Restriction map for
pFN21AE4125
(Circular) (Six-base) MAPSORT of: pFN21AE4125.seq Check: 7316 from: 1 to: 6244
pFN21AE4125 6244 bp
With 182 enzymes: SgfI *
March 28, 2012 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2345 2345
Size: 6244
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2334 4054 278
Size: 53 83 186 936 798 1720 2468
Fragments arranged by size:
2468 1720 936 798 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3587 1937
Size: 1650 4594
Acc65I G'GTAC_C
Cuts at: 3565 3565
Size: 6244
AclI AA'CG_TT
Cuts at: 1563 3543 4371 4744 1563
Size: 1980 828 373 3063
Fragments arranged by size:
3063 1980 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4307 5691 5959 1632
Size: 2675 1384 268 1917
Fragments arranged by size:
2675 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6047
AflIII A'CryG_T
Cuts at: 1203 2313 5143 6120 1203
Size: 1110 2830 977 1327
Fragments arranged by size:
2830 1327 1110 977
AgeI A'CCGG_T
Cuts at: 5075 5075
Size: 6244
AhdI GACnn_n'nnGTC
Cuts at: 4973 4973
Size: 6244
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6244
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6244
AlwNI CAG_nnn'CTG
Cuts at: 2140 3100 3987 5559 2140
Size: 960 887 1572 2825
Fragments arranged by size:
2825 1572 960 887
ApaI G_GGCC'C
Cuts at: 2600 2600
Size: 6244
ApaLI G'TGCA_C
Cuts at: 4301 5457 4301
Size: 1156 5088
ApoI r'AATT_y
Cuts at: 1493 3553 3779 3931 6040 1493
Size: 2060 226 152 2109 1697
Fragments arranged by size:
2109 2060 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3074 3074
Size: 6244
AseI AT'TA_AT
Cuts at: 160 1032 4798 160
Size: 872 3766 1606
Fragments arranged by size:
3766 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
AvaI C'yCGr_G
Cuts at: 1870 1943 2177 2365 2668 3221 3310 3569 1870
Size: 73 234 188 303 553 89 259 4545
Fragments arranged by size:
4545 553 303 259 234 188 89 73
BamHI G'GATC_C
Cuts at: 3574 3941 3574
Size: 367 5877
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2872 2963 3565 5020 618
Size: 325 217 627 1085 91 602 1455 1842
Fragments arranged by size:
1842 1455 1085 627 602 325 217 91
BanII G_rGCy'C
Cuts at: 1391 2160 2600 2903 3108 3141 3414 3563 1391
Size: 769 440 303 205 33 273 149 4072
Fragments arranged by size:
4072 769 440 303 273 205 149 33
BbeI G_GCGC'C
Cuts at: 2876 2967 2876
Size: 91 6153
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2800 928
Size: 971 901 4372
Fragments arranged by size:
4372 971 901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2278 2312 2671 2705 4435 4469 1903
Size: 34 341 34 359 34 1730 34 3678
Fragments arranged by size:
3678 1730 359 341 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4136 5346 4136
Size: 1210 5034
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6244
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4855 136
Size: 107 122 71 631 605 3183 1525
Fragments arranged by size:
3183 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6239 6239
Size: 6244
BlpI GC'TnA_GC
Cuts at: 2051 3479 3661 2051
Size: 1428 182 4634
Fragments arranged by size:
4634 1428 182
Bme1580I G_kGCm'C
Cuts at: 1790 2600 4305 5461 1790
Size: 810 1705 1156 2573
Fragments arranged by size:
2573 1705 1156 810
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6244
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2473 3362 3975
Size: 416 53 83 399 24 1056 889 613
Cuts at: 3975 4933 442
Size: 958 1753
Fragments arranged by size:
1753 1056 958 889 613 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2867 3428 4904 1239
Size: 1628 561 1476 2579
Fragments arranged by size:
2579 1628 1476 561
Bpu10I CC'TnA_GC
Cuts at: 3329 3341 3329
Size: 12 6232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2264 3398 4369 5234 5532 2264
Size: 1134 971 865 298 2976
Fragments arranged by size:
2976 1134 971 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4907 882
Size: 617 3408 2219
Fragments arranged by size:
3408 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3061 4035 493
Size: 711 1857 974 2702
Fragments arranged by size:
2702 1857 974 711
BsaBI GATnn'nnATC
Cuts at: 2201 6238 2201
Size: 4037 2207
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2331 2873 2964
Size: 53 83 186 936 534 264 542 91
Cuts at: 2964 4051 4433 5068 275
Size: 1087 382 635 1451
Fragments arranged by size:
1451 1087 936 635 542 534 382 264
186 91 83 53
BsaWI w'CCGG_w
Cuts at: 3441 4677 5075 5349 5496 5907 3441
Size: 1236 398 274 147 411 3778
Fragments arranged by size:
3778 1236 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2444 2474 2810 2840 3377
Size: 30 165 30 1041 30 336 30 537
Cuts at: 3377 3407 1178
Size: 30 4015
Fragments arranged by size:
4015 1041 537 336 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2465 2540 2780 2783 2786 3511 1186
Size: 1279 75 240 3 3 725 3919
Fragments arranged by size:
3919 1279 725 240 75 3 3
BseYI C'CCAG_C
Cuts at: 2753 2876 3100 5447 5943 2753
Size: 123 224 2347 496 3054
Fragments arranged by size:
3054 2347 496 224 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2041 2272 3106 1163
Size: 573 305 231 834 4301
Fragments arranged by size:
4301 834 573 305 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2147 2359 2604 3694 4455 5483
Size: 275 186 146 212 245 1090 761 1028
Cuts at: 5483 1540
Size: 2301
Fragments arranged by size:
2301 1090 1028 761 275 245 212 186
146
BsiHKAI G_wGCw'C
Cuts at: 2709 3308 3414 4305 4390 5461 2709
Size: 599 106 891 85 1071 3492
Fragments arranged by size:
3492 1071 891 599 106 85
BsmI GAATG_Cn'
Cuts at: 3755 3848 3755
Size: 93 6151
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2600 2709 2903 3108 3141 3308
Size: 399 370 440 109 194 205 33 167
Cuts at: 3308 3414 3563 4305 4390 5461 1391
Size: 106 149 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 440 399 370 205 194
167 149 109 106 85 33
BspEI T'CCGG_A
Cuts at: 3441 5907 3441
Size: 2466 3778
BspHI T'CATG_A
Cuts at: 4133 5863 4133
Size: 1730 4514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2345 2520 3599 844
Size: 1501 175 1079 3489
Fragments arranged by size:
3489 1501 1079 175
BsrBI CCG'CTC
Cuts at: 1394 1823 2548 3345 3399 4131 1394
Size: 429 725 797 54 732 3507
Fragments arranged by size:
3507 797 732 725 429 54
BsrDI GCAATG_nn'
Cuts at: 65 1248 4739 4913 65
Size: 1183 3491 174 1396
Fragments arranged by size:
3491 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4888 5075 1700
Size: 110 3078 187 2869
Fragments arranged by size:
3078 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6244
BssHII G'CGCG_C
Cuts at: 1925 2046 2398 2433 3074 1925
Size: 121 352 35 641 5095
Fragments arranged by size:
5095 641 352 121 35
BssSI C'ACGA_G
Cuts at: 4304 5316 4304
Size: 1012 5232
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2140 794
Size: 1346 4898
BstBI TT'CG_AA
Cuts at: 5048 5048
Size: 6244
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6244
BstYI r'GATC_y
Cuts at: 1972 3574 3941 4326 4343 5795 5881 5893 6239
Size: 1602 367 385 17 1452 86 12 346
Cuts at: 6239 1972
Size: 1977
Fragments arranged by size:
1977 1602 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2759 2759
Size: 6244
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5692
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2334 2455 3151 487
Size: 683 56 1108 121 696 3580
Fragments arranged by size:
3580 1108 696 683 121 56
BtsI GCAGTG_nn'
Cuts at: 3760 4554 4574 3760
Size: 794 20 5430
Fragments arranged by size:
5430 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6209
DraI TTT'AAA
Cuts at: 3548 3548
Size: 6244
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2330 5251 809
Size: 507 1014 2921 1802
Fragments arranged by size:
2921 1802 1014 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2144 2601 2925
Size: 54 1425 50 275 18 314 457 324
Cuts at: 2925 3162 3210 3691 4580 6209 8
Size: 237 48 481 889 1629 43
Fragments arranged by size:
1629 1425 889 481 457 324 314 275
237 54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2144 2601 3691 1537
Size: 275 332 457 1090 4090
Fragments arranged by size:
4090 1090 457 332 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2550 2714 3155 3179 4174 1353
Size: 1197 164 441 24 995 3423
Fragments arranged by size:
3423 1197 995 441 164 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4831 5205 5351 387
Size: 1064 138 3242 374 146 1280
Fragments arranged by size:
3242 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3412 3412
Size: 6244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2867 3428 4307 4904 5691 5959 1239
Size: 393 1235 561 879 597 787 268 1524
Fragments arranged by size:
1524 1235 879 787 597 561 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2762 3011 2762
Size: 249 5995
EcoO109I rG'GnC_Cy
Cuts at: 2370 2596 2370
Size: 226 6018
EcoRI G'AATT_C
Cuts at: 3553 3931 3553
Size: 378 5866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 6244
FspI TGC'GCA
Cuts at: 3050 4750 3050
Size: 1700 4544
HaeII r_GCGC'y
Cuts at: 1678 2101 2506 2629 2876 2967 3047 5391 1678
Size: 423 405 123 247 91 80 2344 2531
Fragments arranged by size:
2531 2344 423 405 247 123 91 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4885 4917 4959 4991 1373
Size: 32 135 32 3313 32 42 32 2626
Fragments arranged by size:
3313 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2731 3588 3834 669
Size: 988 281 793 857 246 3079
Fragments arranged by size:
3079 988 857 793 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6244
HpaI GTT'AAC
Cuts at: 3834 3834
Size: 6244
KasI G'GCGC_C
Cuts at: 2872 2963 2872
Size: 91 6153
KpnI G_GTAC'C
Cuts at: 3569 3569
Size: 6244
MfeI C'AATT_G
Cuts at: 3843 3843
Size: 6244
MluI A'CGCG_T
Cuts at: 6120 6120
Size: 6244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2174 5358 5542 1305
Size: 869 3184 184 2007
Fragments arranged by size:
3184 2007 869 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2927 3164 10
Size: 54 1425 343 1095 237 3090
Fragments arranged by size:
3090 1425 1095 343 237 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3266 4202 4561 4720 518
Size: 648 2100 936 359 159 2042
Fragments arranged by size:
2100 2042 936 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2260 2905 3120 3478 3660 4337 5485
Size: 531 123 645 215 358 182 677 1148
Cuts at: 5485 5730 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 645 531 358 245 215
182 123
NarI GG'CG_CC
Cuts at: 2873 2964 2873
Size: 91 6153
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5692
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6244
NotI GC'GGCC_GC
Cuts at: 2144 3691 2144
Size: 1547 4697
NspI r_CATG'y
Cuts at: 1132 1229 2635 3602 5147 1132
Size: 97 1406 967 1545 2229
Fragments arranged by size:
2229 1545 1406 967 97
PasI CC'CwG_GG
Cuts at: 1348 3085 1348
Size: 1737 4507
PciI A'CATG_T
Cuts at: 5143 5143
Size: 6244
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3085 3322 1108
Size: 1977 237 4030
Fragments arranged by size:
4030 1977 237
PfoI T'CCnGG_A
Cuts at: 2445 2445
Size: 6244
PmeI GTTT'AAAC
Cuts at: 3548 3548
Size: 6244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4313 4345 5826 5858 4313
Size: 32 1481 32 4699
Fragments arranged by size:
4699 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6244
PsiI TTA'TAA
Cuts at: 3814 3814
Size: 6244
PspOMI G'GGCC_C
Cuts at: 2596 2596
Size: 6244
PstI C_TGCA'G
Cuts at: 830 2517 3096 3596 830
Size: 1687 579 500 3478
Fragments arranged by size:
3478 1687 579 500
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6244
PvuII CAG'CTG
Cuts at: 3478 3478
Size: 6244
SacI G_AGCT'C
Cuts at: 3414 3414
Size: 6244
SalI G'TCGA_C
Cuts at: 1936 3586 1936
Size: 1650 4594
SapI GCTCTTCn'nnn_
Cuts at: 2714 3155 2714
Size: 441 5803
SbfI CC_TGCA'GG
Cuts at: 3596 3596
Size: 6244
ScaI AGT'ACT
Cuts at: 2225 4492 6204 2225
Size: 2267 1712 2265
Fragments arranged by size:
2267 2265 1712
SexAI A'CCwGG_T
Cuts at: 2919 2919
Size: 6244
SfcI C'TryA_G
Cuts at: 826 1045 2041 2513 3092 3592 4727 5408 5599
Size: 219 996 472 579 500 1135 681 191
Cuts at: 5599 826
Size: 1471
Fragments arranged by size:
1471 1135 996 681 579 500 472 219
191
SfoI GGC'GCC
Cuts at: 2874 2965 2874
Size: 91 6153
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6244
SmaI CCC'GGG
Cuts at: 1872 2367 3312 3571 1872
Size: 495 945 259 4545
Fragments arranged by size:
4545 945 495 259
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2279 3413 4348 5249 5511 820
Size: 197 926 336 1134 935 901 262 1553
Fragments arranged by size:
1553 1134 935 926 901 336 262 197
SnaBI TAC'GTA
Cuts at: 493 4035 493
Size: 3542 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6244
SphI G_CATG'C
Cuts at: 1132 2635 3602 1132
Size: 1503 967 3774
Fragments arranged by size:
3774 1503 967
SspI AAT'ATT
Cuts at: 5 52 4168 5
Size: 47 4116 2081
Fragments arranged by size:
4116 2081 47
StuI AGG'CCT
Cuts at: 1517 2285 1517
Size: 768 5476
StyI C'CwwG_G
Cuts at: 513 1065 3683 513
Size: 552 2618 3074
Fragments arranged by size:
3074 2618 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4271 4288 4441 4626 5071 325
Size: 1255 2691 17 153 185 445 1498
Fragments arranged by size:
2691 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2223 2460 4490
Size: 274 80 33 51 1443 246 237 2030
Cuts at: 4490 6202 96
Size: 1712 138
Fragments arranged by size:
2030 1712 1443 274 246 237 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2810 2842 3377 3409 6204 6236 2810
Size: 32 535 32 2795 32 2818
Fragments arranged by size:
2818 2795 535 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3293 1316
Size: 1977 4267
XbaI T'CTAG_A
Cuts at: 3580 3580
Size: 6244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3274 3274
Size: 6244
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6244
XmaI C'CCGG_G
Cuts at: 1870 2365 3310 3569 1870
Size: 495 945 259 4545
Fragments arranged by size:
4545 945 495 259
XmnI GAAnn'nnTTC
Cuts at: 4373 4373
Size: 6244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2332 4052 276
Size: 53 83 186 936 798 1720 2468
Fragments arranged by size:
2468 1720 936 798 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SfiI SrfI SwaI