Restriction map for
pFN21AE4089
(Circular) (Six-base) MAPSORT of: pFN21AE4089.seq Check: 9043 from: 1 to: 6064
pFN21AE4089 6064 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2112 2112
Size: 6064
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3407 1937
Size: 1470 4594
Acc65I G'GTAC_C
Cuts at: 3385 3385
Size: 6064
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3033 4127 5511 5779 1632
Size: 1401 1094 1384 268 1917
Fragments arranged by size:
1917 1401 1384 1094 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 2677 4963 5940 1203
Size: 1474 2286 977 1327
Fragments arranged by size:
2286 1474 1327 977
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 4793 4793
Size: 6064
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AlwNI CAG_nnn'CTG
Cuts at: 3244 3807 5379 3244
Size: 563 1572 3929
Fragments arranged by size:
3929 1572 563
ApaI G_GGCC'C
Cuts at: 2146 2774 2929 3100 2146
Size: 628 155 171 5110
Fragments arranged by size:
5110 628 171 155
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 3373 3599 3751 5860 1493
Size: 1880 226 152 2109 1697
Fragments arranged by size:
2109 1880 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2804 2804
Size: 6064
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 2139 2364 2460 2561 2835 3093 3389
Size: 73 196 225 96 101 274 258 296
Cuts at: 3389 1870
Size: 4545
Fragments arranged by size:
4545 296 274 258 225 196 101 96
73
BamHI G'GATC_C
Cuts at: 3394 3761 3394
Size: 367 5697
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2124 2341 2702 3293 3385
Size: 325 217 627 337 217 361 591 92
Cuts at: 3385 4840 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 591 361 337 325 217
217 92
BanII G_rGCy'C
Cuts at: 1391 2146 2467 2774 2929 3100 3383 1391
Size: 755 321 307 155 171 283 4072
Fragments arranged by size:
4072 755 321 307 283 171 155
BbeI G_GCGC'C
Cuts at: 2706 2706
Size: 6064
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5093
BbvCI CC'TCA_GC
Cuts at: 2934 2934
Size: 6064
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2524 2558 4255 4289 1903
Size: 34 587 34 1697 34 3678
Fragments arranged by size:
3678 1697 587 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3956 5166 3956
Size: 1210 4854
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6064
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2294 2840 4675
Size: 107 122 71 631 605 622 546 1835
Cuts at: 4675 136
Size: 1525
Fragments arranged by size:
1835 1525 631 622 605 546 122 107
71
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 2409 3481 2409
Size: 1072 4992
Bme1580I G_kGCm'C
Cuts at: 1790 2129 2146 2461 2534 2774 2929 3100 4125
Size: 339 17 315 73 240 155 171 1025
Cuts at: 4125 5281 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1025 339 315 240 171 155
73 17
BmgBI CAC'GTC
Cuts at: 1318 2710 1318
Size: 1392 4672
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2461 3013 3795
Size: 416 53 83 399 24 1044 552 782
Cuts at: 3795 4753 442
Size: 958 1753
Fragments arranged by size:
1753 1044 958 782 552 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2690 2714 2837 2844 4724 1239
Size: 1451 24 123 7 1880 2579
Fragments arranged by size:
2579 1880 1451 123 24 7
Bpu10I CC'TnA_GC
Cuts at: 2934 2934
Size: 6064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4189 5054 5352 4189
Size: 865 298 4901
Fragments arranged by size:
4901 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2009 2169 3137 4727 882
Size: 617 510 160 968 1590 2219
Fragments arranged by size:
2219 1590 968 617 510 160
BsaAI yAC'GTr
Cuts at: 493 1204 2650 3855 493
Size: 711 1446 1205 2702
Fragments arranged by size:
2702 1446 1205 711
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2639 2703 3871 4253
Size: 53 83 186 936 1106 64 1168 382
Cuts at: 4253 4888 275
Size: 635 1451
Fragments arranged by size:
1451 1168 1106 936 635 382 186 83
64 53
BsaWI w'CCGG_w
Cuts at: 4497 4895 5169 5316 5727 4497
Size: 398 274 147 411 4834
Fragments arranged by size:
4834 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2592 2622 1178
Size: 30 165 30 1189 30 4620
Fragments arranged by size:
4620 1189 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2540 3311 1186
Size: 837 517 771 3939
Fragments arranged by size:
3939 837 771 517
BseYI C'CCAG_C
Cuts at: 2103 2179 2759 5267 5763 2103
Size: 76 580 2508 496 2404
Fragments arranged by size:
2508 2404 580 496 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2536 2630 2852 1163
Size: 573 800 94 222 4375
Fragments arranged by size:
4375 800 573 222 94
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2844 3514 4275 5303 1540
Size: 275 186 843 670 761 1028 2301
Fragments arranged by size:
2301 1028 843 761 670 275 186
BsiHKAI G_wGCw'C
Cuts at: 2386 3165 3186 4125 4210 5281 2386
Size: 779 21 939 85 1071 3169
Fragments arranged by size:
3169 1071 939 779 85 21
BsmI GAATG_Cn'
Cuts at: 3575 3668 3575
Size: 93 5971
BsmBI CGTCTCn'nnnn_
Cuts at: 2630 2630
Size: 6064
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2146 2386 2461 2467 2534 2774
Size: 399 339 17 240 75 6 67 240
Cuts at: 2774 2929 3100 3165 3186 3383 4125 4210 5281
Size: 155 171 65 21 197 742 85 1071
Cuts at: 5281 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 339 240 240 197
171 155 85 75 67 65 21 17
6
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 3953 5683 3953
Size: 1730 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 2209 2220 3342 3419 844
Size: 1268 97 11 1122 77 3489
Fragments arranged by size:
3489 1268 1122 97 77 11
BsrBI CCG'CTC
Cuts at: 1394 1823 3319 3951 1394
Size: 429 1496 632 3507
Fragments arranged by size:
3507 1496 632 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4559 4733 65
Size: 1183 3311 174 1396
Fragments arranged by size:
3311 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 2808 3285 4708 4895 1700
Size: 110 275 723 477 1423 187 2869
Fragments arranged by size:
2869 1423 723 477 275 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6064
BssHII G'CGCG_C
Cuts at: 1925 2660 2804 3262 1925
Size: 735 144 458 4727
Fragments arranged by size:
4727 735 458 144
BssSI C'ACGA_G
Cuts at: 4124 5136 4124
Size: 1012 5052
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3055 794
Size: 2261 3803
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6064
BstXI CCAn_nnnn'nTGG
Cuts at: 3174 3174
Size: 6064
BstYI r'GATC_y
Cuts at: 1972 3394 3761 4146 4163 5615 5701 5713 6059
Size: 1422 367 385 17 1452 86 12 346
Cuts at: 6059 1972
Size: 1977
Fragments arranged by size:
1977 1452 1422 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3323 3323
Size: 6064
BtgI C'CryG_G
Cuts at: 513 1065 2004 2964 513
Size: 552 939 960 3613
Fragments arranged by size:
3613 960 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2338 487
Size: 683 56 1112 4213
Fragments arranged by size:
4213 1112 683 56
BtsI GCAGTG_nn'
Cuts at: 2114 2579 3029 3076 3308 3580 4374 4394 2114
Size: 465 450 47 232 272 794 20 3784
Fragments arranged by size:
3784 794 465 450 272 232 47 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DraIII CAC_nnn'GTG
Cuts at: 2754 2754
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2183 5071 809
Size: 507 867 2888 1802
Fragments arranged by size:
2888 1802 867 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2841 2901 3511
Size: 54 1425 50 275 18 1011 60 610
Cuts at: 3511 4400 6029 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1011 889 610 275 60 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2841 3511 1537
Size: 275 1029 670 4090
Fragments arranged by size:
4090 1029 670 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2204 2238 3994 1353
Size: 851 34 1756 3423
Fragments arranged by size:
3423 1756 851 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 4651 5025 5171 387
Size: 1064 138 453 2609 374 146 1280
Fragments arranged by size:
2609 1280 1064 453 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2690 2714 2837 2844 3033 4127 4724
Size: 393 1058 24 123 7 189 1094 597
Cuts at: 4724 5511 5779 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1094 1058 787 597 393 268 189
123 24 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2216 2060
Size: 156 5908
EcoO109I rG'GnC_Cy
Cuts at: 2770 2896 2925 2938 3097 2770
Size: 126 29 13 159 5737
Fragments arranged by size:
5737 159 126 29 13
EcoRI G'AATT_C
Cuts at: 3373 3751 3373
Size: 378 5686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3128 3128
Size: 6064
FspI TGC'GCA
Cuts at: 2720 4570 2720
Size: 1850 4214
HaeII r_GCGC'y
Cuts at: 1678 2706 2857 5211 1678
Size: 1028 151 2354 2531
Fragments arranged by size:
2531 2354 1028 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2379 2411 4705 4737 4779
Size: 32 135 32 807 32 2294 32 42
Cuts at: 4779 4811 1373
Size: 32 2626
Fragments arranged by size:
2626 2294 807 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3408 3654 669
Size: 988 281 1470 246 3079
Fragments arranged by size:
3079 1470 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 3654 3654
Size: 6064
KasI G'GCGC_C
Cuts at: 2702 2702
Size: 6064
KpnI G_GTAC'C
Cuts at: 3389 3389
Size: 6064
MfeI C'AATT_G
Cuts at: 3663 3663
Size: 6064
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5178 5362 1305
Size: 3873 184 2007
Fragments arranged by size:
3873 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4242
Fragments arranged by size:
4242 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2261 2963 4022 4381 4540 518
Size: 648 1095 702 1059 359 159 2042
Fragments arranged by size:
2042 1095 1059 702 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2183 2284 2866 3235 3282 3480 4157 5305
Size: 577 101 582 369 47 198 677 1148
Cuts at: 5305 5550 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 582 577 369 245 198
101 47
NaeI GCC'GGC
Cuts at: 2810 3287 2810
Size: 477 5587
NarI GG'CG_CC
Cuts at: 2703 2703
Size: 6064
NcoI C'CATG_G
Cuts at: 513 1065 2004 2964 513
Size: 552 939 960 3613
Fragments arranged by size:
3613 960 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6064
NgoMIV G'CCGG_C
Cuts at: 2808 3285 2808
Size: 477 5587
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 2681 3422 4967 1132
Size: 97 1452 741 1545 2229
Fragments arranged by size:
2229 1545 1452 741 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 2677 4963 2677
Size: 2286 3778
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6064
PfoI T'CCnGG_A
Cuts at: 2814 2988 2814
Size: 174 5890
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PmlI CAC'GTG
Cuts at: 2650 2650
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2938 2938
Size: 6064
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6064
PsiI TTA'TAA
Cuts at: 3634 3634
Size: 6064
PspOMI G'GGCC_C
Cuts at: 2142 2770 2925 3096 2142
Size: 628 155 171 5110
Fragments arranged by size:
5110 628 171 155
PstI C_TGCA'G
Cuts at: 830 2061 2217 2786 2982 3240 3416 830
Size: 1231 156 569 196 258 176 3478
Fragments arranged by size:
3478 1231 569 258 196 176 156
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
PvuII CAG'CTG
Cuts at: 2183 2284 3235 2183
Size: 101 951 5012
Fragments arranged by size:
5012 951 101
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SfcI C'TryA_G
Cuts at: 826 1045 2057 2213 2358 2782 2978 3196 3236
Size: 219 1012 156 145 424 196 218 40
Cuts at: 3236 3412 4547 5228 5419 826
Size: 176 1135 681 191 1471
Fragments arranged by size:
1471 1135 1012 681 424 219 218 196
191 176 156 145 40
SfoI GGC'GCC
Cuts at: 2704 2704
Size: 6064
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 2141 2837 3391 1872
Size: 269 696 554 4545
Fragments arranged by size:
4545 696 554 269
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4168 5069 5331 820
Size: 197 926 2225 901 262 1553
Fragments arranged by size:
2225 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 3422 1132
Size: 2290 3774
SrfI GCCC'GGGC
Cuts at: 2837 2837
Size: 6064
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 2158 2453 1517
Size: 641 295 5128
Fragments arranged by size:
5128 641 295
StyI C'CwwG_G
Cuts at: 513 1065 2004 2505 2964 3227 3503 513
Size: 552 939 501 459 263 276 3074
Fragments arranged by size:
3074 939 552 501 459 276 263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2302 3139 4091 4108 4261 4446 4891
Size: 1255 722 837 952 17 153 185 445
Cuts at: 4891 325
Size: 1498
Fragments arranged by size:
1498 1255 952 837 722 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4310 6022 96
Size: 274 80 33 51 1443 2333 1712 138
Fragments arranged by size:
2333 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2592 2624 6024 6056 2592
Size: 32 3400 32 2600
Fragments arranged by size:
3400 2600 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6064
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2260 2587 2260
Size: 327 5737
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6064
XmaI C'CCGG_G
Cuts at: 1870 2139 2835 3389 1870
Size: 269 696 554 4545
Fragments arranged by size:
4545 696 554 269
XmnI GAAnn'nnTTC
Cuts at: 4193 4193
Size: 6064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BfrBI BplI BsiWI BstZ17I
EcoICRI EcoRV FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SwaI