Restriction map for
pFN21AE4183
(Circular) (Six-base) MAPSORT of: pFN21AE4183.seq Check: 3934 from: 1 to: 6670
pFN21AE4183 6670 bp
With 182 enzymes: SgfI *
March 28, 2012 13:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3447 3647 3681 3447
Size: 200 34 6436
Fragments arranged by size:
6436 200 34
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4480 278
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4013 1937
Size: 2076 4594
Acc65I G'GTAC_C
Cuts at: 3991 3991
Size: 6670
AclI AA'CG_TT
Cuts at: 1563 4797 5170 1563
Size: 3234 373 3063
Fragments arranged by size:
3234 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2872 2966 4733 6117 6385 1632
Size: 1240 94 1767 1384 268 1917
Fragments arranged by size:
1917 1767 1384 1240 268 94
AfeI AGC'GCT
Cuts at: 2183 2666 3870 2183
Size: 483 1204 4983
Fragments arranged by size:
4983 1204 483
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6473
AflIII A'CryG_T
Cuts at: 1203 2847 5569 6546 1203
Size: 1644 2722 977 1327
Fragments arranged by size:
2722 1644 1327 977
AgeI A'CCGG_T
Cuts at: 5501 5501
Size: 6670
AhdI GACnn_n'nnGTC
Cuts at: 5399 5399
Size: 6670
AleI CACnn'nnGTG
Cuts at: 1166 2603 1166
Size: 1437 5233
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2666 1184
Size: 1482 5188
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2204 2236 3575 3607 3917 3949 2204
Size: 32 1339 32 310 32 4925
Fragments arranged by size:
4925 1339 310 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2923 3203 4413 5985 2923
Size: 280 1210 1572 3608
Fragments arranged by size:
3608 1572 1210 280
ApaLI G'TGCA_C
Cuts at: 4727 5883 4727
Size: 1156 5514
ApoI r'AATT_y
Cuts at: 1493 3979 4205 4357 6466 1493
Size: 2486 226 152 2109 1697
Fragments arranged by size:
2486 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5224 160
Size: 872 4192 1606
Fragments arranged by size:
4192 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
AvaI C'yCGr_G
Cuts at: 1870 1943 2040 2267 2635 3995 1870
Size: 73 97 227 368 1360 4545
Fragments arranged by size:
4545 1360 368 227 97 73
BamHI G'GATC_C
Cuts at: 2928 3291 4000 4367 2928
Size: 363 709 367 5231
Fragments arranged by size:
5231 709 367 363
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2617 2855 3124 3377 3991
Size: 325 217 627 830 238 269 253 614
Cuts at: 3991 5446 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 830 627 614 325 269 253
238 217
BanII G_rGCy'C
Cuts at: 1391 3041 3351 3723 3989 1391
Size: 1650 310 372 266 4072
Fragments arranged by size:
4072 1650 372 310 266
BbeI G_GCGC'C
Cuts at: 2859 2859
Size: 6670
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2548 2869 2878 3668 928
Size: 971 649 321 9 790 3930
Fragments arranged by size:
3930 971 790 649 321 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4861 4895 1903
Size: 34 2924 34 3678
Fragments arranged by size:
3678 2924 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3104 4562 5772 3104
Size: 1458 1210 4002
Fragments arranged by size:
4002 1458 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6670
BfrBI ATG'CAT
Cuts at: 3323 3323
Size: 6670
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3624 5281
Size: 107 122 71 631 605 337 1615 1657
Cuts at: 5281 136
Size: 1525
Fragments arranged by size:
1657 1615 1525 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 3147 6665 3147
Size: 3518 3152
BlpI GC'TnA_GC
Cuts at: 2492 3722 4087 2492
Size: 1230 365 5075
Fragments arranged by size:
5075 1230 365
Bme1580I G_kGCm'C
Cuts at: 1790 3268 3380 4731 5887 1790
Size: 1478 112 1351 1156 2573
Fragments arranged by size:
2573 1478 1351 1156 112
BmgBI CAC'GTC
Cuts at: 1318 2794 2848 1318
Size: 1476 54 5140
Fragments arranged by size:
5140 1476 54
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2975 3116 3474
Size: 416 53 83 399 24 1558 141 358
Cuts at: 3474 4401 5359 442
Size: 927 958 1753
Fragments arranged by size:
1753 1558 958 927 416 399 358 141
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6670
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2686 3055 3772 2353
Size: 333 369 717 5251
Fragments arranged by size:
5251 717 369 333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2185 2198 3386 3760 5330 1239
Size: 946 13 1188 374 1570 2579
Fragments arranged by size:
2579 1570 1188 946 374 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3190 4795 5660 5958 3190
Size: 1605 865 298 3902
Fragments arranged by size:
3902 1605 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3802 3964 5333 882
Size: 617 2303 162 1369 2219
Fragments arranged by size:
2303 2219 1369 617 162
BsaAI yAC'GTr
Cuts at: 493 1204 2566 4461 493
Size: 711 1362 1895 2702
Fragments arranged by size:
2702 1895 1362 711
BsaBI GATnn'nnATC
Cuts at: 3734 6664 3734
Size: 2930 3740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2856 3177 4477 4859
Size: 53 83 186 936 1323 321 1300 382
Cuts at: 4859 5494 275
Size: 635 1451
Fragments arranged by size:
1451 1323 1300 936 635 382 321 186
83 53
BsaWI w'CCGG_w
Cuts at: 5103 5501 5775 5922 6333 5103
Size: 398 274 147 411 5440
Fragments arranged by size:
5440 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 2639 2669 3108
Size: 30 165 30 801 30 405 30 439
Cuts at: 3108 3138 1178
Size: 30 4710
Fragments arranged by size:
4710 801 439 405 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 3031 3341 3404 1186
Size: 1234 611 310 63 4452
Fragments arranged by size:
4452 1234 611 310 63
BseYI C'CCAG_C
Cuts at: 2200 2750 3865 5873 6369 2200
Size: 550 1115 2008 496 2501
Fragments arranged by size:
2501 2008 1115 550 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 3314 3428 1163
Size: 573 561 1017 114 4405
Fragments arranged by size:
4405 1017 573 561 114
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 2100 2260 4120 4881 5909
Size: 275 186 12 87 160 1860 761 1028
Cuts at: 5909 1540
Size: 2301
Fragments arranged by size:
2301 1860 1028 761 275 186 160 87
12
BsiHKAI G_wGCw'C
Cuts at: 2289 3396 3723 4731 4816 5887 2289
Size: 1107 327 1008 85 1071 3072
Fragments arranged by size:
3072 1107 1071 1008 327 85
BsmI GAATG_Cn'
Cuts at: 3325 4181 4274 3325
Size: 856 93 5721
Fragments arranged by size:
5721 856 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2606 2606
Size: 6670
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2289 3041 3268 3351 3380 3396 3723
Size: 399 499 752 227 83 29 16 327
Cuts at: 3723 3989 4731 4816 5887 1391
Size: 266 742 85 1071 2174
Fragments arranged by size:
2174 1071 752 742 499 399 327 266
227 85 83 29 16
BspEI T'CCGG_A
Cuts at: 6333 6333
Size: 6670
BspHI T'CATG_A
Cuts at: 2706 2778 2829 4559 6289 2706
Size: 72 51 1730 1730 3087
Fragments arranged by size:
3087 1730 1730 72 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3336 3447 3647 3681 3696 4025 844
Size: 2492 111 200 34 15 329 3489
Fragments arranged by size:
3489 2492 329 200 111 34 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2440 4557 1394
Size: 429 617 2117 3507
Fragments arranged by size:
3507 2117 617 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3186 3952 5165 5339 65
Size: 1183 1938 766 1213 174 1396
Fragments arranged by size:
1938 1396 1213 1183 766 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 5314 5501 1700
Size: 110 255 3249 187 2869
Fragments arranged by size:
3249 2869 255 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6670
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6670
BssSI C'ACGA_G
Cuts at: 3527 4730 5742 3527
Size: 1203 1012 4455
Fragments arranged by size:
4455 1203 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6670
BstBI TT'CG_AA
Cuts at: 3317 5474 3317
Size: 2157 4513
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6670
BstXI CCAn_nnnn'nTGG
Cuts at: 2089 2545 2608 2089
Size: 456 63 6151
Fragments arranged by size:
6151 456 63
BstYI r'GATC_y
Cuts at: 1972 2892 2928 3147 3291 4000 4367 4752 4769
Size: 920 36 219 144 709 367 385 17
Cuts at: 4769 6221 6307 6319 6665 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 920 709 385 367 346 219
144 86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2718 2991 513
Size: 552 939 714 273 4192
Fragments arranged by size:
4192 939 714 552 273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2566 487
Size: 683 56 1340 4591
Fragments arranged by size:
4591 1340 683 56
BtsI GCAGTG_nn'
Cuts at: 3445 3751 4186 4980 5000 3445
Size: 306 435 794 20 5115
Fragments arranged by size:
5115 794 435 306 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6670
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2125 2160 529
Size: 35 1561 35 5039
Fragments arranged by size:
5039 1561 35 35
DraI TTT'AAA
Cuts at: 3974 3974
Size: 6670
DraIII CAC_nnn'GTG
Cuts at: 2700 2700
Size: 6670
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5677 809
Size: 507 4361 1802
Fragments arranged by size:
4361 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2049 2070
Size: 54 1425 50 275 18 180 39 21
Cuts at: 2070 2097 2799 2994 3045 3300 3893 4117 5006
Size: 27 702 195 51 255 593 224 889
Cuts at: 5006 6635 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 702 593 275 255 224
195 180 54 51 50 43 39 27
21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 2097 4117 1537
Size: 275 198 87 2020 4090
Fragments arranged by size:
4090 2020 275 198 87
EarI CTCTTCn'nnn_
Cuts at: 1353 2744 2942 2984 3381 4600 1353
Size: 1391 198 42 397 1219 3423
Fragments arranged by size:
3423 1391 1219 397 198 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2164 2489 3952 5257 5631 5777
Size: 1064 138 575 325 1463 1305 374 146
Cuts at: 5777 387
Size: 1280
Fragments arranged by size:
1463 1305 1280 1064 575 374 325 146
138
EcoICRI GAG'CTC
Cuts at: 3721 3721
Size: 6670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2185 2198 2872 2966 3386 3760 4733
Size: 393 553 13 674 94 420 374 973
Cuts at: 4733 5330 6117 6385 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 973 787 674 597 553 420 393
374 268 94 13
EcoNI CCTnn'n_nnAGG
Cuts at: 3356 3356
Size: 6670
EcoO109I rG'GnC_Cy
Cuts at: 2263 2920 3914 2263
Size: 657 994 5019
Fragments arranged by size:
5019 994 657
EcoRI G'AATT_C
Cuts at: 3979 4357 3979
Size: 378 6292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2239 2266 2239
Size: 27 6643
FspI TGC'GCA
Cuts at: 3707 5176 3707
Size: 1469 5201
HaeII r_GCGC'y
Cuts at: 1678 2185 2668 2859 3365 3872 5817 1678
Size: 507 483 191 506 507 1945 2531
Fragments arranged by size:
2531 1945 507 507 506 483 191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2476 2834 2866 3415
Size: 32 135 32 872 32 358 32 549
Cuts at: 3415 3447 5311 5343 5385 5417 1373
Size: 32 1864 32 42 32 2626
Fragments arranged by size:
2626 1864 872 549 358 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4014 4260 669
Size: 988 281 2076 246 3079
Fragments arranged by size:
3079 2076 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6670
HpaI GTT'AAC
Cuts at: 4260 4260
Size: 6670
KasI G'GCGC_C
Cuts at: 2855 2855
Size: 6670
KpnI G_GTAC'C
Cuts at: 3995 3995
Size: 6670
MfeI C'AATT_G
Cuts at: 4269 4269
Size: 6670
MluI A'CGCG_T
Cuts at: 6546 6546
Size: 6670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3240 5784 5968 1305
Size: 1935 2544 184 2007
Fragments arranged by size:
2544 2007 1935 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2072 2801 2996 3302 10
Size: 54 1425 343 240 729 195 306 3378
Fragments arranged by size:
3378 1425 729 343 306 240 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2603 2792 3017 4628 4987 5146 518
Size: 648 1437 189 225 1611 359 159 2042
Fragments arranged by size:
2042 1611 1437 648 359 225 189 159
MspA1I CmG'CkG
Cuts at: 1606 2280 4086 4763 5911 6156 1606
Size: 674 1806 677 1148 245 2120
Fragments arranged by size:
2120 1806 1148 677 674 245
NarI GG'CG_CC
Cuts at: 2856 2856
Size: 6670
NcoI C'CATG_G
Cuts at: 513 1065 2004 2991 513
Size: 552 939 987 4192
Fragments arranged by size:
4192 987 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6670
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6670
NotI GC'GGCC_GC
Cuts at: 4117 4117
Size: 6670
NsiI A_TGCA'T
Cuts at: 3325 3325
Size: 6670
NspI r_CATG'y
Cuts at: 1132 1229 3715 4028 5573 1132
Size: 97 2486 313 1545 2229
Fragments arranged by size:
2486 2229 1545 313 97
PasI CC'CwG_GG
Cuts at: 1348 2917 1348
Size: 1569 5101
PciI A'CATG_T
Cuts at: 5569 5569
Size: 6670
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2058 1108
Size: 950 5720
PfoI T'CCnGG_A
Cuts at: 2376 2376
Size: 6670
PmeI GTTT'AAAC
Cuts at: 3974 3974
Size: 6670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2204 2236 4739 4771 6252 6284 2204
Size: 32 2503 32 1481 32 2590
Fragments arranged by size:
2590 2503 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2263 2263
Size: 6670
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6670
PsiI TTA'TAA
Cuts at: 4240 4240
Size: 6670
PstI C_TGCA'G
Cuts at: 830 4022 830
Size: 3192 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6670
SacI G_AGCT'C
Cuts at: 3723 3723
Size: 6670
SalI G'TCGA_C
Cuts at: 1936 4012 1936
Size: 2076 4594
SanDI GG'GwC_CC
Cuts at: 2263 2263
Size: 6670
SapI GCTCTTCn'nnn_
Cuts at: 2744 2942 2744
Size: 198 6472
SbfI CC_TGCA'GG
Cuts at: 4022 4022
Size: 6670
ScaI AGT'ACT
Cuts at: 2249 4918 6630 2249
Size: 2669 1712 2289
Fragments arranged by size:
2669 2289 1712
SfcI C'TryA_G
Cuts at: 826 1045 4018 5153 5834 6025 826
Size: 219 2973 1135 681 191 1471
Fragments arranged by size:
2973 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2857 2857
Size: 6670
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6670
SmaI CCC'GGG
Cuts at: 1872 2042 2637 3997 1872
Size: 170 595 1360 4545
Fragments arranged by size:
4545 1360 595 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3169 4774 5675 5937 820
Size: 197 926 1226 1605 901 262 1553
Fragments arranged by size:
1605 1553 1226 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4461 493
Size: 3968 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6670
SphI G_CATG'C
Cuts at: 1132 3715 4028 1132
Size: 2583 313 3774
Fragments arranged by size:
3774 2583 313
SrfI GCCC'GGGC
Cuts at: 2042 2042
Size: 6670
SspI AAT'ATT
Cuts at: 5 52 4594 5
Size: 47 4542 2081
Fragments arranged by size:
4542 2081 47
StuI AGG'CCT
Cuts at: 1517 3627 1517
Size: 2110 4560
StyI C'CwwG_G
Cuts at: 513 1065 2004 2241 2991 3966 4109 513
Size: 552 939 237 750 975 143 3074
Fragments arranged by size:
3074 975 939 750 552 237 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2581 2810 3636 4697 4714 4867 5052
Size: 1255 1001 229 826 1061 17 153 185
Cuts at: 5052 5497 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1061 1001 826 445 229 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2247 2409 4916
Size: 274 80 33 51 1443 270 162 2507
Cuts at: 4916 6628 96
Size: 1712 138
Fragments arranged by size:
2507 1712 1443 274 270 162 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2639 2671 2684 2716 3108 3140 3586 3618 3913
Size: 32 13 32 392 32 446 32 295
Cuts at: 3913 3945 6630 6662 2639
Size: 32 2685 32 2647
Fragments arranged by size:
2685 2647 446 392 295 32 32 32
32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6670
XbaI T'CTAG_A
Cuts at: 4006 4006
Size: 6670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3103 3295 3103
Size: 192 6478
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6670
XmaI C'CCGG_G
Cuts at: 1870 2040 2635 3995 1870
Size: 170 595 1360 4545
Fragments arranged by size:
4545 1360 595 170
XmnI GAAnn'nnTTC
Cuts at: 2219 3149 4799 2219
Size: 930 1650 4090
Fragments arranged by size:
4090 1650 930
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4478 276
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstZ17I
Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI
SfiI SwaI