Restriction map for
pFN21AE4077
(Circular) (Six-base) MAPSORT of: pFN21AE4077.seq Check: 9957 from: 1 to: 5914
pFN21AE4077 5914 bp
With 182 enzymes: SgfI *
March 28, 2012 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2300 2300
Size: 5914
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3724 278
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3257 1937
Size: 1320 4594
Acc65I G'GTAC_C
Cuts at: 3235 3235
Size: 5914
AclI AA'CG_TT
Cuts at: 1563 2901 4041 4414 1563
Size: 1338 1140 373 3063
Fragments arranged by size:
3063 1338 1140 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 3120 3977 5361 5629 1632
Size: 470 1018 857 1384 268 1917
Fragments arranged by size:
1917 1384 1018 857 470 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5717
AflIII A'CryG_T
Cuts at: 1203 4813 5790 1203
Size: 3610 977 1327
Fragments arranged by size:
3610 1327 977
AgeI A'CCGG_T
Cuts at: 4745 4745
Size: 5914
AhdI GACnn_n'nnGTC
Cuts at: 4643 4643
Size: 5914
AleI CACnn'nnGTG
Cuts at: 1166 2167 1166
Size: 1001 4913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5914
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2929 2961 2929
Size: 32 5882
AlwNI CAG_nnn'CTG
Cuts at: 3657 5229 3657
Size: 1572 4342
ApaLI G'TGCA_C
Cuts at: 3971 5127 3971
Size: 1156 4758
ApoI r'AATT_y
Cuts at: 1493 2157 2750 2832 2922 2978 2988 3188 3223
Size: 664 593 82 90 56 10 200 35
Cuts at: 3223 3449 3601 5710 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 664 593 226 200 152 90
82 56 35 10
AseI AT'TA_AT
Cuts at: 160 1032 2646 2708 3014 4468 160
Size: 872 1614 62 306 1454 1606
Fragments arranged by size:
1614 1606 1454 872 306 62
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
AvaI C'yCGr_G
Cuts at: 1870 1943 2841 3239 1870
Size: 73 898 398 4545
Fragments arranged by size:
4545 898 398 73
BamHI G'GATC_C
Cuts at: 3244 3611 3244
Size: 367 5547
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3235 4690 618
Size: 325 217 627 1448 1455 1842
Fragments arranged by size:
1842 1455 1448 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3233 1391
Size: 1842 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2277 928
Size: 971 378 4565
Fragments arranged by size:
4565 971 378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4105 4139 1903
Size: 34 50 34 2084 34 3678
Fragments arranged by size:
3678 2084 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3806 5016 3806
Size: 1210 4704
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5914
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4525 136
Size: 107 122 71 631 605 2853 1525
Fragments arranged by size:
2853 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2479 2790 5909 2479
Size: 311 3119 2484
Fragments arranged by size:
3119 2484 311
BlpI GC'TnA_GC
Cuts at: 3331 3331
Size: 5914
Bme1580I G_kGCm'C
Cuts at: 1790 2174 3975 5131 1790
Size: 384 1801 1156 2573
Fragments arranged by size:
2573 1801 1156 384
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5914
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3645 4603 442
Size: 416 53 83 399 24 2228 958 1753
Fragments arranged by size:
2228 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2546 4574 1239
Size: 1307 2028 2579
Fragments arranged by size:
2579 2028 1307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2150 4039 4904 5202 2150
Size: 1889 865 298 2862
Fragments arranged by size:
2862 1889 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2140 4577 882
Size: 617 641 2437 2219
Fragments arranged by size:
2437 2219 641 617
BsaAI yAC'GTr
Cuts at: 493 1204 3705 493
Size: 711 2501 2702
Fragments arranged by size:
2702 2501 711
BsaBI GATnn'nnATC
Cuts at: 5908 5908
Size: 5914
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3721 4103 4738 275
Size: 53 83 186 936 2188 382 635 1451
Fragments arranged by size:
2188 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4347 4745 5019 5166 5577 4347
Size: 398 274 147 411 4684
Fragments arranged by size:
4684 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5689
Fragments arranged by size:
5689 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2427 1186
Size: 1241 4673
BseYI C'CCAG_C
Cuts at: 5117 5613 5117
Size: 496 5418
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5341
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3364 4125 5153 1540
Size: 275 186 1363 761 1028 2301
Fragments arranged by size:
2301 1363 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3975 4060 5131 3975
Size: 85 1071 4758
Fragments arranged by size:
4758 1071 85
BsmI GAATG_Cn'
Cuts at: 3425 3518 3425
Size: 93 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 3233 3975 4060 5131 1391
Size: 399 384 1059 742 85 1071 2174
Fragments arranged by size:
2174 1071 1059 742 399 384 85
BspEI T'CCGG_A
Cuts at: 5577 5577
Size: 5914
BspHI T'CATG_A
Cuts at: 3803 5533 3803
Size: 1730 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2300 3269 844
Size: 1456 969 3489
Fragments arranged by size:
3489 1456 969
BsrBI CCG'CTC
Cuts at: 1394 1823 3801 1394
Size: 429 1978 3507
Fragments arranged by size:
3507 1978 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2359 4409 4583 65
Size: 1183 1111 2050 174 1396
Fragments arranged by size:
2050 1396 1183 1111 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4558 4745 1700
Size: 110 2748 187 2869
Fragments arranged by size:
2869 2748 187 110
BsrGI T'GTAC_A
Cuts at: 96 2785 96
Size: 2689 3225
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5914
BssSI C'ACGA_G
Cuts at: 2064 3974 4986 2064
Size: 1910 1012 2992
Fragments arranged by size:
2992 1910 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5914
BstBI TT'CG_AA
Cuts at: 4718 4718
Size: 5914
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5914
BstYI r'GATC_y
Cuts at: 1972 2059 2479 2790 3244 3611 3996 4013 5465
Size: 87 420 311 454 367 385 17 1452
Cuts at: 5465 5551 5563 5909 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 454 420 385 367 346 311
87 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2814 2814
Size: 5914
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5175
Fragments arranged by size:
5175 683 56
BtsI GCAGTG_nn'
Cuts at: 3430 4224 4244 3430
Size: 794 20 5100
Fragments arranged by size:
5100 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5914
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5879
DraI TTT'AAA
Cuts at: 2985 2994 3218 2985
Size: 9 224 5681
Fragments arranged by size:
5681 224 9
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4921 809
Size: 507 3605 1802
Fragments arranged by size:
3605 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3361 4250 5879
Size: 54 1425 50 275 18 1531 889 1629
Cuts at: 5879 8
Size: 43
Fragments arranged by size:
1629 1531 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3361 1537
Size: 275 1549 4090
Fragments arranged by size:
4090 1549 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2016 2730 3844 1353
Size: 663 714 1114 3423
Fragments arranged by size:
3423 1114 714 663
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4501 4875 5021 387
Size: 1064 138 2912 374 146 1280
Fragments arranged by size:
2912 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2546 3120 3977 4574 5361 5629
Size: 393 470 444 574 857 597 787 268
Cuts at: 5629 1239
Size: 1524
Fragments arranged by size:
1524 857 787 597 574 470 444 393
268
EcoRI G'AATT_C
Cuts at: 2157 3223 3601 2157
Size: 1066 378 4470
Fragments arranged by size:
4470 1066 378
FspI TGC'GCA
Cuts at: 4420 4420
Size: 5914
HaeII r_GCGC'y
Cuts at: 1678 5061 1678
Size: 3383 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4555 4587 4629 4661 1373
Size: 32 135 32 2983 32 42 32 2626
Fragments arranged by size:
2983 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2028 2378 2460 3258 3504 669
Size: 988 281 90 350 82 798 246 3079
Fragments arranged by size:
3079 988 798 350 281 246 90 82
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5914
HpaI GTT'AAC
Cuts at: 2028 3504 2028
Size: 1476 4438
KpnI G_GTAC'C
Cuts at: 3239 3239
Size: 5914
MfeI C'AATT_G
Cuts at: 3513 3513
Size: 5914
MluI A'CGCG_T
Cuts at: 5790 5790
Size: 5914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2228 5028 5212 1305
Size: 923 2800 184 2007
Fragments arranged by size:
2800 2007 923 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4092
Fragments arranged by size:
4092 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2167 3872 4231 4390 518
Size: 648 1001 1705 359 159 2042
Fragments arranged by size:
2042 1705 1001 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3330 4007 5155 5400 1606
Size: 1724 677 1148 245 2120
Fragments arranged by size:
2120 1724 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5362
NdeI CA'TA_TG
Cuts at: 387 2039 387
Size: 1652 4262
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5914
NotI GC'GGCC_GC
Cuts at: 3361 3361
Size: 5914
NspI r_CATG'y
Cuts at: 1132 1229 2786 3272 4817 1132
Size: 97 1557 486 1545 2229
Fragments arranged by size:
2229 1557 1545 486 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5914
PciI A'CATG_T
Cuts at: 4813 4813
Size: 5914
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5914
PmeI GTTT'AAAC
Cuts at: 3218 3218
Size: 5914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 5496 5528 3983
Size: 32 1481 32 4369
Fragments arranged by size:
4369 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5914
PsiI TTA'TAA
Cuts at: 3484 3484
Size: 5914
PspXI vC'TCGA_Gb
Cuts at: 2841 2841
Size: 5914
PstI C_TGCA'G
Cuts at: 830 3266 830
Size: 2436 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5914
SalI G'TCGA_C
Cuts at: 1936 3256 1936
Size: 1320 4594
SbfI CC_TGCA'GG
Cuts at: 3266 3266
Size: 5914
ScaI AGT'ACT
Cuts at: 4162 5874 4162
Size: 1712 4202
SfcI C'TryA_G
Cuts at: 826 1045 2394 3262 4397 5078 5269 826
Size: 219 1349 868 1135 681 191 1471
Fragments arranged by size:
1471 1349 1135 868 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5914
SmaI CCC'GGG
Cuts at: 1872 3241 1872
Size: 1369 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2165 2841 4018 4919 5181 820
Size: 197 926 222 676 1177 901 262 1553
Fragments arranged by size:
1553 1177 926 901 676 262 222 197
SnaBI TAC'GTA
Cuts at: 493 3705 493
Size: 3212 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5914
SphI G_CATG'C
Cuts at: 1132 3272 1132
Size: 2140 3774
SspI AAT'ATT
Cuts at: 5 52 2856 3838 5
Size: 47 2804 982 2081
Fragments arranged by size:
2804 2081 982 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5914
StyI C'CwwG_G
Cuts at: 513 1065 2539 3353 513
Size: 552 1474 814 3074
Fragments arranged by size:
3074 1474 814 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3941 3958 4111 4296 4741 325
Size: 1255 2361 17 153 185 445 1498
Fragments arranged by size:
2361 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2068 2785 4160
Size: 274 80 33 51 1443 91 717 1375
Cuts at: 4160 5872 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1375 717 274 138 91 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3179 3211 5874 5906 3179
Size: 32 2663 32 3187
Fragments arranged by size:
3187 2663 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5914
XbaI T'CTAG_A
Cuts at: 3250 3250
Size: 5914
XhoI C'TCGA_G
Cuts at: 1943 2841 1943
Size: 898 5016
XmaI C'CCGG_G
Cuts at: 1870 3239 1870
Size: 1369 4545
XmnI GAAnn'nnTTC
Cuts at: 2112 2556 4043 2112
Size: 444 1487 3983
Fragments arranged by size:
3983 1487 444
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3722 276
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI