Restriction map for
pFN21AE4158
(Circular) (Six-base) MAPSORT of: pFN21AE4158.seq Check: 1332 from: 1 to: 6301
pFN21AE4158 6301 bp
With 182 enzymes: SgfI *
March 28, 2012 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2985 4111 278
Size: 53 83 186 936 1449 1126 2468
Fragments arranged by size:
2468 1449 1126 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3644 1937
Size: 1707 4594
Acc65I G'GTAC_C
Cuts at: 2874 3622 2874
Size: 748 5553
AclI AA'CG_TT
Cuts at: 1563 3096 4428 4801 1563
Size: 1533 1332 373 3063
Fragments arranged by size:
3063 1533 1332 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4364 5748 6016 1632
Size: 2732 1384 268 1917
Fragments arranged by size:
2732 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 2373 2972 3113 5200 6177 1203
Size: 1170 599 141 2087 977 1327
Fragments arranged by size:
2087 1327 1170 977 599 141
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 5030 5030
Size: 6301
AleI CACnn'nnGTG
Cuts at: 1166 2466 1166
Size: 1300 5001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2829 1184
Size: 1645 4656
AlwNI CAG_nnn'CTG
Cuts at: 2197 2759 3019 3328 4044 5616 2197
Size: 562 260 309 716 1572 2882
Fragments arranged by size:
2882 1572 716 562 309 260
ApaLI G'TGCA_C
Cuts at: 2223 4358 5514 2223
Size: 2135 1156 3010
Fragments arranged by size:
3010 2135 1156
ApoI r'AATT_y
Cuts at: 1493 2819 3610 3836 3988 6097 1493
Size: 1326 791 226 152 2109 1697
Fragments arranged by size:
2109 1697 1326 791 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4855 160
Size: 872 3823 1606
Fragments arranged by size:
3823 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 3626 1870
Size: 73 147 1536 4545
Fragments arranged by size:
4545 1536 147 73
BamHI G'GATC_C
Cuts at: 3180 3631 3998 3180
Size: 451 367 5483
Fragments arranged by size:
5483 451 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2068 2298 2405 2874 3348
Size: 325 217 627 281 230 107 469 474
Cuts at: 3348 3622 5077 618
Size: 274 1455 1842
Fragments arranged by size:
1842 1455 627 474 469 325 281 274
230 217 107
BanII G_rGCy'C
Cuts at: 1391 2113 3518 3620 1391
Size: 722 1405 102 4072
Fragments arranged by size:
4072 1405 722 102
BbeI G_GCGC'C
Cuts at: 2072 2072
Size: 6301
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2934 928
Size: 971 1035 4295
Fragments arranged by size:
4295 1035 971
BbvCI CC'TCA_GC
Cuts at: 2726 3125 2726
Size: 399 5902
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4492 4526 1903
Size: 34 2555 34 3678
Fragments arranged by size:
3678 2555 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4193 5403 4193
Size: 1210 5091
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6301
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4912 136
Size: 107 122 71 631 605 3240 1525
Fragments arranged by size:
3240 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2902 6296 2902
Size: 3394 2907
BlpI GC'TnA_GC
Cuts at: 3718 3718
Size: 6301
Bme1580I G_kGCm'C
Cuts at: 1790 2227 2301 3351 4362 5518 1790
Size: 437 74 1050 1011 1156 2573
Fragments arranged by size:
2573 1156 1050 1011 437 74
BmgBI CAC'GTC
Cuts at: 1318 2241 1318
Size: 923 5378
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4032 4990 442
Size: 416 53 83 399 24 2615 958 1753
Fragments arranged by size:
2615 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6301
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2127 2127
Size: 6301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2441 2919 4961 1239
Size: 1202 478 2042 2579
Fragments arranged by size:
2579 2042 1202 478
Bpu10I CC'TnA_GC
Cuts at: 2726 3125 3233 2726
Size: 399 108 5794
Fragments arranged by size:
5794 399 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4426 5291 5589 4426
Size: 865 298 5138
Fragments arranged by size:
5138 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3244 4964 882
Size: 617 1745 1720 2219
Fragments arranged by size:
2219 1745 1720 617
BsaAI yAC'GTr
Cuts at: 493 1204 2533 3114 4092 493
Size: 711 1329 581 978 2702
Fragments arranged by size:
2702 1329 978 711 581
BsaBI GATnn'nnATC
Cuts at: 2488 6295 2488
Size: 3807 2494
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2069 2982 3073 3546
Size: 53 83 186 936 536 913 91 473
Cuts at: 3546 4108 4490 5125 275
Size: 562 382 635 1451
Fragments arranged by size:
1451 936 913 635 562 536 473 382
186 91 83 53
BsaWI w'CCGG_w
Cuts at: 2305 2669 4734 5132 5406 5553 5964 2305
Size: 364 2065 398 274 147 411 2642
Fragments arranged by size:
2642 2065 411 398 364 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6076
Fragments arranged by size:
6076 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2576 2807 1186
Size: 877 513 231 4680
Fragments arranged by size:
4680 877 513 231
BseYI C'CCAG_C
Cuts at: 3088 5504 6000 3088
Size: 2416 496 3389
Fragments arranged by size:
3389 2416 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3269 3377 1163
Size: 573 1533 108 4087
Fragments arranged by size:
4087 1533 573 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2120 3751 4512 5540 1540
Size: 275 186 119 1631 761 1028 2301
Fragments arranged by size:
2301 1631 1028 761 275 186 119
BsiHKAI G_wGCw'C
Cuts at: 2227 2700 4362 4447 5518 2227
Size: 473 1662 85 1071 3010
Fragments arranged by size:
3010 1662 1071 473 85
BsmI GAATG_Cn'
Cuts at: 2455 2583 3812 3905 2455
Size: 128 1229 93 4851
Fragments arranged by size:
4851 1229 128 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2989 2989
Size: 6301
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2113 2227 2301 2700 3351 3518 3620
Size: 399 323 114 74 399 651 167 102
Cuts at: 3620 4362 4447 5518 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 651 399 399 323 167
114 102 85 74
BspEI T'CCGG_A
Cuts at: 2305 5964 2305
Size: 3659 2642
BspHI T'CATG_A
Cuts at: 4190 5920 4190
Size: 1730 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2922 3656 844
Size: 2078 734 3489
Fragments arranged by size:
3489 2078 734
BsrBI CCG'CTC
Cuts at: 1394 1823 4188 1394
Size: 429 2365 3507
Fragments arranged by size:
3507 2365 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2475 4796 4970 65
Size: 1183 1227 2321 174 1396
Fragments arranged by size:
2321 1396 1227 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4945 5132 1700
Size: 110 3135 187 2869
Fragments arranged by size:
3135 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3010 96
Size: 2914 3387
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 4361 5373 4361
Size: 1012 5289
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3355 794
Size: 2561 3740
BstBI TT'CG_AA
Cuts at: 5105 5105
Size: 6301
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6301
BstXI CCAn_nnnn'nTGG
Cuts at: 3148 3148
Size: 6301
BstYI r'GATC_y
Cuts at: 1972 2902 3045 3180 3631 3998 4383 4400 5852
Size: 930 143 135 451 367 385 17 1452
Cuts at: 5852 5938 5950 6296 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 930 451 385 367 346 143
135 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2745 2956 3549 513
Size: 552 939 741 211 593 3265
Fragments arranged by size:
3265 939 741 593 552 211
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3190 487
Size: 683 56 1964 3598
Fragments arranged by size:
3598 1964 683 56
BtsI GCAGTG_nn'
Cuts at: 3202 3817 4611 4631 3202
Size: 615 794 20 4872
Fragments arranged by size:
4872 794 615 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6266
DraI TTT'AAA
Cuts at: 3605 3605
Size: 6301
DraIII CAC_nnn'GTG
Cuts at: 3355 3355
Size: 6301
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5308 809
Size: 507 3992 1802
Fragments arranged by size:
3992 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2117 2748 2988
Size: 54 1425 50 275 18 287 631 240
Cuts at: 2988 3393 3552 3748 4637 6266 8
Size: 405 159 196 889 1629 43
Fragments arranged by size:
1629 1425 889 631 405 287 275 240
196 159 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2117 3748 1537
Size: 275 305 1631 4090
Fragments arranged by size:
4090 1631 305 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4231 1353
Size: 2878 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2923 3211 4888 5262 5408 387
Size: 1064 138 1334 288 1677 374 146 1280
Fragments arranged by size:
1677 1334 1280 1064 374 288 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2441 2919 4364 4961 5748 6016 1239
Size: 393 809 478 1445 597 787 268 1524
Fragments arranged by size:
1524 1445 809 787 597 478 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2599 2729 2599
Size: 130 6171
EcoRI G'AATT_C
Cuts at: 3610 3988 3610
Size: 378 5923
EcoRV GAT'ATC
Cuts at: 2155 2155
Size: 6301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2318 2362 2318
Size: 44 6257
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 2041 2072 5448 1678
Size: 363 31 3376 2531
Fragments arranged by size:
3376 2531 363 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3063 3095 4942 4974 5016
Size: 32 135 32 1491 32 1847 32 42
Cuts at: 5016 5048 1373
Size: 32 2626
Fragments arranged by size:
2626 1847 1491 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3121 3645 3891 669
Size: 988 281 1183 524 246 3079
Fragments arranged by size:
3079 1183 988 524 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6301
HpaI GTT'AAC
Cuts at: 3891 3891
Size: 6301
KasI G'GCGC_C
Cuts at: 2068 2068
Size: 6301
KpnI G_GTAC'C
Cuts at: 2878 3626 2878
Size: 748 5553
MfeI C'AATT_G
Cuts at: 3900 3900
Size: 6301
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2182 5415 5599 1305
Size: 877 3233 184 2007
Fragments arranged by size:
3233 2007 877 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2750 3554 10
Size: 54 1425 343 918 804 2757
Fragments arranged by size:
2757 1425 918 804 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2466 4259 4618 4777 518
Size: 648 1300 1793 359 159 2042
Fragments arranged by size:
2042 1793 1300 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2894 3502 3717 4394 5542 5787 1606
Size: 1288 608 215 677 1148 245 2120
Fragments arranged by size:
2120 1288 1148 677 608 245 215
NarI GG'CG_CC
Cuts at: 2069 2069
Size: 6301
NcoI C'CATG_G
Cuts at: 513 1065 2004 3549 513
Size: 552 939 1545 3265
Fragments arranged by size:
3265 1545 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6301
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6301
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 2377 2429 2976 3659 5204 1132
Size: 97 1148 52 547 683 1545 2229
Fragments arranged by size:
2229 1545 1148 683 547 97 52
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6301
PciI A'CATG_T
Cuts at: 2373 2972 5200 2373
Size: 599 2228 3474
Fragments arranged by size:
3474 2228 599
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6301
PfoI T'CCnGG_A
Cuts at: 2544 2544
Size: 6301
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PmlI CAC'GTG
Cuts at: 3114 3114
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 5883 5915 4370
Size: 32 1481 32 4756
Fragments arranged by size:
4756 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6301
PsiI TTA'TAA
Cuts at: 3871 3871
Size: 6301
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2361 2393 2361
Size: 32 6269
PstI C_TGCA'G
Cuts at: 830 3653 830
Size: 2823 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
PvuII CAG'CTG
Cuts at: 3502 3502
Size: 6301
SalI G'TCGA_C
Cuts at: 1936 3643 1936
Size: 1707 4594
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 4549 6261 4549
Size: 1712 4589
SfcI C'TryA_G
Cuts at: 826 1045 2014 3649 4784 5465 5656 826
Size: 219 969 1635 1135 681 191 1471
Fragments arranged by size:
1635 1471 1135 969 681 219 191
SfoI GGC'GCC
Cuts at: 2070 2070
Size: 6301
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 3628 1872
Size: 1756 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4405 5306 5568 820
Size: 197 926 2462 901 262 1553
Fragments arranged by size:
2462 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2533 4092 493
Size: 2040 1559 2702
Fragments arranged by size:
2702 2040 1559
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3659 1132
Size: 2527 3774
SspI AAT'ATT
Cuts at: 5 52 2653 4225 5
Size: 47 2601 1572 2081
Fragments arranged by size:
2601 2081 1572 47
StuI AGG'CCT
Cuts at: 1517 3291 1517
Size: 1774 4527
StyI C'CwwG_G
Cuts at: 513 1065 2004 2364 3549 3740 513
Size: 552 939 360 1185 191 3074
Fragments arranged by size:
3074 1185 939 552 360 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3016 4328 4345 4498 4683 5128 325
Size: 1255 1436 1312 17 153 185 445 1498
Fragments arranged by size:
1498 1436 1312 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3010 4547 6259
Size: 274 80 33 51 1443 1033 1537 1712
Cuts at: 6259 96
Size: 138
Fragments arranged by size:
1712 1537 1443 1033 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6261 6293 6261
Size: 32 6269
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6301
XbaI T'CTAG_A
Cuts at: 2977 3637 2977
Size: 660 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3002 3002
Size: 6301
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6301
XmaI C'CCGG_G
Cuts at: 1870 3626 1870
Size: 1756 4545
XmnI GAAnn'nnTTC
Cuts at: 2856 4430 2856
Size: 1574 4727
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2983 4109 276
Size: 53 83 186 936 1449 1126 2468
Fragments arranged by size:
2468 1449 1126 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI NaeI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI