Restriction map for
pFN21AE4154
(Circular) (Six-base) MAPSORT of: pFN21AE4154.seq Check: 1814 from: 1 to: 6472
pFN21AE4154 6472 bp
With 182 enzymes: SgfI *
March 28, 2012 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2061 2848 2061
Size: 787 5685
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2103 2204 2912 4282
Size: 53 83 186 936 567 101 708 1370
Cuts at: 4282 278
Size: 2468
Fragments arranged by size:
2468 1370 936 708 567 186 101 83
53
AccI GT'mk_AC
Cuts at: 1937 3815 1937
Size: 1878 4594
Acc65I G'GTAC_C
Cuts at: 3793 3793
Size: 6472
AclI AA'CG_TT
Cuts at: 1563 4599 4972 1563
Size: 3036 373 3063
Fragments arranged by size:
3063 3036 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3173 4535 5919 6187 1632
Size: 1541 1362 1384 268 1917
Fragments arranged by size:
1917 1541 1384 1362 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6275
AflIII A'CryG_T
Cuts at: 1203 5371 6348 1203
Size: 4168 977 1327
Fragments arranged by size:
4168 1327 977
AgeI A'CCGG_T
Cuts at: 5303 5303
Size: 6472
AhdI GACnn_n'nnGTC
Cuts at: 5201 5201
Size: 6472
AleI CACnn'nnGTG
Cuts at: 1166 3101 1166
Size: 1935 4537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6472
AlwNI CAG_nnn'CTG
Cuts at: 4215 5787 4215
Size: 1572 4900
ApaI G_GGCC'C
Cuts at: 3056 3214 3540 3603 3056
Size: 158 326 63 5925
Fragments arranged by size:
5925 326 158 63
ApaLI G'TGCA_C
Cuts at: 3505 4529 5685 3505
Size: 1024 1156 4292
Fragments arranged by size:
4292 1156 1024
ApoI r'AATT_y
Cuts at: 1493 3781 4007 4159 6268 1493
Size: 2288 226 152 2109 1697
Fragments arranged by size:
2288 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5026 160
Size: 872 3994 1606
Fragments arranged by size:
3994 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
AvaI C'yCGr_G
Cuts at: 1870 1943 3797 1870
Size: 73 1854 4545
Fragments arranged by size:
4545 1854 73
BamHI G'GATC_C
Cuts at: 3802 4169 3802
Size: 367 6105
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2049 2444 2890 3492 3793
Size: 325 217 627 262 395 446 602 301
Cuts at: 3793 5248 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 602 446 395 325 301
262 217
BanII G_rGCy'C
Cuts at: 1391 2067 2613 3003 3056 3214 3540 3572 3603
Size: 676 546 390 53 158 326 32 31
Cuts at: 3603 3791 1391
Size: 188 4072
Fragments arranged by size:
4072 676 546 390 326 188 158 53
32 31
BbeI G_GCGC'C
Cuts at: 2448 3496 2448
Size: 1048 5424
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2119 2204 3442 3717 928
Size: 971 220 85 1238 275 3683
Fragments arranged by size:
3683 1238 971 275 220 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2987 3021 4663 4697 1903
Size: 34 1050 34 1642 34 3678
Fragments arranged by size:
3678 1642 1050 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4364 5574 4364
Size: 1210 5262
BclI T'GATC_A
Cuts at: 1548 3243 1548
Size: 1695 4777
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2489 2653 5083
Size: 107 122 71 631 605 817 164 2430
Cuts at: 5083 136
Size: 1525
Fragments arranged by size:
2430 1525 817 631 605 164 122 107
71
BglII A'GATC_T
Cuts at: 3351 6467 3351
Size: 3116 3356
BlpI GC'TnA_GC
Cuts at: 3889 3889
Size: 6472
Bme1580I G_kGCm'C
Cuts at: 1790 3056 3214 3509 3540 3603 4533 5689 1790
Size: 1266 158 295 31 63 930 1156 2573
Fragments arranged by size:
2573 1266 1156 930 295 158 63 31
BmgBI CAC'GTC
Cuts at: 1318 2665 3163 1318
Size: 1347 498 4627
Fragments arranged by size:
4627 1347 498
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4203 5161 442
Size: 416 53 83 399 24 2786 958 1753
Fragments arranged by size:
2786 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6472
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3730 3730
Size: 6472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2679 3472 3558 3629 5132 1239
Size: 1440 793 86 71 1503 2579
Fragments arranged by size:
2579 1503 1440 793 86 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3287 4597 5462 5760 3287
Size: 1310 865 298 3999
Fragments arranged by size:
3999 1310 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2828 3029 5135 882
Size: 617 1329 201 2106 2219
Fragments arranged by size:
2219 2106 1329 617 201
BsaAI yAC'GTr
Cuts at: 493 1204 4263 493
Size: 711 3059 2702
Fragments arranged by size:
3059 2702 711
BsaBI GATnn'nnATC
Cuts at: 6466 6466
Size: 6472
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 2100 2201 2412
Size: 53 83 186 936 555 12 101 211
Cuts at: 2412 2445 2909 3081 3493 4279 4661 5296 275
Size: 33 464 172 412 786 382 635 1451
Fragments arranged by size:
1451 936 786 635 555 464 412 382
211 186 172 101 83 53 33 12
BsaWI w'CCGG_w
Cuts at: 2546 4905 5303 5577 5724 6135 2546
Size: 2359 398 274 147 411 2883
Fragments arranged by size:
2883 2359 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6247
Fragments arranged by size:
6247 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2512 3000 3032 3048 3656 1186
Size: 1326 488 32 16 608 4002
Fragments arranged by size:
4002 1326 608 488 32 16
BseYI C'CCAG_C
Cuts at: 2152 3166 3646 5675 6171 2152
Size: 1014 480 2029 496 2453
Fragments arranged by size:
2453 2029 1014 496 480
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2213 2772 3460 1163
Size: 573 477 559 688 4175
Fragments arranged by size:
4175 688 573 559 477
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2484 3725 3922 4683 5711 1540
Size: 275 186 483 1241 197 761 1028 2301
Fragments arranged by size:
2301 1241 1028 761 483 275 197 186
BsiHKAI G_wGCw'C
Cuts at: 2067 3003 3384 3509 4533 4618 5689 2067
Size: 936 381 125 1024 85 1071 2850
Fragments arranged by size:
2850 1071 1024 936 381 125 85
BsmI GAATG_Cn'
Cuts at: 3983 4076 3983
Size: 93 6379
BsmBI CGTCTCn'nnnn_
Cuts at: 2655 2715 2655
Size: 60 6412
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2613 3003 3056 3214 3384 3509
Size: 399 277 546 390 53 158 170 125
Cuts at: 3509 3540 3572 3603 3791 4533 4618 5689 1391
Size: 31 32 31 188 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 546 399 390 277 188
170 158 125 85 53 32 31 31
BspEI T'CCGG_A
Cuts at: 2546 6135 2546
Size: 3589 2883
BspHI T'CATG_A
Cuts at: 3126 4361 6091 3126
Size: 1235 1730 3507
Fragments arranged by size:
3507 1730 1235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2061 2689 2848 3120 3207 3827 844
Size: 1217 628 159 272 87 620 3489
Fragments arranged by size:
3489 1217 628 620 272 159 87
BsrBI CCG'CTC
Cuts at: 1394 1823 3334 4359 1394
Size: 429 1511 1025 3507
Fragments arranged by size:
3507 1511 1025 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4967 5141 65
Size: 1183 3719 174 1396
Fragments arranged by size:
3719 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2046 5116 5303 1700
Size: 110 236 3070 187 2869
Fragments arranged by size:
3070 2869 236 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6472
BssHII G'CGCG_C
Cuts at: 1925 3115 1925
Size: 1190 5282
BssSI C'ACGA_G
Cuts at: 3627 4532 5544 3627
Size: 905 1012 4555
Fragments arranged by size:
4555 1012 905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2056 3438 794
Size: 1262 1382 3828
Fragments arranged by size:
3828 1382 1262
BstBI TT'CG_AA
Cuts at: 5276 5276
Size: 6472
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6472
BstXI CCAn_nnnn'nTGG
Cuts at: 3049 3103 3049
Size: 54 6418
BstYI r'GATC_y
Cuts at: 1972 3351 3802 4169 4554 4571 6023 6109 6121
Size: 1379 451 367 385 17 1452 86 12
Cuts at: 6121 6467 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1379 451 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3618 3675 3618
Size: 57 6415
BtgI C'CryG_G
Cuts at: 513 1065 2214 2510 2802 3099 3496 3523 3547
Size: 552 1149 296 292 297 397 27 24
Cuts at: 3547 3719 513
Size: 172 3266
Fragments arranged by size:
3266 1149 552 397 297 296 292 172
27 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2545 487
Size: 683 56 1319 4414
Fragments arranged by size:
4414 1319 683 56
BtsI GCAGTG_nn'
Cuts at: 3988 4782 4802 3988
Size: 794 20 5658
Fragments arranged by size:
5658 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6472
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6437
DraI TTT'AAA
Cuts at: 3776 3776
Size: 6472
DraIII CAC_nnn'GTG
Cuts at: 3502 3502
Size: 6472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2954 5479 809
Size: 507 1638 2525 1802
Fragments arranged by size:
2525 1802 1638 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2481 2490 2556
Size: 54 1425 50 275 18 651 9 66
Cuts at: 2556 2918 3105 3282 3520 3531 3722 3919 4808
Size: 362 187 177 238 11 191 197 889
Cuts at: 4808 6437 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 651 362 275 238 197
191 187 177 66 54 50 43 18
11 9
EagI C'GGCC_G
Cuts at: 1537 1812 2481 3722 3919 1537
Size: 275 669 1241 197 4090
Fragments arranged by size:
4090 1241 669 275 197
EarI CTCTTCn'nnn_
Cuts at: 1353 2688 4402 1353
Size: 1335 1714 3423
Fragments arranged by size:
3423 1714 1335
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3448 3570 3653 3707 5059 5433
Size: 1064 138 1859 122 83 54 1352 374
Cuts at: 5433 5579 387
Size: 146 1280
Fragments arranged by size:
1859 1352 1280 1064 374 146 138 122
83 54
EcoICRI GAG'CTC
Cuts at: 2065 3001 2065
Size: 936 5536
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2679 3173 3472 3558 3629 4535 5132
Size: 393 1047 494 299 86 71 906 597
Cuts at: 5132 5919 6187 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1047 906 787 597 494 393 299
268 86 71
EcoNI CCTnn'n_nnAGG
Cuts at: 3089 3089
Size: 6472
EcoO109I rG'GnC_Cy
Cuts at: 3053 3210 3537 3600 3613 3622 3664 3743 3053
Size: 157 327 63 13 9 42 79 5782
Fragments arranged by size:
5782 327 157 79 63 42 13 9
EcoRI G'AATT_C
Cuts at: 3781 4159 3781
Size: 378 6094
FspI TGC'GCA
Cuts at: 2684 4978 2684
Size: 2294 4178
HaeII r_GCGC'y
Cuts at: 1678 2294 2390 2448 3125 3394 3496 3584 5619
Size: 616 96 58 677 269 102 88 2035
Cuts at: 5619 1678
Size: 2531
Fragments arranged by size:
2531 2035 677 616 269 102 96 88
58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2306 2338 3164 3196 5113
Size: 32 135 32 734 32 826 32 1917
Cuts at: 5113 5145 5187 5219 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1917 826 734 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3816 4062 669
Size: 988 281 1878 246 3079
Fragments arranged by size:
3079 1878 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6472
HpaI GTT'AAC
Cuts at: 4062 4062
Size: 6472
KasI G'GCGC_C
Cuts at: 2444 3492 2444
Size: 1048 5424
KpnI G_GTAC'C
Cuts at: 3797 3797
Size: 6472
MfeI C'AATT_G
Cuts at: 4071 4071
Size: 6472
MluI A'CGCG_T
Cuts at: 6348 6348
Size: 6472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2184 2388 3000 5586 5770 1305
Size: 879 204 612 2586 184 2007
Fragments arranged by size:
2586 2007 879 612 204 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3107 10
Size: 54 1425 343 1275 3375
Fragments arranged by size:
3375 1425 1275 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 3101 4430 4789 4948 518
Size: 648 1149 786 1329 359 159 2042
Fragments arranged by size:
2042 1329 1149 786 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2568 3101 3525 3721 3888 4565 5713 5958
Size: 962 533 424 196 167 677 1148 245
Cuts at: 5958 1606
Size: 2120
Fragments arranged by size:
2120 1148 962 677 533 424 245 196
167
NarI GG'CG_CC
Cuts at: 2445 3493 2445
Size: 1048 5424
NcoI C'CATG_G
Cuts at: 513 1065 3547 513
Size: 552 2482 3438
Fragments arranged by size:
3438 2482 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6472
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6472
NotI GC'GGCC_GC
Cuts at: 3722 3919 3722
Size: 197 6275
NspI r_CATG'y
Cuts at: 1132 1229 3316 3830 5375 1132
Size: 97 2087 514 1545 2229
Fragments arranged by size:
2229 2087 1545 514 97
PasI CC'CwG_GG
Cuts at: 1348 2245 1348
Size: 897 5575
PciI A'CATG_T
Cuts at: 5371 5371
Size: 6472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3400 1108
Size: 2292 4180
PmeI GTTT'AAAC
Cuts at: 3776 3776
Size: 6472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2753 2785 3569 3601 4541 4573 6054 6086 2753
Size: 32 784 32 940 32 1481 32 3139
Fragments arranged by size:
3139 1481 940 784 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3613 3622 3613
Size: 9 6463
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6472
PsiI TTA'TAA
Cuts at: 4042 4042
Size: 6472
PspOMI G'GGCC_C
Cuts at: 3052 3210 3536 3599 3052
Size: 158 326 63 5925
Fragments arranged by size:
5925 326 158 63
PstI C_TGCA'G
Cuts at: 830 2343 3275 3824 830
Size: 1513 932 549 3478
Fragments arranged by size:
3478 1513 932 549
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6472
PvuII CAG'CTG
Cuts at: 2568 2568
Size: 6472
SacI G_AGCT'C
Cuts at: 2067 3003 2067
Size: 936 5536
SacII CC_GC'GG
Cuts at: 3102 3526 3722 3102
Size: 424 196 5852
Fragments arranged by size:
5852 424 196
SalI G'TCGA_C
Cuts at: 1936 3814 1936
Size: 1878 4594
SapI GCTCTTCn'nnn_
Cuts at: 2688 2688
Size: 6472
SbfI CC_TGCA'GG
Cuts at: 3824 3824
Size: 6472
ScaI AGT'ACT
Cuts at: 4720 6432 4720
Size: 1712 4760
SfcI C'TryA_G
Cuts at: 826 1045 2339 3271 3820 4955 5636 5827 826
Size: 219 1294 932 549 1135 681 191 1471
Fragments arranged by size:
1471 1294 1135 932 681 549 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2489 2489
Size: 6472
SfoI GGC'GCC
Cuts at: 2446 3494 2446
Size: 1048 5424
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6472
SmaI CCC'GGG
Cuts at: 1872 3799 1872
Size: 1927 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3302 4576 5477 5739 820
Size: 197 926 1359 1274 901 262 1553
Fragments arranged by size:
1553 1359 1274 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4263 493
Size: 3770 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6472
SphI G_CATG'C
Cuts at: 1132 3830 1132
Size: 2698 3774
SspI AAT'ATT
Cuts at: 5 52 4396 5
Size: 47 4344 2081
Fragments arranged by size:
4344 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6472
StyI C'CwwG_G
Cuts at: 513 1065 2613 2742 3547 3911 513
Size: 552 1548 129 805 364 3074
Fragments arranged by size:
3074 1548 805 552 364 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4499 4516 4669 4854 5299 325
Size: 1255 2919 17 153 185 445 1498
Fragments arranged by size:
2919 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2274 2755 4718
Size: 274 80 33 51 1443 297 481 1963
Cuts at: 4718 6430 96
Size: 1712 138
Fragments arranged by size:
1963 1712 1443 481 297 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3206 3238 6432 6464 3206
Size: 32 3194 32 3214
Fragments arranged by size:
3214 3194 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2234 1316
Size: 918 5554
XbaI T'CTAG_A
Cuts at: 3808 3808
Size: 6472
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6472
XmaI C'CCGG_G
Cuts at: 1870 3797 1870
Size: 1927 4545
XmnI GAAnn'nnTTC
Cuts at: 2765 3233 3254 3365 4601 2765
Size: 468 21 111 1236 4636
Fragments arranged by size:
4636 1236 468 111 21
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2101 2202 2910 4280
Size: 53 83 186 936 567 101 708 1370
Cuts at: 4280 276
Size: 2468
Fragments arranged by size:
2468 1370 936 708 567 186 101 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SanDI SexAI SrfI SwaI XcmI