Restriction map for
pFN21AE4127
(Circular) (Six-base) MAPSORT of: pFN21AE4127.seq Check: 9600 from: 1 to: 6265
pFN21AE4127 6265 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3608 1937
Size: 1671 4594
Acc65I G'GTAC_C
Cuts at: 3105 3586 3105
Size: 481 5784
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 2402 4328 5712 5980 1632
Size: 470 300 1926 1384 268 1917
Fragments arranged by size:
1926 1917 1384 470 300 268
AfeI AGC'GCT
Cuts at: 2080 2080
Size: 6265
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 5164 6141 1203
Size: 3961 977 1327
Fragments arranged by size:
3961 1327 977
AgeI A'CCGG_T
Cuts at: 2509 5096 2509
Size: 2587 3678
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 3209 1166
Size: 2043 4222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2516 2548 2516
Size: 32 6233
AlwNI CAG_nnn'CTG
Cuts at: 4008 5580 4008
Size: 1572 4693
ApaI G_GGCC'C
Cuts at: 2726 3270 2726
Size: 544 5721
ApaLI G'TGCA_C
Cuts at: 2972 4322 5478 2972
Size: 1350 1156 3759
Fragments arranged by size:
3759 1350 1156
ApoI r'AATT_y
Cuts at: 1493 3574 3800 3952 6061 1493
Size: 2081 226 152 2109 1697
Fragments arranged by size:
2109 2081 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 2891 3271 3308 3325 3337 3590 1870
Size: 73 948 380 37 17 12 253 4545
Fragments arranged by size:
4545 948 380 253 73 37 17 12
BamHI G'GATC_C
Cuts at: 3595 3962 3595
Size: 367 5898
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2207 2263 2636 2692 2819
Size: 325 217 627 420 56 373 56 127
Cuts at: 2819 3105 3330 3586 5041 618
Size: 286 225 256 1455 1842
Fragments arranged by size:
1842 1455 627 420 373 325 286 256
225 217 127 56 56
BanII G_rGCy'C
Cuts at: 1391 2163 2726 2930 3270 3392 3584 1391
Size: 772 563 204 340 122 192 4072
Fragments arranged by size:
4072 772 563 340 204 192 122
BbeI G_GCGC'C
Cuts at: 2211 2267 2640 3334 2211
Size: 56 373 694 5142
Fragments arranged by size:
5142 694 373 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2109 928
Size: 971 210 5084
Fragments arranged by size:
5084 971 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2872 2906 3419 3453 4456
Size: 34 50 34 851 34 513 34 1003
Cuts at: 4456 4490 1903
Size: 34 3678
Fragments arranged by size:
3678 1003 851 513 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2174 2282 2385
Size: 107 122 71 631 605 502 108 103
Cuts at: 2385 2480 3396 4876 136
Size: 95 916 1480 1525
Fragments arranged by size:
1525 1480 916 631 605 502 122 108
107 103 95 71
BglII A'GATC_T
Cuts at: 6260 6260
Size: 6265
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 2421 2697 2726 2824 2976 3270 3453 4326
Size: 631 276 29 98 152 294 183 873
Cuts at: 4326 5482 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 873 631 294 276 183 152
98 29
BmgBI CAC'GTC
Cuts at: 1318 2560 1318
Size: 1242 5023
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3996 4954 442
Size: 416 53 83 399 24 2579 958 1753
Fragments arranged by size:
2579 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2620 3365 2620
Size: 745 5520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3335 4925 1239
Size: 2096 1590 2579
Fragments arranged by size:
2579 2096 1590
Bpu10I CC'TnA_GC
Cuts at: 2082 2082
Size: 6265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2497 4390 5255 5553 2497
Size: 1893 865 298 3209
Fragments arranged by size:
3209 1893 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4928 882
Size: 617 3429 2219
Fragments arranged by size:
3429 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2434 4056 493
Size: 711 1230 1622 2702
Fragments arranged by size:
2702 1622 1230 711
BsaBI GATnn'nnATC
Cuts at: 6259 6259
Size: 6265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2208 2264 2303 2340
Size: 53 83 186 936 675 56 39 37
Cuts at: 2340 2637 2961 3331 4072 4454 5089 275
Size: 297 324 370 741 382 635 1451
Fragments arranged by size:
1451 936 741 675 635 382 370 324
297 186 83 56 53 39 37
BsaWI w'CCGG_w
Cuts at: 2509 4698 5096 5370 5517 5928 2509
Size: 2189 398 274 147 411 2846
Fragments arranged by size:
2846 2189 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2127 2157 1178
Size: 30 165 30 724 30 5286
Fragments arranged by size:
5286 724 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2864 3211 3220 3223 3226 3229 3232 1186
Size: 1678 347 9 3 3 3 3 4219
Fragments arranged by size:
4219 1678 347 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2307 3263 5468 5964 2307
Size: 956 2205 496 2608
Fragments arranged by size:
2608 2205 956 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3504 1163
Size: 573 1768 3924
Fragments arranged by size:
3924 1768 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2226 2274 3715 4476 5504 1540
Size: 275 186 225 48 1441 761 1028 2301
Fragments arranged by size:
2301 1441 1028 761 275 225 186 48
BsiHKAI G_wGCw'C
Cuts at: 2433 2601 2976 3216 4326 4411 5482 2433
Size: 168 375 240 1110 85 1071 3216
Fragments arranged by size:
3216 1110 1071 375 240 168 85
BsmI GAATG_Cn'
Cuts at: 3776 3869 3776
Size: 93 6172
BsmBI CGTCTCn'nnnn_
Cuts at: 2852 2852
Size: 6265
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2421 2433 2601 2697 2726 2824
Size: 399 373 258 12 168 96 29 98
Cuts at: 2824 2930 2976 3216 3270 3392 3453 3584 4326
Size: 106 46 240 54 122 61 131 742
Cuts at: 4326 4411 5482 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 373 258 240 168
131 122 106 98 96 85 61 54
46 29 12
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 4154 5884 4154
Size: 1730 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3620 844
Size: 2776 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3285 3349 4152 1394
Size: 429 1462 64 803 3507
Fragments arranged by size:
3507 1462 803 429 64
BsrDI GCAATG_nn'
Cuts at: 65 1248 4760 4934 65
Size: 1183 3512 174 1396
Fragments arranged by size:
3512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2221 2239 2509 4909 5096 1700
Size: 110 411 18 270 2400 187 2869
Fragments arranged by size:
2869 2400 411 270 187 110 18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6265
BssHII G'CGCG_C
Cuts at: 1925 2110 2843 1925
Size: 185 733 5347
Fragments arranged by size:
5347 733 185
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3417 794
Size: 2623 3642
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 2674 1184
Size: 1490 4775
BstYI r'GATC_y
Cuts at: 1972 2886 3595 3962 4347 4364 5816 5902 5914
Size: 914 709 367 385 17 1452 86 12
Cuts at: 5914 6260 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 914 709 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2172 2259 2400 3300 513
Size: 552 1107 87 141 900 3478
Fragments arranged by size:
3478 1107 900 552 141 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2851 487
Size: 683 56 1625 3901
Fragments arranged by size:
3901 1625 683 56
BtsI GCAGTG_nn'
Cuts at: 3781 4575 4595 3781
Size: 794 20 5451
Fragments arranged by size:
5451 794 20
ClaI AT'CG_AT
Cuts at: 1554 2943 1554
Size: 1389 4876
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DraIII CAC_nnn'GTG
Cuts at: 2406 2406
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 2271 2436
Size: 54 1425 50 275 18 393 48 165
Cuts at: 2436 2562 2668 3156 3315 3712 4601 6230 8
Size: 126 106 488 159 397 889 1629 43
Fragments arranged by size:
1629 1425 889 488 397 393 275 165
159 126 106 54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2223 2271 3712 1537
Size: 275 411 48 1441 4090
Fragments arranged by size:
4090 1441 411 275 48
EarI CTCTTCn'nnn_
Cuts at: 1353 3221 3250 4195 1353
Size: 1868 29 945 3423
Fragments arranged by size:
3423 1868 945 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3238 4852 5226 5372 387
Size: 1064 138 1649 1614 374 146 1280
Fragments arranged by size:
1649 1614 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2402 3335 4328 4925 5712 5980
Size: 393 470 300 933 993 597 787 268
Cuts at: 5980 1239
Size: 1524
Fragments arranged by size:
1524 993 933 787 597 470 393 300
268
EcoO109I rG'GnC_Cy
Cuts at: 2723 3061 3267 3305 2723
Size: 338 206 38 5683
Fragments arranged by size:
5683 338 206 38
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FspI TGC'GCA
Cuts at: 2876 4771 2876
Size: 1895 4370
FspAI rTGC'GCAy
Cuts at: 2876 2876
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 2082 2211 2267 2640 3334 5412 1678
Size: 404 129 56 373 694 2078 2531
Fragments arranged by size:
2531 2078 694 404 373 129 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4906 4938 4980 5012 1373
Size: 32 135 32 3334 32 42 32 2626
Fragments arranged by size:
3334 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3609 3855 669
Size: 988 281 1671 246 3079
Fragments arranged by size:
3079 1671 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KasI G'GCGC_C
Cuts at: 2207 2263 2636 3330 2207
Size: 56 373 694 5142
Fragments arranged by size:
5142 694 373 56
KpnI G_GTAC'C
Cuts at: 3109 3590 3109
Size: 481 5784
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2982 5379 5563 1305
Size: 1677 2397 184 2007
Fragments arranged by size:
2397 2007 1677 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2564 10
Size: 54 1425 343 732 3711
Fragments arranged by size:
3711 1425 732 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2684 2870 3209 4223 4582 4741 518
Size: 648 1518 186 339 1014 359 159 2042
Fragments arranged by size:
2042 1518 1014 648 359 339 186 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2261 2594 3175 3302 3681 4358 5506
Size: 516 139 333 581 127 379 677 1148
Cuts at: 5506 5751 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 581 516 379 333 245
139 127
NaeI GCC'GGC
Cuts at: 2223 2241 2223
Size: 18 6247
NarI GG'CG_CC
Cuts at: 2208 2264 2637 3331 2208
Size: 56 373 694 5142
Fragments arranged by size:
5142 694 373 56
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5713
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NgoMIV G'CCGG_C
Cuts at: 2221 2239 2221
Size: 18 6247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 3623 5168 1132
Size: 97 2394 1545 2229
Fragments arranged by size:
2394 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6265
PciI A'CATG_T
Cuts at: 5164 5164
Size: 6265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6265
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PmlI CAC'GTG
Cuts at: 2434 2434
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3305 3305
Size: 6265
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6265
PsiI TTA'TAA
Cuts at: 3835 3835
Size: 6265
PspOMI G'GGCC_C
Cuts at: 2722 3266 2722
Size: 544 5721
PspXI vC'TCGA_Gb
Cuts at: 2891 2891
Size: 6265
PstI C_TGCA'G
Cuts at: 830 3617 830
Size: 2787 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
PvuII CAG'CTG
Cuts at: 2122 2122
Size: 6265
SacII CC_GC'GG
Cuts at: 2262 3303 2262
Size: 1041 5224
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SanDI GG'GwC_CC
Cuts at: 3305 3305
Size: 6265
SapI GCTCTTCn'nnn_
Cuts at: 3221 3221
Size: 6265
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 4513 6225 4513
Size: 1712 4553
SfcI C'TryA_G
Cuts at: 826 1045 3613 4748 5429 5620 826
Size: 219 2568 1135 681 191 1471
Fragments arranged by size:
2568 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2480 2480
Size: 6265
SfoI GGC'GCC
Cuts at: 2209 2265 2638 3332 2209
Size: 56 373 694 5142
Fragments arranged by size:
5142 694 373 56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 2509 1700
Size: 809 5456
SmaI CCC'GGG
Cuts at: 1872 3273 3339 3592 1872
Size: 1401 66 253 4545
Fragments arranged by size:
4545 1401 253 66
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2476 2891 4369 5270 5532 820
Size: 197 926 533 415 1478 901 262 1553
Fragments arranged by size:
1553 1478 926 901 533 415 262 197
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3623 1132
Size: 2491 3774
SspI AAT'ATT
Cuts at: 5 52 3558 4189 5
Size: 47 3506 631 2081
Fragments arranged by size:
3506 2081 631 47
StuI AGG'CCT
Cuts at: 1517 2474 1517
Size: 957 5308
StyI C'CwwG_G
Cuts at: 513 1065 3252 3704 513
Size: 552 2187 452 3074
Fragments arranged by size:
3074 2187 552 452
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2103 3062 4292 4309 4462 4647 5092
Size: 1255 523 959 1230 17 153 185 445
Cuts at: 5092 325
Size: 1498
Fragments arranged by size:
1498 1255 1230 959 523 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2895 3144 4511
Size: 274 80 33 51 1443 918 249 1367
Cuts at: 4511 6223 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1367 918 274 249 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3500 3532 6225 6257 3500
Size: 32 2693 32 3508
Fragments arranged by size:
3508 2693 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XhoI C'TCGA_G
Cuts at: 1943 2891 1943
Size: 948 5317
XmaI C'CCGG_G
Cuts at: 1870 3271 3337 3590 1870
Size: 1401 66 253 4545
Fragments arranged by size:
4545 1401 253 66
XmnI GAAnn'nnTTC
Cuts at: 4394 4394
Size: 6265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI NruI
NsiI PacI PfoI PsrI RsrII SacI SexAI SrfI
SwaI XcmI