Restriction map for
pFN21AE4144
(Circular) (Six-base) MAPSORT of: pFN21AE4144.seq Check: 3616 from: 1 to: 6421
pFN21AE4144 6421 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3346 3346
Size: 6421
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4231 278
Size: 53 83 186 936 2695 2468
Fragments arranged by size:
2695 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3764 1937
Size: 1827 4594
Acc65I G'GTAC_C
Cuts at: 3742 3742
Size: 6421
AclI AA'CG_TT
Cuts at: 1563 4548 4921 1563
Size: 2985 373 3063
Fragments arranged by size:
3063 2985 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2331 3158 4484 5868 6136 1632
Size: 699 827 1326 1384 268 1917
Fragments arranged by size:
1917 1384 1326 827 699 268
AfeI AGC'GCT
Cuts at: 2032 3601 2032
Size: 1569 4852
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6224
AflIII A'CryG_T
Cuts at: 1203 5320 6297 1203
Size: 4117 977 1327
Fragments arranged by size:
4117 1327 977
AgeI A'CCGG_T
Cuts at: 5252 5252
Size: 6421
AhdI GACnn_n'nnGTC
Cuts at: 3665 5150 3665
Size: 1485 4936
AleI CACnn'nnGTG
Cuts at: 1166 3431 1166
Size: 2265 4156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2109 1184
Size: 925 5496
AlwNI CAG_nnn'CTG
Cuts at: 2324 4164 5736 2324
Size: 1840 1572 3009
Fragments arranged by size:
3009 1840 1572
ApaI G_GGCC'C
Cuts at: 2921 2921
Size: 6421
ApaLI G'TGCA_C
Cuts at: 4478 5634 4478
Size: 1156 5265
ApoI r'AATT_y
Cuts at: 1493 2246 2334 3730 3956 4108 6217 1493
Size: 753 88 1396 226 152 2109 1697
Fragments arranged by size:
2109 1697 1396 753 226 152 88
AseI AT'TA_AT
Cuts at: 160 1032 4975 160
Size: 872 3943 1606
Fragments arranged by size:
3943 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
AvaI C'yCGr_G
Cuts at: 1870 1943 3746 1870
Size: 73 1803 4545
Fragments arranged by size:
4545 1803 73
AvrII C'CTAG_G
Cuts at: 2786 2786
Size: 6421
BamHI G'GATC_C
Cuts at: 3751 4118 3751
Size: 367 6054
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3085 3742 5197 618
Size: 325 217 627 1298 657 1455 1842
Fragments arranged by size:
1842 1455 1298 657 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2921 3055 3377 3740 1391
Size: 1530 134 322 363 4072
Fragments arranged by size:
4072 1530 363 322 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3573 928
Size: 971 1674 3776
Fragments arranged by size:
3776 1674 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4612 4646 1903
Size: 34 2675 34 3678
Fragments arranged by size:
3678 2675 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2153 4313 5523 2153
Size: 2160 1210 3051
Fragments arranged by size:
3051 2160 1210
BclI T'GATC_A
Cuts at: 1548 2829 1548
Size: 1281 5140
BfrBI ATG'CAT
Cuts at: 2657 2657
Size: 6421
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5032 136
Size: 107 122 71 631 605 3360 1525
Fragments arranged by size:
3360 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6416 6416
Size: 6421
BlpI GC'TnA_GC
Cuts at: 3838 3838
Size: 6421
Bme1580I G_kGCm'C
Cuts at: 1790 2921 4482 5638 1790
Size: 1131 1561 1156 2573
Fragments arranged by size:
2573 1561 1156 1131
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6421
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4152 5110 442
Size: 416 53 83 399 24 2735 958 1753
Fragments arranged by size:
2735 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6421
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3459 3459
Size: 6421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2670 3061 3324 5081 1239
Size: 1431 391 263 1757 2579
Fragments arranged by size:
2579 1757 1431 391 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4546 5411 5709 4546
Size: 865 298 5258
Fragments arranged by size:
5258 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5084 882
Size: 617 3585 2219
Fragments arranged by size:
3585 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3166 4212 493
Size: 711 1962 1046 2702
Fragments arranged by size:
2702 1962 1046 711
BsaBI GATnn'nnATC
Cuts at: 6415 6415
Size: 6421
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3063 4228 4610 5245
Size: 53 83 186 936 1530 1165 382 635
Cuts at: 5245 275
Size: 1451
Fragments arranged by size:
1530 1451 1165 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2387 2923 3377 3625 4854 5252 5526 5673 6084
Size: 536 454 248 1229 398 274 147 411
Cuts at: 6084 2387
Size: 2724
Fragments arranged by size:
2724 1229 536 454 411 398 274 248
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3349 3364 3379 3394 1178
Size: 30 165 30 1946 15 15 15 4205
Fragments arranged by size:
4205 1946 165 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2559 2775 3597 1186
Size: 1373 216 822 4010
Fragments arranged by size:
4010 1373 822 216
BseYI C'CCAG_C
Cuts at: 3237 3465 5624 6120 3237
Size: 228 2159 496 3538
Fragments arranged by size:
3538 2159 496 228
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 2057 1163
Size: 573 303 18 5527
Fragments arranged by size:
5527 573 303 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3871 4632 5660 1540
Size: 275 186 1870 761 1028 2301
Fragments arranged by size:
2301 1870 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3301 3377 4482 4567 5638 3301
Size: 76 1105 85 1071 4084
Fragments arranged by size:
4084 1105 1071 85 76
BsmI GAATG_Cn'
Cuts at: 3932 4025 3932
Size: 93 6328
BsmBI CGTCTCn'nnnn_
Cuts at: 2979 2979
Size: 6421
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2921 3055 3301 3377 3740 4482 4567
Size: 399 1131 134 246 76 363 742 85
Cuts at: 4567 5638 1391
Size: 1071 2174
Fragments arranged by size:
2174 1131 1071 742 399 363 246 134
85 76
BspEI T'CCGG_A
Cuts at: 2923 6084 2923
Size: 3161 3260
BspHI T'CATG_A
Cuts at: 2595 4310 6040 2595
Size: 1715 1730 2976
Fragments arranged by size:
2976 1730 1715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3216 3346 3496 3655 3709 3776 844
Size: 2372 130 150 159 54 67 3489
Fragments arranged by size:
3489 2372 159 150 130 67 54
BsrBI CCG'CTC
Cuts at: 1394 1823 2575 4308 1394
Size: 429 752 1733 3507
Fragments arranged by size:
3507 1733 752 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2612 4916 5090 65
Size: 1183 1364 2304 174 1396
Fragments arranged by size:
2304 1396 1364 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5065 5252 1700
Size: 110 3255 187 2869
Fragments arranged by size:
3255 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6421
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6421
BssSI C'ACGA_G
Cuts at: 4481 5493 4481
Size: 1012 5409
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6421
BstBI TT'CG_AA
Cuts at: 5225 5225
Size: 6421
BstEII G'GTnAC_C
Cuts at: 1184 2198 1184
Size: 1014 5407
BstYI r'GATC_y
Cuts at: 1972 2838 3751 4118 4503 4520 5972 6058 6070
Size: 866 913 367 385 17 1452 86 12
Cuts at: 6070 6416 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 913 866 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3609 3609
Size: 6421
BtgI C'CryG_G
Cuts at: 513 1065 2004 2619 3031 513
Size: 552 939 615 412 3903
Fragments arranged by size:
3903 939 615 552 412
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5682
Fragments arranged by size:
5682 683 56
BtsI GCAGTG_nn'
Cuts at: 3937 4731 4751 3937
Size: 794 20 5607
Fragments arranged by size:
5607 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6421
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6386
DraI TTT'AAA
Cuts at: 2662 3725 2662
Size: 1063 5358
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5428 809
Size: 507 4112 1802
Fragments arranged by size:
4112 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3028 3252 3868
Size: 54 1425 50 275 18 1198 224 616
Cuts at: 3868 4757 6386 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1198 889 616 275 224 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3868 1537
Size: 275 2056 4090
Fragments arranged by size:
4090 2056 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2643 3013 4351 1353
Size: 1290 370 1338 3423
Fragments arranged by size:
3423 1338 1290 370
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5008 5382 5528 387
Size: 1064 138 435 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 3375 3375
Size: 6421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2331 2670 3061 3158 3324 4484 5081
Size: 393 699 339 391 97 166 1160 597
Cuts at: 5081 5868 6136 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1160 787 699 597 393 391 339
268 166 97
EcoO109I rG'GnC_Cy
Cuts at: 2211 2373 2918 3508 2211
Size: 162 545 590 5124
Fragments arranged by size:
5124 590 545 162
EcoRI G'AATT_C
Cuts at: 2246 3730 4108 2246
Size: 1484 378 4559
Fragments arranged by size:
4559 1484 378
FspI TGC'GCA
Cuts at: 4927 4927
Size: 6421
HaeII r_GCGC'y
Cuts at: 1678 2034 3603 5568 1678
Size: 356 1569 1965 2531
Fragments arranged by size:
2531 1965 1569 356
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2265 2297 2732 2764 3207
Size: 32 135 32 693 32 435 32 443
Cuts at: 3207 3239 3651 3683 5062 5094 5136 5168 1373
Size: 32 412 32 1379 32 42 32 2626
Fragments arranged by size:
2626 1379 693 443 435 412 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3765 4011 669
Size: 988 281 1827 246 3079
Fragments arranged by size:
3079 1827 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6421
HpaI GTT'AAC
Cuts at: 4011 4011
Size: 6421
KpnI G_GTAC'C
Cuts at: 3746 3746
Size: 6421
MfeI C'AATT_G
Cuts at: 4020 4020
Size: 6421
MluI A'CGCG_T
Cuts at: 6297 6297
Size: 6421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2251 5535 5719 1305
Size: 946 3284 184 2007
Fragments arranged by size:
3284 2007 946 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3254 10
Size: 54 1425 343 1422 3177
Fragments arranged by size:
3177 1425 1422 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3431 4379 4738 4897 518
Size: 648 2265 948 359 159 2042
Fragments arranged by size:
2265 2042 948 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2445 3837 4514 5662 5907 1606
Size: 839 1392 677 1148 245 2120
Fragments arranged by size:
2120 1392 1148 839 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4930
Fragments arranged by size:
4930 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6421
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6421
NotI GC'GGCC_GC
Cuts at: 3868 3868
Size: 6421
NsiI A_TGCA'T
Cuts at: 2659 2659
Size: 6421
NspI r_CATG'y
Cuts at: 1132 1229 2942 3779 5324 1132
Size: 97 1713 837 1545 2229
Fragments arranged by size:
2229 1713 1545 837 97
PasI CC'CwG_GG
Cuts at: 1348 3460 1348
Size: 2112 4309
PciI A'CATG_T
Cuts at: 5320 5320
Size: 6421
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2882 2914 1108
Size: 1774 32 4615
Fragments arranged by size:
4615 1774 32
PfoI T'CCnGG_A
Cuts at: 2496 2601 3066 2496
Size: 105 465 5851
Fragments arranged by size:
5851 465 105
PmeI GTTT'AAAC
Cuts at: 3725 3725
Size: 6421
PmlI CAC'GTG
Cuts at: 3166 3166
Size: 6421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4490 4522 6003 6035 4490
Size: 32 1481 32 4876
Fragments arranged by size:
4876 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3508 3508
Size: 6421
PshAI GACnn'nnGTC
Cuts at: 1613 2445 1613
Size: 832 5589
PsiI TTA'TAA
Cuts at: 3991 3991
Size: 6421
PspOMI G'GGCC_C
Cuts at: 2917 2917
Size: 6421
PstI C_TGCA'G
Cuts at: 830 3773 830
Size: 2943 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6421
SacI G_AGCT'C
Cuts at: 3377 3377
Size: 6421
SalI G'TCGA_C
Cuts at: 1936 3763 1936
Size: 1827 4594
SbfI CC_TGCA'GG
Cuts at: 3773 3773
Size: 6421
ScaI AGT'ACT
Cuts at: 4669 6381 4669
Size: 1712 4709
SfcI C'TryA_G
Cuts at: 826 1045 3550 3769 4904 5585 5776 826
Size: 219 2505 219 1135 681 191 1471
Fragments arranged by size:
2505 1471 1135 681 219 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6421
SmaI CCC'GGG
Cuts at: 1872 3748 1872
Size: 1876 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4525 5426 5688 820
Size: 197 926 2582 901 262 1553
Fragments arranged by size:
2582 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4212 493
Size: 3719 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6421
SphI G_CATG'C
Cuts at: 1132 2942 3779 1132
Size: 1810 837 3774
Fragments arranged by size:
3774 1810 837
SspI AAT'ATT
Cuts at: 5 52 3653 4345 5
Size: 47 3601 692 2081
Fragments arranged by size:
3601 2081 692 47
StuI AGG'CCT
Cuts at: 1517 3000 3560 1517
Size: 1483 560 4378
Fragments arranged by size:
4378 1483 560
StyI C'CwwG_G
Cuts at: 513 1065 2004 2181 2214 2786 3368 3402 3860
Size: 552 939 177 33 572 582 34 458
Cuts at: 3860 513
Size: 3074
Fragments arranged by size:
3074 939 582 572 552 458 177 34
33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4448 4465 4618 4803 5248 325
Size: 1255 2868 17 153 185 445 1498
Fragments arranged by size:
2868 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2167 2380 2665
Size: 274 80 33 51 1443 190 213 285
Cuts at: 2665 4667 6379 96
Size: 2002 1712 138
Fragments arranged by size:
2002 1712 1443 285 274 213 190 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2421 2453 3492 3524 6381 6413 2421
Size: 32 1039 32 2857 32 2429
Fragments arranged by size:
2857 2429 1039 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3318 1316
Size: 2002 4419
XbaI T'CTAG_A
Cuts at: 3757 3757
Size: 6421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2137 2422 2849 2011
Size: 126 285 427 5583
Fragments arranged by size:
5583 427 285 126
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6421
XmaI C'CCGG_G
Cuts at: 1870 3746 1870
Size: 1876 4545
XmnI GAAnn'nnTTC
Cuts at: 4550 4550
Size: 6421
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4229 276
Size: 53 83 186 936 2695 2468
Fragments arranged by size:
2695 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BbvCI Bpu10I BsiWI BstXI
BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI