Restriction map for
pFN21AE4083
(Circular) (Six-base) MAPSORT of: pFN21AE4083.seq Check: 591 from: 1 to: 6010
pFN21AE4083 6010 bp
With 182 enzymes: SgfI *
March 28, 2012 13:10 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3820 278
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3353 1937
Size: 1416 4594
Acc65I G'GTAC_C
Cuts at: 3331 3331
Size: 6010
AclI AA'CG_TT
Cuts at: 1563 4137 4510 1563
Size: 2574 373 3063
Fragments arranged by size:
3063 2574 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2094 4073 5457 5725 1632
Size: 462 1979 1384 268 1917
Fragments arranged by size:
1979 1917 1384 462 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5813
AflIII A'CryG_T
Cuts at: 1203 3227 4909 5886 1203
Size: 2024 1682 977 1327
Fragments arranged by size:
2024 1682 1327 977
AgeI A'CCGG_T
Cuts at: 4841 4841
Size: 6010
AhdI GACnn_n'nnGTC
Cuts at: 4739 4739
Size: 6010
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6010
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2637 2669 2887 2919 2637
Size: 32 218 32 5728
Fragments arranged by size:
5728 218 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2156 2402 3753 5325 2156
Size: 246 1351 1572 2841
Fragments arranged by size:
2841 1572 1351 246
ApaLI G'TGCA_C
Cuts at: 4067 5223 4067
Size: 1156 4854
ApoI r'AATT_y
Cuts at: 1493 2432 2520 2780 3017 3146 3319 3545 3697
Size: 939 88 260 237 129 173 226 152
Cuts at: 3697 5806 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 939 260 237 226 173 152
129 88
AseI AT'TA_AT
Cuts at: 160 1032 4564 160
Size: 872 3532 1606
Fragments arranged by size:
3532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
AvaI C'yCGr_G
Cuts at: 1870 1943 3335 1870
Size: 73 1392 4545
Fragments arranged by size:
4545 1392 73
BamHI G'GATC_C
Cuts at: 3340 3707 3340
Size: 367 5643
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2818 3331 4786 618
Size: 325 217 627 1031 513 1455 1842
Fragments arranged by size:
1842 1455 1031 627 513 325 217
BanII G_rGCy'C
Cuts at: 1391 3329 1391
Size: 1938 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2459 928
Size: 971 560 4479
Fragments arranged by size:
4479 971 560
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2108 2142 4201 4235 1903
Size: 34 50 34 87 34 2059 34 3678
Fragments arranged by size:
3678 2059 87 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2970 3902 5112 2970
Size: 932 1210 3868
Fragments arranged by size:
3868 1210 932
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6010
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4621 136
Size: 107 122 71 631 605 2949 1525
Fragments arranged by size:
2949 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6005 6005
Size: 6010
BlpI GC'TnA_GC
Cuts at: 3427 3427
Size: 6010
Bme1580I G_kGCm'C
Cuts at: 1790 2326 4071 5227 1790
Size: 536 1745 1156 2573
Fragments arranged by size:
2573 1745 1156 536
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6010
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3741 4699 442
Size: 416 53 83 399 24 2324 958 1753
Fragments arranged by size:
2324 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6010
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 6010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2133 2253 2275 2424 4670 1239
Size: 894 120 22 149 2246 2579
Fragments arranged by size:
2579 2246 894 149 120 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4135 5000 5298 4135
Size: 865 298 4847
Fragments arranged by size:
4847 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4673 882
Size: 617 3174 2219
Fragments arranged by size:
3174 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3801 493
Size: 711 2597 2702
Fragments arranged by size:
2702 2597 711
BsaBI GATnn'nnATC
Cuts at: 6004 6004
Size: 6010
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3817 4199 4834 275
Size: 53 83 186 936 2284 382 635 1451
Fragments arranged by size:
2284 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4443 4841 5115 5262 5673 4443
Size: 398 274 147 411 4780
Fragments arranged by size:
4780 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5785
Fragments arranged by size:
5785 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2074 1186
Size: 888 5122
BseYI C'CCAG_C
Cuts at: 5213 5709 5213
Size: 496 5514
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 2295 1163
Size: 573 387 172 4878
Fragments arranged by size:
4878 573 387 172
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3460 4221 5249 1540
Size: 275 186 1459 761 1028 2301
Fragments arranged by size:
2301 1459 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4071 4156 5227 4071
Size: 85 1071 4854
Fragments arranged by size:
4854 1071 85
BsmI GAATG_Cn'
Cuts at: 3521 3614 3521
Size: 93 5917
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2326 3329 4071 4156 5227 1391
Size: 399 536 1003 742 85 1071 2174
Fragments arranged by size:
2174 1071 1003 742 536 399 85
BspEI T'CCGG_A
Cuts at: 5673 5673
Size: 6010
BspHI T'CATG_A
Cuts at: 2053 3899 5629 2053
Size: 1846 1730 2434
Fragments arranged by size:
2434 1846 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3365 844
Size: 2521 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3897 1394
Size: 429 2074 3507
Fragments arranged by size:
3507 2074 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4505 4679 65
Size: 1183 3257 174 1396
Fragments arranged by size:
3257 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4654 4841 1700
Size: 110 2844 187 2869
Fragments arranged by size:
2869 2844 187 110
BsrGI T'GTAC_A
Cuts at: 96 2335 96
Size: 2239 3771
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6010
BssSI C'ACGA_G
Cuts at: 4070 5082 4070
Size: 1012 4998
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2282 794
Size: 1488 4522
BstBI TT'CG_AA
Cuts at: 4814 4814
Size: 6010
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6010
BstYI r'GATC_y
Cuts at: 1972 2645 3340 3707 4092 4109 5561 5647 5659
Size: 673 695 367 385 17 1452 86 12
Cuts at: 5659 6005 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 695 673 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2326 513
Size: 552 1261 4197
Fragments arranged by size:
4197 1261 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5271
Fragments arranged by size:
5271 683 56
BtsI GCAGTG_nn'
Cuts at: 2170 3526 4320 4340 2170
Size: 1356 794 20 3840
Fragments arranged by size:
3840 1356 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5975
DraI TTT'AAA
Cuts at: 2709 2842 3314 2709
Size: 133 472 5405
Fragments arranged by size:
5405 472 133
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2038 2089 5017 809
Size: 507 722 51 2928 1802
Fragments arranged by size:
2928 1802 722 507 51
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3457 4346 5975
Size: 54 1425 50 275 18 1627 889 1629
Cuts at: 5975 8
Size: 43
Fragments arranged by size:
1629 1627 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3457 1537
Size: 275 1645 4090
Fragments arranged by size:
4090 1645 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2450 3186 3940 1353
Size: 1097 736 754 3423
Fragments arranged by size:
3423 1097 754 736
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4597 4971 5117 387
Size: 1064 138 3008 374 146 1280
Fragments arranged by size:
3008 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2133 2253 2275 2424 4073 4670
Size: 393 462 39 120 22 149 1649 597
Cuts at: 4670 5457 5725 1239
Size: 787 268 1524
Fragments arranged by size:
1649 1524 787 597 462 393 268 149
120 39 22
EcoO109I rG'GnC_Cy
Cuts at: 2601 2601
Size: 6010
EcoRI G'AATT_C
Cuts at: 2432 3319 3697 2432
Size: 887 378 4745
Fragments arranged by size:
4745 887 378
FspI TGC'GCA
Cuts at: 4516 4516
Size: 6010
HaeII r_GCGC'y
Cuts at: 1678 5157 1678
Size: 3479 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4651 4683 4725 4757 1373
Size: 32 135 32 3079 32 42 32 2626
Fragments arranged by size:
3079 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3354 3600 669
Size: 988 281 1416 246 3079
Fragments arranged by size:
3079 1416 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6010
HpaI GTT'AAC
Cuts at: 3600 3600
Size: 6010
KpnI G_GTAC'C
Cuts at: 3335 3335
Size: 6010
MfeI C'AATT_G
Cuts at: 3609 3609
Size: 6010
MluI A'CGCG_T
Cuts at: 5886 5886
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2737 5124 5308 1305
Size: 1432 2387 184 2007
Fragments arranged by size:
2387 2007 1432 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4188
Fragments arranged by size:
4188 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2319 2331 3968 4327 4486 518
Size: 648 1153 12 1637 359 159 2042
Fragments arranged by size:
2042 1637 1153 648 359 159 12
MspA1I CmG'CkG
Cuts at: 1606 2011 3426 4103 5251 5496 1606
Size: 405 1415 677 1148 245 2120
Fragments arranged by size:
2120 1415 1148 677 405 245
NcoI C'CATG_G
Cuts at: 513 1065 2326 513
Size: 552 1261 4197
Fragments arranged by size:
4197 1261 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6010
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6010
NotI GC'GGCC_GC
Cuts at: 3457 3457
Size: 6010
NspI r_CATG'y
Cuts at: 1132 1229 3231 3368 4913 1132
Size: 97 2002 137 1545 2229
Fragments arranged by size:
2229 2002 1545 137 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6010
PciI A'CATG_T
Cuts at: 3227 4909 3227
Size: 1682 4328
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2156 3080 1108
Size: 1048 924 4038
Fragments arranged by size:
4038 1048 924
PfoI T'CCnGG_A
Cuts at: 2149 2149
Size: 6010
PmeI GTTT'AAAC
Cuts at: 3314 3314
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3091 3123 4079 4111 5592 5624 3091
Size: 32 956 32 1481 32 3477
Fragments arranged by size:
3477 1481 956 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2601 2601
Size: 6010
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6010
PsiI TTA'TAA
Cuts at: 2933 3580 2933
Size: 647 5363
PstI C_TGCA'G
Cuts at: 830 3242 3362 830
Size: 2412 120 3478
Fragments arranged by size:
3478 2412 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6010
SalI G'TCGA_C
Cuts at: 1936 3352 1936
Size: 1416 4594
SbfI CC_TGCA'GG
Cuts at: 3362 3362
Size: 6010
ScaI AGT'ACT
Cuts at: 4258 5970 4258
Size: 1712 4298
SfcI C'TryA_G
Cuts at: 826 1045 3238 3358 4493 5174 5365 826
Size: 219 2193 120 1135 681 191 1471
Fragments arranged by size:
2193 1471 1135 681 219 191 120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6010
SmaI CCC'GGG
Cuts at: 1872 3337 1872
Size: 1465 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4114 5015 5277 820
Size: 197 926 2171 901 262 1553
Fragments arranged by size:
2171 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3801 493
Size: 3308 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6010
SphI G_CATG'C
Cuts at: 1132 3368 1132
Size: 2236 3774
SspI AAT'ATT
Cuts at: 5 52 3060 3934 5
Size: 47 3008 874 2081
Fragments arranged by size:
3008 2081 874 47
StuI AGG'CCT
Cuts at: 1517 2071 1517
Size: 554 5456
StyI C'CwwG_G
Cuts at: 513 1065 2326 3449 513
Size: 552 1261 1123 3074
Fragments arranged by size:
3074 1261 1123 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4037 4054 4207 4392 4837 325
Size: 1255 2457 17 153 185 445 1498
Fragments arranged by size:
2457 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2335 4256 5968
Size: 274 80 33 51 1443 358 1921 1712
Cuts at: 5968 96
Size: 138
Fragments arranged by size:
1921 1712 1443 358 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5970 6002 5970
Size: 32 5978
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6010
XbaI T'CTAG_A
Cuts at: 3346 3346
Size: 6010
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6010
XmaI C'CCGG_G
Cuts at: 1870 3335 1870
Size: 1465 4545
XmnI GAAnn'nnTTC
Cuts at: 4139 4139
Size: 6010
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3818 276
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI