Restriction map for
pFN21AE4085
(Circular) (Six-base) MAPSORT of: pFN21AE4085.seq Check: 5321 from: 1 to: 6043
pFN21AE4085 6043 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2179 2479 2583 2722 2733 2179
Size: 300 104 139 11 5489
Fragments arranged by size:
5489 300 139 104 11
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3853 278
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2622 3386 1937
Size: 685 764 4594
Fragments arranged by size:
4594 764 685
Acc65I G'GTAC_C
Cuts at: 3364 3364
Size: 6043
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4106 5490 5758 1632
Size: 2474 1384 268 1917
Fragments arranged by size:
2474 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5846
AflIII A'CryG_T
Cuts at: 1203 4942 5919 1203
Size: 3739 977 1327
Fragments arranged by size:
3739 1327 977
AgeI A'CCGG_T
Cuts at: 4874 4874
Size: 6043
AhdI GACnn_n'nnGTC
Cuts at: 4772 4772
Size: 6043
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6043
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2435 1184
Size: 1251 4792
AlwNI CAG_nnn'CTG
Cuts at: 2017 2368 3142 3786 5358 2017
Size: 351 774 644 1572 2702
Fragments arranged by size:
2702 1572 774 644 351
ApaI G_GGCC'C
Cuts at: 2329 2550 2646 3118 3274 3281 3297 2329
Size: 221 96 472 156 7 16 5075
Fragments arranged by size:
5075 472 221 156 96 16 7
ApaLI G'TGCA_C
Cuts at: 2714 2999 4100 5256 2714
Size: 285 1101 1156 3501
Fragments arranged by size:
3501 1156 1101 285
ApoI r'AATT_y
Cuts at: 1493 3352 3578 3730 5839 1493
Size: 1859 226 152 2109 1697
Fragments arranged by size:
2109 1859 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4597 160
Size: 872 3565 1606
Fragments arranged by size:
3565 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2762 3267 3273 3368 1870
Size: 73 99 720 505 6 95 4545
Fragments arranged by size:
4545 720 505 99 95 73 6
BamHI G'GATC_C
Cuts at: 3373 3740 3373
Size: 367 5676
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2354 2598 2843 3161 3175
Size: 325 217 627 567 244 245 318 14
Cuts at: 3175 3364 4819 618
Size: 189 1455 1842
Fragments arranged by size:
1842 1455 627 567 325 318 245 244
217 189 14
BanII G_rGCy'C
Cuts at: 1391 2088 2329 2484 2550 2646 2947 3118 3210
Size: 697 241 155 66 96 301 171 92
Cuts at: 3210 3261 3274 3281 3297 3362 1391
Size: 51 13 7 16 65 4072
Fragments arranged by size:
4072 697 301 241 171 155 96 92
66 65 51 16 13 7
BbeI G_GCGC'C
Cuts at: 3165 3165
Size: 6043
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5072
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2898 2932 4234 4268 1903
Size: 34 961 34 1302 34 3678
Fragments arranged by size:
3678 1302 961 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3935 5145 3935
Size: 1210 4833
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6043
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2277 3053 3301
Size: 107 122 71 631 605 605 776 248
Cuts at: 3301 4654 136
Size: 1353 1525
Fragments arranged by size:
1525 1353 776 631 605 605 248 122
107 71
BglII A'GATC_T
Cuts at: 2610 6038 2610
Size: 3428 2615
BlpI GC'TnA_GC
Cuts at: 2087 2143 3460 2087
Size: 56 1317 4670
Fragments arranged by size:
4670 1317 56
Bme1580I G_kGCm'C
Cuts at: 1790 2329 2550 2646 2718 2897 3003 3118 3274
Size: 539 221 96 72 179 106 115 156
Cuts at: 3274 3281 3297 4104 5260 1790
Size: 7 16 807 1156 2573
Fragments arranged by size:
2573 1156 807 539 221 179 156 115
106 96 72 16 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6043
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2233 2948 3774
Size: 416 53 83 399 24 816 715 826
Cuts at: 3774 4732 442
Size: 958 1753
Fragments arranged by size:
1753 958 826 816 715 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6043
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 6043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2044 2403 2951 4703 1239
Size: 805 359 548 1752 2579
Fragments arranged by size:
2579 1752 805 548 359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2480 4168 5033 5331 2480
Size: 1688 865 298 3192
Fragments arranged by size:
3192 1688 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2379 2396 4706 882
Size: 617 880 17 2310 2219
Fragments arranged by size:
2310 2219 880 617 17
BsaAI yAC'GTr
Cuts at: 493 1204 2713 3834 493
Size: 711 1509 1121 2702
Fragments arranged by size:
2702 1509 1121 711
BsaBI GATnn'nnATC
Cuts at: 6037 6037
Size: 6043
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2670 2739 3162 3850
Size: 53 83 186 936 1137 69 423 688
Cuts at: 3850 4232 4867 275
Size: 382 635 1451
Fragments arranged by size:
1451 1137 936 688 635 423 382 186
83 69 53
BsaWI w'CCGG_w
Cuts at: 2518 4476 4874 5148 5295 5706 2518
Size: 1958 398 274 147 411 2855
Fragments arranged by size:
2855 1958 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2186 2216 1178
Size: 30 165 30 783 30 5005
Fragments arranged by size:
5005 783 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2600 2993 3218 1186
Size: 1414 393 225 4011
Fragments arranged by size:
4011 1414 393 225
BseYI C'CCAG_C
Cuts at: 2890 3048 3057 3135 5246 5742 2890
Size: 158 9 78 2111 496 3191
Fragments arranged by size:
3191 2111 496 158 78 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2054 2405 2423 2627 3212 1163
Size: 573 318 351 18 204 585 3994
Fragments arranged by size:
3994 585 573 351 318 204 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2119 2519 3493 4254 5282 1540
Size: 275 186 118 400 974 761 1028 2301
Fragments arranged by size:
2301 1028 974 761 400 275 186 118
BsiHKAI G_wGCw'C
Cuts at: 2088 2496 2718 3003 4104 4189 5260 2088
Size: 408 222 285 1101 85 1071 2871
Fragments arranged by size:
2871 1101 1071 408 285 222 85
BsmI GAATG_Cn'
Cuts at: 3554 3647 3554
Size: 93 5950
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2329 2484 2496 2550 2646 2718
Size: 399 298 241 155 12 54 96 72
Cuts at: 2718 2897 2947 3003 3118 3210 3261 3274 3281
Size: 179 50 56 115 92 51 13 7
Cuts at: 3281 3297 3362 4104 4189 5260 1391
Size: 16 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 298 241 179 155
115 96 92 85 72 65 56 54
51 50 16 13 12 7
BspEI T'CCGG_A
Cuts at: 5706 5706
Size: 6043
BspHI T'CATG_A
Cuts at: 3932 5662 3932
Size: 1730 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2179 2427 2479 2583 2722 2733 2766 3036
Size: 1335 248 52 104 139 11 33 270
Cuts at: 3036 3398 844
Size: 362 3489
Fragments arranged by size:
3489 1335 362 270 248 139 104 52
33 11
BsrBI CCG'CTC
Cuts at: 1394 1823 2790 3022 3930 1394
Size: 429 967 232 908 3507
Fragments arranged by size:
3507 967 908 429 232
BsrDI GCAATG_nn'
Cuts at: 65 1248 4538 4712 65
Size: 1183 3290 174 1396
Fragments arranged by size:
3290 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4687 4874 1700
Size: 110 2877 187 2869
Fragments arranged by size:
2877 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6043
BssHII G'CGCG_C
Cuts at: 1925 2296 1925
Size: 371 5672
BssSI C'ACGA_G
Cuts at: 2441 4103 5115 2441
Size: 1662 1012 3369
Fragments arranged by size:
3369 1662 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6043
BstBI TT'CG_AA
Cuts at: 4847 4847
Size: 6043
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6043
BstXI CCAn_nnnn'nTGG
Cuts at: 2068 2068
Size: 6043
BstYI r'GATC_y
Cuts at: 1972 2610 3373 3740 4125 4142 5594 5680 5692
Size: 638 763 367 385 17 1452 86 12
Cuts at: 5692 6038 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 763 638 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2460 3171 2460
Size: 711 5332
BtgI C'CryG_G
Cuts at: 513 1065 2004 2467 2550 3218 3238 3253 513
Size: 552 939 463 83 668 20 15 3303
Fragments arranged by size:
3303 939 668 552 463 83 20 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5304
Fragments arranged by size:
5304 683 56
BtsI GCAGTG_nn'
Cuts at: 2273 2895 3559 4353 4373 2273
Size: 622 664 794 20 3943
Fragments arranged by size:
3943 794 664 622 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6008
DraI TTT'AAA
Cuts at: 3347 3347
Size: 6043
DraIII CAC_nnn'GTG
Cuts at: 3007 3007
Size: 6043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5050 809
Size: 507 3734 1802
Fragments arranged by size:
3734 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3092 3490 4379
Size: 54 1425 50 275 18 1262 398 889
Cuts at: 4379 6008 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1262 889 398 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3490 1537
Size: 275 1678 4090
Fragments arranged by size:
4090 1678 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2786 3973 1353
Size: 1433 1187 3423
Fragments arranged by size:
3423 1433 1187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2174 3092 3238 4630 5004 5150
Size: 1064 138 585 918 146 1392 374 146
Cuts at: 5150 387
Size: 1280
Fragments arranged by size:
1392 1280 1064 918 585 374 146 146
138
EcoICRI GAG'CTC
Cuts at: 2086 2086
Size: 6043
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2044 2403 2951 4106 4703 5490 5758
Size: 393 412 359 548 1155 597 787 268
Cuts at: 5758 1239
Size: 1524
Fragments arranged by size:
1524 1155 787 597 548 412 393 359
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2021 2729 2837 2021
Size: 708 108 5227
Fragments arranged by size:
5227 708 108
EcoO109I rG'GnC_Cy
Cuts at: 2014 2026 2325 2377 2416 2546 2643 2913 3025
Size: 12 299 52 39 130 97 270 112
Cuts at: 3025 3080 3114 3277 3278 3293 3294 3303 2014
Size: 55 34 163 1 15 1 9 4754
Fragments arranged by size:
4754 299 270 163 130 112 97 55
52 39 34 15 12 9 1 1
EcoRI G'AATT_C
Cuts at: 3352 3730 3352
Size: 378 5665
FspI TGC'GCA
Cuts at: 4549 4549
Size: 6043
HaeII r_GCGC'y
Cuts at: 1678 2891 3165 5190 1678
Size: 1213 274 2025 2531
Fragments arranged by size:
2531 2025 1213 274
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 4684 4716 4758
Size: 32 135 32 476 32 2604 32 42
Cuts at: 4758 4790 1373
Size: 32 2626
Fragments arranged by size:
2626 2604 476 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3387 3633 669
Size: 988 281 1449 246 3079
Fragments arranged by size:
3079 1449 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6043
HpaI GTT'AAC
Cuts at: 3633 3633
Size: 6043
KasI G'GCGC_C
Cuts at: 3161 3161
Size: 6043
KpnI G_GTAC'C
Cuts at: 3368 3368
Size: 6043
MfeI C'AATT_G
Cuts at: 3642 3642
Size: 6043
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2100 2722 2997 5157 5341 1305
Size: 795 622 275 2160 184 2007
Fragments arranged by size:
2160 2007 795 622 275 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4221
Fragments arranged by size:
4221 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4001 4360 4519 518
Size: 648 2835 359 159 2042
Fragments arranged by size:
2835 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2091 2971 3034 3052 3061 3142 3220 3240
Size: 485 880 63 18 9 81 78 20
Cuts at: 3240 3459 4136 5284 5529 1606
Size: 219 677 1148 245 2120
Fragments arranged by size:
2120 1148 880 677 485 245 219 81
78 63 20 18 9
NarI GG'CG_CC
Cuts at: 3162 3162
Size: 6043
NcoI C'CATG_G
Cuts at: 513 1065 2004 3253 513
Size: 552 939 1249 3303
Fragments arranged by size:
3303 1249 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6043
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6043
NotI GC'GGCC_GC
Cuts at: 3490 3490
Size: 6043
NspI r_CATG'y
Cuts at: 1132 1229 3401 4946 1132
Size: 97 2172 1545 2229
Fragments arranged by size:
2229 2172 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2017 2542 2630 1348
Size: 669 525 88 4761
Fragments arranged by size:
4761 669 525 88
PciI A'CATG_T
Cuts at: 4942 4942
Size: 6043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2017 3142 1108
Size: 909 1125 4009
Fragments arranged by size:
4009 1125 909
PfoI T'CCnGG_A
Cuts at: 2928 3327 2928
Size: 399 5644
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PmlI CAC'GTG
Cuts at: 2713 2713
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5625 5657 4112
Size: 32 1481 32 4498
Fragments arranged by size:
4498 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2026 2416 3025 3080 2026
Size: 390 609 55 4989
Fragments arranged by size:
4989 609 390 55
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6043
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PspOMI G'GGCC_C
Cuts at: 2325 2546 2642 3114 3270 3277 3293 2325
Size: 221 96 472 156 7 16 5075
Fragments arranged by size:
5075 472 221 156 96 16 7
PstI C_TGCA'G
Cuts at: 830 2052 2176 2490 2730 2902 2976 3033 3395
Size: 1222 124 314 240 172 74 57 362
Cuts at: 3395 830
Size: 3478
Fragments arranged by size:
3478 1222 362 314 240 172 124 74
57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
PvuII CAG'CTG
Cuts at: 2971 3052 3061 3142 2971
Size: 81 9 81 5872
Fragments arranged by size:
5872 81 81 9
SacI G_AGCT'C
Cuts at: 2088 2088
Size: 6043
SacII CC_GC'GG
Cuts at: 3221 3241 3221
Size: 20 6023
SalI G'TCGA_C
Cuts at: 1936 3385 1936
Size: 1449 4594
SanDI GG'GwC_CC
Cuts at: 3080 3080
Size: 6043
SbfI CC_TGCA'GG
Cuts at: 2490 3395 2490
Size: 905 5138
ScaI AGT'ACT
Cuts at: 2853 4291 6003 2853
Size: 1438 1712 2893
Fragments arranged by size:
2893 1712 1438
SexAI A'CCwGG_T
Cuts at: 2028 2028
Size: 6043
SfcI C'TryA_G
Cuts at: 826 1045 2048 2172 2486 2726 2898 2972 3029
Size: 219 1003 124 314 240 172 74 57
Cuts at: 3029 3391 4526 5207 5398 826
Size: 362 1135 681 191 1471
Fragments arranged by size:
1471 1135 1003 681 362 314 240 219
191 172 124 74 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 3053 3301 3053
Size: 248 5795
SfoI GGC'GCC
Cuts at: 3163 3163
Size: 6043
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6043
SmaI CCC'GGG
Cuts at: 1872 3275 3370 1872
Size: 1403 95 4545
Fragments arranged by size:
4545 1403 95
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2495 4147 5048 5310 820
Size: 197 926 552 1652 901 262 1553
Fragments arranged by size:
1652 1553 926 901 552 262 197
SnaBI TAC'GTA
Cuts at: 493 3834 493
Size: 3341 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6043
SphI G_CATG'C
Cuts at: 1132 3401 1132
Size: 2269 3774
SspI AAT'ATT
Cuts at: 5 52 3967 5
Size: 47 3915 2081
Fragments arranged by size:
3915 2081 47
StuI AGG'CCT
Cuts at: 1517 2501 2591 2984 1517
Size: 984 90 393 4576
Fragments arranged by size:
4576 984 393 90
StyI C'CwwG_G
Cuts at: 513 1065 2004 3253 3482 513
Size: 552 939 1249 229 3074
Fragments arranged by size:
3074 1249 939 552 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2181 2194 4070 4087 4240 4425 4870
Size: 1255 601 13 1876 17 153 185 445
Cuts at: 4870 325
Size: 1498
Fragments arranged by size:
1876 1498 1255 601 445 185 153 17
13
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2851 4289 6001
Size: 274 80 33 51 1443 874 1438 1712
Cuts at: 6001 96
Size: 138
Fragments arranged by size:
1712 1443 1438 874 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2186 2218 6003 6035 2186
Size: 32 3785 32 2194
Fragments arranged by size:
3785 2194 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6043
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3325 3325
Size: 6043
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6043
XmaI C'CCGG_G
Cuts at: 1870 3273 3368 1870
Size: 1403 95 4545
Fragments arranged by size:
4545 1403 95
XmnI GAAnn'nnTTC
Cuts at: 4172 4172
Size: 6043
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3851 276
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SapI
SrfI SwaI