Restriction map for
pFN21AE4192
(Circular) (Six-base) MAPSORT of: pFN21AE4192.seq Check: 5451 from: 1 to: 6784
pFN21AE4192 6784 bp
With 182 enzymes: SgfI *
March 28, 2012 13:06 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4594 278
Size: 53 83 186 936 3058 2468
Fragments arranged by size:
3058 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2800 4127 1937
Size: 863 1327 4594
Fragments arranged by size:
4594 1327 863
Acc65I G'GTAC_C
Cuts at: 4105 4105
Size: 6784
AclI AA'CG_TT
Cuts at: 1563 4911 5284 1563
Size: 3348 373 3063
Fragments arranged by size:
3348 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2220 4847 6231 6499 1632
Size: 588 2627 1384 268 1917
Fragments arranged by size:
2627 1917 1384 588 268
AfeI AGC'GCT
Cuts at: 2619 2619
Size: 6784
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6587
AflIII A'CryG_T
Cuts at: 1203 5683 6660 1203
Size: 4480 977 1327
Fragments arranged by size:
4480 1327 977
AgeI A'CCGG_T
Cuts at: 2951 5615 2951
Size: 2664 4120
AhdI GACnn_n'nnGTC
Cuts at: 5513 5513
Size: 6784
AleI CACnn'nnGTG
Cuts at: 1166 3933 1166
Size: 2767 4017
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2961 1184
Size: 1777 5007
AlwNI CAG_nnn'CTG
Cuts at: 3746 4527 6099 3746
Size: 781 1572 4431
Fragments arranged by size:
4431 1572 781
ApaLI G'TGCA_C
Cuts at: 4841 5997 4841
Size: 1156 5628
ApoI r'AATT_y
Cuts at: 1493 2276 2415 2808 3243 3403 3542 3823 4093
Size: 783 139 393 435 160 139 281 270
Cuts at: 4093 4319 4471 6580 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 783 435 393 281 270 226
160 152 139 139
AseI AT'TA_AT
Cuts at: 160 1032 3615 5338 160
Size: 872 2583 1723 1606
Fragments arranged by size:
2583 1723 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6784
AvaI C'yCGr_G
Cuts at: 1870 1943 3312 4109 1870
Size: 73 1369 797 4545
Fragments arranged by size:
4545 1369 797 73
BamHI G'GATC_C
Cuts at: 4114 4481 4114
Size: 367 6417
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4105 5560 618
Size: 325 217 627 2318 1455 1842
Fragments arranged by size:
2318 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4103 1391
Size: 2712 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2461 2919 928
Size: 971 562 458 4793
Fragments arranged by size:
4793 971 562 458
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4975 5009 1903
Size: 34 3038 34 3678
Fragments arranged by size:
3678 3038 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2036 4676 5886 2036
Size: 2640 1210 2934
Fragments arranged by size:
2934 2640 1210
BclI T'GATC_A
Cuts at: 1548 2188 1548
Size: 640 6144
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5395 136
Size: 107 122 71 631 605 3723 1525
Fragments arranged by size:
3723 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6779 6779
Size: 6784
BlpI GC'TnA_GC
Cuts at: 3267 4201 3267
Size: 934 5850
Bme1580I G_kGCm'C
Cuts at: 1790 4845 6001 1790
Size: 3055 1156 2573
Fragments arranged by size:
3055 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2753 1318
Size: 1435 5349
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4515 5473 442
Size: 416 53 83 399 24 3098 958 1753
Fragments arranged by size:
3098 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6784
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5444 1239
Size: 4205 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3795 4909 5774 6072 3795
Size: 1114 865 298 4507
Fragments arranged by size:
4507 1114 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5447 882
Size: 617 3948 2219
Fragments arranged by size:
3948 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4575 493
Size: 711 3371 2702
Fragments arranged by size:
3371 2702 711
BsaBI GATnn'nnATC
Cuts at: 6778 6778
Size: 6784
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4591 4973 5608 275
Size: 53 83 186 936 3058 382 635 1451
Fragments arranged by size:
3058 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2951 5217 5615 5889 6036 6447 2951
Size: 2266 398 274 147 411 3288
Fragments arranged by size:
3288 2266 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6559
Fragments arranged by size:
6559 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2004 1186
Size: 818 5966
BseYI C'CCAG_C
Cuts at: 5987 6483 5987
Size: 496 6288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3674 3915 3981 1163
Size: 573 1938 241 66 3966
Fragments arranged by size:
3966 1938 573 241 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4234 4995 6023 1540
Size: 275 186 2233 761 1028 2301
Fragments arranged by size:
2301 2233 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3268 4845 4930 6001 3268
Size: 1577 85 1071 4051
Fragments arranged by size:
4051 1577 1071 85
BsmI GAATG_Cn'
Cuts at: 4295 4388 4295
Size: 93 6691
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3268 4103 4845 4930 6001 1391
Size: 399 1478 835 742 85 1071 2174
Fragments arranged by size:
2174 1478 1071 835 742 399 85
BspEI T'CCGG_A
Cuts at: 6447 6447
Size: 6784
BspHI T'CATG_A
Cuts at: 4673 6403 4673
Size: 1730 5054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4139 844
Size: 3295 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4671 1394
Size: 429 2848 3507
Fragments arranged by size:
3507 2848 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2102 3882 5279 5453 65
Size: 1183 854 1780 1397 174 1396
Fragments arranged by size:
1780 1397 1396 1183 854 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2951 5428 5615 1700
Size: 110 1141 2477 187 2869
Fragments arranged by size:
2869 2477 1141 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6784
BssHII G'CGCG_C
Cuts at: 1925 3673 1925
Size: 1748 5036
BssSI C'ACGA_G
Cuts at: 4844 5856 4844
Size: 1012 5772
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2390 3434 3893 794
Size: 1596 1044 459 3685
Fragments arranged by size:
3685 1596 1044 459
BstBI TT'CG_AA
Cuts at: 2452 5588 2452
Size: 3136 3648
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6784
BstYI r'GATC_y
Cuts at: 1972 2113 2764 4114 4481 4866 4883 6335 6421
Size: 141 651 1350 367 385 17 1452 86
Cuts at: 6421 6433 6779 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1350 651 385 367 346 141
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3662 3662
Size: 6784
BtgI C'CryG_G
Cuts at: 513 1065 2109 513
Size: 552 1044 5188
Fragments arranged by size:
5188 1044 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2829 487
Size: 683 56 1603 4442
Fragments arranged by size:
4442 1603 683 56
BtsI GCAGTG_nn'
Cuts at: 2825 3054 3115 3649 3903 4300 5094 5114 2825
Size: 229 61 534 254 397 794 20 4495
Fragments arranged by size:
4495 794 534 397 254 229 61 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6784
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6749
DraI TTT'AAA
Cuts at: 4088 4088
Size: 6784
DraIII CAC_nnn'GTG
Cuts at: 3596 3596
Size: 6784
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5791 809
Size: 507 4475 1802
Fragments arranged by size:
4475 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4231 5120 6749
Size: 54 1425 50 275 18 2401 889 1629
Cuts at: 6749 8
Size: 43
Fragments arranged by size:
2401 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4231 1537
Size: 275 2419 4090
Fragments arranged by size:
4090 2419 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2022 2781 4714 1353
Size: 669 759 1933 3423
Fragments arranged by size:
3423 1933 759 669
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5371 5745 5891 387
Size: 1064 138 3782 374 146 1280
Fragments arranged by size:
3782 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2220 4847 5444 6231 6499 1239
Size: 393 588 2627 597 787 268 1524
Fragments arranged by size:
2627 1524 787 597 588 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3866 3866
Size: 6784
EcoRI G'AATT_C
Cuts at: 4093 4471 4093
Size: 378 6406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2153 2279 2807 2153
Size: 126 528 6130
Fragments arranged by size:
6130 528 126
FspI TGC'GCA
Cuts at: 5290 5290
Size: 6784
HaeII r_GCGC'y
Cuts at: 1678 2621 5931 1678
Size: 943 3310 2531
Fragments arranged by size:
3310 2531 943
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 3503 3535 3785
Size: 32 135 32 427 32 1472 32 250
Cuts at: 3785 3817 5425 5457 5499 5531 1373
Size: 32 1608 32 42 32 2626
Fragments arranged by size:
2626 1608 1472 427 250 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4128 4374 669
Size: 988 281 2190 246 3079
Fragments arranged by size:
3079 2190 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2447 2500 748
Size: 1699 53 5032
Fragments arranged by size:
5032 1699 53
HpaI GTT'AAC
Cuts at: 4374 4374
Size: 6784
KpnI G_GTAC'C
Cuts at: 4109 4109
Size: 6784
MfeI C'AATT_G
Cuts at: 4383 4383
Size: 6784
MluI A'CGCG_T
Cuts at: 6660 6660
Size: 6784
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2179 2316 4039 5898 6082 1305
Size: 874 137 1723 1859 184 2007
Fragments arranged by size:
2007 1859 1723 874 184 137
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4962
Fragments arranged by size:
4962 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3933 4742 5101 5260 518
Size: 648 2767 809 359 159 2042
Fragments arranged by size:
2767 2042 809 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2846 4200 4877 6025 6270 1606
Size: 1240 1354 677 1148 245 2120
Fragments arranged by size:
2120 1354 1240 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2109 513
Size: 552 1044 5188
Fragments arranged by size:
5188 1044 552
NdeI CA'TA_TG
Cuts at: 387 4005 387
Size: 3618 3166
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6784
NotI GC'GGCC_GC
Cuts at: 4231 4231
Size: 6784
NspI r_CATG'y
Cuts at: 1132 1229 2477 3137 4142 5687 1132
Size: 97 1248 660 1005 1545 2229
Fragments arranged by size:
2229 1545 1248 1005 660 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6784
PciI A'CATG_T
Cuts at: 5683 5683
Size: 6784
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6784
PmeI GTTT'AAAC
Cuts at: 4088 4088
Size: 6784
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3306 3338 4853 4885 6366 6398 3306
Size: 32 1515 32 1481 32 3692
Fragments arranged by size:
3692 1515 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6784
PsiI TTA'TAA
Cuts at: 2037 4354 2037
Size: 2317 4467
PstI C_TGCA'G
Cuts at: 830 4136 830
Size: 3306 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6784
PvuII CAG'CTG
Cuts at: 2846 2846
Size: 6784
SalI G'TCGA_C
Cuts at: 1936 4126 1936
Size: 2190 4594
SapI GCTCTTCn'nnn_
Cuts at: 2781 2781
Size: 6784
SbfI CC_TGCA'GG
Cuts at: 4136 4136
Size: 6784
ScaI AGT'ACT
Cuts at: 2743 5032 6744 2743
Size: 2289 1712 2783
Fragments arranged by size:
2783 2289 1712
SfcI C'TryA_G
Cuts at: 826 1045 2583 3558 3621 4132 5267 5948 6139
Size: 219 1538 975 63 511 1135 681 191
Cuts at: 6139 826
Size: 1471
Fragments arranged by size:
1538 1471 1135 975 681 511 219 191
63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6784
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6784
SmaI CCC'GGG
Cuts at: 1872 4111 1872
Size: 2239 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3312 3810 4888 5789 6051 820
Size: 197 926 1369 498 1078 901 262 1553
Fragments arranged by size:
1553 1369 1078 926 901 498 262 197
SnaBI TAC'GTA
Cuts at: 493 4575 493
Size: 4082 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6784
SphI G_CATG'C
Cuts at: 1132 4142 1132
Size: 3010 3774
SspI AAT'ATT
Cuts at: 5 52 2205 2964 4027 4039 4708 5
Size: 47 2153 759 1063 12 669 2081
Fragments arranged by size:
2153 2081 1063 759 669 47 12
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6784
StyI C'CwwG_G
Cuts at: 513 1065 2109 4223 513
Size: 552 1044 2114 3074
Fragments arranged by size:
3074 2114 1044 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3251 4811 4828 4981 5166 5611 325
Size: 1255 1671 1560 17 153 185 445 1498
Fragments arranged by size:
1671 1560 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2725 2741 3190
Size: 274 80 33 51 1443 748 16 449
Cuts at: 3190 3904 5030 6742 96
Size: 714 1126 1712 138
Fragments arranged by size:
1712 1443 1126 748 714 449 274 138
80 51 33 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2850 2882 3474 3506 6744 6776 2850
Size: 32 592 32 3238 32 2858
Fragments arranged by size:
3238 2858 592 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6784
XbaI T'CTAG_A
Cuts at: 4120 4120
Size: 6784
XhoI C'TCGA_G
Cuts at: 1943 3312 1943
Size: 1369 5415
XmaI C'CCGG_G
Cuts at: 1870 4109 1870
Size: 2239 4545
XmnI GAAnn'nnTTC
Cuts at: 4913 4913
Size: 6784
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4592 276
Size: 53 83 186 936 3058 2468
Fragments arranged by size:
3058 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI