Restriction map for
pFN21AE4120
(Circular) (Six-base) MAPSORT of: pFN21AE4120.seq Check: 7262 from: 1 to: 6214
pFN21AE4120 6214 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3134 3134
Size: 6214
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4024 278
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3233 3370 3557 1937
Size: 1296 137 187 4594
Fragments arranged by size:
4594 1296 187 137
Acc65I G'GTAC_C
Cuts at: 3535 3535
Size: 6214
AclI AA'CG_TT
Cuts at: 1563 4341 4714 1563
Size: 2778 373 3063
Fragments arranged by size:
3063 2778 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2029 2868 4277 5661 5929 1632
Size: 397 839 1409 1384 268 1917
Fragments arranged by size:
1917 1409 1384 839 397 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6017
AflIII A'CryG_T
Cuts at: 1203 2184 5113 6090 1203
Size: 981 2929 977 1327
Fragments arranged by size:
2929 1327 981 977
AgeI A'CCGG_T
Cuts at: 5045 5045
Size: 6214
AhdI GACnn_n'nnGTC
Cuts at: 4943 4943
Size: 6214
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6214
AlwNI CAG_nnn'CTG
Cuts at: 2368 3957 5529 2368
Size: 1589 1572 3053
Fragments arranged by size:
3053 1589 1572
ApaLI G'TGCA_C
Cuts at: 4271 5427 4271
Size: 1156 5058
ApoI r'AATT_y
Cuts at: 1493 3523 3749 3901 6010 1493
Size: 2030 226 152 2109 1697
Fragments arranged by size:
2109 2030 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2391 4768 160
Size: 872 1359 2377 1606
Fragments arranged by size:
2377 1606 1359 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
AvaI C'yCGr_G
Cuts at: 1870 1943 3420 3539 1870
Size: 73 1477 119 4545
Fragments arranged by size:
4545 1477 119 73
BamHI G'GATC_C
Cuts at: 3544 3911 3544
Size: 367 5847
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3535 4990 618
Size: 325 217 627 1748 1455 1842
Fragments arranged by size:
1842 1748 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2140 2634 3491 3533 1391
Size: 749 494 857 42 4072
Fragments arranged by size:
4072 857 749 494 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2874 928
Size: 971 975 4268
Fragments arranged by size:
4268 975 971
BbvCI CC'TCA_GC
Cuts at: 2400 3432 2400
Size: 1032 5182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4405 4439 1903
Size: 34 2468 34 3678
Fragments arranged by size:
3678 2468 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4106 5316 4106
Size: 1210 5004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6214
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2897 2940 4825
Size: 107 122 71 631 605 1225 43 1885
Cuts at: 4825 136
Size: 1525
Fragments arranged by size:
1885 1525 1225 631 605 122 107 71
43
BglII A'GATC_T
Cuts at: 6209 6209
Size: 6214
BlpI GC'TnA_GC
Cuts at: 3631 3631
Size: 6214
Bme1580I G_kGCm'C
Cuts at: 1790 4275 5431 1790
Size: 2485 1156 2573
Fragments arranged by size:
2573 2485 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6214
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3513 3945 4903
Size: 416 53 83 399 24 2096 432 958
Cuts at: 4903 442
Size: 1753
Fragments arranged by size:
2096 1753 958 432 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2390 2436 2774 2808 3272 3398 4874 1239
Size: 1151 46 338 34 464 126 1476 2579
Fragments arranged by size:
2579 1476 1151 464 338 126 46 34
Bpu10I CC'TnA_GC
Cuts at: 2060 2400 2492 3009 3432 2060
Size: 340 92 517 423 4842
Fragments arranged by size:
4842 517 423 340 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4339 5204 5502 4339
Size: 865 298 5051
Fragments arranged by size:
5051 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2440 4877 882
Size: 617 941 2437 2219
Fragments arranged by size:
2437 2219 941 617
BsaAI yAC'GTr
Cuts at: 493 1204 4005 493
Size: 711 2801 2702
Fragments arranged by size:
2801 2702 711
BsaBI GATnn'nnATC
Cuts at: 6208 6208
Size: 6214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4021 4403 5038 275
Size: 53 83 186 936 2488 382 635 1451
Fragments arranged by size:
2488 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3105 4647 5045 5319 5466 5877 3105
Size: 1542 398 274 147 411 3442
Fragments arranged by size:
3442 1542 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2880 2910 3380 3410 3451
Size: 30 165 30 1477 30 470 30 41
Cuts at: 3451 3481 1178
Size: 30 3911
Fragments arranged by size:
3911 1477 470 165 41 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2591 2771 1186
Size: 1405 180 4629
Fragments arranged by size:
4629 1405 180
BseYI C'CCAG_C
Cuts at: 3017 5417 5913 3017
Size: 2400 496 3318
Fragments arranged by size:
3318 2400 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2826 1163
Size: 573 1090 4551
Fragments arranged by size:
4551 1090 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3664 4425 5453 1540
Size: 275 186 1663 761 1028 2301
Fragments arranged by size:
2301 1663 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4275 4360 5431 4275
Size: 85 1071 5058
Fragments arranged by size:
5058 1071 85
BsmI GAATG_Cn'
Cuts at: 2376 3725 3818 2376
Size: 1349 93 4772
Fragments arranged by size:
4772 1349 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2099 3125 2099
Size: 1026 5188
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2634 3491 3533 4275 4360 5431
Size: 399 350 494 857 42 742 85 1071
Cuts at: 5431 1391
Size: 2174
Fragments arranged by size:
2174 1071 857 742 494 399 350 85
42
BspEI T'CCGG_A
Cuts at: 5877 5877
Size: 6214
BspHI T'CATG_A
Cuts at: 4103 5833 4103
Size: 1730 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3134 3569 844
Size: 2290 435 3489
Fragments arranged by size:
3489 2290 435
BsrBI CCG'CTC
Cuts at: 1394 1823 2487 3097 4101 1394
Size: 429 664 610 1004 3507
Fragments arranged by size:
3507 1004 664 610 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3048 4709 4883 65
Size: 1183 1800 1661 174 1396
Fragments arranged by size:
1800 1661 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2523 4858 5045 1700
Size: 110 713 2335 187 2869
Fragments arranged by size:
2869 2335 713 187 110
BsrGI T'GTAC_A
Cuts at: 96 2357 96
Size: 2261 3953
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6214
BssSI C'ACGA_G
Cuts at: 4274 5286 4274
Size: 1012 5202
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3135 794
Size: 2341 3873
BstBI TT'CG_AA
Cuts at: 5018 5018
Size: 6214
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6214
BstYI r'GATC_y
Cuts at: 1972 2364 3544 3911 4296 4313 5765 5851 5863
Size: 392 1180 367 385 17 1452 86 12
Cuts at: 5863 6209 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1180 392 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3234 3371 3234
Size: 137 6077
Bsu36I CC'TnA_GG
Cuts at: 2574 2574
Size: 6214
BtgI C'CryG_G
Cuts at: 513 1065 2004 2689 2823 2845 513
Size: 552 939 685 134 22 3882
Fragments arranged by size:
3882 939 685 552 134 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5475
Fragments arranged by size:
5475 683 56
BtsI GCAGTG_nn'
Cuts at: 2920 2950 3730 4524 4544 2920
Size: 30 780 794 20 4590
Fragments arranged by size:
4590 794 780 30 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6179
DraI TTT'AAA
Cuts at: 3518 3518
Size: 6214
DraIII CAC_nnn'GTG
Cuts at: 2146 2146
Size: 6214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5221 809
Size: 507 3905 1802
Fragments arranged by size:
3905 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2903 3661 4550
Size: 54 1425 50 275 18 1073 758 889
Cuts at: 4550 6179 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1073 889 758 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3661 1537
Size: 275 1849 4090
Fragments arranged by size:
4090 1849 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3027 3432 3450 4144 1353
Size: 1674 405 18 694 3423
Fragments arranged by size:
3423 1674 694 405 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2290 2699 4801 5175 5321 387
Size: 1064 138 701 409 2102 374 146 1280
Fragments arranged by size:
2102 1280 1064 701 409 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2029 2390 2436 2774 2808 2868 3272
Size: 393 397 361 46 338 34 60 404
Cuts at: 3272 3398 4277 4874 5661 5929 1239
Size: 126 879 597 787 268 1524
Fragments arranged by size:
1524 879 787 597 404 397 393 361
338 268 126 60 46 34
EcoO109I rG'GnC_Cy
Cuts at: 3383 3383
Size: 6214
EcoRI G'AATT_C
Cuts at: 3523 3901 3523
Size: 378 5836
EcoRV GAT'ATC
Cuts at: 2299 2554 2299
Size: 255 5959
FspI TGC'GCA
Cuts at: 2081 4720 2081
Size: 2639 3575
HaeII r_GCGC'y
Cuts at: 1678 5361 1678
Size: 3683 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 2422 2454 3425
Size: 32 135 32 599 32 219 32 971
Cuts at: 3425 3451 3457 3483 4855 4887 4929 4961 1373
Size: 26 6 26 1372 32 42 32 2626
Fragments arranged by size:
2626 1372 971 599 219 135 42 32
32 32 32 32 32 26 26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2923 3558 3804 669
Size: 988 281 985 635 246 3079
Fragments arranged by size:
3079 988 985 635 281 246
HindIII A'AGCT_T
Cuts at: 748 2127 748
Size: 1379 4835
HpaI GTT'AAC
Cuts at: 3804 3804
Size: 6214
KpnI G_GTAC'C
Cuts at: 3539 3539
Size: 6214
MfeI C'AATT_G
Cuts at: 2027 3813 2027
Size: 1786 4428
MluI A'CGCG_T
Cuts at: 6090 6090
Size: 6214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2678 5328 5512 1305
Size: 1373 2650 184 2007
Fragments arranged by size:
2650 2007 1373 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4392
Fragments arranged by size:
4392 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3297 3303 4172 4531 4690 518
Size: 648 2131 6 869 359 159 2042
Fragments arranged by size:
2131 2042 869 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2318 3250 3630 4307 5455 5700 1606
Size: 712 932 380 677 1148 245 2120
Fragments arranged by size:
2120 1148 932 712 677 380 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2823 2845 513
Size: 552 939 819 22 3882
Fragments arranged by size:
3882 939 819 552 22
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6214
NotI GC'GGCC_GC
Cuts at: 3661 3661
Size: 6214
NspI r_CATG'y
Cuts at: 1132 1229 2086 2188 3181 3220 3572 5117 1132
Size: 97 857 102 993 39 352 1545 2229
Fragments arranged by size:
2229 1545 993 857 352 102 97 39
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6214
PciI A'CATG_T
Cuts at: 2184 5113 2184
Size: 2929 3285
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6214
PfoI T'CCnGG_A
Cuts at: 2175 2367 2175
Size: 192 6022
PmeI GTTT'AAAC
Cuts at: 3518 3518
Size: 6214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3086 3118 4283 4315 5796 5828 3086
Size: 32 1165 32 1481 32 3472
Fragments arranged by size:
3472 1481 1165 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6214
PsiI TTA'TAA
Cuts at: 3784 3784
Size: 6214
PstI C_TGCA'G
Cuts at: 830 3566 830
Size: 2736 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6214
PvuII CAG'CTG
Cuts at: 2318 3250 2318
Size: 932 5282
RsrII CG'GwC_CG
Cuts at: 2686 2686
Size: 6214
SalI G'TCGA_C
Cuts at: 1936 3556 1936
Size: 1620 4594
SbfI CC_TGCA'GG
Cuts at: 3566 3566
Size: 6214
ScaI AGT'ACT
Cuts at: 4462 6174 4462
Size: 1712 4502
SfcI C'TryA_G
Cuts at: 826 1045 2853 3562 4697 5378 5569 826
Size: 219 1808 709 1135 681 191 1471
Fragments arranged by size:
1808 1471 1135 709 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6214
SmaI CCC'GGG
Cuts at: 1872 3541 1872
Size: 1669 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3420 4318 5219 5481 820
Size: 197 926 1477 898 901 262 1553
Fragments arranged by size:
1553 1477 926 901 898 262 197
SnaBI TAC'GTA
Cuts at: 493 4005 493
Size: 3512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6214
SphI G_CATG'C
Cuts at: 1132 2086 3572 1132
Size: 954 1486 3774
Fragments arranged by size:
3774 1486 954
SspI AAT'ATT
Cuts at: 5 52 4138 5
Size: 47 4086 2081
Fragments arranged by size:
4086 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6214
StyI C'CwwG_G
Cuts at: 513 1065 2004 2823 2845 2892 3653 513
Size: 552 939 819 22 47 761 3074
Fragments arranged by size:
3074 939 819 761 552 47 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4241 4258 4411 4596 5041 325
Size: 1255 2661 17 153 185 445 1498
Fragments arranged by size:
2661 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2204 2357 2731
Size: 274 80 33 51 1443 227 153 374
Cuts at: 2731 4460 6172 96
Size: 1729 1712 138
Fragments arranged by size:
1729 1712 1443 374 274 227 153 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2880 2912 3380 3412 6174 6206 2880
Size: 32 468 32 2762 32 2888
Fragments arranged by size:
2888 2762 468 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6214
XbaI T'CTAG_A
Cuts at: 3550 3550
Size: 6214
XhoI C'TCGA_G
Cuts at: 1943 3420 1943
Size: 1477 4737
XmaI C'CCGG_G
Cuts at: 1870 3539 1870
Size: 1669 4545
XmnI GAAnn'nnTTC
Cuts at: 4343 4343
Size: 6214
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4022 276
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII RsrII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI EcoICRI EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI