Restriction map for
pFN21AE4148
(Circular) (Six-base) MAPSORT of: pFN21AE4148.seq Check: 5088 from: 1 to: 6454
pFN21AE4148 6454 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4264 278
Size: 53 83 186 936 2728 2468
Fragments arranged by size:
2728 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3797 1937
Size: 1860 4594
Acc65I G'GTAC_C
Cuts at: 3775 3775
Size: 6454
AclI AA'CG_TT
Cuts at: 1563 3086 4581 4954 1563
Size: 1523 1495 373 3063
Fragments arranged by size:
3063 1523 1495 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2934 2979 4517 5901 6169 1632
Size: 1302 45 1538 1384 268 1917
Fragments arranged by size:
1917 1538 1384 1302 268 45
AflII C'TTAA_G
Cuts at: 820 1017 2083 2141 820
Size: 197 1066 58 5133
Fragments arranged by size:
5133 1066 197 58
AflIII A'CryG_T
Cuts at: 1203 5353 6330 1203
Size: 4150 977 1327
Fragments arranged by size:
4150 1327 977
AgeI A'CCGG_T
Cuts at: 5285 5285
Size: 6454
AhdI GACnn_n'nnGTC
Cuts at: 5183 5183
Size: 6454
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3714 1184
Size: 2530 3924
AlwNI CAG_nnn'CTG
Cuts at: 2051 3374 4197 5769 2051
Size: 1323 823 1572 2736
Fragments arranged by size:
2736 1572 1323 823
ApaLI G'TGCA_C
Cuts at: 4511 5667 4511
Size: 1156 5298
ApoI r'AATT_y
Cuts at: 1493 2692 2816 3071 3637 3763 3989 4141 6250
Size: 1199 124 255 566 126 226 152 2109
Cuts at: 6250 1493
Size: 1697
Fragments arranged by size:
2109 1697 1199 566 255 226 152 126
124
AseI AT'TA_AT
Cuts at: 160 1032 2180 5008 160
Size: 872 1148 2828 1606
Fragments arranged by size:
2828 1606 1148 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6454
AvaI C'yCGr_G
Cuts at: 1870 1943 3150 3779 1870
Size: 73 1207 629 4545
Fragments arranged by size:
4545 1207 629 73
BamHI G'GATC_C
Cuts at: 3784 4151 3784
Size: 367 6087
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3775 5230 618
Size: 325 217 627 1988 1455 1842
Fragments arranged by size:
1988 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3138 3773 1391
Size: 1747 635 4072
Fragments arranged by size:
4072 1747 635
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2832 928
Size: 971 933 4550
Fragments arranged by size:
4550 971 933
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4645 4679 1903
Size: 34 2708 34 3678
Fragments arranged by size:
3678 2708 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4346 5556 4346
Size: 1210 5244
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6454
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5065 136
Size: 107 122 71 631 605 3393 1525
Fragments arranged by size:
3393 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6449 6449
Size: 6454
BlpI GC'TnA_GC
Cuts at: 3871 3871
Size: 6454
Bme1580I G_kGCm'C
Cuts at: 1790 2455 4515 5671 1790
Size: 665 2060 1156 2573
Fragments arranged by size:
2573 2060 1156 665
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6454
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4185 5143 442
Size: 416 53 83 399 24 2768 958 1753
Fragments arranged by size:
2768 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6454
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 2987
Size: 6454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5114 1239
Size: 3875 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4579 5444 5742 4579
Size: 865 298 5291
Fragments arranged by size:
5291 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3221 5117 882
Size: 617 1722 1896 2219
Fragments arranged by size:
2219 1896 1722 617
BsaAI yAC'GTr
Cuts at: 493 1204 4245 493
Size: 711 3041 2702
Fragments arranged by size:
3041 2702 711
BsaBI GATnn'nnATC
Cuts at: 6448 6448
Size: 6454
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4261 4643 5278 275
Size: 53 83 186 936 2728 382 635 1451
Fragments arranged by size:
2728 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4887 5285 5559 5706 6117 4887
Size: 398 274 147 411 5224
Fragments arranged by size:
5224 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6229
Fragments arranged by size:
6229 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6454
BseYI C'CCAG_C
Cuts at: 5657 6153 5657
Size: 496 5958
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2247 3720 1163
Size: 573 511 1473 3897
Fragments arranged by size:
3897 1473 573 511
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3904 4665 5693 1540
Size: 275 186 1903 761 1028 2301
Fragments arranged by size:
2301 1903 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4515 4600 5671 4515
Size: 85 1071 5298
Fragments arranged by size:
5298 1071 85
BsmI GAATG_Cn'
Cuts at: 2598 2667 3676 3965 4058 2598
Size: 69 1009 289 93 4994
Fragments arranged by size:
4994 1009 289 93 69
BsmBI CGTCTCn'nnnn_
Cuts at: 2931 2931
Size: 6454
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2455 3138 3773 4515 4600 5671 1391
Size: 399 665 683 635 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 683 665 635 399 85
BspEI T'CCGG_A
Cuts at: 6117 6117
Size: 6454
BspHI T'CATG_A
Cuts at: 4343 6073 4343
Size: 1730 4724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3809 844
Size: 2965 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4341 1394
Size: 429 2518 3507
Fragments arranged by size:
3507 2518 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3681 4949 5123 65
Size: 1183 2433 1268 174 1396
Fragments arranged by size:
2433 1396 1268 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5098 5285 1700
Size: 110 3288 187 2869
Fragments arranged by size:
3288 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6454
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6454
BssSI C'ACGA_G
Cuts at: 4514 5526 4514
Size: 1012 5442
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2051 2270 794
Size: 1257 219 4978
Fragments arranged by size:
4978 1257 219
BstBI TT'CG_AA
Cuts at: 2713 5258 2713
Size: 2545 3909
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6454
BstYI r'GATC_y
Cuts at: 1972 3784 4151 4536 4553 6005 6091 6103 6449
Size: 1812 367 385 17 1452 86 12 346
Cuts at: 6449 1972
Size: 1977
Fragments arranged by size:
1977 1812 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4963
Fragments arranged by size:
4963 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3219 487
Size: 683 56 1993 3722
Fragments arranged by size:
3722 1993 683 56
BtsI GCAGTG_nn'
Cuts at: 2038 2436 3970 4764 4784 2038
Size: 398 1534 794 20 3708
Fragments arranged by size:
3708 1534 794 398 20
ClaI AT'CG_AT
Cuts at: 1554 2640 1554
Size: 1086 5368
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6419
DraI TTT'AAA
Cuts at: 2200 3758 2200
Size: 1558 4896
DraIII CAC_nnn'GTG
Cuts at: 2936 2936
Size: 6454
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5461 809
Size: 507 4145 1802
Fragments arranged by size:
4145 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3901 4790 6419
Size: 54 1425 50 275 18 2071 889 1629
Cuts at: 6419 8
Size: 43
Fragments arranged by size:
2071 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3901 1537
Size: 275 2089 4090
Fragments arranged by size:
4090 2089 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3219 3614 4384 1353
Size: 1866 395 770 3423
Fragments arranged by size:
3423 1866 770 395
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5041 5415 5561 387
Size: 1064 138 3452 374 146 1280
Fragments arranged by size:
3452 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2934 2979 4517 5114 5901 6169 1239
Size: 393 1302 45 1538 597 787 268 1524
Fragments arranged by size:
1538 1524 1302 787 597 393 268 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2916 2916
Size: 6454
EcoO109I rG'GnC_Cy
Cuts at: 2909 2909
Size: 6454
EcoRI G'AATT_C
Cuts at: 3763 4141 3763
Size: 378 6076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 6454
FspI TGC'GCA
Cuts at: 4960 4960
Size: 6454
HaeII r_GCGC'y
Cuts at: 1678 5601 1678
Size: 3923 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2672 2704 5095
Size: 32 135 32 509 32 559 32 2391
Cuts at: 5095 5127 5169 5201 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2391 559 509 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2469 3798 4044 669
Size: 988 281 531 1329 246 3079
Fragments arranged by size:
3079 1329 988 531 281 246
HindIII A'AGCT_T
Cuts at: 748 2113 2305 2840 748
Size: 1365 192 535 4362
Fragments arranged by size:
4362 1365 535 192
HpaI GTT'AAC
Cuts at: 4044 4044
Size: 6454
KpnI G_GTAC'C
Cuts at: 3779 3779
Size: 6454
MfeI C'AATT_G
Cuts at: 4053 4053
Size: 6454
MluI A'CGCG_T
Cuts at: 6330 6330
Size: 6454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5568 5752 1305
Size: 4263 184 2007
Fragments arranged by size:
4263 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4632
Fragments arranged by size:
4632 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3696 4412 4771 4930 518
Size: 648 2530 716 359 159 2042
Fragments arranged by size:
2530 2042 716 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2957 3870 4547 5695 5940 1606
Size: 1351 913 677 1148 245 2120
Fragments arranged by size:
2120 1351 1148 913 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4963
Fragments arranged by size:
4963 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6454
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6454
NotI GC'GGCC_GC
Cuts at: 3901 3901
Size: 6454
NspI r_CATG'y
Cuts at: 1132 1229 3812 5357 1132
Size: 97 2583 1545 2229
Fragments arranged by size:
2583 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6454
PciI A'CATG_T
Cuts at: 5353 5353
Size: 6454
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2660 2936 3034 1108
Size: 1552 276 98 4528
Fragments arranged by size:
4528 1552 276 98
PfoI T'CCnGG_A
Cuts at: 3367 3367
Size: 6454
PmeI GTTT'AAAC
Cuts at: 3758 3758
Size: 6454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4523 4555 6036 6068 4523
Size: 32 1481 32 4909
Fragments arranged by size:
4909 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2909 2909
Size: 6454
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6454
PsiI TTA'TAA
Cuts at: 3042 4024 3042
Size: 982 5472
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2121 2153 3245 3277 2121
Size: 32 1092 32 5298
Fragments arranged by size:
5298 1092 32 32
PstI C_TGCA'G
Cuts at: 830 3337 3806 830
Size: 2507 469 3478
Fragments arranged by size:
3478 2507 469
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6454
PvuII CAG'CTG
Cuts at: 2957 2957
Size: 6454
SalI G'TCGA_C
Cuts at: 1936 3796 1936
Size: 1860 4594
SbfI CC_TGCA'GG
Cuts at: 3806 3806
Size: 6454
ScaI AGT'ACT
Cuts at: 4702 6414 4702
Size: 1712 4742
SfcI C'TryA_G
Cuts at: 826 1045 2444 3333 3432 3802 4937 5618 5809
Size: 219 1399 889 99 370 1135 681 191
Cuts at: 5809 826
Size: 1471
Fragments arranged by size:
1471 1399 1135 889 681 370 219 191
99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6454
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6454
SmaI CCC'GGG
Cuts at: 1872 3781 1872
Size: 1909 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2083 2141 3150 4558 5459 5721
Size: 197 926 140 58 1009 1408 901 262
Cuts at: 5721 820
Size: 1553
Fragments arranged by size:
1553 1408 1009 926 901 262 197 140
58
SnaBI TAC'GTA
Cuts at: 493 4245 493
Size: 3752 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6454
SphI G_CATG'C
Cuts at: 1132 3812 1132
Size: 2680 3774
SspI AAT'ATT
Cuts at: 5 52 2041 3455 4378 5
Size: 47 1989 1414 923 2081
Fragments arranged by size:
2081 1989 1414 923 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6454
StyI C'CwwG_G
Cuts at: 513 1065 2004 3893 513
Size: 552 939 1889 3074
Fragments arranged by size:
3074 1889 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4481 4498 4651 4836 5281 325
Size: 1255 2901 17 153 185 445 1498
Fragments arranged by size:
2901 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3355 4700 6412
Size: 274 80 33 51 1443 1378 1345 1712
Cuts at: 6412 96
Size: 138
Fragments arranged by size:
1712 1443 1378 1345 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3558 3590 6414 6446 3558
Size: 32 2824 32 3566
Fragments arranged by size:
3566 2824 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6454
XbaI T'CTAG_A
Cuts at: 2671 3790 2671
Size: 1119 5335
XhoI C'TCGA_G
Cuts at: 1943 3150 1943
Size: 1207 5247
XmaI C'CCGG_G
Cuts at: 1870 3779 1870
Size: 1909 4545
XmnI GAAnn'nnTTC
Cuts at: 2719 4583 2719
Size: 1864 4590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4262 276
Size: 53 83 186 936 2728 2468
Fragments arranged by size:
2728 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI