Restriction map for
pFN21AE4191
(Circular) (Six-base) MAPSORT of: pFN21AE4191.seq Check: 4842 from: 1 to: 6769
pFN21AE4191 6769 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4579 278
Size: 53 83 186 936 3043 2468
Fragments arranged by size:
3043 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4112 1937
Size: 2175 4594
Acc65I G'GTAC_C
Cuts at: 2721 4090 2721
Size: 1369 5400
AclI AA'CG_TT
Cuts at: 1563 4896 5269 1563
Size: 3333 373 3063
Fragments arranged by size:
3333 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4832 6216 6484 1632
Size: 3200 1384 268 1917
Fragments arranged by size:
3200 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6572
AflIII A'CryG_T
Cuts at: 1203 2196 2448 2619 2733 5668 6645 1203
Size: 993 252 171 114 2935 977 1327
Fragments arranged by size:
2935 1327 993 977 252 171 114
AgeI A'CCGG_T
Cuts at: 5600 5600
Size: 6769
AhdI GACnn_n'nnGTC
Cuts at: 5498 5498
Size: 6769
AleI CACnn'nnGTG
Cuts at: 1166 2519 1166
Size: 1353 5416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6769
AlwNI CAG_nnn'CTG
Cuts at: 3203 3368 4512 6084 3203
Size: 165 1144 1572 3888
Fragments arranged by size:
3888 1572 1144 165
ApaI G_GGCC'C
Cuts at: 2824 2899 2824
Size: 75 6694
ApaLI G'TGCA_C
Cuts at: 4826 5982 4826
Size: 1156 5613
ApoI r'AATT_y
Cuts at: 1493 2849 4078 4304 4456 6565 1493
Size: 1356 1229 226 152 2109 1697
Fragments arranged by size:
2109 1697 1356 1229 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5323 160
Size: 872 4291 1606
Fragments arranged by size:
4291 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6769
AvaI C'yCGr_G
Cuts at: 1870 1943 2654 4094 1870
Size: 73 711 1440 4545
Fragments arranged by size:
4545 1440 711 73
BamHI G'GATC_C
Cuts at: 3743 4099 4466 3743
Size: 356 367 6046
Fragments arranged by size:
6046 367 356
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2721 4090 5545 618
Size: 325 217 627 934 1369 1455 1842
Fragments arranged by size:
1842 1455 1369 934 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2071 2697 2824 2837 2899 3560 4000 4088
Size: 680 626 127 13 62 661 440 88
Cuts at: 4088 1391
Size: 4072
Fragments arranged by size:
4072 680 661 626 440 127 88 62
13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2412 928
Size: 971 513 5285
Fragments arranged by size:
5285 971 513
BbvCI CC'TCA_GC
Cuts at: 2088 2321 3416 4050 2088
Size: 233 1095 634 4807
Fragments arranged by size:
4807 1095 634 233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4960 4994 1903
Size: 34 3023 34 3678
Fragments arranged by size:
3678 3023 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2748 4661 5871 2748
Size: 1913 1210 3646
Fragments arranged by size:
3646 1913 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6769
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2021 5380 136
Size: 107 122 71 631 605 349 3359 1525
Fragments arranged by size:
3359 1525 631 605 349 122 107 71
BglII A'GATC_T
Cuts at: 6764 6764
Size: 6769
BlpI GC'TnA_GC
Cuts at: 2555 4186 2555
Size: 1631 5138
Bme1580I G_kGCm'C
Cuts at: 1790 2824 2899 4830 5986 1790
Size: 1034 75 1931 1156 2573
Fragments arranged by size:
2573 1931 1156 1034 75
BmgBI CAC'GTC
Cuts at: 1318 2197 2449 1318
Size: 879 252 5638
Fragments arranged by size:
5638 879 252
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2323 2419 4500
Size: 416 53 83 399 24 906 96 2081
Cuts at: 4500 5458 442
Size: 958 1753
Fragments arranged by size:
2081 1753 958 906 416 399 96 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6769
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 3588 3784 2176
Size: 1412 196 5161
Fragments arranged by size:
5161 1412 196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3518 3544 3905 5429 1239
Size: 2279 26 361 1524 2579
Fragments arranged by size:
2579 2279 1524 361 26
Bpu10I CC'TnA_GC
Cuts at: 2070 2088 2321 2600 3416 4050 2070
Size: 18 233 279 816 634 4789
Fragments arranged by size:
4789 816 634 279 233 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2792 2855 3230 3305 4894 5759 6057 2792
Size: 63 375 75 1589 865 298 3504
Fragments arranged by size:
3504 1589 865 375 298 75 63
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5432 882
Size: 617 3933 2219
Fragments arranged by size:
3933 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2622 4560 493
Size: 711 1418 1938 2702
Fragments arranged by size:
2702 1938 1418 711
BsaBI GATnn'nnATC
Cuts at: 6763 6763
Size: 6769
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3502 4576 4958 5593
Size: 53 83 186 936 1969 1074 382 635
Cuts at: 5593 275
Size: 1451
Fragments arranged by size:
1969 1451 1074 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2042 5202 5600 5874 6021 6432 2042
Size: 3160 398 274 147 411 2379
Fragments arranged by size:
3160 2379 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3542 3572 1178
Size: 30 165 30 2139 30 4375
Fragments arranged by size:
4375 2139 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2108 2111 2672 3447 3649 4055 1186
Size: 919 3 3 561 775 202 406 3900
Fragments arranged by size:
3900 919 775 561 406 202 3 3
BseYI C'CCAG_C
Cuts at: 2823 3350 3592 5972 6468 2823
Size: 527 242 2380 496 3124
Fragments arranged by size:
3124 2380 527 496 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2930 1163
Size: 573 1194 5002
Fragments arranged by size:
5002 1194 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2085 3115 4219 4980 6008 1540
Size: 275 186 84 1030 1104 761 1028 2301
Fragments arranged by size:
2301 1104 1030 1028 761 275 186 84
BsiHKAI G_wGCw'C
Cuts at: 2071 3891 4830 4915 5986 2071
Size: 1820 939 85 1071 2854
Fragments arranged by size:
2854 1820 1071 939 85
BsmI GAATG_Cn'
Cuts at: 4280 4373 4280
Size: 93 6676
BsmBI CGTCTCn'nnnn_
Cuts at: 3509 3509
Size: 6769
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2697 2824 2837 2899 3560 3891
Size: 399 281 626 127 13 62 661 331
Cuts at: 3891 4000 4088 4830 4915 5986 1391
Size: 109 88 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 661 626 399 331 281
127 109 88 85 62 13
BspEI T'CCGG_A
Cuts at: 2042 6432 2042
Size: 4390 2379
BspHI T'CATG_A
Cuts at: 3078 4658 6388 3078
Size: 1580 1730 3459
Fragments arranged by size:
3459 1730 1580
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2364 2733 2961 3195 4023 4124 844
Size: 1520 369 228 234 828 101 3489
Fragments arranged by size:
3489 1520 828 369 234 228 101
BsrBI CCG'CTC
Cuts at: 1394 1823 2160 2764 4656 1394
Size: 429 337 604 1892 3507
Fragments arranged by size:
3507 1892 604 429 337
BsrDI GCAATG_nn'
Cuts at: 65 1248 2396 3916 5264 5438 65
Size: 1183 1148 1520 1348 174 1396
Fragments arranged by size:
1520 1396 1348 1183 1148 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2274 5413 5600 1700
Size: 110 464 3139 187 2869
Fragments arranged by size:
3139 2869 464 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6769
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6769
BssSI C'ACGA_G
Cuts at: 2633 4829 5841 2633
Size: 2196 1012 3561
Fragments arranged by size:
3561 2196 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2953 794
Size: 2159 4610
BstBI TT'CG_AA
Cuts at: 5573 5573
Size: 6769
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6769
BstXI CCAn_nnnn'nTGG
Cuts at: 3358 3836 3883 3358
Size: 478 47 6244
Fragments arranged by size:
6244 478 47
BstYI r'GATC_y
Cuts at: 1972 2173 2673 3743 4099 4466 4851 4868 6320
Size: 201 500 1070 356 367 385 17 1452
Cuts at: 6320 6406 6418 6764 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1070 500 385 367 356 346
201 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2838 2919 2838
Size: 81 6688
BtgI C'CryG_G
Cuts at: 513 1065 2004 3120 513
Size: 552 939 1116 4162
Fragments arranged by size:
4162 1116 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2619 487
Size: 683 56 1393 4637
Fragments arranged by size:
4637 1393 683 56
BtsI GCAGTG_nn'
Cuts at: 2337 3650 4285 5079 5099 2337
Size: 1313 635 794 20 4007
Fragments arranged by size:
4007 1313 794 635 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6769
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3115 3150 529
Size: 35 2551 35 4148
Fragments arranged by size:
4148 2551 35 35
DraI TTT'AAA
Cuts at: 4073 4073
Size: 6769
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3575 5776 809
Size: 507 2259 2201 1802
Fragments arranged by size:
2259 2201 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 3090 3112
Size: 54 1425 50 275 18 252 1008 22
Cuts at: 3112 3123 3379 3468 3838 3873 3933 4216 5105
Size: 11 256 89 370 35 60 283 889
Cuts at: 5105 6734 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1008 889 370 283 275 256
252 89 60 54 50 43 35 22
18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2082 3112 4216 1537
Size: 275 270 1030 1104 4090
Fragments arranged by size:
4090 1104 1030 275 270
EarI CTCTTCn'nnn_
Cuts at: 1353 3218 4699 1353
Size: 1865 1481 3423
Fragments arranged by size:
3423 1865 1481
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5356 5730 5876 387
Size: 1064 138 3767 374 146 1280
Fragments arranged by size:
3767 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2069 2069
Size: 6769
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3518 3544 3905 4832 5429 6216 6484
Size: 393 1886 26 361 927 597 787 268
Cuts at: 6484 1239
Size: 1524
Fragments arranged by size:
1886 1524 927 787 597 393 361 268
26
EcoNI CCTnn'n_nnAGG
Cuts at: 2516 3191 3512 2516
Size: 675 321 5773
Fragments arranged by size:
5773 675 321
EcoO109I rG'GnC_Cy
Cuts at: 2022 2317 2842 2915 3485 3568 2022
Size: 295 525 73 570 83 5223
Fragments arranged by size:
5223 570 525 295 83 73
EcoRI G'AATT_C
Cuts at: 2849 4078 4456 2849
Size: 1229 378 5162
Fragments arranged by size:
5162 1229 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 6769
FspI TGC'GCA
Cuts at: 2885 3908 5275 2885
Size: 1023 1367 4379
Fragments arranged by size:
4379 1367 1023
HaeII r_GCGC'y
Cuts at: 1678 2051 2945 3204 5916 1678
Size: 373 894 259 2712 2531
Fragments arranged by size:
2712 2531 894 373 259
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 3002 3034 3668
Size: 32 135 32 881 32 517 32 634
Cuts at: 3668 3700 5410 5442 5484 5516 1373
Size: 32 1710 32 42 32 2626
Fragments arranged by size:
2626 1710 881 634 517 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3460 4113 4359 669
Size: 988 281 1522 653 246 3079
Fragments arranged by size:
3079 1522 988 653 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6769
HpaI GTT'AAC
Cuts at: 4359 4359
Size: 6769
KpnI G_GTAC'C
Cuts at: 2725 4094 2725
Size: 1369 5400
MfeI C'AATT_G
Cuts at: 2360 4368 2360
Size: 2008 4761
MluI A'CGCG_T
Cuts at: 6645 6645
Size: 6769
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2469 2569 3322 5883 6067 1305
Size: 1164 100 753 2561 184 2007
Fragments arranged by size:
2561 2007 1164 753 184 100
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3125 3381 3470 3875 3935
Size: 54 1425 343 1293 256 89 405 60
Cuts at: 3935 10
Size: 2844
Fragments arranged by size:
2844 1425 1293 405 343 256 89 60
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2519 2685 4727 5086 5245 518
Size: 648 1353 166 2042 359 159 2042
Fragments arranged by size:
2042 2042 1353 648 359 166 159
MspA1I CmG'CkG
Cuts at: 1606 2087 3228 3583 3596 4185 4862 6010 6255
Size: 481 1141 355 13 589 677 1148 245
Cuts at: 6255 1606
Size: 2120
Fragments arranged by size:
2120 1148 1141 677 589 481 355 245
13
NcoI C'CATG_G
Cuts at: 513 1065 2004 3120 513
Size: 552 939 1116 4162
Fragments arranged by size:
4162 1116 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6769
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6769
NotI GC'GGCC_GC
Cuts at: 2082 4216 2082
Size: 2134 4635
NspI r_CATG'y
Cuts at: 1132 1229 3283 3415 4127 5672 1132
Size: 97 2054 132 712 1545 2229
Fragments arranged by size:
2229 2054 1545 712 132 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6769
PciI A'CATG_T
Cuts at: 5668 5668
Size: 6769
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6769
PfoI T'CCnGG_A
Cuts at: 3560 3560
Size: 6769
PmeI GTTT'AAAC
Cuts at: 4073 4073
Size: 6769
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4838 4870 6351 6383 4838
Size: 32 1481 32 5224
Fragments arranged by size:
5224 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2317 2842 3568 2317
Size: 525 726 5518
Fragments arranged by size:
5518 726 525
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6769
PsiI TTA'TAA
Cuts at: 3924 4339 3924
Size: 415 6354
PspOMI G'GGCC_C
Cuts at: 2820 2895 2820
Size: 75 6694
PstI C_TGCA'G
Cuts at: 830 4020 4121 830
Size: 3190 101 3478
Fragments arranged by size:
3478 3190 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6769
PvuII CAG'CTG
Cuts at: 3583 3596 3583
Size: 13 6756
SacI G_AGCT'C
Cuts at: 2071 2071
Size: 6769
SalI G'TCGA_C
Cuts at: 1936 4111 1936
Size: 2175 4594
SanDI GG'GwC_CC
Cuts at: 3568 3568
Size: 6769
SbfI CC_TGCA'GG
Cuts at: 4121 4121
Size: 6769
ScaI AGT'ACT
Cuts at: 5017 6729 5017
Size: 1712 5057
SfcI C'TryA_G
Cuts at: 826 1045 2169 3738 4016 4117 5252 5933 6124
Size: 219 1124 1569 278 101 1135 681 191
Cuts at: 6124 826
Size: 1471
Fragments arranged by size:
1569 1471 1135 1124 681 278 219 191
101
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6769
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6769
SmaI CCC'GGG
Cuts at: 1872 4096 1872
Size: 2224 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2807 2870 3245 3284 4873 5774
Size: 197 926 864 63 375 39 1589 901
Cuts at: 5774 6036 820
Size: 262 1553
Fragments arranged by size:
1589 1553 926 901 864 375 262 197
63 39
SnaBI TAC'GTA
Cuts at: 493 4560 493
Size: 4067 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6769
SphI G_CATG'C
Cuts at: 1132 3283 4127 1132
Size: 2151 844 3774
Fragments arranged by size:
3774 2151 844
SspI AAT'ATT
Cuts at: 5 52 4693 5
Size: 47 4641 2081
Fragments arranged by size:
4641 2081 47
StuI AGG'CCT
Cuts at: 1517 3421 3716 1517
Size: 1904 295 4570
Fragments arranged by size:
4570 1904 295
StyI C'CwwG_G
Cuts at: 513 1065 2004 3120 4208 513
Size: 552 939 1116 1088 3074
Fragments arranged by size:
3074 1116 1088 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2994 3037 3606 3722 4796 4813 4966
Size: 1255 1414 43 569 116 1074 17 153
Cuts at: 4966 5151 5596 325
Size: 185 445 1498
Fragments arranged by size:
1498 1414 1255 1074 569 445 185 153
116 43 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2878 5015 6727
Size: 274 80 33 51 1443 901 2137 1712
Cuts at: 6727 96
Size: 138
Fragments arranged by size:
2137 1712 1443 901 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2493 2525 3662 3694 6729 6761 2493
Size: 32 1137 32 3035 32 2501
Fragments arranged by size:
3035 2501 1137 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6769
XbaI T'CTAG_A
Cuts at: 4105 4105
Size: 6769
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3730 3730
Size: 6769
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6769
XmaI C'CCGG_G
Cuts at: 1870 4094 1870
Size: 2224 4545
XmnI GAAnn'nnTTC
Cuts at: 3851 4898 3851
Size: 1047 5722
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4577 276
Size: 53 83 186 936 3043 2468
Fragments arranged by size:
3043 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI