Restriction map for
pFN21AE4181
(Circular) (Six-base) MAPSORT of: pFN21AE4181.seq Check: 9925 from: 1 to: 6664
pFN21AE4181 6664 bp
With 182 enzymes: SgfI *
March 28, 2012 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4474 278
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4007 1937
Size: 2070 4594
Acc65I G'GTAC_C
Cuts at: 3985 3985
Size: 6664
AclI AA'CG_TT
Cuts at: 1563 4791 5164 1563
Size: 3228 373 3063
Fragments arranged by size:
3228 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2747 3051 4727 6111 6379 1632
Size: 1115 304 1676 1384 268 1917
Fragments arranged by size:
1917 1676 1384 1115 304 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6467
AflIII A'CryG_T
Cuts at: 1203 2533 5563 6540 1203
Size: 1330 3030 977 1327
Fragments arranged by size:
3030 1330 1327 977
AgeI A'CCGG_T
Cuts at: 5495 5495
Size: 6664
AhdI GACnn_n'nnGTC
Cuts at: 5393 5393
Size: 6664
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6664
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6664
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2759 2791 2759
Size: 32 6632
AlwNI CAG_nnn'CTG
Cuts at: 2129 2132 2153 2523 3403 4407 5979 2129
Size: 3 21 370 880 1004 1572 2814
Fragments arranged by size:
2814 1572 1004 880 370 21 3
ApaI G_GGCC'C
Cuts at: 2086 2854 2086
Size: 768 5896
ApaLI G'TGCA_C
Cuts at: 4721 5877 4721
Size: 1156 5508
ApoI r'AATT_y
Cuts at: 1493 2867 3791 3973 4199 4351 6460 1493
Size: 1374 924 182 226 152 2109 1697
Fragments arranged by size:
2109 1697 1374 924 226 182 152
AseI AT'TA_AT
Cuts at: 160 1032 5218 160
Size: 872 4186 1606
Fragments arranged by size:
4186 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
AvaI C'yCGr_G
Cuts at: 1870 1943 2079 2085 2858 3989 1870
Size: 73 136 6 773 1131 4545
Fragments arranged by size:
4545 1131 773 136 73 6
BamHI G'GATC_C
Cuts at: 3994 4361 3994
Size: 367 6297
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3985 5440 618
Size: 325 217 627 2198 1455 1842
Fragments arranged by size:
2198 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2036 2086 2186 2854 2912 3803 3983 1391
Size: 645 50 100 668 58 891 180 4072
Fragments arranged by size:
4072 891 668 645 180 100 58 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2850 928
Size: 971 951 4742
Fragments arranged by size:
4742 971 951
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3604 3638 4855 4889 1903
Size: 34 1667 34 1217 34 3678
Fragments arranged by size:
3678 1667 1217 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4556 5766 4556
Size: 1210 5454
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6664
BfrBI ATG'CAT
Cuts at: 3545 3545
Size: 6664
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5275 136
Size: 107 122 71 631 605 3603 1525
Fragments arranged by size:
3603 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2467 6659 2467
Size: 4192 2472
BlpI GC'TnA_GC
Cuts at: 4081 4081
Size: 6664
Bme1580I G_kGCm'C
Cuts at: 1790 2086 2854 3096 3266 4725 5881 1790
Size: 296 768 242 170 1459 1156 2573
Fragments arranged by size:
2573 1459 1156 768 296 242 170
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6664
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2328 2584 3269
Size: 416 53 83 399 24 911 256 685
Cuts at: 3269 3446 4395 5353 442
Size: 177 949 958 1753
Fragments arranged by size:
1753 958 949 911 685 416 399 256
177 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6664
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2121 2121
Size: 6664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2095 2896 3101 3361 5324 1239
Size: 856 801 205 260 1963 2579
Fragments arranged by size:
2579 1963 856 801 260 205
Bpu10I CC'TnA_GC
Cuts at: 2035 2035
Size: 6664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3770 4789 5654 5952 3770
Size: 1019 865 298 4482
Fragments arranged by size:
4482 1019 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2561 2607 5327 882
Size: 617 1062 46 2720 2219
Fragments arranged by size:
2720 2219 1062 617 46
BsaAI yAC'GTr
Cuts at: 493 1204 4455 493
Size: 711 3251 2702
Fragments arranged by size:
3251 2702 711
BsaBI GATnn'nnATC
Cuts at: 6658 6658
Size: 6664
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4471 4853 5488 275
Size: 53 83 186 936 2938 382 635 1451
Fragments arranged by size:
2938 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3360 5097 5495 5769 5916 6327 3360
Size: 1737 398 274 147 411 3697
Fragments arranged by size:
3697 1737 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2405 2435 2759 2786 2789
Size: 30 165 30 1002 30 324 27 3
Cuts at: 2789 2816 3218 3248 3872 3902 1178
Size: 27 402 30 624 30 3940
Fragments arranged by size:
3940 1002 624 402 324 165 30 30
30 30 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2269 2272 2360 2387 2390 2402 2405 2408
Size: 1083 3 88 27 3 12 3 3
Cuts at: 2408 2417 2426 2780 2807 2879 3896 1186
Size: 9 9 354 27 72 1017 3954
Fragments arranged by size:
3954 1083 1017 354 88 72 27 27
12 9 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2681 3185 3276 5867 6363 2681
Size: 504 91 2591 496 2982
Fragments arranged by size:
2982 2591 504 496 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6091
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4114 4875 5903 1540
Size: 275 186 2113 761 1028 2301
Fragments arranged by size:
2301 2113 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2036 2912 3803 4725 4810 5881 2036
Size: 876 891 922 85 1071 2819
Fragments arranged by size:
2819 1071 922 891 876 85
BsmI GAATG_Cn'
Cuts at: 3754 4175 4268 3754
Size: 421 93 6150
Fragments arranged by size:
6150 421 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2086 2186 2854 2912 3096 3266
Size: 399 246 50 100 668 58 184 170
Cuts at: 3266 3803 3983 4725 4810 5881 1391
Size: 537 180 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 668 537 399 246 184
180 170 100 85 58 50
BspEI T'CCGG_A
Cuts at: 3360 6327 3360
Size: 2967 3697
BspHI T'CATG_A
Cuts at: 3213 4553 6283 3213
Size: 1340 1730 3594
Fragments arranged by size:
3594 1730 1340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4019 844
Size: 3175 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2092 4551 1394
Size: 429 269 2459 3507
Fragments arranged by size:
3507 2459 429 269
BsrDI GCAATG_nn'
Cuts at: 65 1248 2022 2314 2659 3301 3639 3657 5159
Size: 1183 774 292 345 642 338 18 1502
Cuts at: 5159 5333 65
Size: 174 1396
Fragments arranged by size:
1502 1396 1183 774 642 345 338 292
174 18
BsrFI r'CCGG_y
Cuts at: 1700 1810 2295 5308 5495 1700
Size: 110 485 3013 187 2869
Fragments arranged by size:
3013 2869 485 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6664
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6664
BssSI C'ACGA_G
Cuts at: 4724 5736 4724
Size: 1012 5652
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2129 3032 794
Size: 1335 903 4426
Fragments arranged by size:
4426 1335 903
BstBI TT'CG_AA
Cuts at: 5468 5468
Size: 6664
BstEII G'GTnAC_C
Cuts at: 1184 3562 1184
Size: 2378 4286
BstYI r'GATC_y
Cuts at: 1972 2352 2467 3013 3363 3399 3994 4361 4746
Size: 380 115 546 350 36 595 367 385
Cuts at: 4746 4763 6215 6301 6313 6659 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 595 546 385 380 367 350
346 115 86 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2357 2478 2357
Size: 121 6543
BtgI C'CryG_G
Cuts at: 513 1065 2004 2443 513
Size: 552 939 439 4734
Fragments arranged by size:
4734 939 552 439
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5925
Fragments arranged by size:
5925 683 56
BtsI GCAGTG_nn'
Cuts at: 4180 4974 4994 4180
Size: 794 20 5850
Fragments arranged by size:
5850 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3930 3965 529
Size: 35 3366 35 3228
Fragments arranged by size:
3366 3228 35 35
DraI TTT'AAA
Cuts at: 2872 3968 2872
Size: 1096 5568
DraIII CAC_nnn'GTG
Cuts at: 3032 3032
Size: 6664
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5671 809
Size: 507 4355 1802
Fragments arranged by size:
4355 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 4111 5000
Size: 54 1425 50 275 18 312 1969 889
Cuts at: 5000 6629 8
Size: 1629 43
Fragments arranged by size:
1969 1629 1425 889 312 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4111 1537
Size: 275 2299 4090
Fragments arranged by size:
4090 2299 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2394 2403 2723 2832 3203 3464 4594 1353
Size: 1041 9 320 109 371 261 1130 3423
Fragments arranged by size:
3423 1130 1041 371 320 261 109 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3521 5251 5625 5771 387
Size: 1064 138 1932 1730 374 146 1280
Fragments arranged by size:
1932 1730 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2034 2910 3801 2034
Size: 876 891 4897
Fragments arranged by size:
4897 891 876
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2747 2896 3051 3101 3361 4727
Size: 393 463 652 149 155 50 260 1366
Cuts at: 4727 5324 6111 6379 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1366 787 652 597 463 393 268
260 155 149 50
EcoO109I rG'GnC_Cy
Cuts at: 2211 2797 2850 2851 3627 2211
Size: 586 53 1 776 5248
Fragments arranged by size:
5248 776 586 53 1
EcoRI G'AATT_C
Cuts at: 3973 4351 3973
Size: 378 6286
EcoRV GAT'ATC
Cuts at: 2755 2755
Size: 6664
FspI TGC'GCA
Cuts at: 2651 5170 2651
Size: 2519 4145
HaeII r_GCGC'y
Cuts at: 1678 5811 1678
Size: 4133 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2899 2931 3218 3250 5305
Size: 32 135 32 1327 32 287 32 2055
Cuts at: 5305 5337 5379 5411 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2055 1327 287 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4008 4254 669
Size: 988 281 2070 246 3079
Fragments arranged by size:
3079 2070 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6664
HpaI GTT'AAC
Cuts at: 4254 4254
Size: 6664
KpnI G_GTAC'C
Cuts at: 3989 3989
Size: 6664
MfeI C'AATT_G
Cuts at: 4263 4263
Size: 6664
MluI A'CGCG_T
Cuts at: 6540 6540
Size: 6664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2231 5778 5962 1305
Size: 926 3547 184 2007
Fragments arranged by size:
3547 2007 926 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4842
Fragments arranged by size:
4842 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2448 3550 4622 4981 5140 518
Size: 648 1282 1102 1072 359 159 2042
Fragments arranged by size:
2042 1282 1102 1072 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2120 2129 2141 2150 2269 4080 4757 5905
Size: 514 9 12 9 119 1811 677 1148
Cuts at: 5905 6150 1606
Size: 245 2120
Fragments arranged by size:
2120 1811 1148 677 514 245 119 12
9 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 2443 513
Size: 552 939 439 4734
Fragments arranged by size:
4734 939 552 439
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6664
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6664
NotI GC'GGCC_GC
Cuts at: 4111 4111
Size: 6664
NsiI A_TGCA'T
Cuts at: 3547 3547
Size: 6664
NspI r_CATG'y
Cuts at: 1132 1229 2056 3430 4022 5567 1132
Size: 97 827 1374 592 1545 2229
Fragments arranged by size:
2229 1545 1374 827 592 97
PasI CC'CwG_GG
Cuts at: 1348 3817 1348
Size: 2469 4195
PciI A'CATG_T
Cuts at: 5563 5563
Size: 6664
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6664
PmeI GTTT'AAAC
Cuts at: 3968 3968
Size: 6664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2759 2791 4733 4765 6246 6278 2759
Size: 32 1942 32 1481 32 3145
Fragments arranged by size:
3145 1942 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3627 3627
Size: 6664
PshAI GACnn'nnGTC
Cuts at: 1613 2269 1613
Size: 656 6008
PsiI TTA'TAA
Cuts at: 4234 4234
Size: 6664
PspOMI G'GGCC_C
Cuts at: 2082 2850 2082
Size: 768 5896
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2155 2187 2155
Size: 32 6632
PstI C_TGCA'G
Cuts at: 830 2125 3399 4016 830
Size: 1295 1274 617 3478
Fragments arranged by size:
3478 1295 1274 617
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6664
PvuII CAG'CTG
Cuts at: 2120 2129 2150 2120
Size: 9 21 6634
Fragments arranged by size:
6634 21 9
SacI G_AGCT'C
Cuts at: 2036 2912 3803 2036
Size: 876 891 4897
Fragments arranged by size:
4897 891 876
SalI G'TCGA_C
Cuts at: 1936 4006 1936
Size: 2070 4594
SanDI GG'GwC_CC
Cuts at: 3627 3627
Size: 6664
SbfI CC_TGCA'GG
Cuts at: 4016 4016
Size: 6664
ScaI AGT'ACT
Cuts at: 2636 4912 6624 2636
Size: 2276 1712 2676
Fragments arranged by size:
2676 2276 1712
SfcI C'TryA_G
Cuts at: 826 1045 2121 2428 2500 2515 3395 3606 4012
Size: 219 1076 307 72 15 880 211 406
Cuts at: 4012 5147 5828 6019 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1076 880 681 406 307 219
211 191 72 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6664
SmaI CCC'GGG
Cuts at: 1872 2081 2087 3991 1872
Size: 209 6 1904 4545
Fragments arranged by size:
4545 1904 209 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3785 4768 5669 5931 820
Size: 197 926 1842 983 901 262 1553
Fragments arranged by size:
1842 1553 983 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4455 493
Size: 3962 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6664
SphI G_CATG'C
Cuts at: 1132 3430 4022 1132
Size: 2298 592 3774
Fragments arranged by size:
3774 2298 592
SrfI GCCC'GGGC
Cuts at: 2081 2081
Size: 6664
SspI AAT'ATT
Cuts at: 5 52 3102 4588 5
Size: 47 3050 1486 2081
Fragments arranged by size:
3050 2081 1486 47
StuI AGG'CCT
Cuts at: 1517 2556 3393 1517
Size: 1039 837 4788
Fragments arranged by size:
4788 1039 837
StyI C'CwwG_G
Cuts at: 513 1065 2004 2443 4103 513
Size: 552 939 439 1660 3074
Fragments arranged by size:
3074 1660 939 552 439
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4691 4708 4861 5046 5491 325
Size: 1255 3111 17 153 185 445 1498
Fragments arranged by size:
3111 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2634 4910 6622
Size: 274 80 33 51 1443 657 2276 1712
Cuts at: 6622 96
Size: 138
Fragments arranged by size:
2276 1712 1443 657 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3854 3872 3886 3904 6624 6656 3854
Size: 18 14 18 2720 32 3862
Fragments arranged by size:
3862 2720 32 18 18 14
Tth111I GACn'n_nGTC
Cuts at: 1316 3230 1316
Size: 1914 4750
XbaI T'CTAG_A
Cuts at: 3838 4000 3838
Size: 162 6502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3546 3574 3546
Size: 28 6636
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6664
XmaI C'CCGG_G
Cuts at: 1870 2079 2085 3989 1870
Size: 209 6 1904 4545
Fragments arranged by size:
4545 1904 209 6
XmnI GAAnn'nnTTC
Cuts at: 4793 4793
Size: 6664
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4472 276
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BsiWI
BsmBI BstXI BstZ17I EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI
RsrII SacII SapI SexAI SfiI SfoI SwaI