Restriction map for
pFN21AE4123
(Circular) (Six-base) MAPSORT of: pFN21AE4123.seq Check: 1184 from: 1 to: 6238
pFN21AE4123 6238 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4048 278
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 3183 3581 1937
Size: 894 352 398 4594
Fragments arranged by size:
4594 894 398 352
Acc65I G'GTAC_C
Cuts at: 3559 3559
Size: 6238
AclI AA'CG_TT
Cuts at: 1563 4365 4738 1563
Size: 2802 373 3063
Fragments arranged by size:
3063 2802 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2765 2887 3128 4301 5685 5953 1632
Size: 1133 122 241 1173 1384 268 1917
Fragments arranged by size:
1917 1384 1173 1133 268 241 122
AfeI AGC'GCT
Cuts at: 2644 2644
Size: 6238
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6041
AflIII A'CryG_T
Cuts at: 1203 3006 3282 5137 6114 1203
Size: 1803 276 1855 977 1327
Fragments arranged by size:
1855 1803 1327 977 276
AgeI A'CCGG_T
Cuts at: 5069 5069
Size: 6238
AhdI GACnn_n'nnGTC
Cuts at: 4967 4967
Size: 6238
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6238
AlwNI CAG_nnn'CTG
Cuts at: 2231 3981 5553 2231
Size: 1750 1572 2916
Fragments arranged by size:
2916 1750 1572
ApaLI G'TGCA_C
Cuts at: 4295 5451 4295
Size: 1156 5082
ApoI r'AATT_y
Cuts at: 1493 2454 3547 3773 3925 6034 1493
Size: 961 1093 226 152 2109 1697
Fragments arranged by size:
2109 1697 1093 961 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4792 160
Size: 872 3760 1606
Fragments arranged by size:
3760 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
AvaI C'yCGr_G
Cuts at: 1870 1943 3095 3563 1870
Size: 73 1152 468 4545
Fragments arranged by size:
4545 1152 468 73
BamHI G'GATC_C
Cuts at: 3568 3935 3568
Size: 367 5871
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2460 3559 5014 618
Size: 325 217 627 673 1099 1455 1842
Fragments arranged by size:
1842 1455 1099 673 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3557 1391
Size: 2166 4072
BbeI G_GCGC'C
Cuts at: 2464 2464
Size: 6238
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3040 928
Size: 971 1141 4126
Fragments arranged by size:
4126 1141 971
BbvCI CC'TCA_GC
Cuts at: 3192 3192
Size: 6238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4429 4463 1903
Size: 34 2492 34 3678
Fragments arranged by size:
3678 2492 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4130 5340 4130
Size: 1210 5028
BclI T'GATC_A
Cuts at: 1548 2268 1548
Size: 720 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2118 4849 136
Size: 107 122 71 631 605 446 2731 1525
Fragments arranged by size:
2731 1525 631 605 446 122 107 71
BglII A'GATC_T
Cuts at: 6233 6233
Size: 6238
BlpI GC'TnA_GC
Cuts at: 3655 3655
Size: 6238
Bme1580I G_kGCm'C
Cuts at: 1790 2998 3290 3320 4299 5455 1790
Size: 1208 292 30 979 1156 2573
Fragments arranged by size:
2573 1208 1156 979 292 30
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2406 3969 4927
Size: 416 53 83 399 24 989 1563 958
Cuts at: 4927 442
Size: 1753
Fragments arranged by size:
1753 1563 989 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6238
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3182 3182
Size: 6238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2729 3157 4898 1239
Size: 1490 428 1741 2579
Fragments arranged by size:
2579 1741 1490 428
Bpu10I CC'TnA_GC
Cuts at: 2232 3192 2232
Size: 960 5278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3454 4363 5228 5526 3454
Size: 909 865 298 4166
Fragments arranged by size:
4166 909 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2291 2337 2671 2751 4901 882
Size: 617 792 46 334 80 2150 2219
Fragments arranged by size:
2219 2150 792 617 334 80 46
BsaAI yAC'GTr
Cuts at: 493 1204 4029 493
Size: 711 2825 2702
Fragments arranged by size:
2825 2702 711
BsaBI GATnn'nnATC
Cuts at: 2159 6232 2159
Size: 4073 2165
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2461 3113 4045 4427
Size: 53 83 186 936 928 652 932 382
Cuts at: 4427 5062 275
Size: 635 1451
Fragments arranged by size:
1451 936 932 928 652 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2868 4671 5069 5343 5490 5901 2868
Size: 1803 398 274 147 411 3205
Fragments arranged by size:
3205 1803 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2918 2948 3116 3146 1178
Size: 30 165 30 1515 30 168 30 4270
Fragments arranged by size:
4270 1515 168 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2606 1186
Size: 1420 4818
BseYI C'CCAG_C
Cuts at: 2282 2301 2464 2515 2885 5441 5937 2282
Size: 19 163 51 370 2556 496 2583
Fragments arranged by size:
2583 2556 496 370 163 51 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3198 1163
Size: 573 1462 4203
Fragments arranged by size:
4203 1462 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3688 4449 5477 1540
Size: 275 186 1687 761 1028 2301
Fragments arranged by size:
2301 1687 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2868 4299 4384 5455 2868
Size: 1431 85 1071 3651
Fragments arranged by size:
3651 1431 1071 85
BsmI GAATG_Cn'
Cuts at: 3225 3749 3842 3225
Size: 524 93 5621
Fragments arranged by size:
5621 524 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2177 2177
Size: 6238
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2868 2998 3290 3320 3557 4299 4384
Size: 399 1078 130 292 30 237 742 85
Cuts at: 4384 5455 1391
Size: 1071 2174
Fragments arranged by size:
2174 1078 1071 742 399 292 237 130
85 30
BspEI T'CCGG_A
Cuts at: 5901 5901
Size: 6238
BspHI T'CATG_A
Cuts at: 2913 4127 5857 2913
Size: 1214 1730 3294
Fragments arranged by size:
3294 1730 1214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2843 3593 844
Size: 1999 750 3489
Fragments arranged by size:
3489 1999 750
BsrBI CCG'CTC
Cuts at: 1394 1823 4125 1394
Size: 429 2302 3507
Fragments arranged by size:
3507 2302 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2277 3330 3348 4733 4907 65
Size: 1183 1029 1053 18 1385 174 1396
Fragments arranged by size:
1396 1385 1183 1053 1029 174 18
BsrFI r'CCGG_y
Cuts at: 1700 1810 2121 2406 4882 5069 1700
Size: 110 311 285 2476 187 2869
Fragments arranged by size:
2869 2476 311 285 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6238
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6238
BssSI C'ACGA_G
Cuts at: 4298 5310 4298
Size: 1012 5226
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6238
BstBI TT'CG_AA
Cuts at: 5042 5042
Size: 6238
BstEII G'GTnAC_C
Cuts at: 1184 3253 1184
Size: 2069 4169
BstXI CCAn_nnnn'nTGG
Cuts at: 2447 2447
Size: 6238
BstYI r'GATC_y
Cuts at: 1972 2154 3090 3568 3935 4320 4337 5789 5875
Size: 182 936 478 367 385 17 1452 86
Cuts at: 5875 5887 6233 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 936 478 385 367 346 182
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3023 3023
Size: 6238
BtgI C'CryG_G
Cuts at: 513 1065 2004 2529 2661 513
Size: 552 939 525 132 4090
Fragments arranged by size:
4090 939 552 525 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5499
Fragments arranged by size:
5499 683 56
BtsI GCAGTG_nn'
Cuts at: 2576 2714 3255 3754 4548 4568 2576
Size: 138 541 499 794 20 4246
Fragments arranged by size:
4246 794 541 499 138 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6203
DraI TTT'AAA
Cuts at: 3542 3542
Size: 6238
DraIII CAC_nnn'GTG
Cuts at: 3324 3324
Size: 6238
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5245 809
Size: 507 3929 1802
Fragments arranged by size:
3929 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2658 3015 3685
Size: 54 1425 50 275 18 828 357 670
Cuts at: 3685 4574 6203 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 828 670 357 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3685 1537
Size: 275 1873 4090
Fragments arranged by size:
4090 1873 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 3155 3173 4168 1353
Size: 717 1085 18 995 3423
Fragments arranged by size:
3423 1085 995 717 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3023 4825 5199 5345 387
Size: 1064 138 1434 1802 374 146 1280
Fragments arranged by size:
1802 1434 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2729 2765 2887 3128 3157 4301 4898
Size: 393 1097 36 122 241 29 1144 597
Cuts at: 4898 5685 5953 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1144 1097 787 597 393 268 241
122 36 29
EcoO109I rG'GnC_Cy
Cuts at: 2228 3340 2228
Size: 1112 5126
EcoRI G'AATT_C
Cuts at: 3547 3925 3547
Size: 378 5860
EcoRV GAT'ATC
Cuts at: 2485 2485
Size: 6238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2914 2914
Size: 6238
FseI GG_CCGG'CC
Cuts at: 2125 2125
Size: 6238
FspI TGC'GCA
Cuts at: 4744 4744
Size: 6238
HaeII r_GCGC'y
Cuts at: 1678 2464 2646 5385 1678
Size: 786 182 2739 2531
Fragments arranged by size:
2739 2531 786 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2486 2518 2918
Size: 32 135 32 515 32 367 32 400
Cuts at: 2918 2950 3194 3226 4879 4911 4953 4985 1373
Size: 32 244 32 1653 32 42 32 2626
Fragments arranged by size:
2626 1653 515 400 367 244 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3582 3828 669
Size: 988 281 1644 246 3079
Fragments arranged by size:
3079 1644 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2198 748
Size: 1450 4788
HpaI GTT'AAC
Cuts at: 3828 3828
Size: 6238
KasI G'GCGC_C
Cuts at: 2460 2460
Size: 6238
KpnI G_GTAC'C
Cuts at: 3563 3563
Size: 6238
MfeI C'AATT_G
Cuts at: 3837 3837
Size: 6238
MluI A'CGCG_T
Cuts at: 6114 6114
Size: 6238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2169 3402 5352 5536 1305
Size: 864 1233 1950 184 2007
Fragments arranged by size:
2007 1950 1233 864 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3017 10
Size: 54 1425 343 1185 3231
Fragments arranged by size:
3231 1425 1185 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4196 4555 4714 518
Size: 648 843 2187 359 159 2042
Fragments arranged by size:
2187 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2263 3654 4331 5479 5724 1606
Size: 657 1391 677 1148 245 2120
Fragments arranged by size:
2120 1391 1148 677 657 245
NaeI GCC'GGC
Cuts at: 2123 2123
Size: 6238
NarI GG'CG_CC
Cuts at: 2461 2461
Size: 6238
NcoI C'CATG_G
Cuts at: 513 1065 2004 2529 513
Size: 552 939 525 4222
Fragments arranged by size:
4222 939 552 525
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6238
NgoMIV G'CCGG_C
Cuts at: 2121 2121
Size: 6238
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6238
NotI GC'GGCC_GC
Cuts at: 3685 3685
Size: 6238
NspI r_CATG'y
Cuts at: 1132 1229 3010 3286 3596 5141 1132
Size: 97 1781 276 310 1545 2229
Fragments arranged by size:
2229 1781 1545 310 276 97
PasI CC'CwG_GG
Cuts at: 1348 2225 3299 1348
Size: 877 1074 4287
Fragments arranged by size:
4287 1074 877
PciI A'CATG_T
Cuts at: 3006 3282 5137 3006
Size: 276 1855 4107
Fragments arranged by size:
4107 1855 276
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6238
PfoI T'CCnGG_A
Cuts at: 2203 2541 2727 2976 2203
Size: 338 186 249 5465
Fragments arranged by size:
5465 338 249 186
PmeI GTTT'AAAC
Cuts at: 3542 3542
Size: 6238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 5820 5852 4307
Size: 32 1481 32 4693
Fragments arranged by size:
4693 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3340 3340
Size: 6238
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6238
PsiI TTA'TAA
Cuts at: 3808 3808
Size: 6238
PstI C_TGCA'G
Cuts at: 830 3090 3590 830
Size: 2260 500 3478
Fragments arranged by size:
3478 2260 500
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6238
SalI G'TCGA_C
Cuts at: 1936 3580 1936
Size: 1644 4594
SapI GCTCTTCn'nnn_
Cuts at: 2070 2070
Size: 6238
SbfI CC_TGCA'GG
Cuts at: 3590 3590
Size: 6238
ScaI AGT'ACT
Cuts at: 4486 6198 4486
Size: 1712 4526
SfcI C'TryA_G
Cuts at: 826 1045 3086 3267 3586 4721 5402 5593 826
Size: 219 2041 181 319 1135 681 191 1471
Fragments arranged by size:
2041 1471 1135 681 319 219 191 181
SfoI GGC'GCC
Cuts at: 2462 2462
Size: 6238
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6238
SmaI CCC'GGG
Cuts at: 1872 3565 1872
Size: 1693 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3469 4342 5243 5505 820
Size: 197 926 1526 873 901 262 1553
Fragments arranged by size:
1553 1526 926 901 873 262 197
SnaBI TAC'GTA
Cuts at: 493 4029 493
Size: 3536 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6238
SphI G_CATG'C
Cuts at: 1132 3596 1132
Size: 2464 3774
SspI AAT'ATT
Cuts at: 5 52 4162 5
Size: 47 4110 2081
Fragments arranged by size:
4110 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6238
StyI C'CwwG_G
Cuts at: 513 1065 2004 2529 3078 3677 513
Size: 552 939 525 549 599 3074
Fragments arranged by size:
3074 939 599 552 549 525
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4265 4282 4435 4620 5065 325
Size: 1255 2685 17 153 185 445 1498
Fragments arranged by size:
2685 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4484 6196 96
Size: 274 80 33 51 1443 2507 1712 138
Fragments arranged by size:
2507 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6198 6230 6198
Size: 32 6206
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6238
XbaI T'CTAG_A
Cuts at: 3574 3574
Size: 6238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2704 3124 2704
Size: 420 5818
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6238
XmaI C'CCGG_G
Cuts at: 1870 3563 1870
Size: 1693 4545
XmnI GAAnn'nnTTC
Cuts at: 4367 4367
Size: 6238
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4046 276
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BstZ17I EcoICRI EcoNI FspAI NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SrfI SwaI