Restriction map for
pFN21AE4174
(Circular) (Six-base) MAPSORT of: pFN21AE4174.seq Check: 8409 from: 1 to: 6607
pFN21AE4174 6607 bp
With 182 enzymes: SgfI *
March 28, 2012 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3649 3649
Size: 6607
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4417 278
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2417 3950 1937
Size: 480 1533 4594
Fragments arranged by size:
4594 1533 480
Acc65I G'GTAC_C
Cuts at: 2237 2786 3928 2237
Size: 549 1142 4916
Fragments arranged by size:
4916 1142 549
AclI AA'CG_TT
Cuts at: 1563 4734 5107 1563
Size: 3171 373 3063
Fragments arranged by size:
3171 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2438 3223 3748 4670 6054 6322 1632
Size: 806 785 525 922 1384 268 1917
Fragments arranged by size:
1917 1384 922 806 785 525 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6410
AflIII A'CryG_T
Cuts at: 1203 5506 6483 1203
Size: 4303 977 1327
Fragments arranged by size:
4303 1327 977
AgeI A'CCGG_T
Cuts at: 5438 5438
Size: 6607
AhdI GACnn_n'nnGTC
Cuts at: 5336 5336
Size: 6607
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6607
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6607
AlwNI CAG_nnn'CTG
Cuts at: 3490 4350 5922 3490
Size: 860 1572 4175
Fragments arranged by size:
4175 1572 860
ApaLI G'TGCA_C
Cuts at: 4664 5820 4664
Size: 1156 5451
ApoI r'AATT_y
Cuts at: 1493 2582 2606 2729 3916 4142 4294 6403 1493
Size: 1089 24 123 1187 226 152 2109 1697
Fragments arranged by size:
2109 1697 1187 1089 226 152 123 24
AseI AT'TA_AT
Cuts at: 160 1032 2652 5161 160
Size: 872 1620 2509 1606
Fragments arranged by size:
2509 1620 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
AvaI C'yCGr_G
Cuts at: 1870 1943 3510 3770 3806 3861 3870 3932 1870
Size: 73 1567 260 36 55 9 62 4545
Fragments arranged by size:
4545 1567 260 73 62 55 36 9
BamHI G'GATC_C
Cuts at: 3937 4304 3937
Size: 367 6240
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2237 2786 3649 3928
Size: 325 217 627 221 229 549 863 279
Cuts at: 3928 5383 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 863 627 549 325 279 229
221 217
BanII G_rGCy'C
Cuts at: 1391 2112 3264 3777 3926 1391
Size: 721 1152 513 149 4072
Fragments arranged by size:
4072 1152 721 513 149
BbeI G_GCGC'C
Cuts at: 3653 3653
Size: 6607
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3129 3356 928
Size: 971 1230 227 4179
Fragments arranged by size:
4179 1230 971 227
BbvCI CC'TCA_GC
Cuts at: 3500 3500
Size: 6607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2928 2962 4798 4832 1903
Size: 34 991 34 1836 34 3678
Fragments arranged by size:
3678 1836 991 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3195 4499 5709 3195
Size: 1304 1210 4093
Fragments arranged by size:
4093 1304 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6607
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5218 136
Size: 107 122 71 631 605 3546 1525
Fragments arranged by size:
3546 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2761 6602 2761
Size: 3841 2766
BlpI GC'TnA_GC
Cuts at: 3536 4024 3536
Size: 488 6119
Bme1580I G_kGCm'C
Cuts at: 1790 3327 4668 5824 1790
Size: 1537 1341 1156 2573
Fragments arranged by size:
2573 1537 1341 1156
BmgBI CAC'GTC
Cuts at: 1318 3598 1318
Size: 2280 4327
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4338 5296 442
Size: 416 53 83 399 24 2921 958 1753
Fragments arranged by size:
2921 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3387 1056
Size: 2331 4276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3332 3380 5267 1239
Size: 2093 48 1887 2579
Fragments arranged by size:
2579 2093 1887 48
Bpu10I CC'TnA_GC
Cuts at: 2132 2999 3245 3500 2132
Size: 867 246 255 5239
Fragments arranged by size:
5239 867 255 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4732 5597 5895 4732
Size: 865 298 5444
Fragments arranged by size:
5444 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3015 5270 882
Size: 617 1516 2255 2219
Fragments arranged by size:
2255 2219 1516 617
BsaAI yAC'GTr
Cuts at: 493 1204 3322 3553 4398 493
Size: 711 2118 231 845 2702
Fragments arranged by size:
2702 2118 845 711 231
BsaBI GATnn'nnATC
Cuts at: 6601 6601
Size: 6607
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2974 3353 3650 3681
Size: 53 83 186 936 1441 379 297 31
Cuts at: 3681 4414 4796 5431 275
Size: 733 382 635 1451
Fragments arranged by size:
1451 1441 936 733 635 382 379 297
186 83 53 31
BsaWI w'CCGG_w
Cuts at: 3416 5040 5438 5712 5859 6270 3416
Size: 1624 398 274 147 411 3753
Fragments arranged by size:
3753 1624 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3311 3341 1178
Size: 30 165 30 1908 30 4444
Fragments arranged by size:
4444 1908 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6607
BseYI C'CCAG_C
Cuts at: 3571 5810 6306 3571
Size: 2239 496 3872
Fragments arranged by size:
3872 2239 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2500 2781 3817 1163
Size: 573 764 281 1036 3953
Fragments arranged by size:
3953 1036 764 573 281
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 3393 3403 3630 4057 4818
Size: 275 186 1282 110 10 227 427 761
Cuts at: 4818 5846 1540
Size: 1028 2301
Fragments arranged by size:
2301 1282 1028 761 427 275 227 186
110 10
BsiHKAI G_wGCw'C
Cuts at: 3603 3674 3777 4668 4753 5824 3603
Size: 71 103 891 85 1071 4386
Fragments arranged by size:
4386 1071 891 103 85 71
BsmI GAATG_Cn'
Cuts at: 2101 2702 4118 4211 2101
Size: 601 1416 93 4497
Fragments arranged by size:
4497 1416 601 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3688 3688
Size: 6607
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 3264 3327 3603 3674 3777 3926
Size: 399 322 1152 63 276 71 103 149
Cuts at: 3926 4668 4753 5824 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1152 1071 742 399 322 276 149
103 85 71 63
BspEI T'CCGG_A
Cuts at: 6270 6270
Size: 6607
BspHI T'CATG_A
Cuts at: 4496 6226 4496
Size: 1730 4877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3649 3962 844
Size: 2805 313 3489
Fragments arranged by size:
3489 2805 313
BsrBI CCG'CTC
Cuts at: 1394 1823 4494 1394
Size: 429 2671 3507
Fragments arranged by size:
3507 2671 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2131 2376 2721 2912 5102 5276 65
Size: 1183 883 245 345 191 2190 174 1396
Fragments arranged by size:
2190 1396 1183 883 345 245 191 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3113 3629 5251 5438 1700
Size: 110 1303 516 1622 187 2869
Fragments arranged by size:
2869 1622 1303 516 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6607
BssHII G'CGCG_C
Cuts at: 1925 3368 1925
Size: 1443 5164
BssSI C'ACGA_G
Cuts at: 3263 3478 4667 5679 3263
Size: 215 1189 1012 4191
Fragments arranged by size:
4191 1189 1012 215
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3171 3490 3759 794
Size: 2377 319 269 3642
Fragments arranged by size:
3642 2377 319 269
BstBI TT'CG_AA
Cuts at: 5411 5411
Size: 6607
BstEII G'GTnAC_C
Cuts at: 1184 3412 1184
Size: 2228 4379
BstXI CCAn_nnnn'nTGG
Cuts at: 2182 2182
Size: 6607
BstYI r'GATC_y
Cuts at: 1972 2338 2457 2761 3937 4304 4689 4706 6158
Size: 366 119 304 1176 367 385 17 1452
Cuts at: 6158 6244 6256 6602 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1176 385 367 366 346 304
119 86 17 12
BstZ17I GTA'TAC
Cuts at: 2418 2418
Size: 6607
BtgI C'CryG_G
Cuts at: 513 1065 2004 3273 3606 513
Size: 552 939 1269 333 3514
Fragments arranged by size:
3514 1269 939 552 333
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3412 487
Size: 683 56 2186 3682
Fragments arranged by size:
3682 2186 683 56
BtsI GCAGTG_nn'
Cuts at: 2293 2488 3520 3672 4123 4917 4937 2293
Size: 195 1032 152 451 794 20 3963
Fragments arranged by size:
3963 1032 794 451 195 152 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6607
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2359 2394 529
Size: 35 1795 35 4742
Fragments arranged by size:
4742 1795 35 35
DraI TTT'AAA
Cuts at: 3911 3911
Size: 6607
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5614 809
Size: 507 4298 1802
Fragments arranged by size:
4298 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2367 3210 3280
Size: 54 1425 50 275 18 537 843 70
Cuts at: 3280 3400 3468 3631 3697 4054 4943 6572 8
Size: 120 68 163 66 357 889 1629 43
Fragments arranged by size:
1629 1425 889 843 537 357 275 163
120 70 68 66 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 3400 4054 1537
Size: 275 1468 120 654 4090
Fragments arranged by size:
4090 1468 654 275 120
EarI CTCTTCn'nnn_
Cuts at: 1353 4537 1353
Size: 3184 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5194 5568 5714 387
Size: 1064 138 3605 374 146 1280
Fragments arranged by size:
3605 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3775 3775
Size: 6607
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2438 3223 3332 3380 3748 4670 5267
Size: 393 806 785 109 48 368 922 597
Cuts at: 5267 6054 6322 1239
Size: 787 268 1524
Fragments arranged by size:
1524 922 806 787 785 597 393 368
268 109 48
EcoRI G'AATT_C
Cuts at: 3916 4294 3916
Size: 378 6229
EcoRV GAT'ATC
Cuts at: 2047 2350 2047
Size: 303 6304
FspI TGC'GCA
Cuts at: 5113 5113
Size: 6607
HaeII r_GCGC'y
Cuts at: 1678 3653 3692 5754 1678
Size: 1975 39 2062 2531
Fragments arranged by size:
2531 2062 1975 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2323 2355 2449 2481 2943
Size: 32 135 32 751 32 94 32 462
Cuts at: 2943 2975 3411 3443 5248 5280 5322 5354 1373
Size: 32 436 32 1805 32 42 32 2626
Fragments arranged by size:
2626 1805 751 462 436 135 94 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3951 4197 669
Size: 988 281 2013 246 3079
Fragments arranged by size:
3079 2013 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3044 748
Size: 2296 4311
HpaI GTT'AAC
Cuts at: 4197 4197
Size: 6607
KasI G'GCGC_C
Cuts at: 3649 3649
Size: 6607
KpnI G_GTAC'C
Cuts at: 2241 2790 3932 2241
Size: 549 1142 4916
Fragments arranged by size:
4916 1142 549
MfeI C'AATT_G
Cuts at: 4206 4206
Size: 6607
MluI A'CGCG_T
Cuts at: 6483 6483
Size: 6607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5721 5905 1305
Size: 4416 184 2007
Fragments arranged by size:
4416 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2369 3212 10
Size: 54 1425 343 537 843 3405
Fragments arranged by size:
3405 1425 843 537 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4565 4924 5083 518
Size: 648 3399 359 159 2042
Fragments arranged by size:
3399 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3275 3571 3655 4023 4700 5848 6093 1606
Size: 1669 296 84 368 677 1148 245 2120
Fragments arranged by size:
2120 1669 1148 677 368 296 245 84
NarI GG'CG_CC
Cuts at: 3650 3650
Size: 6607
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5116
Fragments arranged by size:
5116 939 552
NdeI CA'TA_TG
Cuts at: 387 2383 387
Size: 1996 4611
NheI G'CTAG_C
Cuts at: 1052 3383 1052
Size: 2331 4276
NotI GC'GGCC_GC
Cuts at: 3280 4054 3280
Size: 774 5833
NspI r_CATG'y
Cuts at: 1132 1229 2524 3158 3965 5510 1132
Size: 97 1295 634 807 1545 2229
Fragments arranged by size:
2229 1545 1295 807 634 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6607
PciI A'CATG_T
Cuts at: 5506 5506
Size: 6607
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3339 1108
Size: 2231 4376
PmeI GTTT'AAAC
Cuts at: 3911 3911
Size: 6607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4676 4708 6189 6221 4676
Size: 32 1481 32 5062
Fragments arranged by size:
5062 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6607
PsiI TTA'TAA
Cuts at: 2471 4177 2471
Size: 1706 4901
PstI C_TGCA'G
Cuts at: 830 2761 3176 3660 3959 830
Size: 1931 415 484 299 3478
Fragments arranged by size:
3478 1931 484 415 299
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6607
PvuII CAG'CTG
Cuts at: 3571 3571
Size: 6607
SacI G_AGCT'C
Cuts at: 3777 3777
Size: 6607
SacII CC_GC'GG
Cuts at: 3276 3276
Size: 6607
SalI G'TCGA_C
Cuts at: 1936 3949 1936
Size: 2013 4594
SbfI CC_TGCA'GG
Cuts at: 3959 3959
Size: 6607
ScaI AGT'ACT
Cuts at: 4855 6567 4855
Size: 1712 4895
SexAI A'CCwGG_T
Cuts at: 2805 3708 2805
Size: 903 5704
SfcI C'TryA_G
Cuts at: 826 1045 2757 3172 3616 3656 3955 5090 5771
Size: 219 1712 415 444 40 299 1135 681
Cuts at: 5771 5962 826
Size: 191 1471
Fragments arranged by size:
1712 1471 1135 681 444 415 299 219
191 40
SfoI GGC'GCC
Cuts at: 3651 3651
Size: 6607
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6607
SmaI CCC'GGG
Cuts at: 1872 3512 3872 3934 1872
Size: 1640 360 62 4545
Fragments arranged by size:
4545 1640 360 62
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4711 5612 5874 820
Size: 197 926 2768 901 262 1553
Fragments arranged by size:
2768 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4398 493
Size: 3905 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6607
SphI G_CATG'C
Cuts at: 1132 3158 3965 1132
Size: 2026 807 3774
Fragments arranged by size:
3774 2026 807
SspI AAT'ATT
Cuts at: 5 52 2536 2776 4531 5
Size: 47 2484 240 1755 2081
Fragments arranged by size:
2484 2081 1755 240 47
StuI AGG'CCT
Cuts at: 1517 2364 2982 1517
Size: 847 618 5142
Fragments arranged by size:
5142 847 618
StyI C'CwwG_G
Cuts at: 513 1065 2004 4046 513
Size: 552 939 2042 3074
Fragments arranged by size:
3074 2042 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 4634 4651 4804 4989 5434 325
Size: 1255 446 2608 17 153 185 445 1498
Fragments arranged by size:
2608 1498 1255 446 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3741 4853 6565
Size: 274 80 33 51 1443 1764 1112 1712
Cuts at: 6565 96
Size: 138
Fragments arranged by size:
1764 1712 1443 1112 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6567 6599 6567
Size: 32 6575
Tth111I GACn'n_nGTC
Cuts at: 1316 2228 2807 1316
Size: 912 579 5116
Fragments arranged by size:
5116 912 579
XbaI T'CTAG_A
Cuts at: 3943 3943
Size: 6607
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6607
XmaI C'CCGG_G
Cuts at: 1870 3510 3870 3932 1870
Size: 1640 360 62 4545
Fragments arranged by size:
4545 1640 360 62
XmnI GAAnn'nnTTC
Cuts at: 4736 4736
Size: 6607
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4415 276
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SanDI SapI SfiI SrfI
SwaI XcmI