Restriction map for
pFN21AE4182
(Circular) (Six-base) MAPSORT of: pFN21AE4182.seq Check: 6464 from: 1 to: 6886
pFN21AE4182 6886 bp
With 182 enzymes: SgfI *
March 28, 2012 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2854 2854
Size: 6886
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4696 278
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2203 2436 3800 3878 4229 1937
Size: 266 233 1364 78 351 4594
Fragments arranged by size:
4594 1364 351 266 233 78
Acc65I G'GTAC_C
Cuts at: 4207 4207
Size: 6886
AclI AA'CG_TT
Cuts at: 1563 5013 5386 1563
Size: 3450 373 3063
Fragments arranged by size:
3450 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2705 2980 3141 3357 3761 3959 4949 6333
Size: 1073 275 161 216 404 198 990 1384
Cuts at: 6333 6601 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1073 990 404 275 268 216
198 161
AflII C'TTAA_G
Cuts at: 820 1017 2954 820
Size: 197 1937 4752
Fragments arranged by size:
4752 1937 197
AflIII A'CryG_T
Cuts at: 1203 2117 3758 5785 6762 1203
Size: 914 1641 2027 977 1327
Fragments arranged by size:
2027 1641 1327 977 914
AgeI A'CCGG_T
Cuts at: 5717 5717
Size: 6886
AhdI GACnn_n'nnGTC
Cuts at: 5615 5615
Size: 6886
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6886
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6886
AlwNI CAG_nnn'CTG
Cuts at: 3532 4629 6201 3532
Size: 1097 1572 4217
Fragments arranged by size:
4217 1572 1097
ApaLI G'TGCA_C
Cuts at: 4943 6099 4943
Size: 1156 5730
ApoI r'AATT_y
Cuts at: 1493 2172 2458 2653 2889 2948 4195 4421 4573
Size: 679 286 195 236 59 1247 226 152
Cuts at: 4573 6682 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1247 679 286 236 226 195
152 59
AseI AT'TA_AT
Cuts at: 160 1032 5440 160
Size: 872 4408 1606
Fragments arranged by size:
4408 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
AvaI C'yCGr_G
Cuts at: 1870 1943 3642 4211 1870
Size: 73 1699 569 4545
Fragments arranged by size:
4545 1699 569 73
AvrII C'CTAG_G
Cuts at: 3095 3095
Size: 6886
BamHI G'GATC_C
Cuts at: 4216 4583 4216
Size: 367 6519
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4207 5662 618
Size: 325 217 627 2420 1455 1842
Fragments arranged by size:
2420 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2094 3203 4205 1391
Size: 703 1109 1002 4072
Fragments arranged by size:
4072 1109 1002 703
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2408 928
Size: 971 509 5406
Fragments arranged by size:
5406 971 509
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3429 3463 5077 5111 1903
Size: 34 1492 34 1614 34 3678
Fragments arranged by size:
3678 1614 1492 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4778 5988 4778
Size: 1210 5676
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6886
BfrBI ATG'CAT
Cuts at: 2861 2861
Size: 6886
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3722 5497 136
Size: 107 122 71 631 605 2050 1775 1525
Fragments arranged by size:
2050 1775 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3614 6881 3614
Size: 3267 3619
BlpI GC'TnA_GC
Cuts at: 4303 4303
Size: 6886
Bme1580I G_kGCm'C
Cuts at: 1790 4947 6103 1790
Size: 3157 1156 2573
Fragments arranged by size:
3157 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6886
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3801 3844 4617
Size: 416 53 83 399 24 2384 43 773
Cuts at: 4617 5575 442
Size: 958 1753
Fragments arranged by size:
2384 1753 958 773 416 399 83 53
43 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6886
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 6886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2863 3296 3300 3794 5546 1239
Size: 1624 433 4 494 1752 2579
Fragments arranged by size:
2579 1752 1624 494 433 4
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3314 5011 5876 6174 3314
Size: 1697 865 298 4026
Fragments arranged by size:
4026 1697 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5549 882
Size: 617 4050 2219
Fragments arranged by size:
4050 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3761 4677 493
Size: 711 2557 916 2702
Fragments arranged by size:
2702 2557 916 711
BsaBI GATnn'nnATC
Cuts at: 2929 6880 2929
Size: 3951 2935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4693 5075 5710 275
Size: 53 83 186 936 3160 382 635 1451
Fragments arranged by size:
3160 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5319 5717 5991 6138 6549 5319
Size: 398 274 147 411 5656
Fragments arranged by size:
5656 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6661
Fragments arranged by size:
6661 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2754 3009 4144 1186
Size: 1568 255 1135 3928
Fragments arranged by size:
3928 1568 1135 255
BseYI C'CCAG_C
Cuts at: 2970 6089 6585 2970
Size: 3119 496 3271
Fragments arranged by size:
3271 3119 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2772 3423 4143 1163
Size: 573 1036 651 720 3906
Fragments arranged by size:
3906 1036 720 651 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4336 5097 6125 1540
Size: 275 186 2335 761 1028 2301
Fragments arranged by size:
2335 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2094 3734 3758 4947 5032 6103 2094
Size: 1640 24 1189 85 1071 2877
Fragments arranged by size:
2877 1640 1189 1071 85 24
BsmI GAATG_Cn'
Cuts at: 2863 4052 4397 4490 2863
Size: 1189 345 93 5259
Fragments arranged by size:
5259 1189 345 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 3203 3734 3758 4205 4947 5032
Size: 399 304 1109 531 24 447 742 85
Cuts at: 5032 6103 1391
Size: 1071 2174
Fragments arranged by size:
2174 1109 1071 742 531 447 399 304
85 24
BspEI T'CCGG_A
Cuts at: 6549 6549
Size: 6886
BspHI T'CATG_A
Cuts at: 4775 6505 4775
Size: 1730 5156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2854 4241 844
Size: 2010 1387 3489
Fragments arranged by size:
3489 2010 1387
BsrBI CCG'CTC
Cuts at: 1394 1823 4773 1394
Size: 429 2950 3507
Fragments arranged by size:
3507 2950 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5381 5555 65
Size: 1183 4133 174 1396
Fragments arranged by size:
4133 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5530 5717 1700
Size: 110 3720 187 2869
Fragments arranged by size:
3720 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6886
BssSI C'ACGA_G
Cuts at: 4142 4946 5958 4142
Size: 804 1012 5070
Fragments arranged by size:
5070 1012 804
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6886
BstBI TT'CG_AA
Cuts at: 2176 5690 2176
Size: 3514 3372
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6886
BstXI CCAn_nnnn'nTGG
Cuts at: 3890 3890
Size: 6886
BstYI r'GATC_y
Cuts at: 1972 3614 4216 4583 4968 4985 6437 6523 6535
Size: 1642 602 367 385 17 1452 86 12
Cuts at: 6535 6881 1972
Size: 346 1977
Fragments arranged by size:
1977 1642 1452 602 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2437 2437
Size: 6886
BtgI C'CryG_G
Cuts at: 513 1065 2004 2085 3883 513
Size: 552 939 81 1798 3516
Fragments arranged by size:
3516 1798 939 552 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6147
Fragments arranged by size:
6147 683 56
BtsI GCAGTG_nn'
Cuts at: 2784 4402 5196 5216 2784
Size: 1618 794 20 4454
Fragments arranged by size:
4454 1618 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6886
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4137 4172 529
Size: 35 3573 35 3243
Fragments arranged by size:
3573 3243 35 35
DraI TTT'AAA
Cuts at: 4190 4190
Size: 6886
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5893 809
Size: 507 4577 1802
Fragments arranged by size:
4577 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3723 4333 5222
Size: 54 1425 50 275 18 1893 610 889
Cuts at: 5222 6851 8
Size: 1629 43
Fragments arranged by size:
1893 1629 1425 889 610 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4333 1537
Size: 275 2521 4090
Fragments arranged by size:
4090 2521 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2099 2323 2518 3432 3978 4816 1353
Size: 746 224 195 914 546 838 3423
Fragments arranged by size:
3423 914 838 746 546 224 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2325 2856 5473 5847 5993 387
Size: 1064 138 736 531 2617 374 146 1280
Fragments arranged by size:
2617 1280 1064 736 531 374 146 138
EcoICRI GAG'CTC
Cuts at: 2092 2092
Size: 6886
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2705 2863 2980 3141 3296 3300 3357
Size: 393 1073 158 117 161 155 4 57
Cuts at: 3357 3761 3794 3959 4949 5546 6333 6601 1239
Size: 404 33 165 990 597 787 268 1524
Fragments arranged by size:
1524 1073 990 787 597 404 393 268
165 161 158 155 117 57 33 4
EcoO109I rG'GnC_Cy
Cuts at: 2815 2815
Size: 6886
EcoRI G'AATT_C
Cuts at: 4195 4573 4195
Size: 378 6508
EcoRV GAT'ATC
Cuts at: 3649 3649
Size: 6886
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3989 3989
Size: 6886
FspI TGC'GCA
Cuts at: 5392 5392
Size: 6886
HaeII r_GCGC'y
Cuts at: 1678 6033 1678
Size: 4355 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2376 2408 2731 2763 3467
Size: 32 135 32 804 32 323 32 704
Cuts at: 3467 3499 5527 5559 5601 5633 1373
Size: 32 2028 32 42 32 2626
Fragments arranged by size:
2626 2028 804 704 323 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2204 4230 4476 669
Size: 988 281 266 2026 246 3079
Fragments arranged by size:
3079 2026 988 281 266 246
HindIII A'AGCT_T
Cuts at: 748 4172 748
Size: 3424 3462
HpaI GTT'AAC
Cuts at: 4476 4476
Size: 6886
KpnI G_GTAC'C
Cuts at: 4211 4211
Size: 6886
MfeI C'AATT_G
Cuts at: 2453 4485 2453
Size: 2032 4854
MluI A'CGCG_T
Cuts at: 6762 6762
Size: 6886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3871 6000 6184 1305
Size: 2566 2129 184 2007
Fragments arranged by size:
2566 2129 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5064
Fragments arranged by size:
5064 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2076 2538 3656 4083 4844 5203 5362
Size: 648 910 462 1118 427 761 359 159
Cuts at: 5362 518
Size: 2042
Fragments arranged by size:
2042 1118 910 761 648 462 427 359
159
MspA1I CmG'CkG
Cuts at: 1606 3246 4302 4979 6127 6372 1606
Size: 1640 1056 677 1148 245 2120
Fragments arranged by size:
2120 1640 1148 1056 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2085 3883 513
Size: 552 939 81 1798 3516
Fragments arranged by size:
3516 1798 939 552 81
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6886
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6886
NotI GC'GGCC_GC
Cuts at: 4333 4333
Size: 6886
NsiI A_TGCA'T
Cuts at: 2863 2863
Size: 6886
NspI r_CATG'y
Cuts at: 1132 1229 2121 2543 3338 4244 5789 1132
Size: 97 892 422 795 906 1545 2229
Fragments arranged by size:
2229 1545 906 892 795 422 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6886
PciI A'CATG_T
Cuts at: 2117 5785 2117
Size: 3668 3218
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6886
PfoI T'CCnGG_A
Cuts at: 2879 2879
Size: 6886
PmeI GTTT'AAAC
Cuts at: 4190 4190
Size: 6886
PmlI CAC'GTG
Cuts at: 3761 3761
Size: 6886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2563 2595 4955 4987 6468 6500 2563
Size: 32 2360 32 1481 32 2949
Fragments arranged by size:
2949 2360 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2815 2815
Size: 6886
PshAI GACnn'nnGTC
Cuts at: 1613 3418 1613
Size: 1805 5081
PsiI TTA'TAA
Cuts at: 4456 4456
Size: 6886
PstI C_TGCA'G
Cuts at: 830 2414 2473 4238 830
Size: 1584 59 1765 3478
Fragments arranged by size:
3478 1765 1584 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6886
SacI G_AGCT'C
Cuts at: 2094 2094
Size: 6886
SalI G'TCGA_C
Cuts at: 1936 2202 4228 1936
Size: 266 2026 4594
Fragments arranged by size:
4594 2026 266
SapI GCTCTTCn'nnn_
Cuts at: 2099 2099
Size: 6886
SbfI CC_TGCA'GG
Cuts at: 4238 4238
Size: 6886
ScaI AGT'ACT
Cuts at: 2369 5134 6846 2369
Size: 2765 1712 2409
Fragments arranged by size:
2765 2409 1712
SfcI C'TryA_G
Cuts at: 826 1045 2062 2337 2410 2469 2757 3509 4234
Size: 219 1017 275 73 59 288 752 725
Cuts at: 4234 5369 6050 6241 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1017 752 725 681 288 275
219 191 73 59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6886
SmaI CCC'GGG
Cuts at: 1872 4213 1872
Size: 2341 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2954 3329 4990 5891 6153 820
Size: 197 926 1011 375 1661 901 262 1553
Fragments arranged by size:
1661 1553 1011 926 901 375 262 197
SnaBI TAC'GTA
Cuts at: 493 4677 493
Size: 4184 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6886
SphI G_CATG'C
Cuts at: 1132 4244 1132
Size: 3112 3774
SspI AAT'ATT
Cuts at: 5 52 4810 5
Size: 47 4758 2081
Fragments arranged by size:
4758 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6886
StyI C'CwwG_G
Cuts at: 513 1065 2004 2085 3095 3883 4325 513
Size: 552 939 81 1010 788 442 3074
Fragments arranged by size:
3074 1010 939 788 552 442 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2766 4913 4930 5083 5268 5713 325
Size: 1255 1186 2147 17 153 185 445 1498
Fragments arranged by size:
2147 1498 1255 1186 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2367 3938 5132
Size: 274 80 33 51 1443 390 1571 1194
Cuts at: 5132 6844 96
Size: 1712 138
Fragments arranged by size:
1712 1571 1443 1194 390 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6846 6878 6846
Size: 32 6854
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6886
XbaI T'CTAG_A
Cuts at: 4222 4222
Size: 6886
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6886
XmaI C'CCGG_G
Cuts at: 1870 4211 1870
Size: 2341 4545
XmnI GAAnn'nnTTC
Cuts at: 2190 5015 2190
Size: 2825 4061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4694 276
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI Bsu36I DraIII EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI