size:
2700 1634 1572 908
ApaI G_GGCC'C
Cuts at: 4069 4069
Size: 6814
ApaLI G'TGCA_C
Cuts at: 4871 6027 4871
Size: 1156 5658
ApoI r'AATT_y
Cuts at: 1493 2677 4058 4123 4349 4501 6610 1493
Size: 1184 1381 65 226 152 2109 1697
Fragments arranged by size:
2109 1697 1381 1184 226 152 65
AseI AT'TA_AT
Cuts at: 160 1032 5368 160
Size: 872 4336 1606
Fragments arranged by size:
4336 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6814
AvaI C'yCGr_G
Cuts at: 1870 1943 4139 1870
Size: 73 2196 4545
Fragments arranged by size:
4545 2196 73
BamHI G'GATC_C
Cuts at: 4144 4511 4144
Size: 367 6447
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2418 4135 5590 618
Size: 325 217 627 631 1717 1455 1842
Fragments arranged by size:
1842 1717 1455 631 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4069 4133 1391
Size: 2678 64 4072
Fragments arranged by size:
4072 2678 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3939 928
Size: 971 2040 3803
Fragments arranged by size:
3803 2040 971
BbvCI CC'TCA_GC
Cuts at: 2248 2248
Size: 6814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5005 5039 1903
Size: 34 3068 34 3678
Fragments arranged by size:
3678 3068 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4706 5916 4706
Size: 1210 5604
BclI T'GATC_A
Cuts at: 1548 2575 1548
Size: 1027 5787
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3053 5425 136
Size: 107 122 71 631 605 1381 2372 1525
Fragments arranged by size:
2372 1525 1381 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3060 6809 3060
Size: 3749 3065
BlpI GC'TnA_GC
Cuts at: 3287 4231 3287
Size: 944 5870
Bme1580I G_kGCm'C
Cuts at: 1790 4069 4875 6031 1790
Size: 2279 806 1156 2573
Fragments arranged by size:
2573 2279 1156 806
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6814
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2040 3363 3429
Size: 416 53 83 399 24 623 1323 66
Cuts at: 3429 3999 4545 5503 442
Size: 570 546 958 1753
Fragments arranged by size:
1753 1323 958 623 570 546 416 399
83 66 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2425 2517 3188 3689 3701 3942 5474 1239
Size: 1186 92 671 501 12 241 1532 2579
Fragments arranged by size:
2579 1532 1186 671 501 241 92 12
Bpu10I CC'TnA_GC
Cuts at: 2248 2248
Size: 6814
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2978 3131 4939 5804 6102 2978
Size: 153 1808 865 298 3690
Fragments arranged by size:
3690 1808 865 298 153
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2099 2322 2409 5477 882
Size: 617 600 223 87 3068 2219
Fragments arranged by size:
3068 2219 617 600 223 87
BsaAI yAC'GTr
Cuts at: 493 1204 3758 4605 493
Size: 711 2554 847 2702
Fragments arranged by size:
2702 2554 847 711
BsaBI GATnn'nnATC
Cuts at: 2574 6808 2574
Size: 4234 2580
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4621 5003 5638 275
Size: 53 83 186 936 3088 382 635 1451
Fragments arranged by size:
3088 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2053 5247 5645 5919 6066 6477 2053
Size: 3194 398 274 147 411 2390
Fragments arranged by size:
3194 2390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2544 2574 3307 3337 1178
Size: 30 165 30 1141 30 733 30 4655
Fragments arranged by size:
4655 1141 733 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2372 3375 1186
Size: 1186 1003 4625
Fragments arranged by size:
4625 1186 1003
BseYI C'CCAG_C
Cuts at: 3486 3491 6017 6513 3486
Size: 5 2526 496 3787
Fragments arranged by size:
3787 2526 496 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2109 3459 3792 1163
Size: 573 373 1350 333 4185
Fragments arranged by size:
4185 1350 573 373 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4264 5025 6053 1540
Size: 275 186 2263 761 1028 2301
Fragments arranged by size:
2301 2263 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 4875 4960 6031 2211
Size: 2664 85 1071 2994
Fragments arranged by size:
2994 2664 1071 85
BsmI GAATG_Cn'
Cuts at: 4325 4418 4325
Size: 93 6721
BsmBI CGTCTCn'nnnn_
Cuts at: 3191 3191
Size: 6814
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 4069 4133 4875 4960 6031 1391
Size: 399 421 1858 64 742 85 1071 2174
Fragments arranged by size:
2174 1858 1071 742 421 399 85 64
BspEI T'CCGG_A
Cuts at: 2053 6477 2053
Size: 4424 2390
BspHI T'CATG_A
Cuts at: 4703 6433 4703
Size: 1730 5084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2228 3400 4169 844
Size: 1384 1172 769 3489
Fragments arranged by size:
3489 1384 1172 769
BsrBI CCG'CTC
Cuts at: 1394 1823 3450 3829 4701 1394
Size: 429 1627 379 872 3507
Fragments arranged by size:
3507 1627 872 429 379
BsrDI GCAATG_nn'
Cuts at: 65 1248 2610 5309 5483 65
Size: 1183 1362 2699 174 1396
Fragments arranged by size:
2699 1396 1362 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5458 5645 1700
Size: 110 3648 187 2869
Fragments arranged by size:
3648 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2617 96
Size: 2521 4293
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6814
BssSI C'ACGA_G
Cuts at: 4874 5886 4874
Size: 1012 5802
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6814
BstBI TT'CG_AA
Cuts at: 5618 5618
Size: 6814
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6814
BstYI r'GATC_y
Cuts at: 1972 2920 3060 3442 3675 4144 4511 4896 4913
Size: 948 140 382 233 469 367 385 17
Cuts at: 4913 6365 6451 6463 6809 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 948 469 385 382 367 346
233 140 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3423 3523 3565 3765 3780 513
Size: 552 939 1419 100 42 200 15 3547
Fragments arranged by size:
3547 1419 939 552 200 100 42 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6075
Fragments arranged by size:
6075 683 56
BtsI GCAGTG_nn'
Cuts at: 2317 4330 5124 5144 2317
Size: 2013 794 20 3987
Fragments arranged by size:
3987 2013 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6814
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6779
DraI TTT'AAA
Cuts at: 4118 4118
Size: 6814
DraIII CAC_nnn'GTG
Cuts at: 2263 2263
Size: 6814
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5821 809
Size: 507 4505 1802
Fragments arranged by size:
4505 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2340 4261
Size: 54 1425 50 275 18 204 306 1921
Cuts at: 4261 5150 6779 8
Size: 889 1629 43
Fragments arranged by size:
1921 1629 1425 889 306 275 204 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4261 1537
Size: 275 2449 4090
Fragments arranged by size:
4090 2449 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2451 2593 2796 2956 3027 4744 1353
Size: 1098 142 203 160 71 1717 3423
Fragments arranged by size:
3423 1717 1098 203 160 142 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5401 5775 5921 387
Size: 1064 138 3812 374 146 1280
Fragments arranged by size:
3812 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2425 2475 2517 2615 2771 3188 3338
Size: 393 793 50 42 98 156 417 150
Cuts at: 3338 3689 3701 3722 3942 4877 5474 6261 6529
Size: 351 12 21 220 935 597 787 268
Cuts at: 6529 1239
Size: 1524
Fragments arranged by size:
1524 935 793 787 597 417 393 351
268 220 156 150 98 50 42 21
12
EcoNI CCTnn'n_nnAGG
Cuts at: 3606 3606
Size: 6814
EcoO109I rG'GnC_Cy
Cuts at: 2731 2731
Size: 6814
EcoRI G'AATT_C
Cuts at: 4123 4501 4123
Size: 378 6436
FspI TGC'GCA
Cuts at: 5320 5320
Size: 6814
HaeII r_GCGC'y
Cuts at: 1678 5961 1678
Size: 4283 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2374 2406 3050 3082 3434
Size: 32 135 32 802 32 644 32 352
Cuts at: 3434 3466 5455 5487 5529 5561 1373
Size: 32 1989 32 42 32 2626
Fragments arranged by size:
2626 1989 802 644 352 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3153 4158 4404 669
Size: 988 281 1215 1005 246 3079
Fragments arranged by size:
3079 1215 1005 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2506 2597 2942 748
Size: 1758 91 345 4620
Fragments arranged by size:
4620 1758 345 91
HpaI GTT'AAC
Cuts at: 4404 4404
Size: 6814
KpnI G_GTAC'C
Cuts at: 4139 4139
Size: 6814
MfeI C'AATT_G
Cuts at: 4413 4413
Size: 6814
MluI A'CGCG_T
Cuts at: 6690 6690
Size: 6814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2542 4127 5928 6112 1305
Size: 1237 1585 1801 184 2007
Fragments arranged by size:
2007 1801 1585 1237 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2036 2342 10
Size: 54 1425 343 204 306 4482
Fragments arranged by size:
4482 1425 343 306 204 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3428 4772 5131 5290 518
Size: 648 2262 1344 359 159 2042
Fragments arranged by size:
2262 2042 1344 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2639 4230 4907 6055 6300 1606
Size: 1033 1591 677 1148 245 2120
Fragments arranged by size:
2120 1591 1148 1033 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3423 3523 3765 3780 513
Size: 552 939 1419 100 242 15 3547
Fragments arranged by size:
3547 1419 939 552 242 100 15
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6814
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6814
NotI GC'GGCC_GC
Cuts at: 4261 4261
Size: 6814
NspI r_CATG'y
Cuts at: 1132 1229 3580 3956 4172 5717 1132
Size: 97 2351 376 216 1545 2229
Fragments arranged by size:
2351 2229 1545 376 216 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6814
PciI A'CATG_T
Cuts at: 5713 5713
Size: 6814
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2540 1108
Size: 1432 5382
PfoI T'CCnGG_A
Cuts at: 2441 2733 3678 3937 2441
Size: 292 945 259 5318
Fragments arranged by size:
5318 945 292 259
PmeI GTTT'AAAC
Cuts at: 4118 4118
Size: 6814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4883 4915 6396 6428 4883
Size: 32 1481 32 5269
Fragments arranged by size:
5269 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2731 2731
Size: 6814
PshAI GACnn'nnGTC
Cuts at: 1613 2091 4089 1613
Size: 478 1998 4338
Fragments arranged by size:
4338 1998 478
PsiI TTA'TAA
Cuts at: 4384 4384
Size: 6814
PspOMI G'GGCC_C
Cuts at: 4065 4065
Size: 6814
PstI C_TGCA'G
Cuts at: 830 4166 830
Size: 3336 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6814
PvuII CAG'CTG
Cuts at: 2639 2639
Size: 6814
SalI G'TCGA_C
Cuts at: 1936 4156 1936
Size: 2220 4594
SapI GCTCTTCn'nnn_
Cuts at: 2593 2593
Size: 6814
SbfI CC_TGCA'GG
Cuts at: 4166 4166
Size: 6814
ScaI AGT'ACT
Cuts at: 3844 5062 6774 3844
Size: 1218 1712 3884
Fragments arranged by size:
3884 1712 1218
SexAI A'CCwGG_T
Cuts at: 2259 2259
Size: 6814
SfcI C'TryA_G
Cuts at: 826 1045 2523 4162 5297 5978 6169 826
Size: 219 1478 1639 1135 681 191 1471
Fragments arranged by size:
1639 1478 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6814
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6814
SmaI CCC'GGG
Cuts at: 1872 4141 1872
Size: 2269 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 2993 3146 4918 5819 6081
Size: 197 926 465 585 153 1772 901 262
Cuts at: 6081 820
Size: 1553
Fragments arranged by size:
1772 1553 926 901 585 465 262 197
153
SnaBI TAC'GTA
Cuts at: 493 4605 493
Size: 4112 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6814
SphI G_CATG'C
Cuts at: 1132 4172 1132
Size: 3040 3774
SspI AAT'ATT
Cuts at: 5 52 4738 5
Size: 47 4686 2081
Fragments arranged by size:
4686 2081 47
StuI AGG'CCT
Cuts at: 1517 3774 1517
Size: 2257 4557
StyI C'CwwG_G
Cuts at: 513 1065 2004 2178 3423 3523 3765 3780 4253
Size: 552 939 174 1245 100 242 15 473
Cuts at: 4253 513
Size: 3074
Fragments arranged by size:
3074 1245 939 552 473 242 174 100
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2391 4841 4858 5011 5196 5641 325
Size: 1255 811 2450 17 153 185 445 1498
Fragments arranged by size:
2450 1498 1255 811 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2617 3842 5060
Size: 274 80 33 51 1443 640 1225 1218
Cuts at: 5060 6772 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1225 1218 640 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2544 2576 3307 3339 6774 6806 2544
Size: 32 731 32 3435 32 2552
Fragments arranged by size:
3435 2552 731 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6814
XbaI T'CTAG_A
Cuts at: 4150 4150
Size: 6814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3376 3376
Size: 6814
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6814
XmaI C'CCGG_G
Cuts at: 1870 4139 1870
Size: 2269 4545
XmnI GAAnn'nnTTC
Cuts at: 3946 4943 3946
Size: 997 5817
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4622 276
Size: 53 83 186 936 3088 2468
Fragments arranged by size:
3088 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI