(Circular) (Six-base) MAPSORT of: pFN21AE4065.seq Check: 1084 from: 1 to: 5743 pFN21AE4065 5743 bp With 182 enzymes: SgfI * March 28, 2012 13:09 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2283 3553 278 Size: 53 83 186 936 747 1270 2468 Fragments arranged by size: 2468 1270 936 747 186 83 53 AccI GT'mk_AC Cuts at: 1937 3086 1937 Size: 1149 4594 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3806 5190 5458 1632 Size: 2174 1384 268 1917 Fragments arranged by size: 2174 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 4642 5619 1203 Size: 3439 977 1327 Fragments arranged by size: 3439 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 4472 4472 Size: 5743 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5743 AlwNI CAG_nnn'CTG Cuts at: 2248 2311 2344 3486 5058 2248 Size: 63 33 1142 1572 2933 Fragments arranged by size: 2933 1572 1142 63 33 ApaI G_GGCC'C Cuts at: 2129 2129 Size: 5743 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 3052 3278 3430 5539 1493 Size: 1559 226 152 2109 1697 Fragments arranged by size: 2109 1697 1559 226 152 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 2738 2763 3068 1870 Size: 73 795 25 305 4545 Fragments arranged by size: 4545 795 305 73 25 BamHI G'GATC_C Cuts at: 3073 3440 3073 Size: 367 5376 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2231 3064 4519 618 Size: 325 217 627 279 165 833 1455 1842 Fragments arranged by size: 1842 1455 833 627 325 279 217 165 BanII G_rGCy'C Cuts at: 1391 2129 3062 1391 Size: 738 933 4072 Fragments arranged by size: 4072 933 738 BbeI G_GCGC'C Cuts at: 2235 2235 Size: 5743 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2830 928 Size: 971 931 3841 Fragments arranged by size: 3841 971 931 BbvCI CC'TCA_GC Cuts at: 2195 2457 2475 2547 2652 2195 Size: 262 18 72 105 5286 Fragments arranged by size: 5286 262 105 72 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3934 3968 1903 Size: 34 1997 34 3678 Fragments arranged by size: 3678 1997 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3635 4845 3635 Size: 1210 4533 BclI T'GATC_A Cuts at: 1548 1548 Size: 5743 BfrBI ATG'CAT Cuts at: 2944 2944 Size: 5743 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2207 4354 136 Size: 107 122 71 631 605 535 2147 1525 Fragments arranged by size: 2147 1525 631 605 535 122 107 71 BglII A'GATC_T Cuts at: 5738 5738 Size: 5743 BlpI GC'TnA_GC Cuts at: 3160 3160 Size: 5743 Bme1580I G_kGCm'C Cuts at: 1790 2071 2129 2483 3804 4960 1790 Size: 281 58 354 1321 1156 2573 Fragments arranged by size: 2573 1321 1156 354 281 58 BmgBI CAC'GTC Cuts at: 1318 2020 1318 Size: 702 5041 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2497 3038 3474 Size: 416 53 83 399 24 1080 541 436 Cuts at: 3474 4432 442 Size: 958 1753 Fragments arranged by size: 1753 1080 958 541 436 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5743 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2538 2538 Size: 5743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2657 4403 1239 Size: 1418 1746 2579 Fragments arranged by size: 2579 1746 1418 Bpu10I CC'TnA_GC Cuts at: 2195 2457 2475 2547 2652 2195 Size: 262 18 72 105 5286 Fragments arranged by size: 5286 262 105 72 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2533 3868 4733 5031 2533 Size: 1335 865 298 3245 Fragments arranged by size: 3245 1335 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2088 2374 4406 882 Size: 617 589 286 2032 2219 Fragments arranged by size: 2219 2032 617 589 286 BsaAI yAC'GTr Cuts at: 493 1204 3534 493 Size: 711 2330 2702 Fragments arranged by size: 2702 2330 711 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2232 2280 2929 3550 Size: 53 83 186 936 699 48 649 621 Cuts at: 3550 3932 4567 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 699 649 635 621 382 186 83 53 48 BsaWI w'CCGG_w Cuts at: 4176 4574 4848 4995 5406 4176 Size: 398 274 147 411 4513 Fragments arranged by size: 4513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2087 2117 2591 2621 2907 Size: 30 165 30 684 30 474 30 286 Cuts at: 2907 2937 1178 Size: 30 3984 Fragments arranged by size: 3984 684 474 286 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2565 2593 2596 2738 2876 1186 Size: 1379 28 3 142 138 4053 Fragments arranged by size: 4053 1379 142 138 28 3 BseYI C'CCAG_C Cuts at: 2352 2426 2687 4946 5442 2352 Size: 74 261 2259 496 2653 Fragments arranged by size: 2653 2259 496 261 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 2222 2297 1163 Size: 573 462 24 75 4609 Fragments arranged by size: 4609 573 462 75 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3193 3954 4982 1540 Size: 275 186 1192 761 1028 2301 Fragments arranged by size: 2301 1192 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3804 3889 4960 3804 Size: 85 1071 4587 Fragments arranged by size: 4587 1071 85 BsiWI C'GTAC_G Cuts at: 2707 2707 Size: 5743 BsmI GAATG_Cn' Cuts at: 2586 3254 3347 2586 Size: 668 93 4982 Fragments arranged by size: 4982 668 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2920 2920 Size: 5743 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2129 2483 3062 3804 3889 4960 Size: 399 281 58 354 579 742 85 1071 Cuts at: 4960 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 579 399 354 281 85 58 BspEI T'CCGG_A Cuts at: 5406 5406 Size: 5743 BspHI T'CATG_A Cuts at: 3632 5362 3632 Size: 1730 4013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3098 844 Size: 2254 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3630 1394 Size: 429 1807 3507 Fragments arranged by size: 3507 1807 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4238 4412 65 Size: 1183 2990 174 1396 Fragments arranged by size: 2990 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4387 4574 1700 Size: 110 2577 187 2869 Fragments arranged by size: 2869 2577 187 110 BsrGI T'GTAC_A Cuts at: 96 2773 96 Size: 2677 3066 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5743 BssSI C'ACGA_G Cuts at: 2030 3803 4815 2030 Size: 1773 1012 2958 Fragments arranged by size: 2958 1773 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2994 794 Size: 2200 3543 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2470 2617 2011 Size: 459 147 5137 Fragments arranged by size: 5137 459 147 BstYI r'GATC_y Cuts at: 1972 2491 3073 3440 3825 3842 5294 5380 5392 Size: 519 582 367 385 17 1452 86 12 Cuts at: 5392 5738 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 582 519 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2304 2304 Size: 5743 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4252 Fragments arranged by size: 4252 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5004 Fragments arranged by size: 5004 683 56 BtsI GCAGTG_nn' Cuts at: 3259 4053 4073 3259 Size: 794 20 4929 Fragments arranged by size: 4929 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DraIII CAC_nnn'GTG Cuts at: 2176 2176 Size: 5743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4750 809 Size: 507 3434 1802 Fragments arranged by size: 3434 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2440 3190 4079 Size: 54 1425 50 275 18 610 750 889 Cuts at: 4079 5708 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 750 610 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3190 1537 Size: 275 1378 4090 Fragments arranged by size: 4090 1378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3673 1353 Size: 2320 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2398 2692 4330 4704 4850 387 Size: 1064 138 809 294 1638 374 146 1280 Fragments arranged by size: 1638 1280 1064 809 374 294 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2657 3806 4403 5190 5458 1239 Size: 393 1025 1149 597 787 268 1524 Fragments arranged by size: 1524 1149 1025 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2077 2303 2077 Size: 226 5517 EcoO109I rG'GnC_Cy Cuts at: 2126 2288 2308 2126 Size: 162 20 5561 Fragments arranged by size: 5561 162 20 EcoRI G'AATT_C Cuts at: 3052 3430 3052 Size: 378 5365 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 2202 2235 4890 1678 Size: 524 33 2655 2531 Fragments arranged by size: 2655 2531 524 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2384 2416 4384 4416 4458 Size: 32 135 32 812 32 1968 32 42 Cuts at: 4458 4490 1373 Size: 32 2626 Fragments arranged by size: 2626 1968 812 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3087 3333 669 Size: 988 281 1149 246 3079 Fragments arranged by size: 3079 1149 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KasI G'GCGC_C Cuts at: 2231 2231 Size: 5743 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4857 5041 1305 Size: 3552 184 2007 Fragments arranged by size: 3552 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3921 Fragments arranged by size: 3921 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2615 3701 4060 4219 518 Size: 648 1449 1086 359 159 2042 Fragments arranged by size: 2042 1449 1086 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2164 2229 2257 3159 3836 4984 5229 1606 Size: 558 65 28 902 677 1148 245 2120 Fragments arranged by size: 2120 1148 902 677 558 245 65 28 NarI GG'CG_CC Cuts at: 2232 2232 Size: 5743 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4252 Fragments arranged by size: 4252 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5743 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NsiI A_TGCA'T Cuts at: 2946 2946 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 3101 4646 1132 Size: 97 1872 1545 2229 Fragments arranged by size: 2229 1872 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5743 PciI A'CATG_T Cuts at: 4642 4642 Size: 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 2045 1108 Size: 937 4806 PfoI T'CCnGG_A Cuts at: 2274 2343 2274 Size: 69 5674 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2288 2288 Size: 5743 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PspOMI G'GGCC_C Cuts at: 2125 2125 Size: 5743 PspXI vC'TCGA_Gb Cuts at: 2738 2738 Size: 5743 PstI C_TGCA'G Cuts at: 830 2271 2334 2451 2736 3095 830 Size: 1441 63 117 285 359 3478 Fragments arranged by size: 3478 1441 359 285 117 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 3991 5703 3991 Size: 1712 4031 SfcI C'TryA_G Cuts at: 826 1045 2267 2330 2447 2732 3091 4226 4907 Size: 219 1222 63 117 285 359 1135 681 Cuts at: 4907 5098 826 Size: 191 1471 Fragments arranged by size: 1471 1222 1135 681 359 285 219 191 117 63 SfoI GGC'GCC Cuts at: 2233 2233 Size: 5743 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 3070 1872 Size: 1198 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2512 2738 3847 4748 5010 820 Size: 197 926 569 226 1109 901 262 1553 Fragments arranged by size: 1553 1109 926 901 569 262 226 197 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 3101 1132 Size: 1969 3774 SspI AAT'ATT Cuts at: 5 52 3667 5 Size: 47 3615 2081 Fragments arranged by size: 3615 2081 47 StuI AGG'CCT Cuts at: 1517 2193 1517 Size: 676 5067 StyI C'CwwG_G Cuts at: 513 1065 2004 3182 513 Size: 552 939 1178 3074 Fragments arranged by size: 3074 1178 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3770 3787 3940 4125 4570 325 Size: 1255 2190 17 153 185 445 1498 Fragments arranged by size: 2190 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2773 3989 5701 Size: 274 80 33 51 1443 796 1216 1712 Cuts at: 5701 96 Size: 138 Fragments arranged by size: 1712 1443 1216 796 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2087 2119 2907 2939 5703 5735 2087 Size: 32 788 32 2764 32 2095 Fragments arranged by size: 2764 2095 788 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5743 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3007 3007 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 2738 1943 Size: 795 4948 XmaI C'CCGG_G Cuts at: 1870 3068 1870 Size: 1198 4545 XmnI GAAnn'nnTTC Cuts at: 3872 3872 Size: 5743 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2281 3551 276 Size: 53 83 186 936 747 1270 2468 Fragments arranged by size: 2468 1270 936 747 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI