Restriction map for
pFN21AE4166
(Circular) (Six-base) MAPSORT of: pFN21AE4166.seq Check: 9077 from: 1 to: 6559
pFN21AE4166 6559 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2060 2142 2256 3123
Size: 53 83 186 936 524 82 114 867
Cuts at: 3123 4369 278
Size: 1246 2468
Fragments arranged by size:
2468 1246 936 867 524 186 114 83
82 53
AccI GT'mk_AC
Cuts at: 1937 3525 3902 1937
Size: 1588 377 4594
Fragments arranged by size:
4594 1588 377
Acc65I G'GTAC_C
Cuts at: 3880 3880
Size: 6559
AclI AA'CG_TT
Cuts at: 1563 4686 5059 1563
Size: 3123 373 3063
Fragments arranged by size:
3123 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2566 4622 6006 6274 1632
Size: 934 2056 1384 268 1917
Fragments arranged by size:
2056 1917 1384 934 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6362
AflIII A'CryG_T
Cuts at: 1203 5458 6435 1203
Size: 4255 977 1327
Fragments arranged by size:
4255 1327 977
AgeI A'CCGG_T
Cuts at: 5390 5390
Size: 6559
AhdI GACnn_n'nnGTC
Cuts at: 2371 3499 5288 2371
Size: 1128 1789 3642
Fragments arranged by size:
3642 1789 1128
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6559
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6559
AlwNI CAG_nnn'CTG
Cuts at: 2569 2602 2680 4302 5874 2569
Size: 33 78 1622 1572 3254
Fragments arranged by size:
3254 1622 1572 78 33
ApaI G_GGCC'C
Cuts at: 2331 3032 3277 2331
Size: 701 245 5613
Fragments arranged by size:
5613 701 245
ApaLI G'TGCA_C
Cuts at: 4616 5772 4616
Size: 1156 5403
ApoI r'AATT_y
Cuts at: 1493 3868 4094 4246 6355 1493
Size: 2375 226 152 2109 1697
Fragments arranged by size:
2375 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5113 160
Size: 872 4081 1606
Fragments arranged by size:
4081 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6559
AvaI C'yCGr_G
Cuts at: 1870 1943 2093 2420 3032 3884 1870
Size: 73 150 327 612 852 4545
Fragments arranged by size:
4545 852 612 327 150 73
AvrII C'CTAG_G
Cuts at: 3142 3142
Size: 6559
BamHI G'GATC_C
Cuts at: 2295 3889 4256 2295
Size: 1594 367 4598
Fragments arranged by size:
4598 1594 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2155 2335 2658 2969 3330
Size: 325 217 627 368 180 323 311 361
Cuts at: 3330 3880 5335 618
Size: 550 1455 1842
Fragments arranged by size:
1842 1455 627 550 368 361 325 323
311 217 180
BanII G_rGCy'C
Cuts at: 1391 2331 2384 2631 2907 3032 3039 3277 3690
Size: 940 53 247 276 125 7 238 413
Cuts at: 3690 3878 1391
Size: 188 4072
Fragments arranged by size:
4072 940 413 276 247 238 188 125
53 7
BbeI G_GCGC'C
Cuts at: 2973 2973
Size: 6559
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2060 2699 928
Size: 971 161 639 4788
Fragments arranged by size:
4788 971 639 161
BbvCI CC'TCA_GC
Cuts at: 3821 3821
Size: 6559
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4750 4784 1903
Size: 34 2813 34 3678
Fragments arranged by size:
3678 2813 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3077 4451 5661 3077
Size: 1374 1210 3975
Fragments arranged by size:
3975 1374 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6559
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2495 2675 3722
Size: 107 122 71 631 605 823 180 1047
Cuts at: 3722 3809 5170 136
Size: 87 1361 1525
Fragments arranged by size:
1525 1361 1047 823 631 605 180 122
107 87 71
BglII A'GATC_T
Cuts at: 6554 6554
Size: 6559
BlpI GC'TnA_GC
Cuts at: 3976 3976
Size: 6559
Bme1580I G_kGCm'C
Cuts at: 1790 2160 2331 2340 2661 2886 3032 3277 3333
Size: 370 171 9 321 225 146 245 56
Cuts at: 3333 3425 4620 5776 1790
Size: 92 1195 1156 2573
Fragments arranged by size:
2573 1195 1156 370 321 245 225 171
146 92 56 9
BmgBI CAC'GTC
Cuts at: 1318 2017 2689 1318
Size: 699 672 5188
Fragments arranged by size:
5188 699 672
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2042 4290 5248
Size: 416 53 83 399 24 625 2248 958
Cuts at: 5248 442
Size: 1753
Fragments arranged by size:
2248 1753 958 625 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2702 2864 5219 1239
Size: 1463 162 2355 2579
Fragments arranged by size:
2579 2355 1463 162
Bpu10I CC'TnA_GC
Cuts at: 3821 3821
Size: 6559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4684 5549 5847 4684
Size: 865 298 5396
Fragments arranged by size:
5396 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2423 2792 5222 882
Size: 617 924 369 2430 2219
Fragments arranged by size:
2430 2219 924 617 369
BsaAI yAC'GTr
Cuts at: 493 1204 2881 4350 493
Size: 711 1677 1469 2702
Fragments arranged by size:
2702 1677 1469 711
BsaBI GATnn'nnATC
Cuts at: 2126 6553 2126
Size: 4427 2132
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2057 2139 2253 2970
Size: 53 83 186 936 524 82 114 717
Cuts at: 2970 3120 3453 4366 4748 5383 275
Size: 150 333 913 382 635 1451
Fragments arranged by size:
1451 936 913 717 635 524 382 333
186 150 114 83 82 53
BsaWI w'CCGG_w
Cuts at: 3123 4992 5390 5664 5811 6222 3123
Size: 1869 398 274 147 411 3460
Fragments arranged by size:
3460 1869 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2849 2879 1178
Size: 30 165 30 1446 30 4858
Fragments arranged by size:
4858 1446 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2111 2132 2216 2228 2276 3562 1186
Size: 913 12 21 84 12 48 1286 4183
Fragments arranged by size:
4183 1286 913 84 48 21 12 12
BseYI C'CCAG_C
Cuts at: 3013 3679 3689 3708 3787 5762 6258 3013
Size: 666 10 19 79 1975 496 3314
Fragments arranged by size:
3314 1975 666 496 79 19 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2725 2951 3062 3398 1163
Size: 573 989 226 111 336 4324
Fragments arranged by size:
4324 989 573 336 226 111
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2375 2791 3067 3319 3624 4009
Size: 275 186 374 416 276 252 305 385
Cuts at: 4009 4770 5798 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 416 385 374 305 276
275 252 186
BsiHKAI G_wGCw'C
Cuts at: 2384 2400 2631 2907 3039 4620 4705 5776 2384
Size: 16 231 276 132 1581 85 1071 3167
Fragments arranged by size:
3167 1581 1071 276 231 132 85 16
BsmI GAATG_Cn'
Cuts at: 4070 4163 4070
Size: 93 6466
BsmBI CGTCTCn'nnnn_
Cuts at: 2260 2260
Size: 6559
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2331 2340 2384 2400 2631 2661
Size: 399 370 171 9 44 16 231 30
Cuts at: 2661 2886 2907 3032 3039 3277 3333 3425 3690
Size: 225 21 125 7 238 56 92 265
Cuts at: 3690 3878 4620 4705 5776 1391
Size: 188 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 370 265 238 231
225 188 171 125 92 85 56 44
30 21 16 9 7
BspEI T'CCGG_A
Cuts at: 3123 6222 3123
Size: 3099 3460
BspHI T'CATG_A
Cuts at: 2406 4448 6178 2406
Size: 2042 1730 2787
Fragments arranged by size:
2787 2042 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2376 2441 3370 3594 3914 844
Size: 1532 65 929 224 320 3489
Fragments arranged by size:
3489 1532 929 320 224 65
BsrBI CCG'CTC
Cuts at: 1394 1823 3653 4446 1394
Size: 429 1830 793 3507
Fragments arranged by size:
3507 1830 793 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5054 5228 65
Size: 1183 3806 174 1396
Fragments arranged by size:
3806 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2736 2790 3062 3066 3619 3642 5203
Size: 110 926 54 272 4 553 23 1561
Cuts at: 5203 5390 1700
Size: 187 2869
Fragments arranged by size:
2869 1561 926 553 272 187 110 54
23 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6559
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6559
BssSI C'ACGA_G
Cuts at: 2795 3204 3340 4619 5631 2795
Size: 409 136 1279 1012 3723
Fragments arranged by size:
3723 1279 1012 409 136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3515 794
Size: 2721 3838
BstBI TT'CG_AA
Cuts at: 2284 5363 2284
Size: 3079 3480
BstEII G'GTnAC_C
Cuts at: 1184 3106 1184
Size: 1922 4637
BstXI CCAn_nnnn'nTGG
Cuts at: 3448 3448
Size: 6559
BstYI r'GATC_y
Cuts at: 1972 2217 2295 3889 4256 4641 4658 6110 6196
Size: 245 78 1594 367 385 17 1452 86
Cuts at: 6196 6208 6554 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1594 1452 385 367 346 245 86
78 17 12
Bsu36I CC'TnA_GG
Cuts at: 2459 2532 2459
Size: 73 6486
BtgI C'CryG_G
Cuts at: 513 1065 2133 2385 2982 3277 3319 3720 3807
Size: 552 1068 252 597 295 42 401 87
Cuts at: 3807 513
Size: 3265
Fragments arranged by size:
3265 1068 597 552 401 295 252 87
42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2850 3429 487
Size: 683 56 1624 579 3617
Fragments arranged by size:
3617 1624 683 579 56
BtsI GCAGTG_nn'
Cuts at: 3496 4075 4869 4889 3496
Size: 579 794 20 5166
Fragments arranged by size:
5166 794 579 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6559
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6524
DraI TTT'AAA
Cuts at: 3863 3863
Size: 6559
DraIII CAC_nnn'GTG
Cuts at: 2170 3515 2170
Size: 1345 5214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5566 809
Size: 507 4250 1802
Fragments arranged by size:
4250 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2448 2788 3064
Size: 54 1425 50 275 18 618 340 276
Cuts at: 3064 3316 3438 3621 3723 3810 4006 4895 6524
Size: 252 122 183 102 87 196 889 1629
Cuts at: 6524 8
Size: 43
Fragments arranged by size:
1629 1425 889 618 340 276 275 252
196 183 122 102 87 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2788 3064 3316 3621 4006 1537
Size: 275 976 276 252 305 385 4090
Fragments arranged by size:
4090 976 385 305 276 275 252
EarI CTCTTCn'nnn_
Cuts at: 1353 2619 2765 2780 3206 3769 3845 4489 1353
Size: 1266 146 15 426 563 76 644 3423
Fragments arranged by size:
3423 1266 644 563 426 146 76 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5146 5520 5666 387
Size: 1064 138 3557 374 146 1280
Fragments arranged by size:
3557 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2382 2629 2905 3037 2382
Size: 247 276 132 5904
Fragments arranged by size:
5904 276 247 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2566 2702 2864 4622 5219 6006 6274
Size: 393 934 136 162 1758 597 787 268
Cuts at: 6274 1239
Size: 1524
Fragments arranged by size:
1758 1524 934 787 597 393 268 162
136
EcoNI CCTnn'n_nnAGG
Cuts at: 2606 3260 3413 2606
Size: 654 153 5752
Fragments arranged by size:
5752 654 153
EcoO109I rG'GnC_Cy
Cuts at: 2101 2327 2599 2677 3028 3029 3273 3841 2101
Size: 226 272 78 351 1 244 568 4819
Fragments arranged by size:
4819 568 351 272 244 226 78 1
EcoRI G'AATT_C
Cuts at: 3868 4246 3868
Size: 378 6181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3313 3313
Size: 6559
FseI GG_CCGG'CC
Cuts at: 3066 3066
Size: 6559
FspI TGC'GCA
Cuts at: 2021 5065 2021
Size: 3044 3515
FspAI rTGC'GCAy
Cuts at: 2021 2021
Size: 6559
HaeII r_GCGC'y
Cuts at: 1678 2570 2615 2824 2973 3023 5706 1678
Size: 892 45 209 149 50 2683 2531
Fragments arranged by size:
2683 2531 892 209 149 50 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2357 2389 2849 2881 3485
Size: 32 135 32 785 32 460 32 604
Cuts at: 3485 3517 5200 5232 5274 5306 1373
Size: 32 1683 32 42 32 2626
Fragments arranged by size:
2626 1683 785 604 460 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3903 4149 669
Size: 988 281 1965 246 3079
Fragments arranged by size:
3079 1965 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6559
HpaI GTT'AAC
Cuts at: 4149 4149
Size: 6559
KasI G'GCGC_C
Cuts at: 2969 2969
Size: 6559
KpnI G_GTAC'C
Cuts at: 3884 3884
Size: 6559
MfeI C'AATT_G
Cuts at: 4158 4158
Size: 6559
MluI A'CGCG_T
Cuts at: 6435 6435
Size: 6559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5673 5857 1305
Size: 4368 184 2007
Fragments arranged by size:
4368 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3440 10
Size: 54 1425 343 1608 3129
Fragments arranged by size:
3129 1608 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 4517 4876 5035 518
Size: 648 1182 2169 359 159 2042
Fragments arranged by size:
2169 2042 1182 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2135 3244 3321 3975 4652 5800 6045 1606
Size: 529 1109 77 654 677 1148 245 2120
Fragments arranged by size:
2120 1148 1109 677 654 529 245 77
NaeI GCC'GGC
Cuts at: 2792 3064 2792
Size: 272 6287
NarI GG'CG_CC
Cuts at: 2970 2970
Size: 6559
NcoI C'CATG_G
Cuts at: 513 1065 2385 3720 513
Size: 552 1320 1335 3352
Fragments arranged by size:
3352 1335 1320 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6559
NgoMIV G'CCGG_C
Cuts at: 2790 3062 2790
Size: 272 6287
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6559
NotI GC'GGCC_GC
Cuts at: 4006 4006
Size: 6559
NspI r_CATG'y
Cuts at: 1132 1229 3917 5462 1132
Size: 97 2688 1545 2229
Fragments arranged by size:
2688 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2331 3024 1348
Size: 983 693 4883
Fragments arranged by size:
4883 983 693
PciI A'CATG_T
Cuts at: 5458 5458
Size: 6559
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2170 3541 1108
Size: 1062 1371 4126
Fragments arranged by size:
4126 1371 1062
PmeI GTTT'AAAC
Cuts at: 3863 3863
Size: 6559
PmlI CAC'GTG
Cuts at: 2881 2881
Size: 6559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4628 4660 6141 6173 4628
Size: 32 1481 32 5014
Fragments arranged by size:
5014 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2101 2599 3841 2101
Size: 498 1242 4819
Fragments arranged by size:
4819 1242 498
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6559
PsiI TTA'TAA
Cuts at: 4129 4129
Size: 6559
PspOMI G'GGCC_C
Cuts at: 2327 3028 3273 2327
Size: 701 245 5613
Fragments arranged by size:
5613 701 245
PstI C_TGCA'G
Cuts at: 830 2897 3306 3911 830
Size: 2067 409 605 3478
Fragments arranged by size:
3478 2067 605 409
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6559
SacI G_AGCT'C
Cuts at: 2384 2631 2907 3039 2384
Size: 247 276 132 5904
Fragments arranged by size:
5904 276 247 132
SacII CC_GC'GG
Cuts at: 2136 3322 2136
Size: 1186 5373
SalI G'TCGA_C
Cuts at: 1936 3901 1936
Size: 1965 4594
SapI GCTCTTCn'nnn_
Cuts at: 2619 2780 3845 2619
Size: 161 1065 5333
Fragments arranged by size:
5333 1065 161
SbfI CC_TGCA'GG
Cuts at: 3306 3911 3306
Size: 605 5954
ScaI AGT'ACT
Cuts at: 3401 4807 6519 3401
Size: 1406 1712 3441
Fragments arranged by size:
3441 1712 1406
SfcI C'TryA_G
Cuts at: 826 1045 2830 2893 3302 3835 3907 5042 5723
Size: 219 1785 63 409 533 72 1135 681
Cuts at: 5723 5914 826
Size: 191 1471
Fragments arranged by size:
1785 1471 1135 681 533 409 219 191
72 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 2675 3809 2675
Size: 1134 5425
SfoI GGC'GCC
Cuts at: 2971 2971
Size: 6559
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6559
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6559
SmaI CCC'GGG
Cuts at: 1872 3886 1872
Size: 2014 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4663 5564 5826 820
Size: 197 926 2720 901 262 1553
Fragments arranged by size:
2720 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4350 493
Size: 3857 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6559
SphI G_CATG'C
Cuts at: 1132 3917 1132
Size: 2785 3774
SspI AAT'ATT
Cuts at: 5 52 4483 5
Size: 47 4431 2081
Fragments arranged by size:
4431 2081 47
StuI AGG'CCT
Cuts at: 1517 3308 1517
Size: 1791 4768
StyI C'CwwG_G
Cuts at: 513 1065 2385 2914 3133 3142 3576 3720 3998
Size: 552 1320 529 219 9 434 144 278
Cuts at: 3998 513
Size: 3074
Fragments arranged by size:
3074 1320 552 529 434 278 219 144
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2177 3722 4586 4603 4756 4941 5386
Size: 1255 597 1545 864 17 153 185 445
Cuts at: 5386 325
Size: 1498
Fragments arranged by size:
1545 1498 1255 864 597 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3399 4805 6517
Size: 274 80 33 51 1443 1422 1406 1712
Cuts at: 6517 96
Size: 138
Fragments arranged by size:
1712 1443 1422 1406 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3033 3065 3726 3758 6519 6551 3033
Size: 32 661 32 2761 32 3041
Fragments arranged by size:
3041 2761 661 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6559
XbaI T'CTAG_A
Cuts at: 3895 3895
Size: 6559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 2347 3021 2167
Size: 180 674 5705
Fragments arranged by size:
5705 674 180
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6559
XmaI C'CCGG_G
Cuts at: 1870 3884 1870
Size: 2014 4545
XmnI GAAnn'nnTTC
Cuts at: 4688 4688
Size: 6559
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2058 2140 2254 3121
Size: 53 83 186 936 524 82 114 867
Cuts at: 3121 4367 276
Size: 1246 2468
Fragments arranged by size:
2468 1246 936 867 524 186 114 83
82 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BfrBI BplI BsiWI
BstZ17I EcoRV NruI NsiI PacI PfoI PspXI PsrI
PvuII RsrII SanDI SexAI SrfI SwaI