Restriction map for
pFN21AE4165
(Circular) (Six-base) MAPSORT of: pFN21AE4165.seq Check: 1183 from: 1 to: 6550
pFN21AE4165 6550 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3123 3123
Size: 6550
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2313 4360 278
Size: 53 83 186 936 777 2047 2468
Fragments arranged by size:
2468 2047 936 777 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2994 3893 1937
Size: 1057 899 4594
Fragments arranged by size:
4594 1057 899
Acc65I G'GTAC_C
Cuts at: 3871 3871
Size: 6550
AclI AA'CG_TT
Cuts at: 1563 4677 5050 1563
Size: 3114 373 3063
Fragments arranged by size:
3114 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2251 2342 2492 2512 3362 4613 5997 6265
Size: 619 91 150 20 850 1251 1384 268
Cuts at: 6265 1632
Size: 1917
Fragments arranged by size:
1917 1384 1251 850 619 268 150 91
20
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6353
AflIII A'CryG_T
Cuts at: 1203 3063 5449 6426 1203
Size: 1860 2386 977 1327
Fragments arranged by size:
2386 1860 1327 977
AgeI A'CCGG_T
Cuts at: 5381 5381
Size: 6550
AhdI GACnn_n'nnGTC
Cuts at: 5279 5279
Size: 6550
AleI CACnn'nnGTG
Cuts at: 1166 2912 3068 1166
Size: 1746 156 4648
Fragments arranged by size:
4648 1746 156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2672 2682 2930 1184
Size: 1488 10 248 4804
Fragments arranged by size:
4804 1488 248 10
AlwNI CAG_nnn'CTG
Cuts at: 2161 2440 2806 2845 2872 3115 4293 5865 2161
Size: 279 366 39 27 243 1178 1572 2846
Fragments arranged by size:
2846 1572 1178 366 279 243 39 27
ApaI G_GGCC'C
Cuts at: 2066 2072 2110 2066
Size: 6 38 6506
Fragments arranged by size:
6506 38 6
ApaLI G'TGCA_C
Cuts at: 2116 4607 5763 2116
Size: 2491 1156 2903
Fragments arranged by size:
2903 2491 1156
ApoI r'AATT_y
Cuts at: 1493 3859 4085 4237 6346 1493
Size: 2366 226 152 2109 1697
Fragments arranged by size:
2366 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5104 160
Size: 872 4072 1606
Fragments arranged by size:
4072 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6550
AvaI C'yCGr_G
Cuts at: 1870 1943 2065 2072 3397 3542 3831 3875 1870
Size: 73 122 7 1325 145 289 44 4545
Fragments arranged by size:
4545 1325 289 145 122 73 44 7
BamHI G'GATC_C
Cuts at: 3880 4247 3880
Size: 367 6183
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2128 3478 3518 3871 5326
Size: 325 217 627 341 1350 40 353 1455
Cuts at: 5326 618
Size: 1842
Fragments arranged by size:
1842 1455 1350 627 353 341 325 217
40
BanII G_rGCy'C
Cuts at: 1391 2066 2072 2110 3019 3036 3635 3869 1391
Size: 675 6 38 909 17 599 234 4072
Fragments arranged by size:
4072 909 675 599 234 38 17 6
BbeI G_GCGC'C
Cuts at: 3482 3482
Size: 6550
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2626 928
Size: 971 727 4852
Fragments arranged by size:
4852 971 727
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4741 4775 1903
Size: 34 2804 34 3678
Fragments arranged by size:
3678 2804 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4442 5652 4442
Size: 1210 5340
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6550
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2881 5161 136
Size: 107 122 71 631 605 1209 2280 1525
Fragments arranged by size:
2280 1525 1209 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3684 6545 3684
Size: 2861 3689
BlpI GC'TnA_GC
Cuts at: 3967 3967
Size: 6550
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2072 2110 2120 2931 4611 5767 1790
Size: 276 6 38 10 811 1680 1156 2573
Fragments arranged by size:
2573 1680 1156 811 276 38 10 6
BmgBI CAC'GTC
Cuts at: 1318 2410 2827 3580 1318
Size: 1092 417 753 4288
Fragments arranged by size:
4288 1092 753 417
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4281 5239 442
Size: 416 53 83 399 24 2864 958 1753
Fragments arranged by size:
2864 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2642 2870 3695 5210 1239
Size: 1403 228 825 1515 2579
Fragments arranged by size:
2579 1515 1403 825 228
Bpu10I CC'TnA_GC
Cuts at: 2178 3006 2178
Size: 828 5722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2096 4675 5540 5838 2096
Size: 2579 865 298 2808
Fragments arranged by size:
2808 2579 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2486 5213 882
Size: 617 987 2727 2219
Fragments arranged by size:
2727 2219 987 617
BsaAI yAC'GTr
Cuts at: 493 1204 2743 3066 3097 3562 4341 493
Size: 711 1539 323 31 465 779 2702
Fragments arranged by size:
2702 1539 779 711 465 323 31
BsaBI GATnn'nnATC
Cuts at: 3689 6544 3689
Size: 2855 3695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2310 3147 3479 3712
Size: 53 83 186 936 777 837 332 233
Cuts at: 3712 4357 4739 5374 275
Size: 645 382 635 1451
Fragments arranged by size:
1451 936 837 777 645 635 382 332
233 186 83 53
BsaWI w'CCGG_w
Cuts at: 4983 5381 5655 5802 6213 4983
Size: 398 274 147 411 5320
Fragments arranged by size:
5320 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6325
Fragments arranged by size:
6325 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2180 2477 2609 2612 1186
Size: 994 297 132 3 5124
Fragments arranged by size:
5124 994 297 132 3
BseYI C'CCAG_C
Cuts at: 2132 2413 3365 5753 6249 2132
Size: 281 952 2388 496 2433
Fragments arranged by size:
2433 2388 952 496 281
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2214 2423 2819 3422 3764 1163
Size: 573 478 209 396 603 342 3949
Fragments arranged by size:
3949 603 573 478 396 342 209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2094 2424 2694 2729 2974 3265
Size: 275 186 93 330 270 35 245 291
Cuts at: 3265 3529 4000 4761 5789 1540
Size: 264 471 761 1028 2301
Fragments arranged by size:
2301 1028 761 471 330 291 275 270
264 245 186 93 35
BsiHKAI G_wGCw'C
Cuts at: 2120 2748 3036 4611 4696 5767 2120
Size: 628 288 1575 85 1071 2903
Fragments arranged by size:
2903 1575 1071 628 288 85
BsmI GAATG_Cn'
Cuts at: 4061 4154 4061
Size: 93 6457
BsmBI CGTCTCn'nnnn_
Cuts at: 3602 3719 3602
Size: 117 6433
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2072 2110 2120 2748 2931 3019
Size: 399 276 6 38 10 628 183 88
Cuts at: 3019 3036 3635 3869 4611 4696 5767 1391
Size: 17 599 234 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 628 599 399 276 234
183 88 85 38 17 10 6
BspEI T'CCGG_A
Cuts at: 6213 6213
Size: 6550
BspHI T'CATG_A
Cuts at: 4439 6169 4439
Size: 1730 4820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2504 3123 3786 3905 844
Size: 1660 619 663 119 3489
Fragments arranged by size:
3489 1660 663 619 119
BsrBI CCG'CTC
Cuts at: 1394 1823 2767 2976 3298 3772 4437 1394
Size: 429 944 209 322 474 665 3507
Fragments arranged by size:
3507 944 665 474 429 322 209
BsrDI GCAATG_nn'
Cuts at: 65 1248 3322 5045 5219 65
Size: 1183 2074 1723 174 1396
Fragments arranged by size:
2074 1723 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2282 2652 3275 3521 3644 3659 5194
Size: 110 472 370 623 246 123 15 1535
Cuts at: 5194 5381 1700
Size: 187 2869
Fragments arranged by size:
2869 1535 623 472 370 246 187 123
110 15
BsrGI T'GTAC_A
Cuts at: 96 3744 96
Size: 3648 2902
BssHII G'CGCG_C
Cuts at: 1925 2731 1925
Size: 806 5744
BssSI C'ACGA_G
Cuts at: 2888 4610 5622 2888
Size: 1722 1012 3816
Fragments arranged by size:
3816 1722 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2410 2440 2581 2893 3115 3199 3810 794
Size: 1616 30 141 312 222 84 611 3534
Fragments arranged by size:
3534 1616 611 312 222 141 84 30
BstBI TT'CG_AA
Cuts at: 5354 5354
Size: 6550
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6550
BstXI CCAn_nnnn'nTGG
Cuts at: 2488 2488
Size: 6550
BstYI r'GATC_y
Cuts at: 1972 3411 3684 3880 4247 4632 4649 6101 6187
Size: 1439 273 196 367 385 17 1452 86
Cuts at: 6187 6199 6545 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1439 385 367 346 273 196
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3376 3376
Size: 6550
BtgI C'CryG_G
Cuts at: 513 1065 2004 2338 2445 3255 3291 3453 3510
Size: 552 939 334 107 810 36 162 57
Cuts at: 3510 513
Size: 3553
Fragments arranged by size:
3553 939 810 552 334 162 107 57
36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5811
Fragments arranged by size:
5811 683 56
BtsI GCAGTG_nn'
Cuts at: 3147 3439 4066 4860 4880 3147
Size: 292 627 794 20 4817
Fragments arranged by size:
4817 794 627 292 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6550
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6515
DraI TTT'AAA
Cuts at: 3854 3854
Size: 6550
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2978 5557 809
Size: 507 1662 2579 1802
Fragments arranged by size:
2579 1802 1662 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2163 2421
Size: 54 1425 50 275 18 261 72 258
Cuts at: 2421 2478 2509 2517 2691 2726 3072 3099 3262
Size: 57 31 8 174 35 346 27 163
Cuts at: 3262 3456 3526 3646 3997 4886 6515 8
Size: 194 70 120 351 889 1629 43
Fragments arranged by size:
1629 1425 889 351 346 275 261 258
194 174 163 120 72 70 57 54
50 43 35 31 27 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2091 2421 2691 2726 3262 3526 3997
Size: 275 279 330 270 35 536 264 471
Cuts at: 3997 1537
Size: 4090
Fragments arranged by size:
4090 536 471 330 279 275 270 264
35
EarI CTCTTCn'nnn_
Cuts at: 1353 4480 1353
Size: 3127 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5137 5511 5657 387
Size: 1064 138 435 3113 374 146 1280
Fragments arranged by size:
3113 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 3034 3034
Size: 6550
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2251 2342 2492 2512 2642 2870 3362
Size: 393 619 91 150 20 130 228 492
Cuts at: 3362 3695 4613 5210 5997 6265 1239
Size: 333 918 597 787 268 1524
Fragments arranged by size:
1524 918 787 619 597 492 393 333
268 228 150 130 91 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 2153
Size: 6550
EcoO109I rG'GnC_Cy
Cuts at: 2069 2106 2107 2158 3433 3775 2069
Size: 37 1 51 1275 342 4844
Fragments arranged by size:
4844 1275 342 51 37 1
EcoRI G'AATT_C
Cuts at: 3859 4237 3859
Size: 378 6172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 6550
FseI GG_CCGG'CC
Cuts at: 3648 3648
Size: 6550
FspI TGC'GCA
Cuts at: 5056 5056
Size: 6550
HaeII r_GCGC'y
Cuts at: 1678 2052 2080 2376 2629 3349 3482 5697 1678
Size: 374 28 296 253 720 133 2215 2531
Fragments arranged by size:
2531 2215 720 374 296 253 133 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2882 2884 2914 2915 2916
Size: 32 135 32 1310 2 30 1 1
Cuts at: 2916 2947 5191 5223 5265 5297 1373
Size: 31 2244 32 42 32 2626
Fragments arranged by size:
2626 2244 1310 135 42 32 32 32
32 31 30 2 1 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3894 4140 669
Size: 988 281 1956 246 3079
Fragments arranged by size:
3079 1956 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6550
HpaI GTT'AAC
Cuts at: 4140 4140
Size: 6550
KasI G'GCGC_C
Cuts at: 3478 3478
Size: 6550
KpnI G_GTAC'C
Cuts at: 3875 3875
Size: 6550
MfeI C'AATT_G
Cuts at: 4149 4149
Size: 6550
MluI A'CGCG_T
Cuts at: 6426 6426
Size: 6550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5664 5848 1305
Size: 4359 184 2007
Fragments arranged by size:
4359 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2165 2519 10
Size: 54 1425 343 333 354 4041
Fragments arranged by size:
4041 1425 354 343 333 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 2816 2912 3041 3068 4508 4867
Size: 648 1320 330 96 129 27 1440 359
Cuts at: 4867 5026 518
Size: 159 2042
Fragments arranged by size:
2042 1440 1320 648 359 330 159 129
96 27
MspA1I CmG'CkG
Cuts at: 1606 2028 2700 3001 3129 3226 3570 3966 4643
Size: 422 672 301 128 97 344 396 677
Cuts at: 4643 5791 6036 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 672 422 396 344 301
245 128 97
NaeI GCC'GGC
Cuts at: 3277 3523 3646 3661 3277
Size: 246 123 15 6166
Fragments arranged by size:
6166 246 123 15
NarI GG'CG_CC
Cuts at: 3479 3479
Size: 6550
NcoI C'CATG_G
Cuts at: 513 1065 2004 3510 513
Size: 552 939 1506 3553
Fragments arranged by size:
3553 1506 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6550
NgoMIV G'CCGG_C
Cuts at: 3275 3521 3644 3659 3275
Size: 246 123 15 6166
Fragments arranged by size:
6166 246 123 15
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6550
NotI GC'GGCC_GC
Cuts at: 2726 3262 3526 3997 2726
Size: 536 264 471 5279
Fragments arranged by size:
5279 536 471 264
NspI r_CATG'y
Cuts at: 1132 1229 2293 3908 5453 1132
Size: 97 1064 1615 1545 2229
Fragments arranged by size:
2229 1615 1545 1064 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6550
PciI A'CATG_T
Cuts at: 5449 5449
Size: 6550
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6550
PfoI T'CCnGG_A
Cuts at: 3786 3786
Size: 6550
PmeI GTTT'AAAC
Cuts at: 3854 3854
Size: 6550
PmlI CAC'GTG
Cuts at: 2743 3066 3562 2743
Size: 323 496 5731
Fragments arranged by size:
5731 496 323
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4619 4651 6132 6164 4619
Size: 32 1481 32 5005
Fragments arranged by size:
5005 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3775 3775
Size: 6550
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6550
PsiI TTA'TAA
Cuts at: 4120 4120
Size: 6550
PspOMI G'GGCC_C
Cuts at: 2062 2068 2106 2062
Size: 6 38 6506
Fragments arranged by size:
6506 38 6
PstI C_TGCA'G
Cuts at: 830 2157 2258 2586 2646 2661 2709 3059 3120
Size: 1327 101 328 60 15 48 350 61
Cuts at: 3120 3195 3902 830
Size: 75 707 3478
Fragments arranged by size:
3478 1327 707 350 328 101 75 61
60 48 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6550
PvuII CAG'CTG
Cuts at: 3001 3226 3001
Size: 225 6325
SacI G_AGCT'C
Cuts at: 3036 3036
Size: 6550
SalI G'TCGA_C
Cuts at: 1936 3892 1936
Size: 1956 4594
SbfI CC_TGCA'GG
Cuts at: 2157 3195 3902 2157
Size: 1038 707 4805
Fragments arranged by size:
4805 1038 707
ScaI AGT'ACT
Cuts at: 3596 4798 6510 3596
Size: 1202 1712 3636
Fragments arranged by size:
3636 1712 1202
SexAI A'CCwGG_T
Cuts at: 2865 2865
Size: 6550
SfcI C'TryA_G
Cuts at: 826 1045 2153 2254 2368 2582 2642 2657 2705
Size: 219 1108 101 114 214 60 15 48
Cuts at: 2705 3055 3116 3191 3898 5033 5714 5905 826
Size: 350 61 75 707 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 707 681 350 219 214
191 114 101 75 61 60 48 15
SfoI GGC'GCC
Cuts at: 3480 3480
Size: 6550
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6550
SgrAI Cr'CCGG_yG
Cuts at: 1700 2282 1700
Size: 582 5968
SmaI CCC'GGG
Cuts at: 1872 2067 3833 3877 1872
Size: 195 1766 44 4545
Fragments arranged by size:
4545 1766 195 44
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2111 4654 5555 5817 820
Size: 197 926 168 2543 901 262 1553
Fragments arranged by size:
2543 1553 926 901 262 197 168
SnaBI TAC'GTA
Cuts at: 493 4341 493
Size: 3848 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6550
SphI G_CATG'C
Cuts at: 1132 2293 3908 1132
Size: 1161 1615 3774
Fragments arranged by size:
3774 1615 1161
SrfI GCCC'GGGC
Cuts at: 2067 2067
Size: 6550
SspI AAT'ATT
Cuts at: 5 52 4474 5
Size: 47 4422 2081
Fragments arranged by size:
4422 2081 47
StuI AGG'CCT
Cuts at: 1517 2222 2855 1517
Size: 705 633 5212
Fragments arranged by size:
5212 705 633
StyI C'CwwG_G
Cuts at: 513 1065 2004 3510 3989 513
Size: 552 939 1506 479 3074
Fragments arranged by size:
3074 1506 939 552 479
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2146 2968 3506 4577 4594 4747 4932
Size: 1255 566 822 538 1071 17 153 185
Cuts at: 4932 5377 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1071 822 566 538 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3594 3744 4796
Size: 274 80 33 51 1443 1617 150 1052
Cuts at: 4796 6508 96
Size: 1712 138
Fragments arranged by size:
1712 1617 1443 1052 274 150 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2816 2848 6510 6542 2816
Size: 32 3662 32 2824
Fragments arranged by size:
3662 2824 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6550
XbaI T'CTAG_A
Cuts at: 3886 3886
Size: 6550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2530 2530
Size: 6550
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6550
XmaI C'CCGG_G
Cuts at: 1870 2065 3831 3875 1870
Size: 195 1766 44 4545
Fragments arranged by size:
4545 1766 195 44
XmnI GAAnn'nnTTC
Cuts at: 4679 4679
Size: 6550
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2311 4358 276
Size: 53 83 186 936 777 2047 2468
Fragments arranged by size:
2468 2047 936 777 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I DraIII EcoRV FspAI NruI NsiI PacI
PspXI PsrI RsrII SacII SanDI SapI SfiI SwaI