Restriction map for
pFN21AE4068
(Circular) (Six-base) MAPSORT of: pFN21AE4068.seq Check: 5756 from: 1 to: 5770
pFN21AE4068 5770 bp
With 182 enzymes: SgfI *
March 28, 2012 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2914 2914
Size: 5770
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2516 3580 278
Size: 53 83 186 936 980 1064 2468
Fragments arranged by size:
2468 1064 980 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2481 3113 1937
Size: 544 632 4594
Fragments arranged by size:
4594 632 544
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3833 5217 5485 1632
Size: 2201 1384 268 1917
Fragments arranged by size:
2201 1917 1384 268
AfeI AGC'GCT
Cuts at: 3056 3056
Size: 5770
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 2544 4669 5646 1203
Size: 1341 2125 977 1327
Fragments arranged by size:
2125 1341 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5770
AlwNI CAG_nnn'CTG
Cuts at: 2034 2283 2842 3513 5085 2034
Size: 249 559 671 1572 2719
Fragments arranged by size:
2719 1572 671 559 249
ApaLI G'TGCA_C
Cuts at: 2669 3827 4983 2669
Size: 1158 1156 3456
Fragments arranged by size:
3456 1158 1156
ApoI r'AATT_y
Cuts at: 1493 3079 3305 3457 5566 1493
Size: 1586 226 152 2109 1697
Fragments arranged by size:
2109 1697 1586 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 2153 2878 3095 1870
Size: 73 133 77 725 217 4545
Fragments arranged by size:
4545 725 217 133 77 73
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2191 2423 2486 3091 4546
Size: 325 217 627 404 232 63 605 1455
Cuts at: 4546 618
Size: 1842
Fragments arranged by size:
1842 1455 627 605 404 325 232 217
63
BanII G_rGCy'C
Cuts at: 1391 2657 3089 1391
Size: 1266 432 4072
Fragments arranged by size:
4072 1266 432
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2307 928
Size: 971 408 4391
Fragments arranged by size:
4391 971 408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3961 3995 1903
Size: 34 2024 34 3678
Fragments arranged by size:
3678 2024 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3662 4872 3662
Size: 1210 4560
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2562 4381 136
Size: 107 122 71 631 605 890 1819 1525
Fragments arranged by size:
1819 1525 890 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5765 5765
Size: 5770
BlpI GC'TnA_GC
Cuts at: 2357 3187 2357
Size: 830 4940
Bme1580I G_kGCm'C
Cuts at: 1790 2196 2426 2673 3831 4987 1790
Size: 406 230 247 1158 1156 2573
Fragments arranged by size:
2573 1158 1156 406 247 230
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2116 2178 2326
Size: 416 53 83 399 24 699 62 148
Cuts at: 2326 3501 4459 442
Size: 1175 958 1753
Fragments arranged by size:
1753 1175 958 699 416 399 148 83
62 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2819 2912 2967 4430 1239
Size: 1580 93 55 1463 2579
Fragments arranged by size:
2579 1580 1463 93 55
Bpu10I CC'TnA_GC
Cuts at: 2954 2954
Size: 5770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2903 3895 4760 5058 2903
Size: 992 865 298 3615
Fragments arranged by size:
3615 992 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4433 882
Size: 617 2934 2219
Fragments arranged by size:
2934 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3561 493
Size: 711 2357 2702
Fragments arranged by size:
2702 2357 711
BsaBI GATnn'nnATC
Cuts at: 5764 5764
Size: 5770
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2014 2513 3577 3959
Size: 53 83 186 936 481 499 1064 382
Cuts at: 3959 4594 275
Size: 635 1451
Fragments arranged by size:
1451 1064 936 635 499 481 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4203 4601 4875 5022 5433 4203
Size: 398 274 147 411 4540
Fragments arranged by size:
4540 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2129 2159 1178
Size: 30 165 30 726 30 4789
Fragments arranged by size:
4789 726 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2594 1186
Size: 1408 4362
BseYI C'CCAG_C
Cuts at: 2225 2434 2560 2580 2941 2946 3020 4973 5469
Size: 209 126 20 361 5 74 1953 496
Cuts at: 5469 2225
Size: 2526
Fragments arranged by size:
2526 1953 496 361 209 126 74 20
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2073 2243 1163
Size: 573 337 170 4690
Fragments arranged by size:
4690 573 337 170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3220 3981 5009 1540
Size: 275 186 1219 761 1028 2301
Fragments arranged by size:
2301 1219 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2673 2777 3831 3916 4987 2673
Size: 104 1054 85 1071 3456
Fragments arranged by size:
3456 1071 1054 104 85
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
BsmBI CGTCTCn'nnnn_
Cuts at: 2058 2058
Size: 5770
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2426 2657 2673 2777 3089 3831
Size: 399 406 230 231 16 104 312 742
Cuts at: 3831 3916 4987 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 406 399 312 231 230
104 85 16
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 3659 5389 3659
Size: 1730 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2914 3125 844
Size: 2070 211 3489
Fragments arranged by size:
3489 2070 211
BsrBI CCG'CTC
Cuts at: 1394 1823 2811 3657 1394
Size: 429 988 846 3507
Fragments arranged by size:
3507 988 846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4265 4439 65
Size: 1183 3017 174 1396
Fragments arranged by size:
3017 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2201 4414 4601 1700
Size: 110 391 2213 187 2869
Fragments arranged by size:
2869 2213 391 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5770
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2283 2964 794
Size: 1489 681 3600
Fragments arranged by size:
3600 1489 681
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 2803 1184
Size: 1619 4151
BstXI CCAn_nnnn'nTGG
Cuts at: 2434 2434
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 3100 3467 3852 3869 5321 5407 5419 5765
Size: 1128 367 385 17 1452 86 12 346
Cuts at: 5765 1972
Size: 1977
Fragments arranged by size:
1977 1452 1128 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2082 2778 513
Size: 552 939 78 696 3505
Fragments arranged by size:
3505 939 696 552 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5031
Fragments arranged by size:
5031 683 56
BtsI GCAGTG_nn'
Cuts at: 3286 4080 4100 3286
Size: 794 20 4956
Fragments arranged by size:
4956 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2206 4777 809
Size: 507 890 2571 1802
Fragments arranged by size:
2571 1802 890 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2412 2647
Size: 54 1425 50 275 18 177 405 235
Cuts at: 2647 2870 3217 4106 5735 8
Size: 223 347 889 1629 43
Fragments arranged by size:
1629 1425 889 405 347 275 235 223
177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3700 1353
Size: 2347 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3001 4357 4731 4877 387
Size: 1064 138 1412 1356 374 146 1280
Fragments arranged by size:
1412 1356 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2819 2912 2967 3833 4430 5217 5485
Size: 393 1187 93 55 866 597 787 268
Cuts at: 5485 1239
Size: 1524
Fragments arranged by size:
1524 1187 866 787 597 393 268 93
55
EcoNI CCTnn'n_nnAGG
Cuts at: 2921 3046 2921
Size: 125 5645
EcoO109I rG'GnC_Cy
Cuts at: 2031 2584 2950 2031
Size: 553 366 4851
Fragments arranged by size:
4851 553 366
EcoRI G'AATT_C
Cuts at: 3079 3457 3079
Size: 378 5392
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2647 2398
Size: 249 5521
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 2826 3058 4917 1678
Size: 1148 232 1859 2531
Fragments arranged by size:
2531 1859 1148 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 2458 2490 4411
Size: 32 135 32 557 32 297 32 1921
Cuts at: 4411 4443 4485 4517 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1921 557 297 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3360 669
Size: 988 281 1176 246 3079
Fragments arranged by size:
3079 1176 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2172 2496 4884 5068 1305
Size: 867 324 2388 184 2007
Fragments arranged by size:
2388 2007 867 324 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3771
Fragments arranged by size:
3771 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 2498 2666 3728 4087 4246 518
Size: 648 1326 6 168 1062 359 159 2042
Fragments arranged by size:
2042 1326 1062 648 359 168 159 6
MspA1I CmG'CkG
Cuts at: 1606 2289 2400 2411 2580 2866 2905 3186 3863
Size: 683 111 11 169 286 39 281 677
Cuts at: 3863 5011 5256 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 683 677 286 281 245 169
111 39 11
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4279
Fragments arranged by size:
4279 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NruI TCG'CGA
Cuts at: 3030 3030
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 3128 4673 1132
Size: 97 1899 1545 2229
Fragments arranged by size:
2229 1899 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5770
PciI A'CATG_T
Cuts at: 4669 4669
Size: 5770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2106 1108
Size: 998 4772
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2950 2950
Size: 5770
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PstI C_TGCA'G
Cuts at: 830 2555 2748 3122 830
Size: 1725 193 374 3478
Fragments arranged by size:
3478 1725 374 193
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
PvuII CAG'CTG
Cuts at: 2400 2580 2866 2400
Size: 180 286 5304
Fragments arranged by size:
5304 286 180
RsrII CG'GwC_CG
Cuts at: 3066 3066
Size: 5770
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 2159 4018 5730 2159
Size: 1859 1712 2199
Fragments arranged by size:
2199 1859 1712
SfcI C'TryA_G
Cuts at: 826 1045 2248 2551 2744 3118 4253 4934 5125
Size: 219 1203 303 193 374 1135 681 191
Cuts at: 5125 826
Size: 1471
Fragments arranged by size:
1471 1203 1135 681 374 303 219 193
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 2078 3097 1872
Size: 206 1019 4545
Fragments arranged by size:
4545 1019 206
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2918 3874 4775 5037 820
Size: 197 926 975 956 901 262 1553
Fragments arranged by size:
1553 975 956 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SrfI GCCC'GGGC
Cuts at: 2078 2078
Size: 5770
SspI AAT'ATT
Cuts at: 5 52 3694 5
Size: 47 3642 2081
Fragments arranged by size:
3642 2081 47
StuI AGG'CCT
Cuts at: 1517 2240 1517
Size: 723 5047
StyI C'CwwG_G
Cuts at: 513 1065 2004 2427 3209 513
Size: 552 939 423 782 3074
Fragments arranged by size:
3074 939 782 552 423
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3797 3814 3967 4152 4597 325
Size: 1255 2217 17 153 185 445 1498
Fragments arranged by size:
2217 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2157 2394 4016
Size: 274 80 33 51 1443 180 237 1622
Cuts at: 4016 5728 96
Size: 1712 138
Fragments arranged by size:
1712 1622 1443 274 237 180 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5730 5762 5730
Size: 32 5738
Tth111I GACn'n_nGTC
Cuts at: 1316 2318 1316
Size: 1002 4768
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 2076 3095 1870
Size: 206 1019 4545
Fragments arranged by size:
4545 1019 206
XmnI GAAnn'nnTTC
Cuts at: 3899 3899
Size: 5770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2514 3578 276
Size: 53 83 186 936 980 1064 2468
Fragments arranged by size:
2468 1064 980 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NruI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI
PmlI PspOMI PspXI PsrI SacI SacII SanDI SapI
SexAI SfiI SfoI SwaI XcmI