Restriction map for
pFN21AE4072
(Circular) (Six-base) MAPSORT of: pFN21AE4072.seq Check: 8452 from: 1 to: 5842
pFN21AE4072 5842 bp
With 182 enzymes: SgfI *
March 28, 2012 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2569 2569
Size: 5842
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2334 3652 278
Size: 53 83 186 936 798 1318 2468
Fragments arranged by size:
2468 1318 936 798 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3185 1937
Size: 1248 4594
Acc65I G'GTAC_C
Cuts at: 3163 3163
Size: 5842
AclI AA'CG_TT
Cuts at: 1563 3969 4342 1563
Size: 2406 373 3063
Fragments arranged by size:
3063 2406 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 2926 3905 5289 5557 1632
Size: 653 641 979 1384 268 1917
Fragments arranged by size:
1917 1384 979 653 641 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5645
AflIII A'CryG_T
Cuts at: 1203 4741 5718 1203
Size: 3538 977 1327
Fragments arranged by size:
3538 1327 977
AgeI A'CCGG_T
Cuts at: 4673 4673
Size: 5842
AhdI GACnn_n'nnGTC
Cuts at: 4571 4571
Size: 5842
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5842
AlwNI CAG_nnn'CTG
Cuts at: 3585 5157 3585
Size: 1572 4270
ApaI G_GGCC'C
Cuts at: 2177 2222 2254 2177
Size: 45 32 5765
Fragments arranged by size:
5765 45 32
ApaLI G'TGCA_C
Cuts at: 2406 3899 5055 2406
Size: 1493 1156 3193
Fragments arranged by size:
3193 1493 1156
ApoI r'AATT_y
Cuts at: 1493 2105 3113 3151 3377 3529 5638 1493
Size: 612 1008 38 226 152 2109 1697
Fragments arranged by size:
2109 1697 1008 612 226 152 38
AseI AT'TA_AT
Cuts at: 160 1032 4396 160
Size: 872 3364 1606
Fragments arranged by size:
3364 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
AvaI C'yCGr_G
Cuts at: 1870 1943 2157 2176 2442 2488 2495 2591 2663
Size: 73 214 19 266 46 7 96 72
Cuts at: 2663 2847 3167 1870
Size: 184 320 4545
Fragments arranged by size:
4545 320 266 214 184 96 73 72
46 19 7
BamHI G'GATC_C
Cuts at: 3172 3539 3172
Size: 367 5475
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2278 2599 2878 3163 4618
Size: 325 217 627 491 321 279 285 1455
Cuts at: 4618 618
Size: 1842
Fragments arranged by size:
1842 1455 627 491 325 321 285 279
217
BanII G_rGCy'C
Cuts at: 1391 2177 2222 2254 2583 3102 3161 1391
Size: 786 45 32 329 519 59 4072
Fragments arranged by size:
4072 786 519 329 59 45 32
BbeI G_GCGC'C
Cuts at: 2603 2882 2603
Size: 279 5563
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2997 928
Size: 971 1098 3773
Fragments arranged by size:
3773 1098 971
BbvCI CC'TCA_GC
Cuts at: 2502 2502
Size: 5842
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4033 4067 1903
Size: 34 2096 34 3678
Fragments arranged by size:
3678 2096 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3734 4944 3734
Size: 1210 4632
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5842
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2244 2666 2699
Size: 107 122 71 631 605 572 422 33
Cuts at: 2699 2824 4453 136
Size: 125 1629 1525
Fragments arranged by size:
1629 1525 631 605 572 422 125 122
107 71 33
BglII A'GATC_T
Cuts at: 2719 5837 2719
Size: 3118 2724
BlpI GC'TnA_GC
Cuts at: 3259 3259
Size: 5842
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2131 2177 2222 2254 2281 2345 2410
Size: 231 110 46 45 32 27 64 65
Cuts at: 2410 3903 5059 1790
Size: 1493 1156 2573
Fragments arranged by size:
2573 1493 1156 231 110 65 64 46
45 32 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5842
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2201 2532 3573
Size: 416 53 83 399 24 784 331 1041
Cuts at: 3573 4531 442
Size: 958 1753
Fragments arranged by size:
1753 1041 958 784 416 399 331 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2655 3011 4502 1239
Size: 1416 356 1491 2579
Fragments arranged by size:
2579 1491 1416 356
Bpu10I CC'TnA_GC
Cuts at: 2502 3096 2502
Size: 594 5248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3967 4832 5130 3967
Size: 865 298 4679
Fragments arranged by size:
4679 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 2498 2991 4505 882
Size: 617 504 495 493 1514 2219
Fragments arranged by size:
2219 1514 617 504 495 493
BsaAI yAC'GTr
Cuts at: 493 1204 3633 493
Size: 711 2429 2702
Fragments arranged by size:
2702 2429 711
BsaBI GATnn'nnATC
Cuts at: 5836 5836
Size: 5842
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 2162 2331 2600
Size: 53 83 186 936 512 117 169 269
Cuts at: 2600 2879 3649 4031 4666 275
Size: 279 770 382 635 1451
Fragments arranged by size:
1451 936 770 635 512 382 279 269
186 169 117 83 53
BsaWI w'CCGG_w
Cuts at: 4275 4673 4947 5094 5505 4275
Size: 398 274 147 411 4612
Fragments arranged by size:
4612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2907 2937 1178
Size: 30 165 30 597 30 877 30 4083
Fragments arranged by size:
4083 877 597 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2372 2939 1186
Size: 1186 567 4089
Fragments arranged by size:
4089 1186 567
BseYI C'CCAG_C
Cuts at: 2247 2512 5045 5541 2247
Size: 265 2533 496 2548
Fragments arranged by size:
2548 2533 496 265
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2898 1163
Size: 573 1162 4107
Fragments arranged by size:
4107 1162 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2286 2609 2658 2709 2743 2759
Size: 275 186 285 323 49 51 34 16
Cuts at: 2759 2828 3292 4053 5081 1540
Size: 69 464 761 1028 2301
Fragments arranged by size:
2301 1028 761 464 323 285 275 186
69 51 49 34 16
BsiHKAI G_wGCw'C
Cuts at: 2410 2939 3903 3988 5059 2410
Size: 529 964 85 1071 3193
Fragments arranged by size:
3193 1071 964 529 85
BsmI GAATG_Cn'
Cuts at: 3353 3446 3353
Size: 93 5749
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2131 2177 2222 2254 2281 2345
Size: 399 231 110 46 45 32 27 64
Cuts at: 2345 2410 2583 2939 3102 3161 3903 3988 5059
Size: 65 173 356 163 59 742 85 1071
Cuts at: 5059 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 356 231 173 163
110 85 65 64 59 46 45 32
27
BspEI T'CCGG_A
Cuts at: 5505 5505
Size: 5842
BspHI T'CATG_A
Cuts at: 3731 5461 3731
Size: 1730 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2569 3197 844
Size: 1725 628 3489
Fragments arranged by size:
3489 1725 628
BsrBI CCG'CTC
Cuts at: 1394 1823 2824 3729 1394
Size: 429 1001 905 3507
Fragments arranged by size:
3507 1001 905 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4337 4511 65
Size: 1183 3089 174 1396
Fragments arranged by size:
3089 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2041 2315 2653 2754 2842 2868 2875
Size: 110 231 274 338 101 88 26 7
Cuts at: 2875 3007 4486 4673 1700
Size: 132 1479 187 2869
Fragments arranged by size:
2869 1479 338 274 231 187 132 110
101 88 26 7
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5842
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5842
BssSI C'ACGA_G
Cuts at: 2932 3902 4914 2932
Size: 970 1012 3860
Fragments arranged by size:
3860 1012 970
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5842
BstBI TT'CG_AA
Cuts at: 4646 4646
Size: 5842
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5842
BstYI r'GATC_y
Cuts at: 1972 2719 3172 3539 3924 3941 5393 5479 5491
Size: 747 453 367 385 17 1452 86 12
Cuts at: 5491 5837 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 747 453 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2223 2529 2223
Size: 306 5536
BtgI C'CryG_G
Cuts at: 513 1065 2004 2350 2583 2603 513
Size: 552 939 346 233 20 3752
Fragments arranged by size:
3752 939 552 346 233 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5103
Fragments arranged by size:
5103 683 56
BtsI GCAGTG_nn'
Cuts at: 2043 3358 4152 4172 2043
Size: 1315 794 20 3713
Fragments arranged by size:
3713 1315 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5807
DraI TTT'AAA
Cuts at: 3146 3146
Size: 5842
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4849 809
Size: 507 3533 1802
Fragments arranged by size:
3533 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2606 2620 2655
Size: 54 1425 50 275 18 776 14 35
Cuts at: 2655 2676 2691 2706 2825 3289 4178 5807 8
Size: 21 15 15 119 464 889 1629 43
Fragments arranged by size:
1629 1425 889 776 464 275 119 54
50 43 35 21 18 15 15 14
EagI C'GGCC_G
Cuts at: 1537 1812 2606 2655 2706 2825 3289 1537
Size: 275 794 49 51 119 464 4090
Fragments arranged by size:
4090 794 464 275 119 51 49
EarI CTCTTCn'nnn_
Cuts at: 1353 2358 3772 1353
Size: 1005 1414 3423
Fragments arranged by size:
3423 1414 1005
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2385 2714 2741 4429 4803 4949
Size: 1064 138 796 329 27 1688 374 146
Cuts at: 4949 387
Size: 1280
Fragments arranged by size:
1688 1280 1064 796 374 329 146 138
27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2655 2926 3011 3905 4502 5289
Size: 393 653 370 271 85 894 597 787
Cuts at: 5289 5557 1239
Size: 268 1524
Fragments arranged by size:
1524 894 787 653 597 393 370 271
268 85
EcoNI CCTnn'n_nnAGG
Cuts at: 2835 2835
Size: 5842
EcoO109I rG'GnC_Cy
Cuts at: 2097 2180 2218 2219 2227 2251 2588 3083 2097
Size: 83 38 1 8 24 337 495 4856
Fragments arranged by size:
4856 495 337 83 38 24 8 1
EcoRI G'AATT_C
Cuts at: 2105 3151 3529 2105
Size: 1046 378 4418
Fragments arranged by size:
4418 1046 378
FspI TGC'GCA
Cuts at: 4348 4348
Size: 5842
HaeII r_GCGC'y
Cuts at: 1678 2603 2882 4989 1678
Size: 925 279 2107 2531
Fragments arranged by size:
2531 2107 925 279
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3067 3099 4483 4515 4557
Size: 32 135 32 1495 32 1384 32 42
Cuts at: 4557 4589 1373
Size: 32 2626
Fragments arranged by size:
2626 1495 1384 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3186 3432 669
Size: 988 281 1248 246 3079
Fragments arranged by size:
3079 1248 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5842
HpaI GTT'AAC
Cuts at: 3432 3432
Size: 5842
KasI G'GCGC_C
Cuts at: 2599 2878 2599
Size: 279 5563
KpnI G_GTAC'C
Cuts at: 3167 3167
Size: 5842
MfeI C'AATT_G
Cuts at: 3441 3441
Size: 5842
MluI A'CGCG_T
Cuts at: 5718 5718
Size: 5842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4956 5140 1305
Size: 3651 184 2007
Fragments arranged by size:
3651 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4020
Fragments arranged by size:
4020 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3800 4159 4318 518
Size: 648 2634 359 159 2042
Fragments arranged by size:
2634 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2116 2352 2421 2585 2605 2675 3061 3089
Size: 510 236 69 164 20 70 386 28
Cuts at: 3089 3258 3935 5083 5328 1606
Size: 169 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 510 386 245 236 169
164 70 69 28 20
NaeI GCC'GGC
Cuts at: 2043 2317 2655 2844 2870 2877 3009 2043
Size: 274 338 189 26 7 132 4876
Fragments arranged by size:
4876 338 274 189 132 26 7
NarI GG'CG_CC
Cuts at: 2600 2879 2600
Size: 279 5563
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4351
Fragments arranged by size:
4351 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5842
NgoMIV G'CCGG_C
Cuts at: 2041 2315 2653 2842 2868 2875 3007 2041
Size: 274 338 189 26 7 132 4876
Fragments arranged by size:
4876 338 274 189 132 26 7
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5842
NotI GC'GGCC_GC
Cuts at: 2825 3289 2825
Size: 464 5378
NspI r_CATG'y
Cuts at: 1132 1229 3200 4745 1132
Size: 97 1971 1545 2229
Fragments arranged by size:
2229 1971 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5842
PciI A'CATG_T
Cuts at: 4741 4741
Size: 5842
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5842
PmeI GTTT'AAAC
Cuts at: 3146 3146
Size: 5842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 5424 5456 3911
Size: 32 1481 32 4297
Fragments arranged by size:
4297 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2097 2588 3083 2097
Size: 491 495 4856
Fragments arranged by size:
4856 495 491
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5842
PsiI TTA'TAA
Cuts at: 3412 3412
Size: 5842
PspOMI G'GGCC_C
Cuts at: 2173 2218 2250 2173
Size: 45 32 5765
Fragments arranged by size:
5765 45 32
PstI C_TGCA'G
Cuts at: 830 3194 830
Size: 2364 3478
PvuI CG_AT'CG
Cuts at: 2001 2743 2001
Size: 742 5100
SacII CC_GC'GG
Cuts at: 2353 2586 2606 2353
Size: 233 20 5589
Fragments arranged by size:
5589 233 20
SalI G'TCGA_C
Cuts at: 1936 3184 1936
Size: 1248 4594
SanDI GG'GwC_CC
Cuts at: 2097 2588 3083 2097
Size: 491 495 4856
Fragments arranged by size:
4856 495 491
SbfI CC_TGCA'GG
Cuts at: 3194 3194
Size: 5842
ScaI AGT'ACT
Cuts at: 4090 5802 4090
Size: 1712 4130
SfcI C'TryA_G
Cuts at: 826 1045 3190 4325 5006 5197 826
Size: 219 2145 1135 681 191 1471
Fragments arranged by size:
2145 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2824 2824
Size: 5842
SfoI GGC'GCC
Cuts at: 2601 2880 2601
Size: 279 5563
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
SgrAI Cr'CCGG_yG
Cuts at: 1700 2315 1700
Size: 615 5227
SmaI CCC'GGG
Cuts at: 1872 2159 2178 2490 2593 3169 1872
Size: 287 19 312 103 576 4545
Fragments arranged by size:
4545 576 312 287 103 19
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2495 3946 4847 5109 820
Size: 197 926 552 1451 901 262 1553
Fragments arranged by size:
1553 1451 926 901 552 262 197
SnaBI TAC'GTA
Cuts at: 493 3633 493
Size: 3140 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5842
SphI G_CATG'C
Cuts at: 1132 3200 1132
Size: 2068 3774
SspI AAT'ATT
Cuts at: 5 52 3766 5
Size: 47 3714 2081
Fragments arranged by size:
3714 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5842
StyI C'CwwG_G
Cuts at: 513 1065 2004 2790 3281 513
Size: 552 939 786 491 3074
Fragments arranged by size:
3074 939 786 552 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3869 3886 4039 4224 4669 325
Size: 1255 2289 17 153 185 445 1498
Fragments arranged by size:
2289 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4088 5800 96
Size: 274 80 33 51 1443 2111 1712 138
Fragments arranged by size:
2111 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2907 2939 5802 5834 2000
Size: 32 875 32 2863 32 2008
Fragments arranged by size:
2863 2008 875 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5842
XbaI T'CTAG_A
Cuts at: 3178 3178
Size: 5842
XhoI C'TCGA_G
Cuts at: 1943 2495 1943
Size: 552 5290
XmaI C'CCGG_G
Cuts at: 1870 2157 2176 2488 2591 3167 1870
Size: 287 19 312 103 576 4545
Fragments arranged by size:
4545 576 312 287 103 19
XmnI GAAnn'nnTTC
Cuts at: 2783 3971 2783
Size: 1188 4654
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2332 3650 276
Size: 53 83 186 936 798 1318 2468
Fragments arranged by size:
2468 1318 936 798 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacII SalI SanDI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacI SapI SexAI SrfI SwaI XcmI