Restriction map for
pFN21AE4065
(Circular) (Six-base) MAPSORT of: pFN21AE4065.seq Check: 1084 from: 1 to: 5743
pFN21AE4065 5743 bp
With 182 enzymes: SgfI *
March 28, 2012 13:09 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2283 3553 278
Size: 53 83 186 936 747 1270 2468
Fragments arranged by size:
2468 1270 936 747 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 1937
Size: 1149 4594
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3806 5190 5458 1632
Size: 2174 1384 268 1917
Fragments arranged by size:
2174 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2248 2311 2344 3486 5058 2248
Size: 63 33 1142 1572 2933
Fragments arranged by size:
2933 1572 1142 63 33
ApaI G_GGCC'C
Cuts at: 2129 2129
Size: 5743
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 3052 3278 3430 5539 1493
Size: 1559 226 152 2109 1697
Fragments arranged by size:
2109 1697 1559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2738 2763 3068 1870
Size: 73 795 25 305 4545
Fragments arranged by size:
4545 795 305 73 25
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2231 3064 4519 618
Size: 325 217 627 279 165 833 1455 1842
Fragments arranged by size:
1842 1455 833 627 325 279 217 165
BanII G_rGCy'C
Cuts at: 1391 2129 3062 1391
Size: 738 933 4072
Fragments arranged by size:
4072 933 738
BbeI G_GCGC'C
Cuts at: 2235 2235
Size: 5743
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2830 928
Size: 971 931 3841
Fragments arranged by size:
3841 971 931
BbvCI CC'TCA_GC
Cuts at: 2195 2457 2475 2547 2652 2195
Size: 262 18 72 105 5286
Fragments arranged by size:
5286 262 105 72 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BfrBI ATG'CAT
Cuts at: 2944 2944
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2207 4354 136
Size: 107 122 71 631 605 535 2147 1525
Fragments arranged by size:
2147 1525 631 605 535 122 107 71
BglII A'GATC_T
Cuts at: 5738 5738
Size: 5743
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 2071 2129 2483 3804 4960 1790
Size: 281 58 354 1321 1156 2573
Fragments arranged by size:
2573 1321 1156 354 281 58
BmgBI CAC'GTC
Cuts at: 1318 2020 1318
Size: 702 5041
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2497 3038 3474
Size: 416 53 83 399 24 1080 541 436
Cuts at: 3474 4432 442
Size: 958 1753
Fragments arranged by size:
1753 1080 958 541 436 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2538 2538
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2657 4403 1239
Size: 1418 1746 2579
Fragments arranged by size:
2579 1746 1418
Bpu10I CC'TnA_GC
Cuts at: 2195 2457 2475 2547 2652 2195
Size: 262 18 72 105 5286
Fragments arranged by size:
5286 262 105 72 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2533 3868 4733 5031 2533
Size: 1335 865 298 3245
Fragments arranged by size:
3245 1335 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2088 2374 4406 882
Size: 617 589 286 2032 2219
Fragments arranged by size:
2219 2032 617 589 286
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 2280 2929 3550
Size: 53 83 186 936 699 48 649 621
Cuts at: 3550 3932 4567 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 699 649 635 621 382 186
83 53 48
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2087 2117 2591 2621 2907
Size: 30 165 30 684 30 474 30 286
Cuts at: 2907 2937 1178
Size: 30 3984
Fragments arranged by size:
3984 684 474 286 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2565 2593 2596 2738 2876 1186
Size: 1379 28 3 142 138 4053
Fragments arranged by size:
4053 1379 142 138 28 3
BseYI C'CCAG_C
Cuts at: 2352 2426 2687 4946 5442 2352
Size: 74 261 2259 496 2653
Fragments arranged by size:
2653 2259 496 261 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 2222 2297 1163
Size: 573 462 24 75 4609
Fragments arranged by size:
4609 573 462 75 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3804 3889 4960 3804
Size: 85 1071 4587
Fragments arranged by size:
4587 1071 85
BsiWI C'GTAC_G
Cuts at: 2707 2707
Size: 5743
BsmI GAATG_Cn'
Cuts at: 2586 3254 3347 2586
Size: 668 93 4982
Fragments arranged by size:
4982 668 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2920 2920
Size: 5743
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2129 2483 3062 3804 3889 4960
Size: 399 281 58 354 579 742 85 1071
Cuts at: 4960 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 579 399 354 281 85
58
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 3632 5362 3632
Size: 1730 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3098 844
Size: 2254 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4387 4574 1700
Size: 110 2577 187 2869
Fragments arranged by size:
2869 2577 187 110
BsrGI T'GTAC_A
Cuts at: 96 2773 96
Size: 2677 3066
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 2030 3803 4815 2030
Size: 1773 1012 2958
Fragments arranged by size:
2958 1773 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2994 794
Size: 2200 3543
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2470 2617 2011
Size: 459 147 5137
Fragments arranged by size:
5137 459 147
BstYI r'GATC_y
Cuts at: 1972 2491 3073 3440 3825 3842 5294 5380 5392
Size: 519 582 367 385 17 1452 86 12
Cuts at: 5392 5738 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 582 519 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2304 2304
Size: 5743
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 3259 4053 4073 3259
Size: 794 20 4929
Fragments arranged by size:
4929 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DraIII CAC_nnn'GTG
Cuts at: 2176 2176
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2440 3190 4079
Size: 54 1425 50 275 18 610 750 889
Cuts at: 4079 5708 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 750 610 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3673 1353
Size: 2320 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2398 2692 4330 4704 4850 387
Size: 1064 138 809 294 1638 374 146 1280
Fragments arranged by size:
1638 1280 1064 809 374 294 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2657 3806 4403 5190 5458 1239
Size: 393 1025 1149 597 787 268 1524
Fragments arranged by size:
1524 1149 1025 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2077 2303 2077
Size: 226 5517
EcoO109I rG'GnC_Cy
Cuts at: 2126 2288 2308 2126
Size: 162 20 5561
Fragments arranged by size:
5561 162 20
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 2202 2235 4890 1678
Size: 524 33 2655 2531
Fragments arranged by size:
2655 2531 524 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2384 2416 4384 4416 4458
Size: 32 135 32 812 32 1968 32 42
Cuts at: 4458 4490 1373
Size: 32 2626
Fragments arranged by size:
2626 1968 812 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3333 669
Size: 988 281 1149 246 3079
Fragments arranged by size:
3079 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KasI G'GCGC_C
Cuts at: 2231 2231
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4857 5041 1305
Size: 3552 184 2007
Fragments arranged by size:
3552 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3921
Fragments arranged by size:
3921 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2615 3701 4060 4219 518
Size: 648 1449 1086 359 159 2042
Fragments arranged by size:
2042 1449 1086 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2164 2229 2257 3159 3836 4984 5229 1606
Size: 558 65 28 902 677 1148 245 2120
Fragments arranged by size:
2120 1148 902 677 558 245 65 28
NarI GG'CG_CC
Cuts at: 2232 2232
Size: 5743
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NsiI A_TGCA'T
Cuts at: 2946 2946
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 3101 4646 1132
Size: 97 1872 1545 2229
Fragments arranged by size:
2229 1872 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2045 1108
Size: 937 4806
PfoI T'CCnGG_A
Cuts at: 2274 2343 2274
Size: 69 5674
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2288 2288
Size: 5743
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PspOMI G'GGCC_C
Cuts at: 2125 2125
Size: 5743
PspXI vC'TCGA_Gb
Cuts at: 2738 2738
Size: 5743
PstI C_TGCA'G
Cuts at: 830 2271 2334 2451 2736 3095 830
Size: 1441 63 117 285 359 3478
Fragments arranged by size:
3478 1441 359 285 117 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 3991 5703 3991
Size: 1712 4031
SfcI C'TryA_G
Cuts at: 826 1045 2267 2330 2447 2732 3091 4226 4907
Size: 219 1222 63 117 285 359 1135 681
Cuts at: 4907 5098 826
Size: 191 1471
Fragments arranged by size:
1471 1222 1135 681 359 285 219 191
117 63
SfoI GGC'GCC
Cuts at: 2233 2233
Size: 5743
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 3070 1872
Size: 1198 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2512 2738 3847 4748 5010 820
Size: 197 926 569 226 1109 901 262 1553
Fragments arranged by size:
1553 1109 926 901 569 262 226 197
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 2193 1517
Size: 676 5067
StyI C'CwwG_G
Cuts at: 513 1065 2004 3182 513
Size: 552 939 1178 3074
Fragments arranged by size:
3074 1178 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3770 3787 3940 4125 4570 325
Size: 1255 2190 17 153 185 445 1498
Fragments arranged by size:
2190 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2773 3989 5701
Size: 274 80 33 51 1443 796 1216 1712
Cuts at: 5701 96
Size: 138
Fragments arranged by size:
1712 1443 1216 796 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2087 2119 2907 2939 5703 5735 2087
Size: 32 788 32 2764 32 2095
Fragments arranged by size:
2764 2095 788 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3007 3007
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 2738 1943
Size: 795 4948
XmaI C'CCGG_G
Cuts at: 1870 3068 1870
Size: 1198 4545
XmnI GAAnn'nnTTC
Cuts at: 3872 3872
Size: 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2281 3551 276
Size: 53 83 186 936 747 1270 2468
Fragments arranged by size:
2468 1270 936 747 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BstZ17I EcoICRI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI
PmlI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI