Restriction map for
pFN21AE4114
(Circular) (Six-base) MAPSORT of: pFN21AE4114.seq Check: 8785 from: 1 to: 6166
pFN21AE4114 6166 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2005 3020 3380 2005
Size: 1015 360 4791
Fragments arranged by size:
4791 1015 360
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3976 278
Size: 53 83 186 936 2440 2468
Fragments arranged by size:
2468 2440 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2388 3509 1937
Size: 451 1121 4594
Fragments arranged by size:
4594 1121 451
Acc65I G'GTAC_C
Cuts at: 3211 3487 3211
Size: 276 5890
AclI AA'CG_TT
Cuts at: 1563 4293 4666 1563
Size: 2730 373 3063
Fragments arranged by size:
3063 2730 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 2453 2500 3191 4229 5613 5881 1632
Size: 398 423 47 691 1038 1384 268 1917
Fragments arranged by size:
1917 1384 1038 691 423 398 268 47
AfeI AGC'GCT
Cuts at: 2996 2996
Size: 6166
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5969
AflIII A'CryG_T
Cuts at: 1203 5065 6042 1203
Size: 3862 977 1327
Fragments arranged by size:
3862 1327 977
AgeI A'CCGG_T
Cuts at: 4997 4997
Size: 6166
AhdI GACnn_n'nnGTC
Cuts at: 4895 4895
Size: 6166
AleI CACnn'nnGTG
Cuts at: 1166 2607 2948 1166
Size: 1441 341 4384
Fragments arranged by size:
4384 1441 341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2216 3416 1184
Size: 1032 1200 3934
Fragments arranged by size:
3934 1200 1032
AlwNI CAG_nnn'CTG
Cuts at: 2548 3012 3909 5481 2548
Size: 464 897 1572 3233
Fragments arranged by size:
3233 1572 897 464
ApaLI G'TGCA_C
Cuts at: 2273 4223 5379 2273
Size: 1950 1156 3060
Fragments arranged by size:
3060 1950 1156
ApoI r'AATT_y
Cuts at: 1493 3475 3701 3853 5962 1493
Size: 1982 226 152 2109 1697
Fragments arranged by size:
2109 1982 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4720 160
Size: 872 3688 1606
Fragments arranged by size:
3688 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
AvaI C'yCGr_G
Cuts at: 1870 1943 2658 3143 3207 3446 3491 1870
Size: 73 715 485 64 239 45 4545
Fragments arranged by size:
4545 715 485 239 73 64 45
BamHI G'GATC_C
Cuts at: 3496 3863 3496
Size: 367 5799
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3087 3211 3487 4942 618
Size: 325 217 627 1300 124 276 1455 1842
Fragments arranged by size:
1842 1455 1300 627 325 276 217 124
BanII G_rGCy'C
Cuts at: 1391 2082 2262 2322 2351 2412 2463 2931 3252
Size: 691 180 60 29 61 51 468 321
Cuts at: 3252 3485 1391
Size: 233 4072
Fragments arranged by size:
4072 691 468 321 233 180 61 60
51 29
BbeI G_GCGC'C
Cuts at: 3091 3091
Size: 6166
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2062 2506 928
Size: 971 163 444 4588
Fragments arranged by size:
4588 971 444 163
BbvCI CC'TCA_GC
Cuts at: 3440 3440
Size: 6166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3179 3213 4357 4391 1903
Size: 34 1242 34 1144 34 3678
Fragments arranged by size:
3678 1242 1144 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4058 5268 4058
Size: 1210 4956
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6166
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3123 3427 4777
Size: 107 122 71 631 605 1451 304 1350
Cuts at: 4777 136
Size: 1525
Fragments arranged by size:
1525 1451 1350 631 605 304 122 107
71
BglII A'GATC_T
Cuts at: 6161 6161
Size: 6166
BlpI GC'TnA_GC
Cuts at: 2291 3583 2291
Size: 1292 4874
Bme1580I G_kGCm'C
Cuts at: 1790 2277 3110 4227 5383 1790
Size: 487 833 1117 1156 2573
Fragments arranged by size:
2573 1156 1117 833 487
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6166
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3064 3897 4855
Size: 416 53 83 399 24 1647 833 958
Cuts at: 4855 442
Size: 1753
Fragments arranged by size:
1753 1647 958 833 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2084 2606 2660 2734 2945 4826 1239
Size: 815 30 522 54 74 211 1881 2579
Fragments arranged by size:
2579 1881 815 522 211 74 54 30
Bpu10I CC'TnA_GC
Cuts at: 2991 3440 2991
Size: 449 5717
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2876 4291 5156 5454 2876
Size: 1415 865 298 3588
Fragments arranged by size:
3588 1415 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4829 882
Size: 617 3330 2219
Fragments arranged by size:
3330 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3957 493
Size: 711 2753 2702
Fragments arranged by size:
2753 2702 711
BsaBI GATnn'nnATC
Cuts at: 6160 6160
Size: 6166
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3088 3973 4355 4990
Size: 53 83 186 936 1555 885 382 635
Cuts at: 4990 275
Size: 1451
Fragments arranged by size:
1555 1451 936 885 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2280 2484 2727 2862 4599 4997 5271 5418 5829
Size: 204 243 135 1737 398 274 147 411
Cuts at: 5829 2280
Size: 2617
Fragments arranged by size:
2617 1737 411 398 274 243 204 147
135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5941
Fragments arranged by size:
5941 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 2582 1186
Size: 1234 162 4770
Fragments arranged by size:
4770 1234 162
BseYI C'CCAG_C
Cuts at: 2344 2520 2970 3422 5369 5865 2344
Size: 176 450 452 1947 496 2645
Fragments arranged by size:
2645 1947 496 452 450 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2534 2972 3242 1163
Size: 573 798 438 270 4087
Fragments arranged by size:
4087 798 573 438 270
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3046 3298 3317 3616 4377 5405
Size: 275 186 1045 252 19 299 761 1028
Cuts at: 5405 1540
Size: 2301
Fragments arranged by size:
2301 1045 1028 761 299 275 252 186
19
BsiHKAI G_wGCw'C
Cuts at: 2277 2322 2412 2463 2931 3150 4227 4312 5383
Size: 45 90 51 468 219 1077 85 1071
Cuts at: 5383 2277
Size: 3060
Fragments arranged by size:
3060 1077 1071 468 219 90 85 51
45
BsmI GAATG_Cn'
Cuts at: 2789 3677 3770 2789
Size: 888 93 5185
Fragments arranged by size:
5185 888 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2215 2383 2215
Size: 168 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2262 2277 2322 2351 2412 2463
Size: 399 292 180 15 45 29 61 51
Cuts at: 2463 2931 3110 3150 3252 3485 4227 4312 5383
Size: 468 179 40 102 233 742 85 1071
Cuts at: 5383 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 468 399 292 233 180
179 102 85 61 51 45 40 29
15
BspEI T'CCGG_A
Cuts at: 2727 2862 5829 2727
Size: 135 2967 3064
Fragments arranged by size:
3064 2967 135
BspHI T'CATG_A
Cuts at: 4055 5785 4055
Size: 1730 4436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2005 3020 3264 3318 3380 3521 844
Size: 1161 1015 244 54 62 141 3489
Fragments arranged by size:
3489 1161 1015 244 141 62 54
BsrBI CCG'CTC
Cuts at: 1394 1823 2290 2665 3319 4053 1394
Size: 429 467 375 654 734 3507
Fragments arranged by size:
3507 734 654 467 429 375
BsrDI GCAATG_nn'
Cuts at: 65 1248 2710 4661 4835 65
Size: 1183 1462 1951 174 1396
Fragments arranged by size:
1951 1462 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 2172 3227 4810 4997 1700
Size: 110 230 132 1055 1583 187 2869
Fragments arranged by size:
2869 1583 1055 230 187 132 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6166
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6166
BssSI C'ACGA_G
Cuts at: 3380 4226 5238 3380
Size: 846 1012 4308
Fragments arranged by size:
4308 1012 846
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2118 794
Size: 1324 4842
BstBI TT'CG_AA
Cuts at: 4970 4970
Size: 6166
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6166
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 3463 2062
Size: 1401 4765
BstYI r'GATC_y
Cuts at: 1972 3496 3863 4248 4265 5717 5803 5815 6161
Size: 1524 367 385 17 1452 86 12 346
Cuts at: 6161 1972
Size: 1977
Fragments arranged by size:
1977 1524 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2237 2237
Size: 6166
BtgI C'CryG_G
Cuts at: 513 1065 2004 2608 2742 3028 3222 3405 513
Size: 552 939 604 134 286 194 183 3274
Fragments arranged by size:
3274 939 604 552 286 194 183 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2449 487
Size: 683 56 1223 4204
Fragments arranged by size:
4204 1223 683 56
BtsI GCAGTG_nn'
Cuts at: 2099 2907 2960 3682 4476 4496 2099
Size: 808 53 722 794 20 3769
Fragments arranged by size:
3769 808 794 722 53 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6166
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6131
DraI TTT'AAA
Cuts at: 3470 3470
Size: 6166
DraIII CAC_nnn'GTG
Cuts at: 2560 3154 2560
Size: 594 5572
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5173 809
Size: 507 3857 1802
Fragments arranged by size:
3857 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2217 3021
Size: 54 1425 50 275 18 268 119 804
Cuts at: 3021 3043 3225 3229 3314 3613 4502 6131 8
Size: 22 182 4 85 299 889 1629 43
Fragments arranged by size:
1629 1425 889 804 299 275 268 182
119 85 54 50 43 22 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3043 3314 3613 1537
Size: 275 1231 271 299 4090
Fragments arranged by size:
4090 1231 299 275 271
EarI CTCTTCn'nnn_
Cuts at: 1353 2864 4096 1353
Size: 1511 1232 3423
Fragments arranged by size:
3423 1511 1232
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2519 4753 5127 5273 387
Size: 1064 138 930 2234 374 146 1280
Fragments arranged by size:
2234 1280 1064 930 374 146 138
EcoICRI GAG'CTC
Cuts at: 2320 2410 2461 2929 2320
Size: 90 51 468 5557
Fragments arranged by size:
5557 468 90 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2054 2084 2453 2500 2606 2660
Size: 393 398 24 30 369 47 106 54
Cuts at: 2660 2734 2945 3191 4229 4826 5613 5881 1239
Size: 74 211 246 1038 597 787 268 1524
Fragments arranged by size:
1524 1038 787 597 398 393 369 268
246 211 106 74 54 47 30 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2822 3173 2822
Size: 351 5815
EcoO109I rG'GnC_Cy
Cuts at: 2251 3138 2251
Size: 887 5279
EcoRI G'AATT_C
Cuts at: 3475 3853 3475
Size: 378 5788
FseI GG_CCGG'CC
Cuts at: 3231 3231
Size: 6166
FspI TGC'GCA
Cuts at: 4672 4672
Size: 6166
HaeII r_GCGC'y
Cuts at: 1678 2998 3091 5313 1678
Size: 1320 93 2222 2531
Fragments arranged by size:
2531 2222 1320 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2584 2616 4807 4839 4881
Size: 32 135 32 1012 32 2191 32 42
Cuts at: 4881 4913 1373
Size: 32 2626
Fragments arranged by size:
2626 2191 1012 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3510 3756 669
Size: 988 281 1572 246 3079
Fragments arranged by size:
3079 1572 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6166
HpaI GTT'AAC
Cuts at: 3756 3756
Size: 6166
KasI G'GCGC_C
Cuts at: 3087 3087
Size: 6166
KpnI G_GTAC'C
Cuts at: 3215 3491 3215
Size: 276 5890
MfeI C'AATT_G
Cuts at: 3765 3765
Size: 6166
MluI A'CGCG_T
Cuts at: 6042 6042
Size: 6166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 2877 5280 5464 1305
Size: 1182 390 2403 184 2007
Fragments arranged by size:
2403 2007 1182 390 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2100 2219 3023 10
Size: 54 1425 343 268 119 804 3153
Fragments arranged by size:
3153 1425 804 343 268 119 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2145 2607 2948 4124 4483 4642 518
Size: 648 979 462 341 1176 359 159 2042
Fragments arranged by size:
2042 1176 979 648 462 359 341 159
MspA1I CmG'CkG
Cuts at: 1606 2520 2580 2638 2974 3030 3426 3582 4259
Size: 914 60 58 336 56 396 156 677
Cuts at: 4259 5407 5652 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 914 677 396 336 245 156
60 58 56
NaeI GCC'GGC
Cuts at: 2174 3229 2174
Size: 1055 5111
NarI GG'CG_CC
Cuts at: 3088 3088
Size: 6166
NcoI C'CATG_G
Cuts at: 513 1065 2004 2742 3405 513
Size: 552 939 738 663 3274
Fragments arranged by size:
3274 939 738 663 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6166
NgoMIV G'CCGG_C
Cuts at: 2172 3227 2172
Size: 1055 5111
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6166
NotI GC'GGCC_GC
Cuts at: 3043 3314 3613 3043
Size: 271 299 5596
Fragments arranged by size:
5596 299 271
NspI r_CATG'y
Cuts at: 1132 1229 2635 3524 5069 1132
Size: 97 1406 889 1545 2229
Fragments arranged by size:
2229 1545 1406 889 97
PasI CC'CwG_GG
Cuts at: 1348 2246 2650 1348
Size: 898 404 4864
Fragments arranged by size:
4864 898 404
PciI A'CATG_T
Cuts at: 5065 5065
Size: 6166
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2560 2743 1108
Size: 902 550 183 4531
Fragments arranged by size:
4531 902 550 183
PfoI T'CCnGG_A
Cuts at: 2541 2922 3363 2541
Size: 381 441 5344
Fragments arranged by size:
5344 441 381
PmeI GTTT'AAAC
Cuts at: 3470 3470
Size: 6166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4235 4267 5748 5780 4235
Size: 32 1481 32 4621
Fragments arranged by size:
4621 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2251 3138 2251
Size: 887 5279
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6166
PsiI TTA'TAA
Cuts at: 3736 3736
Size: 6166
PstI C_TGCA'G
Cuts at: 830 2094 3518 830
Size: 1264 1424 3478
Fragments arranged by size:
3478 1424 1264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6166
PvuII CAG'CTG
Cuts at: 2520 2638 2520
Size: 118 6048
SacI G_AGCT'C
Cuts at: 2322 2412 2463 2931 2322
Size: 90 51 468 5557
Fragments arranged by size:
5557 468 90 51
SacII CC_GC'GG
Cuts at: 3031 3031
Size: 6166
SalI G'TCGA_C
Cuts at: 1936 3508 1936
Size: 1572 4594
SanDI GG'GwC_CC
Cuts at: 2251 2251
Size: 6166
SapI GCTCTTCn'nnn_
Cuts at: 2864 2864
Size: 6166
SbfI CC_TGCA'GG
Cuts at: 3518 3518
Size: 6166
ScaI AGT'ACT
Cuts at: 4414 6126 4414
Size: 1712 4454
SexAI A'CCwGG_T
Cuts at: 2556 2556
Size: 6166
SfcI C'TryA_G
Cuts at: 826 1045 2090 2389 3004 3514 4649 5330 5521
Size: 219 1045 299 615 510 1135 681 191
Cuts at: 5521 826
Size: 1471
Fragments arranged by size:
1471 1135 1045 681 615 510 299 219
191
SfoI GGC'GCC
Cuts at: 3089 3089
Size: 6166
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6166
SmaI CCC'GGG
Cuts at: 1872 2660 3209 3493 1872
Size: 788 549 284 4545
Fragments arranged by size:
4545 788 549 284
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2891 4270 5171 5433 820
Size: 197 926 948 1379 901 262 1553
Fragments arranged by size:
1553 1379 948 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3957 493
Size: 3464 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6166
SphI G_CATG'C
Cuts at: 1132 3524 1132
Size: 2392 3774
SspI AAT'ATT
Cuts at: 5 52 4090 5
Size: 47 4038 2081
Fragments arranged by size:
4038 2081 47
StuI AGG'CCT
Cuts at: 1517 2817 1517
Size: 1300 4866
StyI C'CwwG_G
Cuts at: 513 1065 2004 2262 2742 2934 3405 3605 513
Size: 552 939 258 480 192 471 200 3074
Fragments arranged by size:
3074 939 552 480 471 258 200 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2512 2931 4193 4210 4363 4548 4993
Size: 1255 932 419 1262 17 153 185 445
Cuts at: 4993 325
Size: 1498
Fragments arranged by size:
1498 1262 1255 932 445 419 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4412 6124 96
Size: 274 80 33 51 1443 2435 1712 138
Fragments arranged by size:
2435 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6126 6158 6126
Size: 32 6134
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6166
XbaI T'CTAG_A
Cuts at: 3502 3502
Size: 6166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2212 2212
Size: 6166
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6166
XmaI C'CCGG_G
Cuts at: 1870 2658 3207 3491 1870
Size: 788 549 284 4545
Fragments arranged by size:
4545 788 549 284
XmnI GAAnn'nnTTC
Cuts at: 4295 4295
Size: 6166
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3974 276
Size: 53 83 186 936 2440 2468
Fragments arranged by size:
2468 2440 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoRV FalI FspAI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SfiI SrfI SwaI