Restriction map for
pFN21AE4168
(Circular) (Six-base) MAPSORT of: pFN21AE4168.seq Check: 5907 from: 1 to: 6565
pFN21AE4168 6565 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4375 278
Size: 53 83 186 936 2839 2468
Fragments arranged by size:
2839 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3459 3908 1937
Size: 1522 449 4594
Fragments arranged by size:
4594 1522 449
Acc65I G'GTAC_C
Cuts at: 3886 3886
Size: 6565
AclI AA'CG_TT
Cuts at: 1563 4692 5065 1563
Size: 3129 373 3063
Fragments arranged by size:
3129 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2549 2558 2606 4628 6012 6280 1632
Size: 917 9 48 2022 1384 268 1917
Fragments arranged by size:
2022 1917 1384 917 268 48 9
AfeI AGC'GCT
Cuts at: 2097 2097
Size: 6565
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6368
AflIII A'CryG_T
Cuts at: 1203 3089 3584 5464 6441 1203
Size: 1886 495 1880 977 1327
Fragments arranged by size:
1886 1880 1327 977 495
AgeI A'CCGG_T
Cuts at: 5396 5396
Size: 6565
AhdI GACnn_n'nnGTC
Cuts at: 3108 5294 3108
Size: 2186 4379
AleI CACnn'nnGTG
Cuts at: 1166 3021 1166
Size: 1855 4710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6565
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2145 2177 2145
Size: 32 6533
AlwNI CAG_nnn'CTG
Cuts at: 2293 3241 3709 4308 5880 2293
Size: 948 468 599 1572 2978
Fragments arranged by size:
2978 1572 948 599 468
ApaLI G'TGCA_C
Cuts at: 4622 5778 4622
Size: 1156 5409
ApoI r'AATT_y
Cuts at: 1493 2159 3874 4100 4252 6361 1493
Size: 666 1715 226 152 2109 1697
Fragments arranged by size:
2109 1715 1697 666 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5119 160
Size: 872 4087 1606
Fragments arranged by size:
4087 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
AvaI C'yCGr_G
Cuts at: 1870 1943 3890 1870
Size: 73 1947 4545
Fragments arranged by size:
4545 1947 73
BamHI G'GATC_C
Cuts at: 3534 3895 4262 3534
Size: 361 367 5837
Fragments arranged by size:
5837 367 361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3185 3857 3886 5341 618
Size: 325 217 627 1398 672 29 1455 1842
Fragments arranged by size:
1842 1455 1398 672 627 325 217 29
BanII G_rGCy'C
Cuts at: 1391 2685 3884 1391
Size: 1294 1199 4072
Fragments arranged by size:
4072 1294 1199
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3335 928
Size: 971 1436 4158
Fragments arranged by size:
4158 1436 971
BbvCI CC'TCA_GC
Cuts at: 2148 2148
Size: 6565
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4756 4790 1903
Size: 34 50 34 2735 34 3678
Fragments arranged by size:
3678 2735 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2713 3710 4457 5667 2713
Size: 997 747 1210 3611
Fragments arranged by size:
3611 1210 997 747
BclI T'GATC_A
Cuts at: 1548 2944 1548
Size: 1396 5169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2833 5176 136
Size: 107 122 71 631 605 1161 2343 1525
Fragments arranged by size:
2343 1525 1161 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2622 6560 2622
Size: 3938 2627
BlpI GC'TnA_GC
Cuts at: 3982 3982
Size: 6565
Bme1580I G_kGCm'C
Cuts at: 1790 4626 5782 1790
Size: 2836 1156 2573
Fragments arranged by size:
2836 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3090 1318
Size: 1772 4793
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4296 5254 442
Size: 416 53 83 399 24 2879 958 1753
Fragments arranged by size:
2879 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2354 2636 1056
Size: 1298 282 4985
Fragments arranged by size:
4985 1298 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2345 3001 3645 5225 1239
Size: 1076 30 656 644 1580 2579
Fragments arranged by size:
2579 1580 1076 656 644 30
Bpu10I CC'TnA_GC
Cuts at: 2148 2148
Size: 6565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4690 5555 5853 4690
Size: 865 298 5402
Fragments arranged by size:
5402 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5228 882
Size: 617 3729 2219
Fragments arranged by size:
3729 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4356 493
Size: 711 3152 2702
Fragments arranged by size:
3152 2702 711
BsaBI GATnn'nnATC
Cuts at: 6559 6559
Size: 6565
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4372 4754 5389 275
Size: 53 83 186 936 2839 382 635 1451
Fragments arranged by size:
2839 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3531 4998 5396 5670 5817 6228 3531
Size: 1467 398 274 147 411 3868
Fragments arranged by size:
3868 1467 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3051 3081 3618 3648 1178
Size: 30 165 30 1648 30 537 30 4095
Fragments arranged by size:
4095 1648 537 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2125 1186
Size: 939 5626
BseYI C'CCAG_C
Cuts at: 3181 5768 6264 3181
Size: 2587 496 3482
Fragments arranged by size:
3482 2587 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5992
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2596 4015 4776 5804 1540
Size: 275 186 595 1419 761 1028 2301
Fragments arranged by size:
2301 1419 1028 761 595 275 186
BsiHKAI G_wGCw'C
Cuts at: 2685 4626 4711 5782 2685
Size: 1941 85 1071 3468
Fragments arranged by size:
3468 1941 1071 85
BsmI GAATG_Cn'
Cuts at: 2959 4076 4169 2959
Size: 1117 93 5355
Fragments arranged by size:
5355 1117 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2685 3884 4626 4711 5782 1391
Size: 399 895 1199 742 85 1071 2174
Fragments arranged by size:
2174 1199 1071 895 742 399 85
BspEI T'CCGG_A
Cuts at: 6228 6228
Size: 6565
BspHI T'CATG_A
Cuts at: 4454 6184 4454
Size: 1730 4835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3609 3920 844
Size: 2765 311 3489
Fragments arranged by size:
3489 2765 311
BsrBI CCG'CTC
Cuts at: 1394 1823 4452 1394
Size: 429 2629 3507
Fragments arranged by size:
3507 2629 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2217 2970 3514 5060 5234 65
Size: 1183 969 753 544 1546 174 1396
Fragments arranged by size:
1546 1396 1183 969 753 544 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5209 5396 1700
Size: 110 3399 187 2869
Fragments arranged by size:
3399 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2445 3478 96
Size: 2349 1033 3183
Fragments arranged by size:
3183 2349 1033
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6565
BssSI C'ACGA_G
Cuts at: 4625 5637 4625
Size: 1012 5553
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2127 3178 794
Size: 1333 1051 4181
Fragments arranged by size:
4181 1333 1051
BstBI TT'CG_AA
Cuts at: 5369 5369
Size: 6565
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6565
BstXI CCAn_nnnn'nTGG
Cuts at: 3004 3004
Size: 6565
BstYI r'GATC_y
Cuts at: 1972 2440 2622 3305 3534 3895 4262 4647 4664
Size: 468 182 683 229 361 367 385 17
Cuts at: 4664 6116 6202 6214 6560 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 683 468 385 367 361 346
229 182 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3189 3468 513
Size: 552 2124 279 3610
Fragments arranged by size:
3610 2124 552 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5826
Fragments arranged by size:
5826 683 56
BtsI GCAGTG_nn'
Cuts at: 2200 2496 4081 4875 4895 2200
Size: 296 1585 794 20 3870
Fragments arranged by size:
3870 1585 794 296 20
ClaI AT'CG_AT
Cuts at: 1554 2496 1554
Size: 942 5623
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6530
DraI TTT'AAA
Cuts at: 3869 3869
Size: 6565
DraIII CAC_nnn'GTG
Cuts at: 2266 2266
Size: 6565
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5572 809
Size: 507 4256 1802
Fragments arranged by size:
4256 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3082 3610 4012
Size: 54 1425 50 275 18 1252 528 402
Cuts at: 4012 4901 6530 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1252 889 528 402 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4012 1537
Size: 275 2200 4090
Fragments arranged by size:
4090 2200 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2525 2582 2610 3837 4495 1353
Size: 1172 57 28 1227 658 3423
Fragments arranged by size:
3423 1227 1172 658 57 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5152 5526 5672 387
Size: 1064 138 3563 374 146 1280
Fragments arranged by size:
3563 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2683 2683
Size: 6565
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2345 2549 2558 2606 3001 3645
Size: 393 683 30 204 9 48 395 644
Cuts at: 3645 4628 5225 6012 6280 1239
Size: 983 597 787 268 1524
Fragments arranged by size:
1524 983 787 683 644 597 395 393
268 204 48 30 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2573 2573
Size: 6565
EcoO109I rG'GnC_Cy
Cuts at: 2794 2794
Size: 6565
EcoRI G'AATT_C
Cuts at: 3874 4252 3874
Size: 378 6187
EcoRV GAT'ATC
Cuts at: 3354 3354
Size: 6565
FspI TGC'GCA
Cuts at: 5071 5071
Size: 6565
HaeII r_GCGC'y
Cuts at: 1678 2099 5712 1678
Size: 421 3613 2531
Fragments arranged by size:
3613 2531 421
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3094 3126 3618 3650 5206
Size: 32 135 32 1522 32 492 32 1556
Cuts at: 5206 5238 5280 5312 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1556 1522 492 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2788 3909 4155 669
Size: 988 281 850 1121 246 3079
Fragments arranged by size:
3079 1121 988 850 281 246
HindIII A'AGCT_T
Cuts at: 748 3488 748
Size: 2740 3825
HpaI GTT'AAC
Cuts at: 4155 4155
Size: 6565
KpnI G_GTAC'C
Cuts at: 3890 3890
Size: 6565
MfeI C'AATT_G
Cuts at: 4164 4164
Size: 6565
MluI A'CGCG_T
Cuts at: 6441 6441
Size: 6565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2358 2460 2628 5679 5863 1305
Size: 1053 102 168 3051 184 2007
Fragments arranged by size:
3051 2007 1053 184 168 102
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3612 10
Size: 54 1425 343 1780 2963
Fragments arranged by size:
2963 1780 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3002 3021 3078 4523 4882 5041 518
Size: 648 1836 19 57 1445 359 159 2042
Fragments arranged by size:
2042 1836 1445 648 359 159 57 19
MspA1I CmG'CkG
Cuts at: 1606 2357 2374 3981 4658 5806 6051 1606
Size: 751 17 1607 677 1148 245 2120
Fragments arranged by size:
2120 1607 1148 751 677 245 17
NcoI C'CATG_G
Cuts at: 513 1065 3189 3468 513
Size: 552 2124 279 3610
Fragments arranged by size:
3610 2124 552 279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6565
NheI G'CTAG_C
Cuts at: 1052 2350 2632 1052
Size: 1298 282 4985
Fragments arranged by size:
4985 1298 282
NotI GC'GGCC_GC
Cuts at: 4012 4012
Size: 6565
NspI r_CATG'y
Cuts at: 1132 1229 2193 3077 3789 3816 3923 5468 1132
Size: 97 964 884 712 27 107 1545 2229
Fragments arranged by size:
2229 1545 964 884 712 107 97 27
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6565
PciI A'CATG_T
Cuts at: 5464 5464
Size: 6565
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2674 3025 3469 3709 1108
Size: 1566 351 444 240 3964
Fragments arranged by size:
3964 1566 444 351 240
PfoI T'CCnGG_A
Cuts at: 2340 2412 2340
Size: 72 6493
PmeI GTTT'AAAC
Cuts at: 3869 3869
Size: 6565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2007 2039 4634 4666 6147 6179 2007
Size: 32 2595 32 1481 32 2393
Fragments arranged by size:
2595 2393 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6565
PsiI TTA'TAA
Cuts at: 4135 4135
Size: 6565
PstI C_TGCA'G
Cuts at: 830 3450 3917 830
Size: 2620 467 3478
Fragments arranged by size:
3478 2620 467
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6565
PvuII CAG'CTG
Cuts at: 2374 2374
Size: 6565
SacI G_AGCT'C
Cuts at: 2685 2685
Size: 6565
SalI G'TCGA_C
Cuts at: 1936 3907 1936
Size: 1971 4594
SapI GCTCTTCn'nnn_
Cuts at: 2525 2582 2610 2525
Size: 57 28 6480
Fragments arranged by size:
6480 57 28
SbfI CC_TGCA'GG
Cuts at: 3917 3917
Size: 6565
ScaI AGT'ACT
Cuts at: 4813 6525 4813
Size: 1712 4853
SfcI C'TryA_G
Cuts at: 826 1045 2369 3246 3446 3913 5048 5729 5920
Size: 219 1324 877 200 467 1135 681 191
Cuts at: 5920 826
Size: 1471
Fragments arranged by size:
1471 1324 1135 877 681 467 219 200
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6565
SmaI CCC'GGG
Cuts at: 1872 3892 1872
Size: 2020 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4669 5570 5832 820
Size: 197 926 2726 901 262 1553
Fragments arranged by size:
2726 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4356 493
Size: 3863 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6565
SphI G_CATG'C
Cuts at: 1132 2193 3077 3789 3923 1132
Size: 1061 884 712 134 3774
Fragments arranged by size:
3774 1061 884 712 134
SspI AAT'ATT
Cuts at: 5 52 4489 5
Size: 47 4437 2081
Fragments arranged by size:
4437 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6565
StyI C'CwwG_G
Cuts at: 513 1065 2275 3189 3468 4004 513
Size: 552 1210 914 279 536 3074
Fragments arranged by size:
3074 1210 914 552 536 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3166 3173 4592 4609 4762 4947 5392
Size: 1255 1586 7 1419 17 153 185 445
Cuts at: 5392 325
Size: 1498
Fragments arranged by size:
1586 1498 1419 1255 445 185 153 17
7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2445 2670 3478
Size: 274 80 33 51 1443 468 225 808
Cuts at: 3478 4811 6523 96
Size: 1333 1712 138
Fragments arranged by size:
1712 1443 1333 808 468 274 225 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3343 3375 6525 6557 3343
Size: 32 3150 32 3351
Fragments arranged by size:
3351 3150 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2084 1316
Size: 768 5797
XbaI T'CTAG_A
Cuts at: 3901 3901
Size: 6565
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3620 3620
Size: 6565
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6565
XmaI C'CCGG_G
Cuts at: 1870 3890 1870
Size: 2020 4545
XmnI GAAnn'nnTTC
Cuts at: 2547 4694 2547
Size: 2147 4418
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4373 276
Size: 53 83 186 936 2839 2468
Fragments arranged by size:
2839 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI