Restriction map for
pFN21AE4082
(Circular) (Six-base) MAPSORT of: pFN21AE4082.seq Check: 8678 from: 1 to: 5992
pFN21AE4082 5992 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2879 3802 278
Size: 53 83 186 936 1343 923 2468
Fragments arranged by size:
2468 1343 936 923 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2601 2879 3251 3335 1937
Size: 664 278 372 84 4594
Fragments arranged by size:
4594 664 372 278 84
Acc65I G'GTAC_C
Cuts at: 3313 3313
Size: 5992
AclI AA'CG_TT
Cuts at: 1563 4119 4492 1563
Size: 2556 373 3063
Fragments arranged by size:
3063 2556 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3094 4055 5439 5707 1632
Size: 1462 961 1384 268 1917
Fragments arranged by size:
1917 1462 1384 961 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5795
AflIII A'CryG_T
Cuts at: 1203 2424 4891 5868 1203
Size: 1221 2467 977 1327
Fragments arranged by size:
2467 1327 1221 977
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 4721 4721
Size: 5992
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5992
AlwNI CAG_nnn'CTG
Cuts at: 2668 3735 5307 2668
Size: 1067 1572 3353
Fragments arranged by size:
3353 1572 1067
ApaI G_GGCC'C
Cuts at: 2842 2842
Size: 5992
ApaLI G'TGCA_C
Cuts at: 2558 4049 5205 2558
Size: 1491 1156 3345
Fragments arranged by size:
3345 1491 1156
ApoI r'AATT_y
Cuts at: 1493 3301 3527 3679 5788 1493
Size: 1808 226 152 2109 1697
Fragments arranged by size:
2109 1808 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4546 160
Size: 872 3514 1606
Fragments arranged by size:
3514 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 2326 2381 3317 1870
Size: 73 383 55 936 4545
Fragments arranged by size:
4545 936 383 73 55
BamHI G'GATC_C
Cuts at: 3322 3689 3322
Size: 367 5625
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2284 3313 4768 618
Size: 325 217 627 497 1029 1455 1842
Fragments arranged by size:
1842 1455 1029 627 497 325 217
BanII G_rGCy'C
Cuts at: 1391 2242 2333 2474 2842 3311 1391
Size: 851 91 141 368 469 4072
Fragments arranged by size:
4072 851 469 368 141 91
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2325 2879 3087 928
Size: 971 426 554 208 3833
Fragments arranged by size:
3833 971 554 426 208
BbvCI CC'TCA_GC
Cuts at: 3265 3265
Size: 5992
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2912 2946 4183 4217 1903
Size: 34 50 34 891 34 1237 34 3678
Fragments arranged by size:
3678 1237 891 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3884 5094 3884
Size: 1210 4782
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5992
BfrBI ATG'CAT
Cuts at: 3009 3009
Size: 5992
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2246 4603 136
Size: 107 122 71 631 605 574 2357 1525
Fragments arranged by size:
2357 1525 631 605 574 122 107 71
BglII A'GATC_T
Cuts at: 5987 5987
Size: 5992
BlpI GC'TnA_GC
Cuts at: 3409 3409
Size: 5992
Bme1580I G_kGCm'C
Cuts at: 1790 2289 2562 2842 4053 5209 1790
Size: 499 273 280 1211 1156 2573
Fragments arranged by size:
2573 1211 1156 499 280 273
BmgBI CAC'GTC
Cuts at: 1318 2599 3234 1318
Size: 1281 635 4076
Fragments arranged by size:
4076 1281 635
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2048 2462 3723
Size: 416 53 83 399 24 631 414 1261
Cuts at: 3723 4681 442
Size: 958 1753
Fragments arranged by size:
1753 1261 958 631 416 414 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5992
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 3142 2674
Size: 468 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2157 2264 3209 3260 4652 1239
Size: 918 107 945 51 1392 2579
Fragments arranged by size:
2579 1392 945 918 107 51
Bpu10I CC'TnA_GC
Cuts at: 3265 3265
Size: 5992
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2498 2632 2954 4117 4982 5280 2498
Size: 134 322 1163 865 298 3210
Fragments arranged by size:
3210 1163 865 322 298 134
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2128 4655 882
Size: 617 629 2527 2219
Fragments arranged by size:
2527 2219 629 617
BsaAI yAC'GTr
Cuts at: 493 1204 2913 3783 493
Size: 711 1709 870 2702
Fragments arranged by size:
2702 1709 870 711
BsaBI GATnn'nnATC
Cuts at: 2627 5986 2627
Size: 3359 2633
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 2876 3799 4181
Size: 53 83 186 936 834 509 923 382
Cuts at: 4181 4816 275
Size: 635 1451
Fragments arranged by size:
1451 936 923 834 635 509 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2020 4425 4823 5097 5244 5655 2020
Size: 2405 398 274 147 411 2357
Fragments arranged by size:
2405 2357 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2555 2585 3233 3263 1178
Size: 30 165 30 1152 30 648 30 3907
Fragments arranged by size:
3907 1152 648 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2969 3253 1186
Size: 1783 284 3925
Fragments arranged by size:
3925 1783 284
BseYI C'CCAG_C
Cuts at: 2529 2776 3196 5195 5691 2529
Size: 247 420 1999 496 2830
Fragments arranged by size:
2830 1999 496 420 247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2485 1163
Size: 573 749 4670
Fragments arranged by size:
4670 749 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2231 2867 3442 4203 5231 1540
Size: 275 186 230 636 575 761 1028 2301
Fragments arranged by size:
2301 1028 761 636 575 275 230 186
BsiHKAI G_wGCw'C
Cuts at: 2028 2333 2562 4053 4138 5209 2028
Size: 305 229 1491 85 1071 2811
Fragments arranged by size:
2811 1491 1071 305 229 85
BsmI GAATG_Cn'
Cuts at: 3503 3596 3503
Size: 93 5899
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2242 2289 2333 2474 2562 2842
Size: 399 238 214 47 44 141 88 280
Cuts at: 2842 3311 4053 4138 5209 1391
Size: 469 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 469 399 280 238 214
141 88 85 47 44
BspEI T'CCGG_A
Cuts at: 2020 5655 2020
Size: 3635 2357
BspHI T'CATG_A
Cuts at: 3881 5611 3881
Size: 1730 4262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2676 2891 3347 844
Size: 1832 215 456 3489
Fragments arranged by size:
3489 1832 456 215
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 2463 3879 1394
Size: 429 188 452 1416 3507
Fragments arranged by size:
3507 1416 452 429 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 2475 4487 4661 65
Size: 1183 1227 2012 174 1396
Fragments arranged by size:
2012 1396 1227 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2353 2537 2866 3141 4636 4823 1700
Size: 110 543 184 329 275 1495 187 2869
Fragments arranged by size:
2869 1495 543 329 275 187 184 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5992
BssHII G'CGCG_C
Cuts at: 1925 2533 1925
Size: 608 5384
BssSI C'ACGA_G
Cuts at: 2243 2582 4052 5064 2243
Size: 339 1470 1012 3171
Fragments arranged by size:
3171 1470 1012 339
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2032 2668 794
Size: 1238 636 4118
Fragments arranged by size:
4118 1238 636
BstBI TT'CG_AA
Cuts at: 4796 4796
Size: 5992
BstEII G'GTnAC_C
Cuts at: 1184 2209 1184
Size: 1025 4967
BstXI CCAn_nnnn'nTGG
Cuts at: 2941 3238 2941
Size: 297 5695
BstYI r'GATC_y
Cuts at: 1972 2347 2973 3322 3689 4074 4091 5543 5629
Size: 375 626 349 367 385 17 1452 86
Cuts at: 5629 5641 5987 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 626 385 375 367 349 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3252 3252
Size: 5992
Bsu36I CC'TnA_GG
Cuts at: 3276 3276
Size: 5992
BtgI C'CryG_G
Cuts at: 513 1065 2214 2225 513
Size: 552 1149 11 4280
Fragments arranged by size:
4280 1149 552 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5253
Fragments arranged by size:
5253 683 56
BtsI GCAGTG_nn'
Cuts at: 2066 2304 2998 3214 3220 3508 4302 4322 2066
Size: 238 694 216 6 288 794 20 3736
Fragments arranged by size:
3736 794 694 288 238 216 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5957
DraI TTT'AAA
Cuts at: 3296 3296
Size: 5992
DraIII CAC_nnn'GTG
Cuts at: 2755 2755
Size: 5992
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2672 4999 809
Size: 507 1356 2327 1802
Fragments arranged by size:
2327 1802 1356 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2116 2222
Size: 54 1425 50 275 18 228 58 106
Cuts at: 2222 2228 2550 2864 3069 3439 4328 5957 8
Size: 6 322 314 205 370 889 1629 43
Fragments arranged by size:
1629 1425 889 370 322 314 275 228
205 106 58 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2228 2864 3439 1537
Size: 275 416 636 575 4090
Fragments arranged by size:
4090 636 575 416 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3922 1353
Size: 2569 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2477 4579 4953 5099 387
Size: 1064 138 888 2102 374 146 1280
Fragments arranged by size:
2102 1280 1064 888 374 146 138
EcoICRI GAG'CTC
Cuts at: 2331 2331
Size: 5992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2264 3094 3209 3260 4055 4652
Size: 393 525 107 830 115 51 795 597
Cuts at: 4652 5439 5707 1239
Size: 787 268 1524
Fragments arranged by size:
1524 830 795 787 597 525 393 268
115 107 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2405 2405
Size: 5992
EcoO109I rG'GnC_Cy
Cuts at: 2050 2124 2180 2443 2665 3121 3280 2050
Size: 74 56 263 222 456 159 4762
Fragments arranged by size:
4762 456 263 222 159 74 56
EcoRI G'AATT_C
Cuts at: 3301 3679 3301
Size: 378 5614
FspI TGC'GCA
Cuts at: 4498 4498
Size: 5992
HaeII r_GCGC'y
Cuts at: 1678 2360 3043 5139 1678
Size: 682 683 2096 2531
Fragments arranged by size:
2531 2096 683 682
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4633 4665 4707 4739 1373
Size: 32 135 32 3061 32 42 32 2626
Fragments arranged by size:
3061 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3336 3582 669
Size: 988 281 1398 246 3079
Fragments arranged by size:
3079 1398 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5992
HpaI GTT'AAC
Cuts at: 3582 3582
Size: 5992
KpnI G_GTAC'C
Cuts at: 3317 3317
Size: 5992
MfeI C'AATT_G
Cuts at: 3591 3591
Size: 5992
MluI A'CGCG_T
Cuts at: 2424 5868 2424
Size: 3444 2548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2117 2598 5106 5290 1305
Size: 812 481 2508 184 2007
Fragments arranged by size:
2508 2007 812 481 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2118 2224 10
Size: 54 1425 343 286 106 3778
Fragments arranged by size:
3778 1425 343 286 106 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3950 4309 4468 518
Size: 648 2784 359 159 2042
Fragments arranged by size:
2784 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3408 4085 5233 5478 1606
Size: 1802 677 1148 245 2120
Fragments arranged by size:
2120 1802 1148 677 245
NaeI GCC'GGC
Cuts at: 2355 2539 2355
Size: 184 5808
NcoI C'CATG_G
Cuts at: 513 1065 2214 513
Size: 552 1149 4291
Fragments arranged by size:
4291 1149 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5992
NgoMIV G'CCGG_C
Cuts at: 2353 2537 2353
Size: 184 5808
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5992
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NsiI A_TGCA'T
Cuts at: 3011 3011
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 3350 4895 1132
Size: 97 2121 1545 2229
Fragments arranged by size:
2229 2121 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2129 1348
Size: 781 5211
PciI A'CATG_T
Cuts at: 4891 4891
Size: 5992
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2220 2740 1108
Size: 1112 520 4360
Fragments arranged by size:
4360 1112 520
PfoI T'CCnGG_A
Cuts at: 3179 3179
Size: 5992
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PmlI CAC'GTG
Cuts at: 2913 2913
Size: 5992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4061 4093 5574 5606 4061
Size: 32 1481 32 4447
Fragments arranged by size:
4447 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2050 2180 2665 3121 2050
Size: 130 485 456 4921
Fragments arranged by size:
4921 485 456 130
PshAI GACnn'nnGTC
Cuts at: 1613 2597 1613
Size: 984 5008
PsiI TTA'TAA
Cuts at: 3562 3562
Size: 5992
PspOMI G'GGCC_C
Cuts at: 2838 2838
Size: 5992
PspXI vC'TCGA_Gb
Cuts at: 2326 2326
Size: 5992
PstI C_TGCA'G
Cuts at: 830 2855 3227 3344 830
Size: 2025 372 117 3478
Fragments arranged by size:
3478 2025 372 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5992
SacI G_AGCT'C
Cuts at: 2333 2333
Size: 5992
SalI G'TCGA_C
Cuts at: 1936 3334 1936
Size: 1398 4594
SanDI GG'GwC_CC
Cuts at: 2050 2050
Size: 5992
SbfI CC_TGCA'GG
Cuts at: 3344 3344
Size: 5992
ScaI AGT'ACT
Cuts at: 4240 5952 4240
Size: 1712 4280
SfcI C'TryA_G
Cuts at: 826 1045 2851 3103 3223 3340 4475 5156 5347
Size: 219 1806 252 120 117 1135 681 191
Cuts at: 5347 826
Size: 1471
Fragments arranged by size:
1806 1471 1135 681 252 219 191 120
117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 3319 1872
Size: 1447 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2326 2513 2611 2969 4096 4997
Size: 197 926 383 187 98 358 1127 901
Cuts at: 4997 5259 820
Size: 262 1553
Fragments arranged by size:
1553 1127 926 901 383 358 262 197
187 98
SnaBI TAC'GTA
Cuts at: 493 3783 493
Size: 3290 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 3350 1132
Size: 2218 3774
SspI AAT'ATT
Cuts at: 5 52 3916 5
Size: 47 3864 2081
Fragments arranged by size:
3864 2081 47
StuI AGG'CCT
Cuts at: 1517 2156 2846 1517
Size: 639 690 4663
Fragments arranged by size:
4663 690 639
StyI C'CwwG_G
Cuts at: 513 1065 2214 3431 513
Size: 552 1149 1217 3074
Fragments arranged by size:
3074 1217 1149 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2761 4019 4036 4189 4374 4819 325
Size: 1255 1181 1258 17 153 185 445 1498
Fragments arranged by size:
1498 1258 1255 1181 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4238 5950 96
Size: 274 80 33 51 1443 2261 1712 138
Fragments arranged by size:
2261 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5952 5984 5952
Size: 32 5960
Tth111I GACn'n_nGTC
Cuts at: 1316 2764 1316
Size: 1448 4544
XbaI T'CTAG_A
Cuts at: 3328 3328
Size: 5992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2126 2126
Size: 5992
XhoI C'TCGA_G
Cuts at: 1943 2326 1943
Size: 383 5609
XmaI C'CCGG_G
Cuts at: 1870 3317 1870
Size: 1447 4545
XmnI GAAnn'nnTTC
Cuts at: 4121 4121
Size: 5992
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2877 3800 276
Size: 53 83 186 936 1343 923 2468
Fragments arranged by size:
2468 1343 936 923 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BsmBI EcoRV FalI FseI FspAI KasI NarI NruI
PacI PsrI PvuII RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI