Restriction map for
pFN21AE4078
(Circular) (Six-base) MAPSORT of: pFN21AE4078.seq Check: 6220 from: 1 to: 5941
pFN21AE4078 5941 bp
With 182 enzymes: SgfI *
March 28, 2012 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3060 3060
Size: 5941
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3140 3751 278
Size: 53 83 186 936 1604 611 2468
Fragments arranged by size:
2468 1604 936 611 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3140 3284 1937
Size: 1203 144 4594
Fragments arranged by size:
4594 1203 144
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2366 2754 4004 5388 5656 1632
Size: 734 388 1250 1384 268 1917
Fragments arranged by size:
1917 1384 1250 734 388 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2381 3068 3210 1184
Size: 1197 687 142 3915
Fragments arranged by size:
3915 1197 687 142
AlwNI CAG_nnn'CTG
Cuts at: 2885 3684 5256 2885
Size: 799 1572 3570
Fragments arranged by size:
3570 1572 799
ApaI G_GGCC'C
Cuts at: 2146 2146
Size: 5941
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 3250 3476 3628 5737 1493
Size: 1757 226 152 2109 1697
Fragments arranged by size:
2109 1757 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 2220 3266 1870
Size: 73 106 171 1046 4545
Fragments arranged by size:
4545 1046 171 106 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 2208 2255 2447 3262
Size: 325 217 627 304 117 47 192 815
Cuts at: 3262 4717 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 815 627 325 304 217 192
117 47
BanII G_rGCy'C
Cuts at: 1391 2146 3009 3260 1391
Size: 755 863 251 4072
Fragments arranged by size:
4072 863 755 251
BbeI G_GCGC'C
Cuts at: 2095 2212 2095
Size: 117 5824
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2742 928
Size: 971 843 4127
Fragments arranged by size:
4127 971 843
BbvCI CC'TCA_GC
Cuts at: 3149 3149
Size: 5941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4132 4166 1903
Size: 34 2195 34 3678
Fragments arranged by size:
3678 2195 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2069 4552
Size: 107 122 71 631 605 337 60 2483
Cuts at: 4552 136
Size: 1525
Fragments arranged by size:
2483 1525 631 605 337 122 107 71
60
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2146 2808 4002 5158 1790
Size: 356 662 1194 1156 2573
Fragments arranged by size:
2573 1194 1156 662 356
BmgBI CAC'GTC
Cuts at: 1318 3220 1318
Size: 1902 4039
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2273 2327 2968
Size: 416 53 83 399 24 856 54 641
Cuts at: 2968 3233 3672 4630 442
Size: 265 439 958 1753
Fragments arranged by size:
1753 958 856 641 439 416 399 265
83 54 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2332 2644 4601 1239
Size: 1093 312 1957 2579
Fragments arranged by size:
2579 1957 1093 312
Bpu10I CC'TnA_GC
Cuts at: 3149 3149
Size: 5941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4066 4931 5229 4066
Size: 865 298 4778
Fragments arranged by size:
4778 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4604 882
Size: 617 3105 2219
Fragments arranged by size:
3105 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2092 2209 3103 3137
Size: 53 83 186 936 559 117 894 34
Cuts at: 3137 3748 4130 4765 275
Size: 611 382 635 1451
Fragments arranged by size:
1451 936 894 635 611 559 382 186
117 83 53 34
BsaWI w'CCGG_w
Cuts at: 2979 4374 4772 5046 5193 5604 2979
Size: 1395 398 274 147 411 3316
Fragments arranged by size:
3316 1395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2344 2374 3127 3157 1178
Size: 30 165 30 941 30 753 30 3962
Fragments arranged by size:
3962 941 753 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2042 2045 2048 2051 2054 2117 3017
Size: 853 3 3 3 3 3 63 900
Cuts at: 3017 1186
Size: 4110
Fragments arranged by size:
4110 900 853 63 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 2483 2704 2885 5144 5640 2483
Size: 221 181 2259 496 2784
Fragments arranged by size:
2784 2259 496 221 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2559 3104 1163
Size: 573 823 545 4000
Fragments arranged by size:
4000 823 573 545
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 2073 2152 3391 4152 5180
Size: 275 186 18 54 79 1239 761 1028
Cuts at: 5180 1540
Size: 2301
Fragments arranged by size:
2301 1239 1028 761 275 186 79 54
18
BsiHKAI G_wGCw'C
Cuts at: 4002 4087 5158 4002
Size: 85 1071 4785
Fragments arranged by size:
4785 1071 85
BsmI GAATG_Cn'
Cuts at: 2562 3452 3545 2562
Size: 890 93 4958
Fragments arranged by size:
4958 890 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3179 3179
Size: 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2146 2808 3009 3260 4002 4087 5158
Size: 399 356 662 201 251 742 85 1071
Cuts at: 5158 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 662 399 356 251 201
85
BspEI T'CCGG_A
Cuts at: 2979 5604 2979
Size: 2625 3316
BspHI T'CATG_A
Cuts at: 2605 3830 5560 2605
Size: 1225 1730 2986
Fragments arranged by size:
2986 1730 1225
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3060 3296 844
Size: 2216 236 3489
Fragments arranged by size:
3489 2216 236
BsrBI CCG'CTC
Cuts at: 1394 1823 3828 1394
Size: 429 2005 3507
Fragments arranged by size:
3507 2005 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2751 4436 4610 65
Size: 1183 1503 1685 174 1396
Fragments arranged by size:
1685 1503 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2700 3099 4585 4772 1700
Size: 110 890 399 1486 187 2869
Fragments arranged by size:
2869 1486 890 399 187 110
BsrGI T'GTAC_A
Cuts at: 96 2397 96
Size: 2301 3640
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstYI r'GATC_y
Cuts at: 1972 3271 3638 4023 4040 5492 5578 5590 5936
Size: 1299 367 385 17 1452 86 12 346
Cuts at: 5936 1972
Size: 1977
Fragments arranged by size:
1977 1452 1299 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2695 2695
Size: 5941
BtgI C'CryG_G
Cuts at: 513 1065 2004 2073 513
Size: 552 939 69 4381
Fragments arranged by size:
4381 939 552 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5202
Fragments arranged by size:
5202 683 56
BtsI GCAGTG_nn'
Cuts at: 2717 2872 2921 3457 4251 4271 2717
Size: 155 49 536 794 20 4387
Fragments arranged by size:
4387 794 536 155 49 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DraIII CAC_nnn'GTG
Cuts at: 2512 2512
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2070 2149
Size: 54 1425 50 275 18 186 54 79
Cuts at: 2149 2517 2673 3057 3388 4277 5906 8
Size: 368 156 384 331 889 1629 43
Fragments arranged by size:
1629 1425 889 384 368 331 275 186
156 79 54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 2070 2149 3388 1537
Size: 275 204 54 79 1239 4090
Fragments arranged by size:
4090 1239 275 204 79 54
EarI CTCTTCn'nnn_
Cuts at: 1353 3871 1353
Size: 2518 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4528 4902 5048 387
Size: 1064 138 2939 374 146 1280
Fragments arranged by size:
2939 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2332 2366 2644 2754 4004 4601 5388
Size: 393 700 34 278 110 1250 597 787
Cuts at: 5388 5656 1239
Size: 268 1524
Fragments arranged by size:
1524 1250 787 700 597 393 278 268
110 34
EcoNI CCTnn'n_nnAGG
Cuts at: 2099 2159 2099
Size: 60 5881
EcoO109I rG'GnC_Cy
Cuts at: 2078 2143 2830 2962 3111 2078
Size: 65 687 132 149 4908
Fragments arranged by size:
4908 687 149 132 65
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2095 2185 2212 2326 5088 1678
Size: 417 90 27 114 2762 2531
Fragments arranged by size:
2762 2531 417 114 90 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2344 2376 3127 3159 4582
Size: 32 135 32 772 32 751 32 1423
Cuts at: 4582 4614 4656 4688 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1423 772 751 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3141 3285 3531 669
Size: 988 281 1203 144 246 3079
Fragments arranged by size:
3079 1203 988 281 246 144
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KasI G'GCGC_C
Cuts at: 2091 2208 2091
Size: 117 5824
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5055 5239 1305
Size: 3750 184 2007
Fragments arranged by size:
3750 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2519 3059 10
Size: 54 1425 343 687 540 2892
Fragments arranged by size:
2892 1425 687 540 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2648 3192 3899 4258 4417 518
Size: 648 1482 544 707 359 159 2042
Fragments arranged by size:
2042 1482 707 648 544 359 159
MspA1I CmG'CkG
Cuts at: 1606 2075 2172 2193 2848 3133 3357 4034 5182
Size: 469 97 21 655 285 224 677 1148
Cuts at: 5182 5427 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 655 469 285 245 224
97 21
NaeI GCC'GGC
Cuts at: 2702 2702
Size: 5941
NarI GG'CG_CC
Cuts at: 2092 2209 2092
Size: 117 5824
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4450
Fragments arranged by size:
4450 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NgoMIV G'CCGG_C
Cuts at: 2700 2700
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 2140 3299 4844 1132
Size: 97 911 1159 1545 2229
Fragments arranged by size:
2229 1545 1159 911 97
PasI CC'CwG_GG
Cuts at: 1348 2879 1348
Size: 1531 4410
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2682 2885 3055 3223 1108
Size: 1574 203 170 168 3826
Fragments arranged by size:
3826 1574 203 170 168
PfoI T'CCnGG_A
Cuts at: 3222 3222
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2830 2962 2830
Size: 132 5809
PshAI GACnn'nnGTC
Cuts at: 1613 2273 3102 1613
Size: 660 829 4452
Fragments arranged by size:
4452 829 660
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 2142 2142
Size: 5941
PstI C_TGCA'G
Cuts at: 830 3036 3293 830
Size: 2206 257 3478
Fragments arranged by size:
3478 2206 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2193 2848 2193
Size: 655 5286
SacII CC_GC'GG
Cuts at: 2076 2076
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3139 3283 1936
Size: 1203 144 4594
Fragments arranged by size:
4594 1203 144
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 2597 4189 5901 2597
Size: 1592 1712 2637
Fragments arranged by size:
2637 1712 1592
SfcI C'TryA_G
Cuts at: 826 1045 3032 3289 4424 5105 5296 826
Size: 219 1987 257 1135 681 191 1471
Fragments arranged by size:
1987 1471 1135 681 257 219 191
SfoI GGC'GCC
Cuts at: 2093 2210 2093
Size: 117 5824
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2222 3268 1872
Size: 350 1046 4545
Fragments arranged by size:
4545 1046 350
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4045 4946 5208 820
Size: 197 926 2102 901 262 1553
Fragments arranged by size:
2102 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SrfI GCCC'GGGC
Cuts at: 2222 2222
Size: 5941
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 2004 2676 3380 513
Size: 552 939 672 704 3074
Fragments arranged by size:
3074 939 704 672 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2754 3968 3985 4138 4323 4768 325
Size: 1255 1174 1214 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1214 1174 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2397 2595 3024
Size: 274 80 33 51 1443 420 198 429
Cuts at: 3024 3162 4187 5899 96
Size: 138 1025 1712 138
Fragments arranged by size:
1712 1443 1025 429 420 274 198 138
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5901 5933 5901
Size: 32 5909
Tth111I GACn'n_nGTC
Cuts at: 1316 2741 1316
Size: 1425 4516
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2220 3266 1870
Size: 350 1046 4545
Fragments arranged by size:
4545 1046 350
XmnI GAAnn'nnTTC
Cuts at: 4070 4070
Size: 5941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3138 3749 276
Size: 53 83 186 936 1604 611 2468
Fragments arranged by size:
2468 1604 936 611 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI
SapI SexAI SfiI SwaI XcmI