Restriction map for
pFN21AE4134
(Circular) (Six-base) MAPSORT of: pFN21AE4134.seq Check: 1729 from: 1 to: 6322
pFN21AE4134 6322 bp
With 182 enzymes: SgfI *
January 19, 2012 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2628 2628
Size: 6322
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2133 4132 278
Size: 53 83 186 936 597 1999 2468
Fragments arranged by size:
2468 1999 936 597 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3108 3665 1937
Size: 1171 557 4594
Fragments arranged by size:
4594 1171 557
Acc65I G'GTAC_C
Cuts at: 2976 3643 2976
Size: 667 5655
AclI AA'CG_TT
Cuts at: 1563 4449 4822 1563
Size: 2886 373 3063
Fragments arranged by size:
3063 2886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 2558 4385 5769 6037 1632
Size: 635 291 1827 1384 268 1917
Fragments arranged by size:
1917 1827 1384 635 291 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6125
AflIII A'CryG_T
Cuts at: 1203 5221 6198 1203
Size: 4018 977 1327
Fragments arranged by size:
4018 1327 977
AgeI A'CCGG_T
Cuts at: 5153 5153
Size: 6322
AhdI GACnn_n'nnGTC
Cuts at: 2962 5051 2962
Size: 2089 4233
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2313 2323 2769 1184
Size: 1129 10 446 4737
Fragments arranged by size:
4737 1129 446 10
AlwNI CAG_nnn'CTG
Cuts at: 2599 4065 5637 2599
Size: 1466 1572 3284
Fragments arranged by size:
3284 1572 1466
ApaLI G'TGCA_C
Cuts at: 4379 5535 4379
Size: 1156 5166
ApoI r'AATT_y
Cuts at: 1493 2490 2858 2900 3631 3857 4009 6118 1493
Size: 997 368 42 731 226 152 2109 1697
Fragments arranged by size:
2109 1697 997 731 368 226 152 42
AseI AT'TA_AT
Cuts at: 160 1032 4876 160
Size: 872 3844 1606
Fragments arranged by size:
3844 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
AvaI C'yCGr_G
Cuts at: 1870 1943 2283 2385 2832 3431 3647 1870
Size: 73 340 102 447 599 216 4545
Fragments arranged by size:
4545 599 447 340 216 102 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2449 2482 2449
Size: 33 6289
BamHI G'GATC_C
Cuts at: 3652 4019 3652
Size: 367 5955
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2976 3497 3564 3643 5098
Size: 325 217 627 1189 521 67 79 1455
Cuts at: 5098 618
Size: 1842
Fragments arranged by size:
1842 1455 1189 627 521 325 217 79
67
BanII G_rGCy'C
Cuts at: 1391 2655 2763 3641 1391
Size: 1264 108 878 4072
Fragments arranged by size:
4072 1264 878 108
BbeI G_GCGC'C
Cuts at: 3568 3568
Size: 6322
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 928
Size: 971 199 5152
Fragments arranged by size:
5152 971 199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4513 4547 1903
Size: 34 2576 34 3678
Fragments arranged by size:
3678 2576 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2805 4214 5424 2805
Size: 1409 1210 3703
Fragments arranged by size:
3703 1409 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6322
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2708 4933 136
Size: 107 122 71 631 605 1036 2225 1525
Fragments arranged by size:
2225 1525 1036 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6317 6317
Size: 6322
BlpI GC'TnA_GC
Cuts at: 3438 3739 3438
Size: 301 6021
Bme1580I G_kGCm'C
Cuts at: 1790 3500 4383 5539 1790
Size: 1710 883 1156 2573
Fragments arranged by size:
2573 1710 1156 883
BmgBI CAC'GTC
Cuts at: 1318 2013 2104 1318
Size: 695 91 5536
Fragments arranged by size:
5536 695 91
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3121 3241 4053
Size: 416 53 83 399 24 1704 120 812
Cuts at: 4053 5011 442
Size: 958 1753
Fragments arranged by size:
1753 1704 958 812 416 399 120 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2531 2663 2750 4982 1239
Size: 1292 132 87 2232 2579
Fragments arranged by size:
2579 2232 1292 132 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3572 4447 5312 5610 3572
Size: 875 865 298 4284
Fragments arranged by size:
4284 875 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3348 4985 882
Size: 617 1849 1637 2219
Fragments arranged by size:
2219 1849 1637 617
BsaAI yAC'GTr
Cuts at: 493 1204 4113 493
Size: 711 2909 2702
Fragments arranged by size:
2909 2702 711
BsaBI GATnn'nnATC
Cuts at: 6316 6316
Size: 6322
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2130 2688 3565 4129
Size: 53 83 186 936 597 558 877 564
Cuts at: 4129 4511 5146 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 877 635 597 564 558 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2852 4755 5153 5427 5574 5985 2852
Size: 1903 398 274 147 411 3189
Fragments arranged by size:
3189 1903 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3125 3155 1178
Size: 30 165 30 1722 30 4345
Fragments arranged by size:
4345 1722 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2864 3182 3200 3203 3206 3215 3296
Size: 868 810 318 18 3 3 9 81
Cuts at: 3296 3299 3308 3391 3394 1186
Size: 3 9 83 3 4114
Fragments arranged by size:
4114 868 810 318 83 81 18 9
9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2430 3048 3076 3448 5525 6021 2430
Size: 618 28 372 2077 496 2731
Fragments arranged by size:
2731 2077 618 496 372 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2104 2315 3026 1163
Size: 573 368 211 711 4459
Fragments arranged by size:
4459 711 573 368 211
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2409 3772 4533 5561 1540
Size: 275 186 408 1363 761 1028 2301
Fragments arranged by size:
2301 1363 1028 761 408 275 186
BsiHKAI G_wGCw'C
Cuts at: 2394 2655 2763 4383 4468 5539 2394
Size: 261 108 1620 85 1071 3177
Fragments arranged by size:
3177 1620 1071 261 108 85
BsmI GAATG_Cn'
Cuts at: 3833 3926 3833
Size: 93 6229
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2394 2655 2763 3500 3641 4383 4468
Size: 399 604 261 108 737 141 742 85
Cuts at: 4468 5539 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 737 604 399 261 141
108 85
BspEI T'CCGG_A
Cuts at: 5985 5985
Size: 6322
BspHI T'CATG_A
Cuts at: 4211 5941 4211
Size: 1730 4592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2057 2079 2628 2988 3048 3677 844
Size: 1213 22 549 360 60 629 3489
Fragments arranged by size:
3489 1213 629 549 360 60 22
BsrBI CCG'CTC
Cuts at: 1394 1823 2242 2608 4209 1394
Size: 429 419 366 1601 3507
Fragments arranged by size:
3507 1601 429 419 366
BsrDI GCAATG_nn'
Cuts at: 65 1248 2826 4817 4991 65
Size: 1183 1578 1991 174 1396
Fragments arranged by size:
1991 1578 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3206 4966 5153 1700
Size: 110 1396 1760 187 2869
Fragments arranged by size:
2869 1760 1396 187 110
BsrGI T'GTAC_A
Cuts at: 96 3024 96
Size: 2928 3394
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6322
BssSI C'ACGA_G
Cuts at: 2773 4382 5394 2773
Size: 1609 1012 3701
Fragments arranged by size:
3701 1609 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6322
BstBI TT'CG_AA
Cuts at: 5126 5126
Size: 6322
BstEII G'GTnAC_C
Cuts at: 1184 2044 2278 1184
Size: 860 234 5228
Fragments arranged by size:
5228 860 234
BstXI CCAn_nnnn'nTGG
Cuts at: 2227 2227
Size: 6322
BstYI r'GATC_y
Cuts at: 1972 3652 4019 4404 4421 5873 5959 5971 6317
Size: 1680 367 385 17 1452 86 12 346
Cuts at: 6317 1972
Size: 1977
Fragments arranged by size:
1977 1680 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2471 2600 2894 2471
Size: 129 294 5899
Fragments arranged by size:
5899 294 129
BtgI C'CryG_G
Cuts at: 513 1065 2004 2798 513
Size: 552 939 794 4037
Fragments arranged by size:
4037 939 794 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3279 3460 487
Size: 683 56 2053 181 3349
Fragments arranged by size:
3349 2053 683 181 56
BtsI GCAGTG_nn'
Cuts at: 3452 3546 3838 4632 4652 3452
Size: 94 292 794 20 5122
Fragments arranged by size:
5122 794 292 94 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6322
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6287
DraI TTT'AAA
Cuts at: 3626 3626
Size: 6322
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5329 809
Size: 507 4013 1802
Fragments arranged by size:
4013 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2098 2217
Size: 54 1425 50 275 18 177 91 119
Cuts at: 2217 2406 2529 2967 3368 3593 3769 4658 6287
Size: 189 123 438 401 225 176 889 1629
Cuts at: 6287 8
Size: 43
Fragments arranged by size:
1629 1425 889 438 401 275 225 189
177 176 123 119 91 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2406 3769 1537
Size: 275 594 1363 4090
Fragments arranged by size:
4090 1363 594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 3150 3168 3177 3192 3264 3273 3285
Size: 678 1119 18 9 15 72 9 12
Cuts at: 3285 3338 4252 1353
Size: 53 914 3423
Fragments arranged by size:
3423 1119 914 678 72 53 18 15
12 9 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2123 4909 5283 5429 387
Size: 1064 138 534 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 534 374 146 138
EcoICRI GAG'CTC
Cuts at: 2653 2761 2653
Size: 108 6214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2531 2558 2663 2750 4385 4982
Size: 393 635 264 27 105 87 1635 597
Cuts at: 4982 5769 6037 1239
Size: 787 268 1524
Fragments arranged by size:
1635 1524 787 635 597 393 268 264
105 87 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2036 2036
Size: 6322
EcoO109I rG'GnC_Cy
Cuts at: 2077 2397 2077
Size: 320 6002
EcoRI G'AATT_C
Cuts at: 2858 2900 3631 4009 2858
Size: 42 731 378 5171
Fragments arranged by size:
5171 731 378 42
EcoRV GAT'ATC
Cuts at: 2524 2524
Size: 6322
FspI TGC'GCA
Cuts at: 4828 4828
Size: 6322
HaeII r_GCGC'y
Cuts at: 1678 3392 3568 5469 1678
Size: 1714 176 1901 2531
Fragments arranged by size:
2531 1901 1714 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2948 2980 3125 3157 3239
Size: 32 135 32 1376 32 145 32 82
Cuts at: 3239 3271 3459 3491 4963 4995 5037 5069 1373
Size: 32 188 32 1472 32 42 32 2626
Fragments arranged by size:
2626 1472 1376 188 145 135 82 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3666 3912 669
Size: 988 281 1728 246 3079
Fragments arranged by size:
3079 1728 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6322
HpaI GTT'AAC
Cuts at: 3912 3912
Size: 6322
KasI G'GCGC_C
Cuts at: 3564 3564
Size: 6322
KpnI G_GTAC'C
Cuts at: 2980 3647 2980
Size: 667 5655
MfeI C'AATT_G
Cuts at: 3921 3921
Size: 6322
MluI A'CGCG_T
Cuts at: 6198 6198
Size: 6322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2224 5436 5620 1305
Size: 919 3212 184 2007
Fragments arranged by size:
3212 2007 919 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2100 2219 3595 10
Size: 54 1425 343 177 91 119 1376 2737
Fragments arranged by size:
2737 1425 1376 343 177 119 91 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4280 4639 4798 518
Size: 648 3114 359 159 2042
Fragments arranged by size:
3114 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2632 2753 2986 3738 4415 5563 5808
Size: 512 514 121 233 752 677 1148 245
Cuts at: 5808 1606
Size: 2120
Fragments arranged by size:
2120 1148 752 677 514 512 245 233
121
NaeI GCC'GGC
Cuts at: 3208 3208
Size: 6322
NarI GG'CG_CC
Cuts at: 3565 3565
Size: 6322
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4831
Fragments arranged by size:
4831 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6322
NgoMIV G'CCGG_C
Cuts at: 3206 3206
Size: 6322
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6322
NotI GC'GGCC_GC
Cuts at: 3769 3769
Size: 6322
NspI r_CATG'y
Cuts at: 1132 1229 3680 5225 1132
Size: 97 2451 1545 2229
Fragments arranged by size:
2451 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6322
PciI A'CATG_T
Cuts at: 5221 5221
Size: 6322
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3377 1108
Size: 2269 4053
PfoI T'CCnGG_A
Cuts at: 2547 2547
Size: 6322
PmeI GTTT'AAAC
Cuts at: 3626 3626
Size: 6322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4391 4423 5904 5936 4391
Size: 32 1481 32 4777
Fragments arranged by size:
4777 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2077 2077
Size: 6322
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6322
PsiI TTA'TAA
Cuts at: 3892 3892
Size: 6322
PspXI vC'TCGA_Gb
Cuts at: 2832 2832
Size: 6322
PstI C_TGCA'G
Cuts at: 830 2758 2985 3674 830
Size: 1928 227 689 3478
Fragments arranged by size:
3478 1928 689 227
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6322
PvuII CAG'CTG
Cuts at: 2118 2632 2753 2986 2118
Size: 514 121 233 5454
Fragments arranged by size:
5454 514 233 121
SacI G_AGCT'C
Cuts at: 2655 2763 2655
Size: 108 6214
SalI G'TCGA_C
Cuts at: 1936 3664 1936
Size: 1728 4594
SanDI GG'GwC_CC
Cuts at: 2077 2077
Size: 6322
SbfI CC_TGCA'GG
Cuts at: 3674 3674
Size: 6322
ScaI AGT'ACT
Cuts at: 4570 6282 4570
Size: 1712 4610
SfcI C'TryA_G
Cuts at: 826 1045 2754 2981 3670 4805 5486 5677 826
Size: 219 1709 227 689 1135 681 191 1471
Fragments arranged by size:
1709 1471 1135 689 681 227 219 191
SfoI GGC'GCC
Cuts at: 3566 3566
Size: 6322
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6322
SmaI CCC'GGG
Cuts at: 1872 2285 2387 3649 1872
Size: 413 102 1262 4545
Fragments arranged by size:
4545 1262 413 102
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2832 3587 4426 5327 5589 820
Size: 197 926 889 755 839 901 262 1553
Fragments arranged by size:
1553 926 901 889 839 755 262 197
SnaBI TAC'GTA
Cuts at: 493 4113 493
Size: 3620 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6322
SphI G_CATG'C
Cuts at: 1132 3680 1132
Size: 2548 3774
SspI AAT'ATT
Cuts at: 5 52 4246 5
Size: 47 4194 2081
Fragments arranged by size:
4194 2081 47
StuI AGG'CCT
Cuts at: 1517 2892 1517
Size: 1375 4947
StyI C'CwwG_G
Cuts at: 513 1065 2004 3761 513
Size: 552 939 1757 3074
Fragments arranged by size:
3074 1757 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4349 4366 4519 4704 5149 325
Size: 1255 2769 17 153 185 445 1498
Fragments arranged by size:
2769 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2562 3024 4568
Size: 274 80 33 51 1443 585 462 1544
Cuts at: 4568 6280 96
Size: 1712 138
Fragments arranged by size:
1712 1544 1443 585 462 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2987 3019 3368 3400 6282 6314 2987
Size: 32 349 32 2882 32 2995
Fragments arranged by size:
2995 2882 349 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6322
XbaI T'CTAG_A
Cuts at: 3658 3658
Size: 6322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2833 2833
Size: 6322
XhoI C'TCGA_G
Cuts at: 1943 2832 1943
Size: 889 5433
XmaI C'CCGG_G
Cuts at: 1870 2283 2385 3647 1870
Size: 413 102 1262 4545
Fragments arranged by size:
4545 1262 413 102
XmnI GAAnn'nnTTC
Cuts at: 2807 4451 2807
Size: 1644 4678
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2131 4130 276
Size: 53 83 186 936 597 1999 2468
Fragments arranged by size:
2468 1999 936 597 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII FalI FseI FspAI
NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII
SapI SexAI SfiI SrfI SwaI