Restriction map for
pFN21AE4129
(Circular) (Six-base) MAPSORT of: pFN21AE4129.seq Check: 6252 from: 1 to: 6283
pFN21AE4129 6283 bp
With 182 enzymes: SgfI *
March 28, 2012 13:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4093 278
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2280 3626 1937
Size: 343 1346 4594
Fragments arranged by size:
4594 1346 343
Acc65I G'GTAC_C
Cuts at: 3604 3604
Size: 6283
AclI AA'CG_TT
Cuts at: 1563 4410 4783 1563
Size: 2847 373 3063
Fragments arranged by size:
3063 2847 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2177 3184 3528 3539 4346 5730 5998
Size: 464 81 1007 344 11 807 1384 268
Cuts at: 5998 1632
Size: 1917
Fragments arranged by size:
1917 1384 1007 807 464 344 268 81
11
AflII C'TTAA_G
Cuts at: 820 1017 2843 820
Size: 197 1826 4260
Fragments arranged by size:
4260 1826 197
AflIII A'CryG_T
Cuts at: 1203 5182 6159 1203
Size: 3979 977 1327
Fragments arranged by size:
3979 1327 977
AgeI A'CCGG_T
Cuts at: 5114 5114
Size: 6283
AhdI GACnn_n'nnGTC
Cuts at: 5012 5012
Size: 6283
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6283
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2340 2372 2340
Size: 32 6251
AlwNI CAG_nnn'CTG
Cuts at: 4026 5598 4026
Size: 1572 4711
ApaLI G'TGCA_C
Cuts at: 2341 4340 5496 2341
Size: 1999 1156 3128
Fragments arranged by size:
3128 1999 1156
ApoI r'AATT_y
Cuts at: 1493 2159 2316 3429 3592 3818 3970 6079 1493
Size: 666 157 1113 163 226 152 2109 1697
Fragments arranged by size:
2109 1697 1113 666 226 163 157 152
AseI AT'TA_AT
Cuts at: 160 1032 2489 2493 4837 160
Size: 872 1457 4 2344 1606
Fragments arranged by size:
2344 1606 1457 872 4
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
AvaI C'yCGr_G
Cuts at: 1870 1943 2976 3608 1870
Size: 73 1033 632 4545
Fragments arranged by size:
4545 1033 632 73
AvrII C'CTAG_G
Cuts at: 3409 3409
Size: 6283
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2973 3006 2973
Size: 33 6250
BamHI G'GATC_C
Cuts at: 3613 3980 3613
Size: 367 5916
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3604 5059 618
Size: 325 217 627 1817 1455 1842
Fragments arranged by size:
1842 1817 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2069 2791 3319 3602 1391
Size: 678 722 528 283 4072
Fragments arranged by size:
4072 722 678 528 283
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2102 928
Size: 971 203 5109
Fragments arranged by size:
5109 971 203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4474 4508 1903
Size: 34 2537 34 3678
Fragments arranged by size:
3678 2537 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2366 2974 3359 3407 4175 5385 2366
Size: 608 385 48 768 1210 3264
Fragments arranged by size:
3264 1210 768 608 385 48
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6283
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4894 136
Size: 107 122 71 631 605 3222 1525
Fragments arranged by size:
3222 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6278 6278
Size: 6283
BlpI GC'TnA_GC
Cuts at: 3700 3700
Size: 6283
Bme1580I G_kGCm'C
Cuts at: 1790 2345 4344 5500 1790
Size: 555 1999 1156 2573
Fragments arranged by size:
2573 1999 1156 555
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6283
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2645 4014 4972
Size: 416 53 83 399 24 1228 1369 958
Cuts at: 4972 442
Size: 1753
Fragments arranged by size:
1753 1369 1228 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4943 1239
Size: 3704 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2045 2501 4408 5273 5571 2045
Size: 456 1907 865 298 2757
Fragments arranged by size:
2757 1907 865 456 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2110 4946 882
Size: 617 611 2836 2219
Fragments arranged by size:
2836 2219 617 611
BsaAI yAC'GTr
Cuts at: 493 1204 2693 4074 493
Size: 711 1489 1381 2702
Fragments arranged by size:
2702 1489 1381 711
BsaBI GATnn'nnATC
Cuts at: 6277 6277
Size: 6283
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4090 4472 5107 275
Size: 53 83 186 936 2557 382 635 1451
Fragments arranged by size:
2557 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4716 5114 5388 5535 5946 4716
Size: 398 274 147 411 5053
Fragments arranged by size:
5053 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6058
Fragments arranged by size:
6058 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 1186
Size: 844 5439
BseYI C'CCAG_C
Cuts at: 5486 5982 5486
Size: 496 5787
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2232 1163
Size: 573 496 5214
Fragments arranged by size:
5214 573 496
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3733 4494 5522 1540
Size: 275 186 1732 761 1028 2301
Fragments arranged by size:
2301 1732 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2345 4344 4429 5500 2345
Size: 1999 85 1071 3128
Fragments arranged by size:
3128 1999 1071 85
BsmI GAATG_Cn'
Cuts at: 2203 2606 3005 3794 3887 2203
Size: 403 399 789 93 4599
Fragments arranged by size:
4599 789 403 399 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2345 2791 3319 3602 4344 4429
Size: 399 279 276 446 528 283 742 85
Cuts at: 4429 5500 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 528 446 399 283 279
276 85
BspEI T'CCGG_A
Cuts at: 5946 5946
Size: 6283
BspHI T'CATG_A
Cuts at: 4172 5902 4172
Size: 1730 4553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3638 844
Size: 2794 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4170 1394
Size: 429 2347 3507
Fragments arranged by size:
3507 2347 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2923 4778 4952 65
Size: 1183 1675 1855 174 1396
Fragments arranged by size:
1855 1675 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4927 5114 1700
Size: 110 3117 187 2869
Fragments arranged by size:
3117 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6283
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6283
BssSI C'ACGA_G
Cuts at: 4343 5355 4343
Size: 1012 5271
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3518 794
Size: 2724 3559
BstBI TT'CG_AA
Cuts at: 5087 5087
Size: 6283
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6283
BstYI r'GATC_y
Cuts at: 1972 3613 3980 4365 4382 5834 5920 5932 6278
Size: 1641 367 385 17 1452 86 12 346
Cuts at: 6278 1972
Size: 1977
Fragments arranged by size:
1977 1641 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2281 2281
Size: 6283
Bsu36I CC'TnA_GG
Cuts at: 2469 2817 2469
Size: 348 5935
BtgI C'CryG_G
Cuts at: 513 1065 2763 513
Size: 552 1698 4033
Fragments arranged by size:
4033 1698 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5544
Fragments arranged by size:
5544 683 56
BtsI GCAGTG_nn'
Cuts at: 3477 3799 4593 4613 3477
Size: 322 794 20 5147
Fragments arranged by size:
5147 794 322 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6248
DraI TTT'AAA
Cuts at: 2167 2331 3537 3587 2167
Size: 164 1206 50 4863
Fragments arranged by size:
4863 1206 164 50
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2757 5290 809
Size: 507 1441 2533 1802
Fragments arranged by size:
2533 1802 1441 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3730 4619 6248
Size: 54 1425 50 275 18 1900 889 1629
Cuts at: 6248 8
Size: 43
Fragments arranged by size:
1900 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3730 1537
Size: 275 1918 4090
Fragments arranged by size:
4090 1918 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2318 3223 3270 3555 4213 1353
Size: 965 905 47 285 658 3423
Fragments arranged by size:
3423 965 905 658 285 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4870 5244 5390 387
Size: 1064 138 3281 374 146 1280
Fragments arranged by size:
3281 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2177 3184 3528 3539 4346 4943
Size: 393 464 81 1007 344 11 807 597
Cuts at: 4943 5730 5998 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1007 807 787 597 464 393 344
268 81 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3482 3482
Size: 6283
EcoRI G'AATT_C
Cuts at: 2159 3592 3970 2159
Size: 1433 378 4472
Fragments arranged by size:
4472 1433 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2417 3533 2179
Size: 238 1116 4929
Fragments arranged by size:
4929 1116 238
FspI TGC'GCA
Cuts at: 4789 4789
Size: 6283
HaeII r_GCGC'y
Cuts at: 1678 5430 1678
Size: 3752 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3476 3508 4924 4956 4998
Size: 32 135 32 1904 32 1416 32 42
Cuts at: 4998 5030 1373
Size: 32 2626
Fragments arranged by size:
2626 1904 1416 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2907 3627 3873 669
Size: 988 281 969 720 246 3079
Fragments arranged by size:
3079 988 969 720 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6283
HpaI GTT'AAC
Cuts at: 3873 3873
Size: 6283
KpnI G_GTAC'C
Cuts at: 3608 3608
Size: 6283
MfeI C'AATT_G
Cuts at: 3882 3882
Size: 6283
MluI A'CGCG_T
Cuts at: 6159 6159
Size: 6283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2001 5397 5581 1305
Size: 696 3396 184 2007
Fragments arranged by size:
3396 2007 696 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4461
Fragments arranged by size:
4461 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3340 4241 4600 4759 518
Size: 648 2174 901 359 159 2042
Fragments arranged by size:
2174 2042 901 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2086 3449 3506 3699 4376 5524 5769 1606
Size: 480 1363 57 193 677 1148 245 2120
Fragments arranged by size:
2120 1363 1148 677 480 245 193 57
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5731
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6283
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6283
NotI GC'GGCC_GC
Cuts at: 3730 3730
Size: 6283
NspI r_CATG'y
Cuts at: 1132 1229 2690 3641 5186 1132
Size: 97 1461 951 1545 2229
Fragments arranged by size:
2229 1545 1461 951 97
PacI TTA_AT'TAA
Cuts at: 2493 2493
Size: 6283
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6283
PciI A'CATG_T
Cuts at: 5182 5182
Size: 6283
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3086 1108
Size: 1978 4305
PmeI GTTT'AAAC
Cuts at: 3587 3587
Size: 6283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4352 4384 5865 5897 4352
Size: 32 1481 32 4738
Fragments arranged by size:
4738 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6283
PsiI TTA'TAA
Cuts at: 3853 3853
Size: 6283
PstI C_TGCA'G
Cuts at: 830 3505 3635 830
Size: 2675 130 3478
Fragments arranged by size:
3478 2675 130
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6283
PvuII CAG'CTG
Cuts at: 2086 3449 3506 2086
Size: 1363 57 4863
Fragments arranged by size:
4863 1363 57
SalI G'TCGA_C
Cuts at: 1936 3625 1936
Size: 1689 4594
SbfI CC_TGCA'GG
Cuts at: 3635 3635
Size: 6283
ScaI AGT'ACT
Cuts at: 4531 6243 4531
Size: 1712 4571
SfcI C'TryA_G
Cuts at: 826 1045 3375 3501 3631 4766 5447 5638 826
Size: 219 2330 126 130 1135 681 191 1471
Fragments arranged by size:
2330 1471 1135 681 219 191 130 126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6283
SmaI CCC'GGG
Cuts at: 1872 2978 3610 1872
Size: 1106 632 4545
Fragments arranged by size:
4545 1106 632
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2060 2480 2843 4387 5288 5550
Size: 197 926 117 420 363 1544 901 262
Cuts at: 5550 820
Size: 1553
Fragments arranged by size:
1553 1544 926 901 420 363 262 197
117
SnaBI TAC'GTA
Cuts at: 493 2693 4074 493
Size: 2200 1381 2702
Fragments arranged by size:
2702 2200 1381
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6283
SphI G_CATG'C
Cuts at: 1132 3641 1132
Size: 2509 3774
SspI AAT'ATT
Cuts at: 5 52 2803 4207 5
Size: 47 2751 1404 2081
Fragments arranged by size:
2751 2081 1404 47
StuI AGG'CCT
Cuts at: 1517 2099 1517
Size: 582 5701
StyI C'CwwG_G
Cuts at: 513 1065 3409 3722 513
Size: 552 2344 313 3074
Fragments arranged by size:
3074 2344 552 313
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4310 4327 4480 4665 5110 325
Size: 1255 2730 17 153 185 445 1498
Fragments arranged by size:
2730 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2076 2742 4529
Size: 274 80 33 51 1443 99 666 1787
Cuts at: 4529 6241 96
Size: 1712 138
Fragments arranged by size:
1787 1712 1443 666 274 138 99 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6243 6275 6243
Size: 32 6251
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6283
XbaI T'CTAG_A
Cuts at: 3619 3619
Size: 6283
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6283
XmaI C'CCGG_G
Cuts at: 1870 2976 3608 1870
Size: 1106 632 4545
Fragments arranged by size:
4545 1106 632
XmnI GAAnn'nnTTC
Cuts at: 3264 3513 4412 3264
Size: 249 899 5135
Fragments arranged by size:
5135 899 249
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4091 276
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PacI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI