Restriction map for
pFN21AE4133
(Circular) (Six-base) MAPSORT of: pFN21AE4133.seq Check: 8511 from: 1 to: 6319
pFN21AE4133 6319 bp
With 182 enzymes: SgfI *
March 28, 2012 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2452 2452
Size: 6319
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3662 1937
Size: 1725 4594
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1996 2253 3179 3474 4382 5766 6034 1632
Size: 364 257 926 295 908 1384 268 1917
Fragments arranged by size:
1917 1384 926 908 364 295 268 257
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 5218 6195 1203
Size: 4015 977 1327
Fragments arranged by size:
4015 1327 977
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2474 1184
Size: 1290 5029
AlwNI CAG_nnn'CTG
Cuts at: 2061 2150 2188 2813 4062 5634 2061
Size: 89 38 625 1249 1572 2746
Fragments arranged by size:
2746 1572 1249 625 89 38
ApaI G_GGCC'C
Cuts at: 3352 3352
Size: 6319
ApaLI G'TGCA_C
Cuts at: 2560 4376 5532 2560
Size: 1816 1156 3347
Fragments arranged by size:
3347 1816 1156
ApoI r'AATT_y
Cuts at: 1493 3628 3854 4006 6115 1493
Size: 2135 226 152 2109 1697
Fragments arranged by size:
2135 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 2396 3644 1870
Size: 73 453 1248 4545
Fragments arranged by size:
4545 1248 453 73
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3401 3640 5095 618
Size: 325 217 627 1614 239 1455 1842
Fragments arranged by size:
1842 1614 1455 627 325 239 217
BanII G_rGCy'C
Cuts at: 1391 2177 3352 3369 3638 1391
Size: 786 1175 17 269 4072
Fragments arranged by size:
4072 1175 786 269 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5348
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4510 4544 1903
Size: 34 2573 34 3678
Fragments arranged by size:
3678 2573 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4211 5421 4211
Size: 1210 5109
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2411 3098 4930
Size: 107 122 71 631 605 739 687 1832
Cuts at: 4930 136
Size: 1525
Fragments arranged by size:
1832 1525 739 687 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6314 6314
Size: 6319
BlpI GC'TnA_GC
Cuts at: 3736 3736
Size: 6319
Bme1580I G_kGCm'C
Cuts at: 1790 2353 2564 3352 4380 5536 1790
Size: 563 211 788 1028 1156 2573
Fragments arranged by size:
2573 1156 1028 788 563 211
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2034 2832 4050
Size: 416 53 83 399 24 617 798 1218
Cuts at: 4050 5008 442
Size: 958 1753
Fragments arranged by size:
1753 1218 958 798 617 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2856 2856
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 2878 4979 1239
Size: 1028 611 2101 2579
Fragments arranged by size:
2579 2101 1028 611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2369 3020 4444 5309 5607 2369
Size: 651 1424 865 298 3081
Fragments arranged by size:
3081 1424 865 651 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4982 882
Size: 617 3483 2219
Fragments arranged by size:
3483 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2617 4110 493
Size: 711 1413 1493 2702
Fragments arranged by size:
2702 1493 1413 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4126 4508 5143 275
Size: 53 83 186 936 2593 382 635 1451
Fragments arranged by size:
2593 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4752 5150 5424 5571 5982 4752
Size: 398 274 147 411 5089
Fragments arranged by size:
5089 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 3081 3111 3119
Size: 30 165 30 687 30 961 30 8
Cuts at: 3119 3149 1178
Size: 30 4348
Fragments arranged by size:
4348 961 687 165 30 30 30 30
30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2115 2384 2447 2489 3185 1186
Size: 929 269 63 42 696 4320
Fragments arranged by size:
4320 929 696 269 63 42
BseYI C'CCAG_C
Cuts at: 2129 2488 2527 3351 3459 5522 6018 2129
Size: 359 39 824 108 2063 496 2430
Fragments arranged by size:
2430 2063 824 496 359 108 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2480 2652 1163
Size: 573 744 172 4830
Fragments arranged by size:
4830 744 573 172
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3769 4530 5558 1540
Size: 275 186 1768 761 1028 2301
Fragments arranged by size:
2301 1768 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2177 2564 2822 3369 4380 4465 5536 2177
Size: 387 258 547 1011 85 1071 2960
Fragments arranged by size:
2960 1071 1011 547 387 258 85
BsmI GAATG_Cn'
Cuts at: 3532 3830 3923 3532
Size: 298 93 5928
Fragments arranged by size:
5928 298 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2285 2285
Size: 6319
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2353 2564 2822 3352 3369 3638
Size: 399 387 176 211 258 530 17 269
Cuts at: 3638 4380 4465 5536 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 530 399 387 269 258
211 176 85 17
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 3225 4208 5938 3225
Size: 983 1730 3606
Fragments arranged by size:
3606 1730 983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2452 3575 3674 844
Size: 1608 1123 99 3489
Fragments arranged by size:
3489 1608 1123 99
BsrBI CCG'CTC
Cuts at: 1394 1823 4206 1394
Size: 429 2383 3507
Fragments arranged by size:
3507 2383 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4814 4988 65
Size: 1183 3566 174 1396
Fragments arranged by size:
3566 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4963 5150 1700
Size: 110 3153 187 2869
Fragments arranged by size:
3153 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6319
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2188 794
Size: 1394 4925
BstBI TT'CG_AA
Cuts at: 3184 5123 3184
Size: 1939 4380
BstEII G'GTnAC_C
Cuts at: 1184 2297 3049 1184
Size: 1113 752 4454
Fragments arranged by size:
4454 1113 752
BstXI CCAn_nnnn'nTGG
Cuts at: 2689 3321 2689
Size: 632 5687
BstYI r'GATC_y
Cuts at: 1972 2908 3649 4016 4401 4418 5870 5956 5968
Size: 936 741 367 385 17 1452 86 12
Cuts at: 5968 6314 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 936 741 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2303 2367 2757 3243 2303
Size: 64 390 486 5379
Fragments arranged by size:
5379 486 390 64
BtgI C'CryG_G
Cuts at: 513 1065 2424 513
Size: 552 1359 4408
Fragments arranged by size:
4408 1359 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 2226 3835 4629 4649 2226
Size: 1609 794 20 3896
Fragments arranged by size:
3896 1609 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3239 3274 529
Size: 35 2675 35 3574
Fragments arranged by size:
3574 2675 35 35
DraI TTT'AAA
Cuts at: 3151 3623 3151
Size: 472 5847
DraIII CAC_nnn'GTG
Cuts at: 2938 2938
Size: 6319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3766 4655 6284
Size: 54 1425 50 275 18 1936 889 1629
Cuts at: 6284 8
Size: 43
Fragments arranged by size:
1936 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 2424 2796 3288 3495 4249 1353
Size: 680 391 372 492 207 754 3423
Fragments arranged by size:
3423 754 680 492 391 372 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4906 5280 5426 387
Size: 1064 138 3317 374 146 1280
Fragments arranged by size:
3317 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2175 3367 2175
Size: 1192 5127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1996 2253 2267 2878 3179 3474 4382
Size: 393 364 257 14 611 301 295 908
Cuts at: 4382 4979 5766 6034 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 908 787 611 597 393 364 301
295 268 257 14
EcoO109I rG'GnC_Cy
Cuts at: 2507 2810 2507
Size: 303 6016
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
EcoRV GAT'ATC
Cuts at: 3197 3197
Size: 6319
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 3358 5466 1678
Size: 1680 2108 2531
Fragments arranged by size:
2531 2108 1680
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2634 2666 3207 3239 3374
Size: 32 135 32 1062 32 541 32 135
Cuts at: 3374 3406 3569 3601 4960 4992 5034 5066 1373
Size: 32 163 32 1359 32 42 32 2626
Fragments arranged by size:
2626 1359 1062 541 163 135 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3663 3909 669
Size: 988 281 1725 246 3079
Fragments arranged by size:
3079 1725 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5433 5617 1305
Size: 4128 184 2007
Fragments arranged by size:
4128 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4497
Fragments arranged by size:
4497 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 3381 4277 4636 4795 518
Size: 648 978 1237 896 359 159 2042
Fragments arranged by size:
2042 1237 978 896 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2206 2454 2488 2701 2777 3735 4412 5560
Size: 600 248 34 213 76 958 677 1148
Cuts at: 5560 5805 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 958 677 600 248 245 213
76 34
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5767
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 2195 2548 2767 3677 5222 1132
Size: 97 966 353 219 910 1545 2229
Fragments arranged by size:
2229 1545 966 910 353 219 97
PasI CC'CwG_GG
Cuts at: 1348 2110 2555 2813 1348
Size: 762 445 258 4854
Fragments arranged by size:
4854 762 445 258
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2729 1108
Size: 1621 4698
PfoI T'CCnGG_A
Cuts at: 2283 2283
Size: 6319
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3252 1613
Size: 1639 4680
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PspOMI G'GGCC_C
Cuts at: 3348 3348
Size: 6319
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2628 2660 2628
Size: 32 6287
PstI C_TGCA'G
Cuts at: 830 3671 830
Size: 2841 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
PvuII CAG'CTG
Cuts at: 2488 2488
Size: 6319
SacI G_AGCT'C
Cuts at: 2177 3369 2177
Size: 1192 5127
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SfcI C'TryA_G
Cuts at: 826 1045 3667 4802 5483 5674 826
Size: 219 2622 1135 681 191 1471
Fragments arranged by size:
2622 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 2398 3646 1872
Size: 526 1248 4545
Fragments arranged by size:
4545 1248 526
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2384 3035 4423 5324 5586 820
Size: 197 926 441 651 1388 901 262 1553
Fragments arranged by size:
1553 1388 926 901 651 441 262 197
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3677 1132
Size: 2545 3774
SspI AAT'ATT
Cuts at: 5 52 3004 4243 5
Size: 47 2952 1239 2081
Fragments arranged by size:
2952 2081 1239 47
StuI AGG'CCT
Cuts at: 1517 3156 1517
Size: 1639 4680
StyI C'CwwG_G
Cuts at: 513 1065 2796 3758 513
Size: 552 1731 962 3074
Fragments arranged by size:
3074 1731 962 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2285 3313 4346 4363 4516 4701 5146
Size: 1255 705 1028 1033 17 153 185 445
Cuts at: 5146 325
Size: 1498
Fragments arranged by size:
1498 1255 1033 1028 705 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2635 2914 4565
Size: 274 80 33 51 1443 658 279 1651
Cuts at: 4565 6277 96
Size: 1712 138
Fragments arranged by size:
1712 1651 1443 658 279 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2420 2452 3081 3113 6279 6311 2420
Size: 32 629 32 3166 32 2428
Fragments arranged by size:
3166 2428 629 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 2396 3644 1870
Size: 526 1248 4545
Fragments arranged by size:
4545 1248 526
XmnI GAAnn'nnTTC
Cuts at: 3587 4448 3587
Size: 861 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI