Restriction map for
pFN21AE4157
(Circular) (Six-base) MAPSORT of: pFN21AE4157.seq Check: 9362 from: 1 to: 6496
pFN21AE4157 6496 bp
With 182 enzymes: SgfI *
March 28, 2012 13:10 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2496 4306 278
Size: 53 83 186 936 960 1810 2468
Fragments arranged by size:
2468 1810 960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3839 1937
Size: 1902 4594
Acc65I G'GTAC_C
Cuts at: 3617 3817 3617
Size: 200 6296
AclI AA'CG_TT
Cuts at: 1563 2568 4623 4996 1563
Size: 1005 2055 373 3063
Fragments arranged by size:
3063 2055 1005 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2260 3743 4559 5943 6211 1632
Size: 628 1483 816 1384 268 1917
Fragments arranged by size:
1917 1483 1384 816 628 268
AfeI AGC'GCT
Cuts at: 2061 2061
Size: 6496
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6299
AflIII A'CryG_T
Cuts at: 1203 5395 6372 1203
Size: 4192 977 1327
Fragments arranged by size:
4192 1327 977
AgeI A'CCGG_T
Cuts at: 5327 5327
Size: 6496
AhdI GACnn_n'nnGTC
Cuts at: 5225 5225
Size: 6496
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3366 1184
Size: 2182 4314
AlwNI CAG_nnn'CTG
Cuts at: 2710 4239 5811 2710
Size: 1529 1572 3395
Fragments arranged by size:
3395 1572 1529
ApaLI G'TGCA_C
Cuts at: 4553 5709 4553
Size: 1156 5340
ApoI r'AATT_y
Cuts at: 1493 2510 3529 3805 4031 4183 6292 1493
Size: 1017 1019 276 226 152 2109 1697
Fragments arranged by size:
2109 1697 1019 1017 276 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5050 160
Size: 872 4018 1606
Fragments arranged by size:
4018 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
AvaI C'yCGr_G
Cuts at: 1870 1943 2808 3821 1870
Size: 73 865 1013 4545
Fragments arranged by size:
4545 1013 865 73
BamHI G'GATC_C
Cuts at: 3826 4193 3826
Size: 367 6129
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3617 3666 3817 5272 618
Size: 325 217 627 1830 49 151 1455 1842
Fragments arranged by size:
1842 1830 1455 627 325 217 151 49
BanII G_rGCy'C
Cuts at: 1391 3815 1391
Size: 2424 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5525
BbvCI CC'TCA_GC
Cuts at: 2010 2034 2010
Size: 24 6472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2871 2905 4687 4721 1903
Size: 34 934 34 1782 34 3678
Fragments arranged by size:
3678 1782 934 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2423 4388 5598 2423
Size: 1965 1210 3321
Fragments arranged by size:
3321 1965 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6496
BfrBI ATG'CAT
Cuts at: 2886 3537 2886
Size: 651 5845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5107 136
Size: 107 122 71 631 605 3435 1525
Fragments arranged by size:
3435 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3604 6491 3604
Size: 2887 3609
BlpI GC'TnA_GC
Cuts at: 2063 2762 3913 2063
Size: 699 1151 4646
Fragments arranged by size:
4646 1151 699
Bme1580I G_kGCm'C
Cuts at: 1790 2894 4557 5713 1790
Size: 1104 1663 1156 2573
Fragments arranged by size:
2573 1663 1156 1104
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6496
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4227 5185 442
Size: 416 53 83 399 24 2810 958 1753
Fragments arranged by size:
2810 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2495 2671 5156 1239
Size: 1256 176 2485 2579
Fragments arranged by size:
2579 2485 1256 176
Bpu10I CC'TnA_GC
Cuts at: 2010 2034 3031 2010
Size: 24 997 5475
Fragments arranged by size:
5475 997 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2576 4621 5486 5784 2576
Size: 2045 865 298 3288
Fragments arranged by size:
3288 2045 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5159 882
Size: 617 3660 2219
Fragments arranged by size:
3660 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3634 4287 493
Size: 711 2430 653 2702
Fragments arranged by size:
2702 2430 711 653
BsaBI GATnn'nnATC
Cuts at: 6490 6490
Size: 6496
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2493 3447 4303 4685
Size: 53 83 186 936 960 954 856 382
Cuts at: 4685 5320 275
Size: 635 1451
Fragments arranged by size:
1451 960 954 936 856 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4929 5327 5601 5748 6159 4929
Size: 398 274 147 411 5266
Fragments arranged by size:
5266 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6271
Fragments arranged by size:
6271 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2135 3159 3388 1186
Size: 907 42 1024 229 4294
Fragments arranged by size:
4294 1024 907 229 42
BseYI C'CCAG_C
Cuts at: 3339 5699 6195 3339
Size: 2360 496 3640
Fragments arranged by size:
3640 2360 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 3735 1163
Size: 573 417 1582 3924
Fragments arranged by size:
3924 1582 573 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3946 4707 5735 1540
Size: 275 186 1945 761 1028 2301
Fragments arranged by size:
2301 1945 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4557 4642 5713 4557
Size: 85 1071 5340
Fragments arranged by size:
5340 1071 85
BsmI GAATG_Cn'
Cuts at: 4007 4100 4007
Size: 93 6403
BsmBI CGTCTCn'nnnn_
Cuts at: 2484 2484
Size: 6496
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2894 3815 4557 4642 5713 1391
Size: 399 1104 921 742 85 1071 2174
Fragments arranged by size:
2174 1104 1071 921 742 399 85
BspEI T'CCGG_A
Cuts at: 6159 6159
Size: 6496
BspHI T'CATG_A
Cuts at: 4385 6115 4385
Size: 1730 4766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2343 3851 844
Size: 1499 1508 3489
Fragments arranged by size:
3489 1508 1499
BsrBI CCG'CTC
Cuts at: 1394 1823 2761 3386 4383 1394
Size: 429 938 625 997 3507
Fragments arranged by size:
3507 997 938 625 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2388 2581 3071 3726 4991 5165 65
Size: 1183 1140 193 490 655 1265 174 1396
Fragments arranged by size:
1396 1265 1183 1140 655 490 193 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5140 5327 1700
Size: 110 3330 187 2869
Fragments arranged by size:
3330 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3132 96
Size: 3036 3460
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6496
BssSI C'ACGA_G
Cuts at: 4556 5568 4556
Size: 1012 5484
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6496
BstBI TT'CG_AA
Cuts at: 5300 5300
Size: 6496
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6496
BstYI r'GATC_y
Cuts at: 1972 2124 2259 3510 3604 3826 4193 4578 4595
Size: 152 135 1251 94 222 367 385 17
Cuts at: 4595 6047 6133 6145 6491 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1251 385 367 346 222 152
135 94 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2959 3174 3228 3279 513
Size: 552 939 955 215 54 51 3730
Fragments arranged by size:
3730 955 939 552 215 54 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2801 2891 2896 487
Size: 683 56 1575 90 5 4087
Fragments arranged by size:
4087 1575 683 90 56 5
BtsI GCAGTG_nn'
Cuts at: 2023 3350 4012 4806 4826 2023
Size: 1327 662 794 20 3693
Fragments arranged by size:
3693 1327 794 662 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6496
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6461
DraI TTT'AAA
Cuts at: 3502 3646 3800 3502
Size: 144 154 6198
Fragments arranged by size:
6198 154 144
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5503 809
Size: 507 4187 1802
Fragments arranged by size:
4187 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2154 2793 3943
Size: 54 1425 50 275 18 324 639 1150
Cuts at: 3943 4832 6461 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1150 889 639 324 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3943 1537
Size: 275 2131 4090
Fragments arranged by size:
4090 2131 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2841 4426 1353
Size: 1488 1585 3423
Fragments arranged by size:
3423 1585 1488
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3737 5083 5457 5603 387
Size: 1064 138 2148 1346 374 146 1280
Fragments arranged by size:
2148 1346 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2260 2495 2671 3743 4559 5156 5943
Size: 393 628 235 176 1072 816 597 787
Cuts at: 5943 6211 1239
Size: 268 1524
Fragments arranged by size:
1524 1072 816 787 628 597 393 268
235 176
EcoO109I rG'GnC_Cy
Cuts at: 3336 3336
Size: 6496
EcoRI G'AATT_C
Cuts at: 2510 3805 4183 2510
Size: 1295 378 4823
Fragments arranged by size:
4823 1295 378
EcoRV GAT'ATC
Cuts at: 2238 2238
Size: 6496
FspI TGC'GCA
Cuts at: 5002 5002
Size: 6496
HaeII r_GCGC'y
Cuts at: 1678 2063 5643 1678
Size: 385 3580 2531
Fragments arranged by size:
3580 2531 385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2642 2674 2721
Size: 32 135 32 620 32 418 32 47
Cuts at: 2721 2753 5137 5169 5211 5243 1373
Size: 32 2384 32 42 32 2626
Fragments arranged by size:
2626 2384 620 418 135 47 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3840 4086 669
Size: 988 281 1902 246 3079
Fragments arranged by size:
3079 1902 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2534 3302 3740 748
Size: 1786 768 438 3504
Fragments arranged by size:
3504 1786 768 438
HpaI GTT'AAC
Cuts at: 4086 4086
Size: 6496
KpnI G_GTAC'C
Cuts at: 3621 3821 3621
Size: 200 6296
MfeI C'AATT_G
Cuts at: 4095 4095
Size: 6496
MluI A'CGCG_T
Cuts at: 6372 6372
Size: 6496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2661 3717 5610 5794 1305
Size: 1356 1056 1893 184 2007
Fragments arranged by size:
2007 1893 1356 1056 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2795 10
Size: 54 1425 343 963 3711
Fragments arranged by size:
3711 1425 963 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4454 4813 4972 518
Size: 648 3288 359 159 2042
Fragments arranged by size:
3288 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2330 3912 4589 5737 5982 1606
Size: 724 1582 677 1148 245 2120
Fragments arranged by size:
2120 1582 1148 724 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2959 3174 3228 3279 513
Size: 552 939 955 215 54 51 3730
Fragments arranged by size:
3730 955 939 552 215 54 51
NdeI CA'TA_TG
Cuts at: 387 3356 387
Size: 2969 3527
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6496
NotI GC'GGCC_GC
Cuts at: 3943 3943
Size: 6496
NsiI A_TGCA'T
Cuts at: 2888 3539 2888
Size: 651 5845
NspI r_CATG'y
Cuts at: 1132 1229 2890 2947 3854 5399 1132
Size: 97 1661 57 907 1545 2229
Fragments arranged by size:
2229 1661 1545 907 97 57
PasI CC'CwG_GG
Cuts at: 1348 3451 1348
Size: 2103 4393
PciI A'CATG_T
Cuts at: 5395 5395
Size: 6496
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6496
PfoI T'CCnGG_A
Cuts at: 2262 2262
Size: 6496
PmeI GTTT'AAAC
Cuts at: 3800 3800
Size: 6496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4565 4597 6078 6110 4565
Size: 32 1481 32 4951
Fragments arranged by size:
4951 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3336 3336
Size: 6496
PshAI GACnn'nnGTC
Cuts at: 1613 3230 1613
Size: 1617 4879
PsiI TTA'TAA
Cuts at: 2217 4066 2217
Size: 1849 4647
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2709 2741 2709
Size: 32 6464
PstI C_TGCA'G
Cuts at: 830 3756 3848 830
Size: 2926 92 3478
Fragments arranged by size:
3478 2926 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6496
PvuII CAG'CTG
Cuts at: 2330 2330
Size: 6496
SalI G'TCGA_C
Cuts at: 1936 3838 1936
Size: 1902 4594
SbfI CC_TGCA'GG
Cuts at: 3848 3848
Size: 6496
ScaI AGT'ACT
Cuts at: 4744 6456 4744
Size: 1712 4784
SexAI A'CCwGG_T
Cuts at: 2103 2103
Size: 6496
SfcI C'TryA_G
Cuts at: 826 1045 3315 3576 3752 3844 4979 5660 5851
Size: 219 2270 261 176 92 1135 681 191
Cuts at: 5851 826
Size: 1471
Fragments arranged by size:
2270 1471 1135 681 261 219 191 176
92
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6496
SmaI CCC'GGG
Cuts at: 1872 2810 3823 1872
Size: 938 1013 4545
Fragments arranged by size:
4545 1013 938
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2555 4600 5501 5763 820
Size: 197 926 612 2045 901 262 1553
Fragments arranged by size:
2045 1553 926 901 612 262 197
SnaBI TAC'GTA
Cuts at: 493 4287 493
Size: 3794 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6496
SphI G_CATG'C
Cuts at: 1132 2947 3854 1132
Size: 1815 907 3774
Fragments arranged by size:
3774 1815 907
SspI AAT'ATT
Cuts at: 5 52 4420 5
Size: 47 4368 2081
Fragments arranged by size:
4368 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6496
StyI C'CwwG_G
Cuts at: 513 1065 2004 2959 3174 3228 3279 3935 513
Size: 552 939 955 215 54 51 656 3074
Fragments arranged by size:
3074 955 939 656 552 215 54 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3272 4523 4540 4693 4878 5323 325
Size: 1255 1692 1251 17 153 185 445 1498
Fragments arranged by size:
1692 1498 1255 1251 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3132 4742 6454
Size: 274 80 33 51 1443 1155 1610 1712
Cuts at: 6454 96
Size: 138
Fragments arranged by size:
1712 1610 1443 1155 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6456 6488 6456
Size: 32 6464
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6496
XbaI T'CTAG_A
Cuts at: 3832 3832
Size: 6496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2470 2470
Size: 6496
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6496
XmaI C'CCGG_G
Cuts at: 1870 2808 3821 1870
Size: 938 1013 4545
Fragments arranged by size:
4545 1013 938
XmnI GAAnn'nnTTC
Cuts at: 4625 4625
Size: 6496
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2494 4304 276
Size: 53 83 186 936 960 1810 2468
Fragments arranged by size:
2468 1810 960 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI