(Circular) (Six-base) MAPSORT of: pFN21AE4137.seq Check: 2310 from: 1 to: 6061 pFN21AE4137 6061 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2441 2600 3223 2441 Size: 159 623 5279 Fragments arranged by size: 5279 623 159 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2248 3404 1937 Size: 311 1156 4594 Fragments arranged by size: 4594 1156 311 Acc65I G'GTAC_C Cuts at: 2204 3382 2204 Size: 1178 4883 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 3117 4124 5508 5776 1632 Size: 641 844 1007 1384 268 1917 Fragments arranged by size: 1917 1384 1007 844 641 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 2731 4960 5937 1203 Size: 1528 2229 977 1327 Fragments arranged by size: 2229 1528 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 2492 1166 Size: 1326 4735 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AlwNI CAG_nnn'CTG Cuts at: 3804 5376 3804 Size: 1572 4489 ApaLI G'TGCA_C Cuts at: 2457 4118 5274 2457 Size: 1661 1156 3244 Fragments arranged by size: 3244 1661 1156 ApoI r'AATT_y Cuts at: 1493 2799 3370 3596 3748 5857 1493 Size: 1306 571 226 152 2109 1697 Fragments arranged by size: 2109 1697 1306 571 226 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 3386 1870 Size: 73 1443 4545 Fragments arranged by size: 4545 1443 73 BamHI G'GATC_C Cuts at: 3391 3758 3391 Size: 367 5694 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2204 3382 4837 618 Size: 325 217 627 417 1178 1455 1842 Fragments arranged by size: 1842 1455 1178 627 417 325 217 BanII G_rGCy'C Cuts at: 1391 2520 3141 3380 1391 Size: 1129 621 239 4072 Fragments arranged by size: 4072 1129 621 239 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2592 928 Size: 971 693 4397 Fragments arranged by size: 4397 971 693 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2217 2251 2584 2618 4252 4286 1903 Size: 34 280 34 333 34 1634 34 3678 Fragments arranged by size: 3678 1634 333 280 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 2571 2745 2761 1548 Size: 1023 174 16 4848 Fragments arranged by size: 4848 1023 174 16 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3170 4672 136 Size: 107 122 71 631 605 1498 1502 1525 Fragments arranged by size: 1525 1502 1498 631 605 122 107 71 BglII A'GATC_T Cuts at: 6056 6056 Size: 6061 BlpI GC'TnA_GC Cuts at: 3478 3478 Size: 6061 Bme1580I G_kGCm'C Cuts at: 1790 2461 3175 4122 5278 1790 Size: 671 714 947 1156 2573 Fragments arranged by size: 2573 1156 947 714 671 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3792 4750 442 Size: 416 53 83 399 24 2375 958 1753 Fragments arranged by size: 2375 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2031 2794 2031 Size: 763 5298 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3174 3186 3248 3267 4721 1239 Size: 1935 12 62 19 1454 2579 Fragments arranged by size: 2579 1935 1454 62 19 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4186 5051 5349 4186 Size: 865 298 4898 Fragments arranged by size: 4898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4724 882 Size: 617 3225 2219 Fragments arranged by size: 3225 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2969 3852 493 Size: 711 1765 883 2702 Fragments arranged by size: 2702 1765 883 711 BsaBI GATnn'nnATC Cuts at: 6055 6055 Size: 6061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3868 4250 4885 275 Size: 53 83 186 936 2335 382 635 1451 Fragments arranged by size: 2335 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4494 4892 5166 5313 5724 4494 Size: 398 274 147 411 4831 Fragments arranged by size: 4831 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3048 3078 1178 Size: 30 165 30 1645 30 4161 Fragments arranged by size: 4161 1645 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2214 2405 2687 2732 3069 3246 1186 Size: 1028 191 282 45 337 177 4001 Fragments arranged by size: 4001 1028 337 282 191 177 45 BseYI C'CCAG_C Cuts at: 5264 5760 5264 Size: 496 5565 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5488 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2557 3511 4272 5300 1540 Size: 275 186 556 954 761 1028 2301 Fragments arranged by size: 2301 1028 954 761 556 275 186 BsiHKAI G_wGCw'C Cuts at: 2461 2520 2743 3141 4122 4207 5278 2461 Size: 59 223 398 981 85 1071 3244 Fragments arranged by size: 3244 1071 981 398 223 85 59 BsmI GAATG_Cn' Cuts at: 2848 3572 3665 2848 Size: 724 93 5244 Fragments arranged by size: 5244 724 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2782 2782 Size: 6061 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2461 2520 2743 3141 3175 3380 4122 Size: 399 671 59 223 398 34 205 742 Cuts at: 4122 4207 5278 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 671 399 398 223 205 85 59 34 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 3950 5680 3950 Size: 1730 4331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2307 2441 2600 3223 3416 844 Size: 1463 134 159 623 193 3489 Fragments arranged by size: 3489 1463 623 193 159 134 BsrBI CCG'CTC Cuts at: 1394 1823 3948 1394 Size: 429 2125 3507 Fragments arranged by size: 3507 2125 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4556 4730 65 Size: 1183 3308 174 1396 Fragments arranged by size: 3308 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4705 4892 1700 Size: 110 2895 187 2869 Fragments arranged by size: 2895 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6061 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 2540 4121 5133 2540 Size: 1581 1012 3468 Fragments arranged by size: 3468 1581 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6061 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstXI CCAn_nnnn'nTGG Cuts at: 2542 2542 Size: 6061 BstYI r'GATC_y Cuts at: 1972 3391 3758 4143 4160 5612 5698 5710 6056 Size: 1419 367 385 17 1452 86 12 346 Cuts at: 6056 1972 Size: 1977 Fragments arranged by size: 1977 1452 1419 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2052 2211 513 Size: 552 987 159 4363 Fragments arranged by size: 4363 987 552 159 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2515 487 Size: 683 56 1289 4033 Fragments arranged by size: 4033 1289 683 56 BtsI GCAGTG_nn' Cuts at: 2835 3301 3577 4371 4391 2835 Size: 466 276 794 20 4505 Fragments arranged by size: 4505 794 466 276 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3508 4397 6026 Size: 54 1425 50 275 18 1678 889 1629 Cuts at: 6026 8 Size: 43 Fragments arranged by size: 1678 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3508 1537 Size: 275 1696 4090 Fragments arranged by size: 4090 1696 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2382 3069 3991 1353 Size: 1029 687 922 3423 Fragments arranged by size: 3423 1029 922 687 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3033 4648 5022 5168 387 Size: 1064 138 1444 1615 374 146 1280 Fragments arranged by size: 1615 1444 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2518 3139 2518 Size: 621 5440 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 3117 3174 3186 3248 3267 4124 Size: 393 641 844 57 12 62 19 857 Cuts at: 4124 4721 5508 5776 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 857 844 787 641 597 393 268 62 57 19 12 EcoRI G'AATT_C Cuts at: 2799 3370 3748 2799 Size: 571 378 5112 Fragments arranged by size: 5112 571 378 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 5208 1678 Size: 3530 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4702 4734 4776 4808 1373 Size: 32 135 32 3130 32 42 32 2626 Fragments arranged by size: 3130 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2941 3405 3651 669 Size: 988 281 1003 464 246 3079 Fragments arranged by size: 3079 1003 988 464 281 246 HindIII A'AGCT_T Cuts at: 748 3129 748 Size: 2381 3680 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KpnI G_GTAC'C Cuts at: 2208 3386 2208 Size: 1178 4883 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2385 5175 5359 1305 Size: 1080 2790 184 2007 Fragments arranged by size: 2790 2007 1080 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4239 Fragments arranged by size: 4239 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2492 2540 2736 2913 4019 4378 4537 Size: 648 1326 48 196 177 1106 359 159 Cuts at: 4537 518 Size: 2042 Fragments arranged by size: 2042 1326 1106 648 359 196 177 159 48 MspA1I CmG'CkG Cuts at: 1606 2235 2581 3477 4154 5302 5547 1606 Size: 629 346 896 677 1148 245 2120 Fragments arranged by size: 2120 1148 896 677 629 346 245 NcoI C'CATG_G Cuts at: 513 1065 2052 2211 513 Size: 552 987 159 4363 Fragments arranged by size: 4363 987 552 159 NdeI CA'TA_TG Cuts at: 387 387 Size: 6061 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 2735 3419 4964 1132 Size: 97 1506 684 1545 2229 Fragments arranged by size: 2229 1545 1506 684 97 PasI CC'CwG_GG Cuts at: 1348 3167 1348 Size: 1819 4242 PciI A'CATG_T Cuts at: 2731 4960 2731 Size: 2229 3832 PflMI CCAn_nnn'nTGG Cuts at: 1108 3290 1108 Size: 2182 3879 PfoI T'CCnGG_A Cuts at: 3103 3103 Size: 6061 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2509 2696 1613 Size: 896 187 4978 Fragments arranged by size: 4978 896 187 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3072 3104 3072 Size: 32 6029 PstI C_TGCA'G Cuts at: 830 2841 3159 3280 3413 830 Size: 2011 318 121 133 3478 Fragments arranged by size: 3478 2011 318 133 121 PvuI CG_AT'CG Cuts at: 2001 2557 2001 Size: 556 5505 PvuII CAG'CTG Cuts at: 2581 2581 Size: 6061 SacI G_AGCT'C Cuts at: 2520 3141 2520 Size: 621 5440 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SfcI C'TryA_G Cuts at: 826 1045 2245 2837 3155 3276 3409 4544 5225 Size: 219 1200 592 318 121 133 1135 681 Cuts at: 5225 5416 826 Size: 191 1471 Fragments arranged by size: 1471 1200 1135 681 592 318 219 191 133 121 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 3388 1872 Size: 1516 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4165 5066 5328 820 Size: 197 926 2222 901 262 1553 Fragments arranged by size: 2222 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 2174 1517 Size: 657 5404 StyI C'CwwG_G Cuts at: 513 1065 2052 2211 3500 513 Size: 552 987 159 1289 3074 Fragments arranged by size: 3074 1289 987 552 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4088 4105 4258 4443 4888 325 Size: 1255 2508 17 153 185 445 1498 Fragments arranged by size: 2508 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4307 6019 96 Size: 274 80 33 51 1443 2330 1712 138 Fragments arranged by size: 2330 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6021 6053 6021 Size: 32 6029 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6061 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 3386 1870 Size: 1516 4545 XmnI GAAnn'nnTTC Cuts at: 2930 3087 4190 2930 Size: 157 1103 4801 Fragments arranged by size: 4801 1103 157 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI