Restriction map for
pFN21AE4167
(Circular) (Six-base) MAPSORT of: pFN21AE4167.seq Check: 4403 from: 1 to: 6562
pFN21AE4167 6562 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3399 3399
Size: 6562
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4372 278
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3905 1937
Size: 1968 4594
Acc65I G'GTAC_C
Cuts at: 3883 3883
Size: 6562
AclI AA'CG_TT
Cuts at: 1563 3861 4689 5062 1563
Size: 2298 828 373 3063
Fragments arranged by size:
3063 2298 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3098 4625 6009 6277 1632
Size: 1466 1527 1384 268 1917
Fragments arranged by size:
1917 1527 1466 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6365
AflIII A'CryG_T
Cuts at: 1203 2367 2940 3180 5461 6438 1203
Size: 1164 573 240 2281 977 1327
Fragments arranged by size:
2281 1327 1164 977 573 240
AgeI A'CCGG_T
Cuts at: 5393 5393
Size: 6562
AhdI GACnn_n'nnGTC
Cuts at: 5291 5291
Size: 6562
AleI CACnn'nnGTG
Cuts at: 1166 2055 3335 1166
Size: 889 1280 4393
Fragments arranged by size:
4393 1280 889
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3589 1184
Size: 2405 4157
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 6530
AlwNI CAG_nnn'CTG
Cuts at: 4305 5877 4305
Size: 1572 4990
ApaI G_GGCC'C
Cuts at: 3489 3587 3489
Size: 98 6464
ApaLI G'TGCA_C
Cuts at: 3184 3293 4619 5775 3184
Size: 109 1326 1156 3971
Fragments arranged by size:
3971 1326 1156 109
ApoI r'AATT_y
Cuts at: 1493 3871 4097 4249 6358 1493
Size: 2378 226 152 2109 1697
Fragments arranged by size:
2378 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5116 160
Size: 872 4084 1606
Fragments arranged by size:
4084 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
AvaI C'yCGr_G
Cuts at: 1870 1943 3580 3887 1870
Size: 73 1637 307 4545
Fragments arranged by size:
4545 1637 307 73
BamHI G'GATC_C
Cuts at: 3892 4259 3892
Size: 367 6195
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2201 2798 2999 3623 3883
Size: 325 217 627 414 597 201 624 260
Cuts at: 3883 5338 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 624 597 414 325 260
217 201
BanII G_rGCy'C
Cuts at: 1391 2028 3489 3587 3819 3881 1391
Size: 637 1461 98 232 62 4072
Fragments arranged by size:
4072 1461 637 232 98 62
BbeI G_GCGC'C
Cuts at: 3627 3627
Size: 6562
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5591
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2120 2154 2981 3015 4753 4787 1903
Size: 34 183 34 827 34 1738 34 3678
Fragments arranged by size:
3678 1738 827 183 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4454 5664 4454
Size: 1210 5352
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6562
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2962 3558 3582
Size: 107 122 71 631 605 1290 596 24
Cuts at: 3582 3800 3804 5173 136
Size: 218 4 1369 1525
Fragments arranged by size:
1525 1369 1290 631 605 596 218 122
107 71 24 4
BglII A'GATC_T
Cuts at: 6557 6557
Size: 6562
BlpI GC'TnA_GC
Cuts at: 3979 3979
Size: 6562
Bme1580I G_kGCm'C
Cuts at: 1790 3104 3188 3297 3489 3587 4623 5779 1790
Size: 1314 84 109 192 98 1036 1156 2573
Fragments arranged by size:
2573 1314 1156 1036 192 109 98 84
BmgBI CAC'GTC
Cuts at: 1318 2110 1318
Size: 792 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4293 5251 442
Size: 416 53 83 399 24 2876 958 1753
Fragments arranged by size:
2876 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 2366 2396 3665 3716 5222 1239
Size: 1052 75 30 1269 51 1506 2579
Fragments arranged by size:
2579 1506 1269 1052 75 51 30
Bpu10I CC'TnA_GC
Cuts at: 2696 2817 3629 2696
Size: 121 812 5629
Fragments arranged by size:
5629 812 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3293 4687 5552 5850 3293
Size: 1394 865 298 4005
Fragments arranged by size:
4005 1394 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2731 5225 882
Size: 617 1232 2494 2219
Fragments arranged by size:
2494 2219 1232 617
BsaAI yAC'GTr
Cuts at: 493 1204 3835 4353 493
Size: 711 2631 518 2702
Fragments arranged by size:
2702 2631 711 518
BsaBI GATnn'nnATC
Cuts at: 6556 6556
Size: 6562
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3624 4369 4751 5386
Size: 53 83 186 936 2091 745 382 635
Cuts at: 5386 275
Size: 1451
Fragments arranged by size:
2091 1451 936 745 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4995 5393 5667 5814 6225 4995
Size: 398 274 147 411 5332
Fragments arranged by size:
5332 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2150 2339 2369 2693
Size: 30 165 30 717 30 189 30 324
Cuts at: 2693 2723 3638 3668 3824 3854 1178
Size: 30 915 30 156 30 3886
Fragments arranged by size:
3886 915 717 324 189 165 156 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2423 2471 2483 2486 2489 2498 2501 2891
Size: 1237 48 12 3 3 9 3 390
Cuts at: 2891 2894 2903 3674 1186
Size: 3 9 771 4074
Fragments arranged by size:
4074 1237 771 390 48 12 9 9
3 3 3 3
BseYI C'CCAG_C
Cuts at: 2808 3535 3587 5765 6261 2808
Size: 727 52 2178 496 3109
Fragments arranged by size:
3109 2178 727 496 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2089 2144 3377 1163
Size: 573 353 55 1233 4348
Fragments arranged by size:
4348 1233 573 353 55
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3445 4012 4773 5801 1540
Size: 275 186 1444 567 761 1028 2301
Fragments arranged by size:
2301 1444 1028 761 567 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 2115 3188 3297 4623 4708 5779 2028
Size: 87 1073 109 1326 85 1071 2811
Fragments arranged by size:
2811 1326 1073 1071 109 87 85
BsmI GAATG_Cn'
Cuts at: 2034 4073 4166 2034
Size: 2039 93 4430
Fragments arranged by size:
4430 2039 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2115 3104 3188 3297 3489 3587
Size: 399 238 87 989 84 109 192 98
Cuts at: 3587 3819 3881 4623 4708 5779 1391
Size: 232 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 989 742 399 238 232 192
109 98 87 85 84 62
BspEI T'CCGG_A
Cuts at: 6225 6225
Size: 6562
BspHI T'CATG_A
Cuts at: 4451 6181 4451
Size: 1730 4832
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2961 3399 3917 844
Size: 2117 438 518 3489
Fragments arranged by size:
3489 2117 518 438
BsrBI CCG'CTC
Cuts at: 1394 1823 2151 3256 3649 4449 1394
Size: 429 328 1105 393 800 3507
Fragments arranged by size:
3507 1105 800 429 393 328
BsrDI GCAATG_nn'
Cuts at: 65 1248 2317 5057 5231 65
Size: 1183 1069 2740 174 1396
Fragments arranged by size:
2740 1396 1183 1069 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2198 3794 3803 3821 5206 5393 1700
Size: 110 388 1596 9 18 1385 187 2869
Fragments arranged by size:
2869 1596 1385 388 187 110 18 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6562
BssHII G'CGCG_C
Cuts at: 1925 3685 3687 1925
Size: 1760 2 4800
Fragments arranged by size:
4800 1760 2
BssSI C'ACGA_G
Cuts at: 4622 5634 4622
Size: 1012 5550
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3192 3276 794
Size: 2398 84 4080
Fragments arranged by size:
4080 2398 84
BstBI TT'CG_AA
Cuts at: 5366 5366
Size: 6562
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6562
BstXI CCAn_nnnn'nTGG
Cuts at: 2344 2781 3031 2344
Size: 437 250 5875
Fragments arranged by size:
5875 437 250
BstYI r'GATC_y
Cuts at: 1972 2349 2845 3892 4259 4644 4661 6113 6199
Size: 377 496 1047 367 385 17 1452 86
Cuts at: 6199 6211 6557 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1047 496 385 377 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2250 2250
Size: 6562
BtgI C'CryG_G
Cuts at: 513 1065 2004 2826 3143 513
Size: 552 939 822 317 3932
Fragments arranged by size:
3932 939 822 552 317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5823
Fragments arranged by size:
5823 683 56
BtsI GCAGTG_nn'
Cuts at: 2062 3104 3274 4078 4872 4892 2062
Size: 1042 170 804 794 20 3732
Fragments arranged by size:
3732 1042 804 794 170 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6562
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6527
DraI TTT'AAA
Cuts at: 3866 3866
Size: 6562
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5569 809
Size: 507 4253 1802
Fragments arranged by size:
4253 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2653 2783
Size: 54 1425 50 275 18 177 646 130
Cuts at: 2783 3442 3550 3738 3801 4009 4898 6527 8
Size: 659 108 188 63 208 889 1629 43
Fragments arranged by size:
1629 1425 889 659 646 275 208 188
177 130 108 63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3442 4009 1537
Size: 275 1630 567 4090
Fragments arranged by size:
4090 1630 567 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2460 2475 3047 3060 3485 4492 1353
Size: 1107 15 572 13 425 1007 3423
Fragments arranged by size:
3423 1107 1007 572 425 15 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2999 5149 5523 5669 387
Size: 1064 138 1410 2150 374 146 1280
Fragments arranged by size:
2150 1410 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2026 2026
Size: 6562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2366 2396 3098 3665 3716 4625
Size: 393 659 75 30 702 567 51 909
Cuts at: 4625 5222 6009 6277 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 909 787 702 659 597 567 393
268 75 51 30
EcoO109I rG'GnC_Cy
Cuts at: 2062 2963 2972 3156 3584 3718 2062
Size: 901 9 184 428 134 4906
Fragments arranged by size:
4906 901 428 184 134 9
EcoRI G'AATT_C
Cuts at: 3871 4249 3871
Size: 378 6184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3532 3532
Size: 6562
FseI GG_CCGG'CC
Cuts at: 3807 3807
Size: 6562
FspI TGC'GCA
Cuts at: 3323 5068 3323
Size: 1745 4817
FspAI rTGC'GCAy
Cuts at: 3323 3323
Size: 6562
HaeII r_GCGC'y
Cuts at: 1678 3412 3617 3627 5709 1678
Size: 1734 205 10 2082 2531
Fragments arranged by size:
2531 2082 1734 205 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2569 2601 5203 5235 5277
Size: 32 135 32 997 32 2602 32 42
Cuts at: 5277 5309 1373
Size: 32 2626
Fragments arranged by size:
2626 2602 997 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3906 4152 669
Size: 988 281 1968 246 3079
Fragments arranged by size:
3079 1968 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2186 748
Size: 1438 5124
HpaI GTT'AAC
Cuts at: 4152 4152
Size: 6562
KasI G'GCGC_C
Cuts at: 3623 3623
Size: 6562
KpnI G_GTAC'C
Cuts at: 3887 3887
Size: 6562
MfeI C'AATT_G
Cuts at: 4161 4161
Size: 6562
MluI A'CGCG_T
Cuts at: 6438 6438
Size: 6562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5676 5860 1305
Size: 4371 184 2007
Fragments arranged by size:
4371 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2655 2785 10
Size: 54 1425 343 177 646 130 3787
Fragments arranged by size:
3787 1425 646 343 177 130 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2055 3181 3335 4520 4879 5038 518
Size: 648 889 1126 154 1185 359 159 2042
Fragments arranged by size:
2042 1185 1126 889 648 359 159 154
MspA1I CmG'CkG
Cuts at: 1606 2306 2556 2812 3145 3569 3694 3775 3978
Size: 700 250 256 333 424 125 81 203
Cuts at: 3978 4655 5803 6048 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 700 677 424 333 256 250
245 203 125 81
NaeI GCC'GGC
Cuts at: 2200 3796 3805 3823 2200
Size: 1596 9 18 4939
Fragments arranged by size:
4939 1596 18 9
NarI GG'CG_CC
Cuts at: 3624 3624
Size: 6562
NcoI C'CATG_G
Cuts at: 513 1065 2004 2826 513
Size: 552 939 822 4249
Fragments arranged by size:
4249 939 822 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6562
NgoMIV G'CCGG_C
Cuts at: 2198 3794 3803 3821 2198
Size: 1596 9 18 4939
Fragments arranged by size:
4939 1596 18 9
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6562
NotI GC'GGCC_GC
Cuts at: 3442 4009 3442
Size: 567 5995
NspI r_CATG'y
Cuts at: 1132 1229 2944 3184 3238 3322 3920 5465 1132
Size: 97 1715 240 54 84 598 1545 2229
Fragments arranged by size:
2229 1715 1545 598 240 97 84 54
PasI CC'CwG_GG
Cuts at: 1348 2616 3812 1348
Size: 1268 1196 4098
Fragments arranged by size:
4098 1268 1196
PciI A'CATG_T
Cuts at: 2940 3180 5461 2940
Size: 240 2281 4041
Fragments arranged by size:
4041 2281 240
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6562
PfoI T'CCnGG_A
Cuts at: 2268 2670 2268
Size: 402 6160
PmeI GTTT'AAAC
Cuts at: 3866 3866
Size: 6562
PmlI CAC'GTG
Cuts at: 3835 3835
Size: 6562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 4631 4663 6144 6176 3638
Size: 32 961 32 1481 32 4024
Fragments arranged by size:
4024 1481 961 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2062 2972 3156 2062
Size: 910 184 5468
Fragments arranged by size:
5468 910 184
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6562
PsiI TTA'TAA
Cuts at: 4132 4132
Size: 6562
PspOMI G'GGCC_C
Cuts at: 3485 3583 3485
Size: 98 6464
PstI C_TGCA'G
Cuts at: 830 2171 3914 830
Size: 1341 1743 3478
Fragments arranged by size:
3478 1743 1341
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6562
PvuII CAG'CTG
Cuts at: 2556 2812 3569 3694 2556
Size: 256 757 125 5424
Fragments arranged by size:
5424 757 256 125
SacI G_AGCT'C
Cuts at: 2028 2028
Size: 6562
SacII CC_GC'GG
Cuts at: 3146 3146
Size: 6562
SalI G'TCGA_C
Cuts at: 1936 3904 1936
Size: 1968 4594
SanDI GG'GwC_CC
Cuts at: 2972 2972
Size: 6562
SapI GCTCTTCn'nnn_
Cuts at: 3060 3485 3060
Size: 425 6137
SbfI CC_TGCA'GG
Cuts at: 3914 3914
Size: 6562
ScaI AGT'ACT
Cuts at: 2177 4810 6522 2177
Size: 2633 1712 2217
Fragments arranged by size:
2633 2217 1712
SfcI C'TryA_G
Cuts at: 826 1045 2167 3910 5045 5726 5917 826
Size: 219 1122 1743 1135 681 191 1471
Fragments arranged by size:
1743 1471 1135 1122 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3804 3804
Size: 6562
SfoI GGC'GCC
Cuts at: 3625 3625
Size: 6562
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6562
SmaI CCC'GGG
Cuts at: 1872 3582 3889 1872
Size: 1710 307 4545
Fragments arranged by size:
4545 1710 307
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3308 4666 5567 5829 820
Size: 197 926 1365 1358 901 262 1553
Fragments arranged by size:
1553 1365 1358 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4353 493
Size: 3860 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6562
SphI G_CATG'C
Cuts at: 1132 3920 1132
Size: 2788 3774
SspI AAT'ATT
Cuts at: 5 52 4486 5
Size: 47 4434 2081
Fragments arranged by size:
4434 2081 47
StuI AGG'CCT
Cuts at: 1517 2600 3039 3089 1517
Size: 1083 439 50 4990
Fragments arranged by size:
4990 1083 439 50
StyI C'CwwG_G
Cuts at: 513 1065 2004 2715 2826 3084 3618 3785 4001
Size: 552 939 711 111 258 534 167 216
Cuts at: 4001 513
Size: 3074
Fragments arranged by size:
3074 939 711 552 534 258 216 167
111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3041 4589 4606 4759 4944 5389 325
Size: 1255 1461 1548 17 153 185 445 1498
Fragments arranged by size:
1548 1498 1461 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 4808 6520
Size: 274 80 33 51 1443 198 2633 1712
Cuts at: 6520 96
Size: 138
Fragments arranged by size:
2633 1712 1443 274 198 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2339 2371 3824 3856 6522 6554 2339
Size: 32 1453 32 2666 32 2347
Fragments arranged by size:
2666 2347 1453 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2240 1316
Size: 924 5638
XbaI T'CTAG_A
Cuts at: 3898 3898
Size: 6562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3604 3604
Size: 6562
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6562
XmaI C'CCGG_G
Cuts at: 1870 3580 3887 1870
Size: 1710 307 4545
Fragments arranged by size:
4545 1710 307
XmnI GAAnn'nnTTC
Cuts at: 2189 3677 4691 2189
Size: 1488 1014 4060
Fragments arranged by size:
4060 1488 1014
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4370 276
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV NruI NsiI PacI
PspXI PsrI RsrII SexAI SrfI SwaI