Restriction map for
pFN21AE4145
(Circular) (Six-base) MAPSORT of: pFN21AE4145.seq Check: 3552 from: 1 to: 6439
pFN21AE4145 6439 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2046 2655 2846 2046
Size: 609 191 5639
Fragments arranged by size:
5639 609 191
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2109 4249 278
Size: 53 83 186 936 573 2140 2468
Fragments arranged by size:
2468 2140 936 573 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3453 3782 1937
Size: 1516 329 4594
Fragments arranged by size:
4594 1516 329
Acc65I G'GTAC_C
Cuts at: 3760 3760
Size: 6439
AclI AA'CG_TT
Cuts at: 1563 4566 4939 1563
Size: 3003 373 3063
Fragments arranged by size:
3063 3003 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3080 3116 4502 5886 6154 1632
Size: 1448 36 1386 1384 268 1917
Fragments arranged by size:
1917 1448 1386 1384 268 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6242
AflIII A'CryG_T
Cuts at: 1203 3456 5338 6315 1203
Size: 2253 1882 977 1327
Fragments arranged by size:
2253 1882 1327 977
AgeI A'CCGG_T
Cuts at: 5270 5270
Size: 6439
AhdI GACnn_n'nnGTC
Cuts at: 3028 5168 3028
Size: 2140 4299
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2412 1184
Size: 1228 5211
AlwNI CAG_nnn'CTG
Cuts at: 2080 2392 2694 2707 2803 2839 3297 4182 5754
Size: 312 302 13 96 36 458 885 1572
Cuts at: 5754 2080
Size: 2765
Fragments arranged by size:
2765 1572 885 458 312 302 96 36
13
ApaLI G'TGCA_C
Cuts at: 2669 4496 5652 2669
Size: 1827 1156 3456
Fragments arranged by size:
3456 1827 1156
ApoI r'AATT_y
Cuts at: 1493 3748 3974 4126 6235 1493
Size: 2255 226 152 2109 1697
Fragments arranged by size:
2255 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4993 160
Size: 872 3961 1606
Fragments arranged by size:
3961 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6439
AvaI C'yCGr_G
Cuts at: 1870 1943 2529 2594 2634 2651 3006 3467 3764
Size: 73 586 65 40 17 355 461 297
Cuts at: 3764 1870
Size: 4545
Fragments arranged by size:
4545 586 461 355 297 73 65 40
17
BamHI G'GATC_C
Cuts at: 3769 4136 3769
Size: 367 6072
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2779 3085 3155 3529 3644
Size: 325 217 627 992 306 70 374 115
Cuts at: 3644 3760 5215 618
Size: 116 1455 1842
Fragments arranged by size:
1842 1455 992 627 374 325 306 217
116 115 70
BanII G_rGCy'C
Cuts at: 1391 2247 2724 2868 3290 3423 3758 1391
Size: 856 477 144 422 133 335 4072
Fragments arranged by size:
4072 856 477 422 335 144 133
BbeI G_GCGC'C
Cuts at: 3089 3648 3089
Size: 559 5880
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3679 928
Size: 971 1780 3688
Fragments arranged by size:
3688 1780 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4630 4664 1903
Size: 34 2693 34 3678
Fragments arranged by size:
3678 2693 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4331 5541 4331
Size: 1210 5229
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6439
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3122 5050 136
Size: 107 122 71 631 605 1450 1928 1525
Fragments arranged by size:
1928 1525 1450 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3138 6434 3138
Size: 3296 3143
BlpI GC'TnA_GC
Cuts at: 2162 2660 3856 2162
Size: 498 1196 4745
Fragments arranged by size:
4745 1196 498
Bme1580I G_kGCm'C
Cuts at: 1790 2673 2688 2782 3014 4500 5656 1790
Size: 883 15 94 232 1486 1156 2573
Fragments arranged by size:
2573 1486 1156 883 232 94 15
BmgBI CAC'GTC
Cuts at: 1318 2239 3169 1318
Size: 921 930 4588
Fragments arranged by size:
4588 930 921
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3545 4170 5128
Size: 416 53 83 399 24 2128 625 958
Cuts at: 5128 442
Size: 1753
Fragments arranged by size:
2128 1753 958 625 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6439
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 6439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2777 2825 2861 3274 5099 1239
Size: 1538 48 36 413 1825 2579
Fragments arranged by size:
2579 1825 1538 413 48 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4564 5429 5727 4564
Size: 865 298 5276
Fragments arranged by size:
5276 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5102 882
Size: 617 3603 2219
Fragments arranged by size:
3603 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2504 3457 3553 3595 4230 493
Size: 711 1300 953 96 42 635 2702
Fragments arranged by size:
2702 1300 953 711 635 96 42
BsaBI GATnn'nnATC
Cuts at: 6433 6433
Size: 6439
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2106 2679 3086 3212
Size: 53 83 186 936 573 573 407 126
Cuts at: 3212 3645 4246 4628 5263 275
Size: 433 601 382 635 1451
Fragments arranged by size:
1451 936 635 601 573 573 433 407
382 186 126 83 53
BsaWI w'CCGG_w
Cuts at: 4872 5270 5544 5691 6102 4872
Size: 398 274 147 411 5209
Fragments arranged by size:
5209 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2775 2805 3662 3692 1178
Size: 30 165 30 1372 30 857 30 3925
Fragments arranged by size:
3925 1372 857 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2822 2858 2861 2864 2876 1186
Size: 1636 36 3 3 12 4749
Fragments arranged by size:
4749 1636 36 12 3 3
BseYI C'CCAG_C
Cuts at: 2446 2610 3711 5642 6138 2446
Size: 164 1101 1931 496 2747
Fragments arranged by size:
2747 1931 1101 496 164
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2327 2378 2720 3390 3395 3512 1163
Size: 573 591 51 342 670 5 117 4090
Fragments arranged by size:
4090 670 591 573 342 117 51 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2128 2374 3565 3889 4650 5678
Size: 275 186 127 246 1191 324 761 1028
Cuts at: 5678 1540
Size: 2301
Fragments arranged by size:
2301 1191 1028 761 324 275 246 186
127
BsiHKAI G_wGCw'C
Cuts at: 2673 3423 3738 4500 4585 5656 2673
Size: 750 315 762 85 1071 3456
Fragments arranged by size:
3456 1071 762 750 315 85
BsmI GAATG_Cn'
Cuts at: 3950 4043 3950
Size: 93 6346
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 2673 2688 2724 2782 2868 3014
Size: 399 457 426 15 36 58 86 146
Cuts at: 3014 3290 3423 3738 3758 4500 4585 5656 1391
Size: 276 133 315 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 457 426 399 315 276
146 133 86 85 58 36 20 15
BspEI T'CCGG_A
Cuts at: 6102 6102
Size: 6439
BspHI T'CATG_A
Cuts at: 4328 6058 4328
Size: 1730 4709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2046 2388 2560 2571 2655 2846 3794 844
Size: 1202 342 172 11 84 191 948 3489
Fragments arranged by size:
3489 1202 948 342 191 172 84 11
BsrBI CCG'CTC
Cuts at: 1394 1823 2536 4326 1394
Size: 429 713 1790 3507
Fragments arranged by size:
3507 1790 713 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4934 5108 65
Size: 1183 3686 174 1396
Fragments arranged by size:
3686 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2286 2555 3257 3270 5083 5270 1700
Size: 110 476 269 702 13 1813 187 2869
Fragments arranged by size:
2869 1813 702 476 269 187 110 13
BsrGI T'GTAC_A
Cuts at: 96 2277 96
Size: 2181 4258
BssHII G'CGCG_C
Cuts at: 1925 2219 2336 3522 1925
Size: 294 117 1186 4842
Fragments arranged by size:
4842 1186 294 117
BssSI C'ACGA_G
Cuts at: 4499 5511 4499
Size: 1012 5427
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2080 2443 2707 3490 3508 794
Size: 1286 363 264 783 18 3725
Fragments arranged by size:
3725 1286 783 363 264 18
BstBI TT'CG_AA
Cuts at: 5243 5243
Size: 6439
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6439
BstXI CCAn_nnnn'nTGG
Cuts at: 2410 2410
Size: 6439
BstYI r'GATC_y
Cuts at: 1972 3138 3769 4136 4521 4538 5990 6076 6088
Size: 1166 631 367 385 17 1452 86 12
Cuts at: 6088 6434 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1166 631 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2250 3081 3640 3670 513
Size: 552 939 246 831 559 30 3282
Fragments arranged by size:
3282 939 831 559 552 246 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5700
Fragments arranged by size:
5700 683 56
BtsI GCAGTG_nn'
Cuts at: 2111 3329 3407 3955 4749 4769 2111
Size: 1218 78 548 794 20 3781
Fragments arranged by size:
3781 1218 794 548 78 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6439
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6404
DraI TTT'AAA
Cuts at: 3743 3743
Size: 6439
DraIII CAC_nnn'GTG
Cuts at: 3539 3539
Size: 6439
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5446 809
Size: 507 4130 1802
Fragments arranged by size:
4130 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2125 2371
Size: 54 1425 50 275 18 270 25 246
Cuts at: 2371 2553 3562 3886 4775 6404 8
Size: 182 1009 324 889 1629 43
Fragments arranged by size:
1629 1425 1009 889 324 275 270 246
182 54 50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 2125 2371 3562 3886 1537
Size: 275 313 246 1191 324 4090
Fragments arranged by size:
4090 1191 324 313 275 246
EarI CTCTTCn'nnn_
Cuts at: 1353 4369 1353
Size: 3016 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5026 5400 5546 387
Size: 1064 138 3437 374 146 1280
Fragments arranged by size:
3437 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3421 3421
Size: 6439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2777 2825 2861 3080 3116 3274 4502
Size: 393 1145 48 36 219 36 158 1228
Cuts at: 4502 5099 5886 6154 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1228 1145 787 597 393 268 219
158 48 36 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2567 2729 2762 3062 2567
Size: 162 33 300 5944
Fragments arranged by size:
5944 300 162 33
EcoO109I rG'GnC_Cy
Cuts at: 2599 2691 2812 3675 2599
Size: 92 121 863 5363
Fragments arranged by size:
5363 863 121 92
EcoRI G'AATT_C
Cuts at: 3748 4126 3748
Size: 378 6061
FspI TGC'GCA
Cuts at: 4945 4945
Size: 6439
HaeII r_GCGC'y
Cuts at: 1678 2080 2162 2321 2840 3089 3297 3648 5586
Size: 402 82 159 519 249 208 351 1938
Cuts at: 5586 1678
Size: 2531
Fragments arranged by size:
2531 1938 519 402 351 249 208 159
82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3014 3046 5080 5112 5154
Size: 32 135 32 1442 32 2034 32 42
Cuts at: 5154 5186 1373
Size: 32 2626
Fragments arranged by size:
2626 2034 1442 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3783 4029 669
Size: 988 281 1095 750 246 3079
Fragments arranged by size:
3079 1095 988 750 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6439
HpaI GTT'AAC
Cuts at: 4029 4029
Size: 6439
KasI G'GCGC_C
Cuts at: 3085 3644 3085
Size: 559 5880
KpnI G_GTAC'C
Cuts at: 3764 3764
Size: 6439
MfeI C'AATT_G
Cuts at: 4038 4038
Size: 6439
MluI A'CGCG_T
Cuts at: 6315 6315
Size: 6439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5553 5737 1305
Size: 4248 184 2007
Fragments arranged by size:
4248 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4617
Fragments arranged by size:
4617 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4397 4756 4915 518
Size: 648 3231 359 159 2042
Fragments arranged by size:
3231 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3207 3567 3855 4532 5680 5925 1606
Size: 1601 360 288 677 1148 245 2120
Fragments arranged by size:
2120 1601 1148 677 360 288 245
NaeI GCC'GGC
Cuts at: 2288 2557 3259 3272 2288
Size: 269 702 13 5455
Fragments arranged by size:
5455 702 269 13
NarI GG'CG_CC
Cuts at: 3086 3645 3086
Size: 559 5880
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4948
Fragments arranged by size:
4948 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6439
NgoMIV G'CCGG_C
Cuts at: 2286 2555 3257 3270 2286
Size: 269 702 13 5455
Fragments arranged by size:
5455 702 269 13
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6439
NotI GC'GGCC_GC
Cuts at: 2371 3562 3886 2371
Size: 1191 324 4924
Fragments arranged by size:
4924 1191 324
NspI r_CATG'y
Cuts at: 1132 1229 2051 2062 2748 3797 5342 1132
Size: 97 822 11 686 1049 1545 2229
Fragments arranged by size:
2229 1545 1049 822 686 97 11
PasI CC'CwG_GG
Cuts at: 1348 2764 1348
Size: 1416 5023
PciI A'CATG_T
Cuts at: 5338 5338
Size: 6439
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2466 2916 3480 1108
Size: 1358 450 564 4067
Fragments arranged by size:
4067 1358 564 450
PfoI T'CCnGG_A
Cuts at: 2730 3071 3690 2730
Size: 341 619 5479
Fragments arranged by size:
5479 619 341
PmeI GTTT'AAAC
Cuts at: 3743 3743
Size: 6439
PmlI CAC'GTG
Cuts at: 2504 3595 2504
Size: 1091 5348
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2498 2530 4508 4540 6021 6053 2498
Size: 32 1978 32 1481 32 2884
Fragments arranged by size:
2884 1978 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2812 2812
Size: 6439
PshAI GACnn'nnGTC
Cuts at: 1613 3215 1613
Size: 1602 4837
PsiI TTA'TAA
Cuts at: 4009 4009
Size: 6439
PspXI vC'TCGA_Gb
Cuts at: 2594 2594
Size: 6439
PstI C_TGCA'G
Cuts at: 830 2076 2385 2523 2571 3324 3791 830
Size: 1246 309 138 48 753 467 3478
Fragments arranged by size:
3478 1246 753 467 309 138 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6439
PvuII CAG'CTG
Cuts at: 3207 3207
Size: 6439
SacI G_AGCT'C
Cuts at: 3423 3423
Size: 6439
SalI G'TCGA_C
Cuts at: 1936 3781 1936
Size: 1845 4594
SbfI CC_TGCA'GG
Cuts at: 3791 3791
Size: 6439
ScaI AGT'ACT
Cuts at: 4687 6399 4687
Size: 1712 4727
SexAI A'CCwGG_T
Cuts at: 3026 3279 3026
Size: 253 6186
SfcI C'TryA_G
Cuts at: 826 1045 2072 2381 2519 2567 3320 3787 4922
Size: 219 1027 309 138 48 753 467 1135
Cuts at: 4922 5603 5794 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1027 753 681 467 309 219
191 138 48
SfoI GGC'GCC
Cuts at: 3087 3646 3087
Size: 559 5880
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6439
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6439
SmaI CCC'GGG
Cuts at: 1872 2636 3469 3766 1872
Size: 764 833 297 4545
Fragments arranged by size:
4545 833 764 297
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2594 4543 5444 5706 820
Size: 197 926 651 1949 901 262 1553
Fragments arranged by size:
1949 1553 926 901 651 262 197
SnaBI TAC'GTA
Cuts at: 493 4230 493
Size: 3737 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6439
SphI G_CATG'C
Cuts at: 1132 2051 2748 3797 1132
Size: 919 697 1049 3774
Fragments arranged by size:
3774 1049 919 697
SrfI GCCC'GGGC
Cuts at: 2636 2636
Size: 6439
SspI AAT'ATT
Cuts at: 5 52 4363 5
Size: 47 4311 2081
Fragments arranged by size:
4311 2081 47
StuI AGG'CCT
Cuts at: 1517 2465 3044 3068 3608 1517
Size: 948 579 24 540 4348
Fragments arranged by size:
4348 948 579 540 24
StyI C'CwwG_G
Cuts at: 513 1065 2004 2460 2878 3878 513
Size: 552 939 456 418 1000 3074
Fragments arranged by size:
3074 1000 939 552 456 418
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2602 3053 3527 3743 4466 4483 4636
Size: 1255 1022 451 474 216 723 17 153
Cuts at: 4636 4821 5266 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1022 723 474 451 445 216
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 3303 4685
Size: 274 80 33 51 1443 300 1026 1382
Cuts at: 4685 6397 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1382 1026 300 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2250 2282 2775 2807 3662 3694 6399 6431 2250
Size: 32 493 32 855 32 2705 32 2258
Fragments arranged by size:
2705 2258 855 493 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6439
XbaI T'CTAG_A
Cuts at: 3775 3775
Size: 6439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2771 2917 3166 3688 2771
Size: 146 249 522 5522
Fragments arranged by size:
5522 522 249 146
XhoI C'TCGA_G
Cuts at: 1943 2594 1943
Size: 651 5788
XmaI C'CCGG_G
Cuts at: 1870 2634 3467 3764 1870
Size: 764 833 297 4545
Fragments arranged by size:
4545 833 764 297
XmnI GAAnn'nnTTC
Cuts at: 4568 4568
Size: 6439
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2107 4247 276
Size: 53 83 186 936 573 2140 2468
Fragments arranged by size:
2468 2140 936 573 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI
FspAI NruI NsiI PacI PspOMI PsrI RsrII SacII
SanDI SapI SfiI SwaI