Restriction map for
pFN21AE4131
(Circular) (Six-base) MAPSORT of: pFN21AE4131.seq Check: 5927 from: 1 to: 6130
pFN21AE4131 6130 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2160 3473 1937
Size: 223 1313 4594
Fragments arranged by size:
4594 1313 223
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2660 3127 3378 4193 5577 5845 1632
Size: 1028 467 251 815 1384 268 1917
Fragments arranged by size:
1917 1384 1028 815 467 268 251
AfeI AGC'GCT
Cuts at: 2145 2145
Size: 6130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 3097 5029 6006 1203
Size: 1894 1932 977 1327
Fragments arranged by size:
1932 1894 1327 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2108 3435 1184
Size: 924 1327 3879
Fragments arranged by size:
3879 1327 924
AlwNI CAG_nnn'CTG
Cuts at: 2095 3873 5445 2095
Size: 1778 1572 2780
Fragments arranged by size:
2780 1778 1572
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 3124 3439 3665 3817 5926 1493
Size: 1631 315 226 152 2109 1697
Fragments arranged by size:
2109 1697 1631 315 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 3455 1870
Size: 73 1512 4545
Fragments arranged by size:
4545 1512 73
BamHI G'GATC_C
Cuts at: 3460 3827 3460
Size: 367 5763
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3451 4906 618
Size: 325 217 627 1664 1455 1842
Fragments arranged by size:
1842 1664 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 3449 1391
Size: 621 1437 4072
Fragments arranged by size:
4072 1437 621
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2329 928
Size: 971 430 4729
Fragments arranged by size:
4729 971 430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 1903
Size: 34 2384 34 3678
Fragments arranged by size:
3678 2384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 2902 1548
Size: 1354 4776
BfrBI ATG'CAT
Cuts at: 2877 2877
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4741 136
Size: 107 122 71 631 605 3069 1525
Fragments arranged by size:
3069 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3322 6125 3322
Size: 2803 3327
BlpI GC'TnA_GC
Cuts at: 3547 3547
Size: 6130
Bme1580I G_kGCm'C
Cuts at: 1790 3043 3241 4191 5347 1790
Size: 1253 198 950 1156 2573
Fragments arranged by size:
2573 1253 1156 950 198
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3861 4819 442
Size: 416 53 83 399 24 2444 958 1753
Fragments arranged by size:
2444 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2059 2117 3224 4790 1239
Size: 820 58 1107 1566 2579
Fragments arranged by size:
2579 1566 1107 820 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2214 4255 5120 5418 2214
Size: 2041 865 298 2926
Fragments arranged by size:
2926 2041 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4793 882
Size: 617 3294 2219
Fragments arranged by size:
3294 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3921 493
Size: 711 2717 2702
Fragments arranged by size:
2717 2702 711
BsaBI GATnn'nnATC
Cuts at: 2181 6124 2181
Size: 3943 2187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3937 4319 4954 275
Size: 53 83 186 936 2404 382 635 1451
Fragments arranged by size:
2404 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2438 2922 4563 4961 5235 5382 5793 2438
Size: 484 1641 398 274 147 411 2775
Fragments arranged by size:
2775 1641 484 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5905
Fragments arranged by size:
5905 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2425 1186
Size: 1239 4891
BseYI C'CCAG_C
Cuts at: 2348 2455 3003 5333 5829 2348
Size: 107 548 2330 496 2649
Fragments arranged by size:
2649 2330 548 496 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5557
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4341 5369 1540
Size: 275 186 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 4191 4276 5347 2012
Size: 2179 85 1071 2795
Fragments arranged by size:
2795 2179 1071 85
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 3043 3241 3449 4191 4276 5347
Size: 399 222 1031 198 208 742 85 1071
Cuts at: 5347 1391
Size: 2174
Fragments arranged by size:
2174 1071 1031 742 399 222 208 198
85
BspEI T'CCGG_A
Cuts at: 2438 5793 2438
Size: 3355 2775
BspHI T'CATG_A
Cuts at: 2488 4019 5749 2488
Size: 1531 1730 2869
Fragments arranged by size:
2869 1730 1531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3485 844
Size: 2641 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4017 1394
Size: 429 2194 3507
Fragments arranged by size:
3507 2194 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4625 4799 65
Size: 1183 3377 174 1396
Fragments arranged by size:
3377 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4774 4961 1700
Size: 110 2964 187 2869
Fragments arranged by size:
2964 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6130
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 4190 5202 4190
Size: 1012 5118
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6130
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstYI r'GATC_y
Cuts at: 1972 2127 2515 3322 3460 3827 4212 4229 5681
Size: 155 388 807 138 367 385 17 1452
Cuts at: 5681 5767 5779 6125 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 807 388 385 367 346 155
138 86 17 12
BstZ17I GTA'TAC
Cuts at: 2161 2161
Size: 6130
Bsu36I CC'TnA_GG
Cuts at: 2342 2342
Size: 6130
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5578
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2854 487
Size: 683 56 1628 3763
Fragments arranged by size:
3763 1628 683 56
BtsI GCAGTG_nn'
Cuts at: 3646 4440 4460 3646
Size: 794 20 5316
Fragments arranged by size:
5316 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6095
DraI TTT'AAA
Cuts at: 2629 2718 3434 2629
Size: 89 716 5325
Fragments arranged by size:
5325 716 89
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5137 809
Size: 507 3821 1802
Fragments arranged by size:
3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2962 3577 4466
Size: 54 1425 50 275 18 1132 615 889
Cuts at: 4466 6095 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1132 889 615 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3060 4060 1353
Size: 1707 1000 3423
Fragments arranged by size:
3423 1707 1000
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4717 5091 5237 387
Size: 1064 138 3128 374 146 1280
Fragments arranged by size:
3128 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 6130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2117 2660 3127 3224 3378 4193
Size: 393 427 58 543 467 97 154 815
Cuts at: 4193 4790 5577 5845 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 815 787 597 543 467 427 393
268 154 97 58
EcoO109I rG'GnC_Cy
Cuts at: 2285 2285
Size: 6130
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
FspI TGC'GCA
Cuts at: 3348 4636 3348
Size: 1288 4842
HaeII r_GCGC'y
Cuts at: 1678 2147 5277 1678
Size: 469 3130 2531
Fragments arranged by size:
3130 2531 469
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3307 3339 4771 4803 4845
Size: 32 135 32 1735 32 1432 32 42
Cuts at: 4845 4877 1373
Size: 32 2626
Fragments arranged by size:
2626 1735 1432 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3160 3474 3720 669
Size: 988 281 1222 314 246 3079
Fragments arranged by size:
3079 1222 988 314 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6130
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5244 5428 1305
Size: 3939 184 2007
Fragments arranged by size:
3939 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4308
Fragments arranged by size:
4308 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2493 3276 4088 4447 4606 518
Size: 648 924 403 783 812 359 159 2042
Fragments arranged by size:
2042 924 812 783 648 403 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 3074 3546 4223 5371 5616 1606
Size: 420 1048 472 677 1148 245 2120
Fragments arranged by size:
2120 1148 1048 677 472 420 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5578
NdeI CA'TA_TG
Cuts at: 387 3273 387
Size: 2886 3244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NsiI A_TGCA'T
Cuts at: 2879 2879
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 2877 3101 3488 5033 1132
Size: 97 1648 224 387 1545 2229
Fragments arranged by size:
2229 1648 1545 387 224 97
PacI TTA_AT'TAA
Cuts at: 3399 3399
Size: 6130
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 3097 5029 3097
Size: 1932 4198
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2243 1108
Size: 1135 4995
PfoI T'CCnGG_A
Cuts at: 2749 2749
Size: 6130
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 2958 3700 2958
Size: 742 5388
PstI C_TGCA'G
Cuts at: 830 2946 3330 3482 830
Size: 2116 384 152 3478
Fragments arranged by size:
3478 2116 384 152
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
PvuII CAG'CTG
Cuts at: 3074 3074
Size: 6130
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 6130
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SfcI C'TryA_G
Cuts at: 826 1045 2223 2590 2942 3326 3478 4613 5294
Size: 219 1178 367 352 384 152 1135 681
Cuts at: 5294 5485 826
Size: 191 1471
Fragments arranged by size:
1471 1178 1135 681 384 367 352 219
191 152
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 3457 1872
Size: 1585 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2229 4234 5135 5397 820
Size: 197 926 286 2005 901 262 1553
Fragments arranged by size:
2005 1553 926 901 286 262 197
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 2877 3488 1132
Size: 1745 611 3774
Fragments arranged by size:
3774 1745 611
SspI AAT'ATT
Cuts at: 5 52 2825 4054 5
Size: 47 2773 1229 2081
Fragments arranged by size:
2773 2081 1229 47
StuI AGG'CCT
Cuts at: 1517 3182 1517
Size: 1665 4465
StyI C'CwwG_G
Cuts at: 513 1065 3569 513
Size: 552 2504 3074
Fragments arranged by size:
3074 2504 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2413 4157 4174 4327 4512 4957 325
Size: 1255 833 1744 17 153 185 445 1498
Fragments arranged by size:
1744 1498 1255 833 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2547 2569 2670
Size: 274 80 33 51 1443 570 22 101
Cuts at: 2670 4376 6088 96
Size: 1706 1712 138
Fragments arranged by size:
1712 1706 1443 570 274 138 101 80
51 33 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2546 2578 6090 6122 2546
Size: 32 3512 32 2554
Fragments arranged by size:
3512 2554 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6130
XbaI T'CTAG_A
Cuts at: 3466 3466
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 3455 1870
Size: 1585 4545
XmnI GAAnn'nnTTC
Cuts at: 4259 4259
Size: 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI