Restriction map for
pFN21AE4117
(Circular) (Six-base) MAPSORT of: pFN21AE4117.seq Check: 8100 from: 1 to: 6187
pFN21AE4117 6187 bp
With 182 enzymes: SgfI *
March 28, 2012 13:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 1937
Size: 1593 4594
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 4314 4687 1563
Size: 2751 373 3063
Fragments arranged by size:
3063 2751 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2564 2753 2846 3256 3474 4250 5634 5902
Size: 932 189 93 410 218 776 1384 268
Cuts at: 5902 1632
Size: 1917
Fragments arranged by size:
1917 1384 932 776 410 268 218 189
93
AflII C'TTAA_G
Cuts at: 820 1017 2258 820
Size: 197 1241 4749
Fragments arranged by size:
4749 1241 197
AflIII A'CryG_T
Cuts at: 1203 3139 5086 6063 1203
Size: 1936 1947 977 1327
Fragments arranged by size:
1947 1936 1327 977
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 4916 4916
Size: 6187
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6187
AlwNI CAG_nnn'CTG
Cuts at: 3350 3930 5502 3350
Size: 580 1572 4035
Fragments arranged by size:
4035 1572 580
ApaLI G'TGCA_C
Cuts at: 4244 5400 4244
Size: 1156 5031
ApoI r'AATT_y
Cuts at: 1493 2513 3496 3722 3874 5983 1493
Size: 1020 983 226 152 2109 1697
Fragments arranged by size:
2109 1697 1020 983 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4741 160
Size: 872 3709 1606
Fragments arranged by size:
3709 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 3512 1870
Size: 73 1569 4545
Fragments arranged by size:
4545 1569 73
BamHI G'GATC_C
Cuts at: 2975 3517 3884 2975
Size: 542 367 5278
Fragments arranged by size:
5278 542 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 3508 4963 618
Size: 325 217 627 248 1473 1455 1842
Fragments arranged by size:
1842 1473 1455 627 325 248 217
BanII G_rGCy'C
Cuts at: 1391 3259 3506 1391
Size: 1868 247 4072
Fragments arranged by size:
4072 1868 247
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4378 4412 1903
Size: 34 2441 34 3678
Fragments arranged by size:
3678 2441 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4079 5289 4079
Size: 1210 4977
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4798 136
Size: 107 122 71 631 605 3126 1525
Fragments arranged by size:
3126 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6182 6182
Size: 6187
BlpI GC'TnA_GC
Cuts at: 2235 3604 2235
Size: 1369 4818
Bme1580I G_kGCm'C
Cuts at: 1790 4248 5404 1790
Size: 2458 1156 2573
Fragments arranged by size:
2573 2458 1156
BmgBI CAC'GTC
Cuts at: 1318 2827 1318
Size: 1509 4678
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3918 4876 442
Size: 416 53 83 399 24 2501 958 1753
Fragments arranged by size:
2501 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2672 3327 3399 4847 1239
Size: 1433 655 72 1448 2579
Fragments arranged by size:
2579 1448 1433 655 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2300 4312 5177 5475 2300
Size: 2012 865 298 3012
Fragments arranged by size:
3012 2012 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2307 3089 4850 882
Size: 617 808 782 1761 2219
Fragments arranged by size:
2219 1761 808 782 617
BsaAI yAC'GTr
Cuts at: 493 1204 3978 493
Size: 711 2774 2702
Fragments arranged by size:
2774 2702 711
BsaBI GATnn'nnATC
Cuts at: 6181 6181
Size: 6187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3994 4376 5011 275
Size: 53 83 186 936 2461 382 635 1451
Fragments arranged by size:
2461 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2298 4620 5018 5292 5439 5850 2298
Size: 2322 398 274 147 411 2635
Fragments arranged by size:
2635 2322 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5962
Fragments arranged by size:
5962 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2703 3107 1186
Size: 1517 404 4266
Fragments arranged by size:
4266 1517 404
BseYI C'CCAG_C
Cuts at: 2281 3099 5390 5886 2281
Size: 818 2291 496 2582
Fragments arranged by size:
2582 2291 818 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5614
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2056 3637 4398 5426 1540
Size: 275 186 55 1581 761 1028 2301
Fragments arranged by size:
2301 1581 1028 761 275 186 55
BsiHKAI G_wGCw'C
Cuts at: 4248 4333 5404 4248
Size: 85 1071 5031
Fragments arranged by size:
5031 1071 85
BsmI GAATG_Cn'
Cuts at: 3698 3791 3698
Size: 93 6094
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3259 3506 4248 4333 5404 1391
Size: 399 1469 247 742 85 1071 2174
Fragments arranged by size:
2174 1469 1071 742 399 247 85
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 3145 4076 5806 3145
Size: 931 1730 3526
Fragments arranged by size:
3526 1730 931
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3542 844
Size: 2698 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4074 1394
Size: 429 2251 3507
Fragments arranged by size:
3507 2251 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2095 2367 4682 4856 65
Size: 1183 847 272 2315 174 1396
Fragments arranged by size:
2315 1396 1183 847 272 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2670 4831 5018 1700
Size: 110 860 2161 187 2869
Fragments arranged by size:
2869 2161 860 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6187
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6187
BssSI C'ACGA_G
Cuts at: 4247 5259 4247
Size: 1012 5175
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6187
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstYI r'GATC_y
Cuts at: 1972 2975 3517 3884 4269 4286 5738 5824 5836
Size: 1003 542 367 385 17 1452 86 12
Cuts at: 5836 6182 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1003 542 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4696
Fragments arranged by size:
4696 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5448
Fragments arranged by size:
5448 683 56
BtsI GCAGTG_nn'
Cuts at: 2847 3703 4497 4517 2847
Size: 856 794 20 4517
Fragments arranged by size:
4517 856 794 20
ClaI AT'CG_AT
Cuts at: 1554 2211 1554
Size: 657 5530
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DraIII CAC_nnn'GTG
Cuts at: 2024 2024
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5194 809
Size: 507 3878 1802
Fragments arranged by size:
3878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 2834 3354
Size: 54 1425 50 275 18 223 781 520
Cuts at: 3354 3634 4523 6152 8
Size: 280 889 1629 43
Fragments arranged by size:
1629 1425 889 781 520 280 275 223
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2053 3634 1537
Size: 275 241 1581 4090
Fragments arranged by size:
4090 1581 275 241
EarI CTCTTCn'nnn_
Cuts at: 1353 2702 3108 4117 1353
Size: 1349 406 1009 3423
Fragments arranged by size:
3423 1349 1009 406
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4774 5148 5294 387
Size: 1064 138 3185 374 146 1280
Fragments arranged by size:
3185 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2564 2672 2753 2846 3256 3327 3399
Size: 393 932 108 81 93 410 71 72
Cuts at: 3399 3474 4250 4847 5634 5902 1239
Size: 75 776 597 787 268 1524
Fragments arranged by size:
1524 932 787 776 597 410 393 268
108 93 81 75 72 71
EcoO109I rG'GnC_Cy
Cuts at: 2346 3295 2346
Size: 949 5238
EcoRI G'AATT_C
Cuts at: 3496 3874 3496
Size: 378 5809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2273 2273
Size: 6187
FspI TGC'GCA
Cuts at: 4693 4693
Size: 6187
HaeII r_GCGC'y
Cuts at: 1678 5334 1678
Size: 3656 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2380 2412 2489 2521 4828
Size: 32 135 32 808 32 77 32 2307
Cuts at: 4828 4860 4902 4934 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2307 808 135 77 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3531 3777 669
Size: 988 281 1593 246 3079
Fragments arranged by size:
3079 1593 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6187
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3786 3786
Size: 6187
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2176 2476 5301 5485 1305
Size: 871 300 2825 184 2007
Fragments arranged by size:
2825 2007 871 300 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2836 3356 10
Size: 54 1425 343 1004 520 2841
Fragments arranged by size:
2841 1425 1004 520 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2523 2595 3144 4145 4504 4663 518
Size: 648 1357 72 549 1001 359 159 2042
Fragments arranged by size:
2042 1357 1001 648 549 359 159 72
MspA1I CmG'CkG
Cuts at: 1606 2239 3043 3419 3603 4280 5428 5673 1606
Size: 633 804 376 184 677 1148 245 2120
Fragments arranged by size:
2120 1148 804 677 633 376 245 184
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4696
Fragments arranged by size:
4696 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6187
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 3634 3634
Size: 6187
NspI r_CATG'y
Cuts at: 1132 1229 3545 5090 1132
Size: 97 2316 1545 2229
Fragments arranged by size:
2316 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6187
PciI A'CATG_T
Cuts at: 5086 5086
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6187
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3244 3276 4256 4288 5769 5801 3244
Size: 32 980 32 1481 32 3630
Fragments arranged by size:
3630 1481 980 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2346 3295 2346
Size: 949 5238
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6187
PsiI TTA'TAA
Cuts at: 3757 3757
Size: 6187
PstI C_TGCA'G
Cuts at: 830 2075 3539 830
Size: 1245 1464 3478
Fragments arranged by size:
3478 1464 1245
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
PvuII CAG'CTG
Cuts at: 2239 3043 3419 2239
Size: 804 376 5007
Fragments arranged by size:
5007 804 376
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SanDI GG'GwC_CC
Cuts at: 2346 2346
Size: 6187
SapI GCTCTTCn'nnn_
Cuts at: 2702 2702
Size: 6187
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 4435 6147 4435
Size: 1712 4475
SfcI C'TryA_G
Cuts at: 826 1045 2071 3535 4670 5351 5542 826
Size: 219 1026 1464 1135 681 191 1471
Fragments arranged by size:
1471 1464 1135 1026 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 3514 1872
Size: 1642 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2258 2315 4291 5192 5454 820
Size: 197 926 315 57 1976 901 262 1553
Fragments arranged by size:
1976 1553 926 901 315 262 197 57
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 3545 1132
Size: 2413 3774
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6187
StyI C'CwwG_G
Cuts at: 513 1065 2004 3626 513
Size: 552 939 1622 3074
Fragments arranged by size:
3074 1622 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4214 4231 4384 4569 5014 325
Size: 1255 2634 17 153 185 445 1498
Fragments arranged by size:
2634 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4433 6145 96
Size: 274 80 33 51 1443 2456 1712 138
Fragments arranged by size:
2456 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2811 2843 6147 6179 2811
Size: 32 3304 32 2819
Fragments arranged by size:
3304 2819 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6187
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 3512 1870
Size: 1642 4545
XmnI GAAnn'nnTTC
Cuts at: 4316 4316
Size: 6187
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI
SrfI SwaI XcmI