(Circular) (Six-base) MAPSORT of: pFN21AE4090.seq Check: 5077 from: 1 to: 6073 pFN21AE4090 6073 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2268 3883 278 Size: 53 83 186 936 732 1615 2468 Fragments arranged by size: 2468 1615 936 732 186 83 53 AccI GT'mk_AC Cuts at: 1937 2994 3416 1937 Size: 1057 422 4594 Fragments arranged by size: 4594 1057 422 Acc65I G'GTAC_C Cuts at: 3313 3394 3313 Size: 81 5992 AclI AA'CG_TT Cuts at: 1563 4200 4573 1563 Size: 2637 373 3063 Fragments arranged by size: 3063 2637 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4136 5520 5788 1632 Size: 2504 1384 268 1917 Fragments arranged by size: 2504 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5876 AflIII A'CryG_T Cuts at: 1203 4972 5949 1203 Size: 3769 977 1327 Fragments arranged by size: 3769 1327 977 AgeI A'CCGG_T Cuts at: 4904 4904 Size: 6073 AhdI GACnn_n'nnGTC Cuts at: 4802 4802 Size: 6073 AleI CACnn'nnGTG Cuts at: 1166 3368 1166 Size: 2202 3871 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6073 AlwNI CAG_nnn'CTG Cuts at: 3816 5388 3816 Size: 1572 4501 ApaLI G'TGCA_C Cuts at: 4130 5286 4130 Size: 1156 4917 ApoI r'AATT_y Cuts at: 1493 3382 3608 3760 5869 1493 Size: 1889 226 152 2109 1697 Fragments arranged by size: 2109 1889 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4627 160 Size: 872 3595 1606 Fragments arranged by size: 3595 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 AvaI C'yCGr_G Cuts at: 1870 1943 2351 2411 2489 3398 1870 Size: 73 408 60 78 909 4545 Fragments arranged by size: 4545 909 408 78 73 60 BamHI G'GATC_C Cuts at: 3403 3770 3403 Size: 367 5706 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2016 2184 2344 2777 3097 Size: 325 217 627 229 168 160 433 320 Cuts at: 3097 3313 3340 3394 4849 618 Size: 216 27 54 1455 1842 Fragments arranged by size: 1842 1455 627 433 325 320 229 217 216 168 160 54 27 BanII G_rGCy'C Cuts at: 1391 2420 3131 3392 1391 Size: 1029 711 261 4072 Fragments arranged by size: 4072 1029 711 261 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2260 2473 2851 928 Size: 971 361 213 378 4150 Fragments arranged by size: 4150 971 378 361 213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4264 4298 1903 Size: 34 2327 34 3678 Fragments arranged by size: 3678 2327 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3965 5175 3965 Size: 1210 4863 BclI T'GATC_A Cuts at: 1548 1548 Size: 6073 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4684 136 Size: 107 122 71 631 605 3012 1525 Fragments arranged by size: 3012 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6068 6068 Size: 6073 BlpI GC'TnA_GC Cuts at: 3490 3490 Size: 6073 Bme1580I G_kGCm'C Cuts at: 1790 2021 2780 3177 3343 4134 5290 1790 Size: 231 759 397 166 791 1156 2573 Fragments arranged by size: 2573 1156 791 759 397 231 166 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6073 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2783 3804 4762 Size: 416 53 83 399 24 1366 1021 958 Cuts at: 4762 442 Size: 1753 Fragments arranged by size: 1753 1366 1021 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2566 3296 4733 1239 Size: 1327 730 1437 2579 Fragments arranged by size: 2579 1437 1327 730 Bpu10I CC'TnA_GC Cuts at: 3318 3318 Size: 6073 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4198 5063 5361 4198 Size: 865 298 4910 Fragments arranged by size: 4910 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4736 882 Size: 617 3237 2219 Fragments arranged by size: 3237 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2254 3864 493 Size: 711 1050 1610 2702 Fragments arranged by size: 2702 1610 1050 711 BsaBI GATnn'nnATC Cuts at: 6067 6067 Size: 6073 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2202 2265 3880 4262 Size: 53 83 186 936 669 63 1615 382 Cuts at: 4262 4897 275 Size: 635 1451 Fragments arranged by size: 1615 1451 936 669 635 382 186 83 63 53 BsaWI w'CCGG_w Cuts at: 2225 4506 4904 5178 5325 5736 2225 Size: 2281 398 274 147 411 2562 Fragments arranged by size: 2562 2281 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 2633 2663 1178 Size: 30 165 30 597 30 603 30 4588 Fragments arranged by size: 4588 603 597 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2936 1186 Size: 1750 4323 BseYI C'CCAG_C Cuts at: 2095 2128 2192 2296 2938 3233 5276 5772 2095 Size: 33 64 104 642 295 2043 496 2396 Fragments arranged by size: 2396 2043 642 496 295 104 64 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5500 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2143 2182 2246 2518 3523 4284 Size: 275 186 142 39 64 272 1005 761 Cuts at: 4284 5312 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 1005 761 275 272 186 142 64 39 BsiHKAI G_wGCw'C Cuts at: 2055 2211 2420 2439 4134 4219 5290 2055 Size: 156 209 19 1695 85 1071 2838 Fragments arranged by size: 2838 1695 1071 209 156 85 19 BsmI GAATG_Cn' Cuts at: 3584 3677 3584 Size: 93 5980 BsmBI CGTCTCn'nnnn_ Cuts at: 2072 2473 2486 2636 2072 Size: 401 13 150 5509 Fragments arranged by size: 5509 401 150 13 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2055 2211 2420 2439 2780 3131 Size: 399 231 34 156 209 19 341 351 Cuts at: 3131 3177 3343 3392 4134 4219 5290 1391 Size: 46 166 49 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 351 341 231 209 166 156 85 49 46 34 19 BspEI T'CCGG_A Cuts at: 5736 5736 Size: 6073 BspHI T'CATG_A Cuts at: 3962 5692 3962 Size: 1730 4343 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3428 844 Size: 2584 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2044 2260 2567 3960 1394 Size: 429 221 216 307 1393 3507 Fragments arranged by size: 3507 1393 429 307 221 216 BsrDI GCAATG_nn' Cuts at: 65 1248 3141 4568 4742 65 Size: 1183 1893 1427 174 1396 Fragments arranged by size: 1893 1427 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2181 2187 2245 2552 4717 4904 1700 Size: 110 371 6 58 307 2165 187 2869 Fragments arranged by size: 2869 2165 371 307 187 110 58 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6073 BssHII G'CGCG_C Cuts at: 1925 2087 2132 2159 2429 1925 Size: 162 45 27 270 5569 Fragments arranged by size: 5569 270 162 45 27 BssSI C'ACGA_G Cuts at: 4133 5145 4133 Size: 1012 5061 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6073 BstBI TT'CG_AA Cuts at: 4877 4877 Size: 6073 BstEII G'GTnAC_C Cuts at: 1184 3361 1184 Size: 2177 3896 BstXI CCAn_nnnn'nTGG Cuts at: 2228 2594 2824 2228 Size: 366 230 5477 Fragments arranged by size: 5477 366 230 BstYI r'GATC_y Cuts at: 1972 3403 3770 4155 4172 5624 5710 5722 6068 Size: 1431 367 385 17 1452 86 12 346 Cuts at: 6068 1972 Size: 1977 Fragments arranged by size: 1977 1452 1431 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2012 2137 513 Size: 552 939 8 125 4449 Fragments arranged by size: 4449 939 552 125 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5334 Fragments arranged by size: 5334 683 56 BtsI GCAGTG_nn' Cuts at: 3589 4383 4403 3589 Size: 794 20 5259 Fragments arranged by size: 5259 794 20 ClaI AT'CG_AT Cuts at: 1554 2739 1554 Size: 1185 4888 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6038 DraI TTT'AAA Cuts at: 3377 3377 Size: 6073 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5080 809 Size: 507 3764 1802 Fragments arranged by size: 3764 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2140 2243 Size: 54 1425 50 275 18 231 79 103 Cuts at: 2243 2826 3520 4409 6038 8 Size: 583 694 889 1629 43 Fragments arranged by size: 1629 1425 889 694 583 275 231 103 79 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2140 2243 3520 1537 Size: 275 328 103 1277 4090 Fragments arranged by size: 4090 1277 328 275 103 EarI CTCTTCn'nnn_ Cuts at: 1353 2271 2966 3222 4003 1353 Size: 918 695 256 781 3423 Fragments arranged by size: 3423 918 781 695 256 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4660 5034 5180 387 Size: 1064 138 3071 374 146 1280 Fragments arranged by size: 3071 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2418 2418 Size: 6073 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2566 3296 4136 4733 5520 5788 1239 Size: 393 934 730 840 597 787 268 1524 Fragments arranged by size: 1524 934 840 787 730 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2276 2324 2276 Size: 48 6025 EcoO109I rG'GnC_Cy Cuts at: 2125 2893 2125 Size: 768 5305 EcoRI G'AATT_C Cuts at: 3382 3760 3382 Size: 378 5695 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2728 2728 Size: 6073 FspI TGC'GCA Cuts at: 4579 4579 Size: 6073 HaeII r_GCGC'y Cuts at: 1678 2359 2991 5220 1678 Size: 681 632 2229 2531 Fragments arranged by size: 2531 2229 681 632 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4714 4746 4788 4820 1373 Size: 32 135 32 3142 32 42 32 2626 Fragments arranged by size: 3142 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3010 3417 3663 669 Size: 988 281 1072 407 246 3079 Fragments arranged by size: 3079 1072 988 407 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6073 HpaI GTT'AAC Cuts at: 3663 3663 Size: 6073 KpnI G_GTAC'C Cuts at: 3317 3398 3317 Size: 81 5992 MfeI C'AATT_G Cuts at: 3672 3672 Size: 6073 MluI A'CGCG_T Cuts at: 5949 5949 Size: 6073 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5187 5371 1305 Size: 3882 184 2007 Fragments arranged by size: 3882 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2828 10 Size: 54 1425 343 996 3255 Fragments arranged by size: 3255 1425 996 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2969 3368 4031 4390 4549 518 Size: 648 1803 399 663 359 159 2042 Fragments arranged by size: 2042 1803 663 648 399 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2139 2389 2572 3489 4166 5314 5559 Size: 408 125 250 183 917 677 1148 245 Cuts at: 5559 1606 Size: 2120 Fragments arranged by size: 2120 1148 917 677 408 250 245 183 125 NaeI GCC'GGC Cuts at: 2247 2554 2247 Size: 307 5766 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4582 Fragments arranged by size: 4582 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6073 NgoMIV G'CCGG_C Cuts at: 2245 2552 2245 Size: 307 5766 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6073 NotI GC'GGCC_GC Cuts at: 2140 3520 2140 Size: 1380 4693 NspI r_CATG'y Cuts at: 1132 1229 3259 3431 4976 1132 Size: 97 2030 172 1545 2229 Fragments arranged by size: 2229 2030 1545 172 97 PasI CC'CwG_GG Cuts at: 1348 3347 1348 Size: 1999 4074 PciI A'CATG_T Cuts at: 4972 4972 Size: 6073 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6073 PfoI T'CCnGG_A Cuts at: 2106 2271 2106 Size: 165 5908 PmeI GTTT'AAAC Cuts at: 3377 3377 Size: 6073 PmlI CAC'GTG Cuts at: 2254 2254 Size: 6073 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4142 4174 5655 5687 4142 Size: 32 1481 32 4528 Fragments arranged by size: 4528 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 2125 Size: 6073 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6073 PsiI TTA'TAA Cuts at: 3643 3643 Size: 6073 PstI C_TGCA'G Cuts at: 830 2545 3425 830 Size: 1715 880 3478 Fragments arranged by size: 3478 1715 880 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6073 PvuII CAG'CTG Cuts at: 2389 2389 Size: 6073 SacI G_AGCT'C Cuts at: 2420 2420 Size: 6073 SacII CC_GC'GG Cuts at: 2015 2140 2015 Size: 125 5948 SalI G'TCGA_C Cuts at: 1936 3415 1936 Size: 1479 4594 SanDI GG'GwC_CC Cuts at: 2125 2125 Size: 6073 SbfI CC_TGCA'GG Cuts at: 3425 3425 Size: 6073 ScaI AGT'ACT Cuts at: 4321 6033 4321 Size: 1712 4361 SexAI A'CCwGG_T Cuts at: 3357 3357 Size: 6073 SfcI C'TryA_G Cuts at: 826 1045 2541 3075 3421 4556 5237 5428 826 Size: 219 1496 534 346 1135 681 191 1471 Fragments arranged by size: 1496 1471 1135 681 534 346 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6073 SmaI CCC'GGG Cuts at: 1872 3400 1872 Size: 1528 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2489 4177 5078 5340 820 Size: 197 926 546 1688 901 262 1553 Fragments arranged by size: 1688 1553 926 901 546 262 197 SnaBI TAC'GTA Cuts at: 493 3864 493 Size: 3371 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6073 SphI G_CATG'C Cuts at: 1132 3431 1132 Size: 2299 3774 SspI AAT'ATT Cuts at: 5 52 3997 5 Size: 47 3945 2081 Fragments arranged by size: 3945 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6073 StyI C'CwwG_G Cuts at: 513 1065 2004 3512 513 Size: 552 939 1508 3074 Fragments arranged by size: 3074 1508 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3101 4100 4117 4270 4455 4900 325 Size: 1255 1521 999 17 153 185 445 1498 Fragments arranged by size: 1521 1498 1255 999 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4319 6031 96 Size: 274 80 33 51 1443 2342 1712 138 Fragments arranged by size: 2342 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2633 2665 2814 2846 6033 6065 2000 Size: 32 601 32 149 32 3187 32 2008 Fragments arranged by size: 3187 2008 601 149 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6073 XbaI T'CTAG_A Cuts at: 3409 3409 Size: 6073 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2935 3295 3333 2935 Size: 360 38 5675 Fragments arranged by size: 5675 360 38 XhoI C'TCGA_G Cuts at: 1943 2489 1943 Size: 546 5527 XmaI C'CCGG_G Cuts at: 1870 3398 1870 Size: 1528 4545 XmnI GAAnn'nnTTC Cuts at: 4202 4202 Size: 6073 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2266 3881 276 Size: 53 83 186 936 732 1615 2468 Fragments arranged by size: 2468 1615 936 732 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SapI SfiI SfoI SrfI SwaI